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The map label for this gene is trmB

Identifier: 86747722

GI number: 86747722

Start: 668865

End: 669635

Strand: Direct

Name: trmB

Synonym: RPB_0596

Alternate gene names: 86747722

Gene position: 668865-669635 (Clockwise)

Preceding gene: 86747721

Following gene: 86747723

Centisome position: 12.55

GC content: 66.67

Gene sequence:

>771_bases
ATGGTCACTATTGATCGTCGCGTGCAGCCGATTGCGCATCTCGCGTGCAACCATCAACGAGCGTTCATCGACATGAATGA
ATCGGTTCATCATCAGGGCTCGTTTTTCGGGCGGCGCAAGGGCCACAAGCTTCGCGCGCATCAGGCCGATCTGGTCGACA
AGCTGCTGCCGCATCTGGCGCTGGCGATCGACGGGCCCGCGCCGGCCTCGCTGGTCGAGCTGTTCGATCCGCCGGTCGAG
GCGGTGCGGCTGGAGATCGGCTTCGGCGGCGGCGAGCATCTGATCGCCGAGGCGCTGGCGCATCCGTCGACCGGGTTCAT
CGGCGCCGAGCCCTATGTCAACGGCATGGCCAAGATCCTGGCCCGGATCGAACAGGAGAACATCCGCAACATCCGCTTGT
TCGCCGGCGATGCGGCGGCGCTGATGGCGTGGGTGCCGGCGCACGATTTGAATCGAATTGATCTCATTCACCCGGACCCG
TGGCCGAAGCGGCGGCACTGGAAGCGGCGCTTCGTGCAGGACGCGATGGTCGGCGCGATGGCGCGGGCGCTGCGCGCGAA
CGGCGAATTCCGCTTCGTCAGCGACATCGACAGCTACAATGCATGGACGCTGGCGCATCTGCTGCGCGCGCCGGATTTCG
ACTGGACCGCCGAGCGCGCCGACGACTGGCGCAAGCCGTGGCCGAACTACACGATGACGCGCTACGGCCGCAAAGCCGAA
CGCGAGGGCCGCCGCGCCGCCTATCTGCGGTTCCGCAGGCGGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGATCCGTGCGCTGAACTAATTCAGTGCCACGGATCGCGGCGATTTGTTTGTGTCGTGGGTTGAACTAATTCCGACTCTG
CACGTCATGCGGGCTGTCGT

Downstream 100 bases:

>100_bases
GACGCGGCCGCTGCTCCCCAACGAGTCGTCCCCGCGCAGGCCAGCGCAATCGCCTCTGGACGTCGAGGCTATTGCACCGG
GCCTCCTCACCCTCCCCTGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVTIDRRVQPIAHLACNHQRAFIDMNESVHHQGSFFGRRKGHKLRAHQADLVDKLLPHLALAIDGPAPASLVELFDPPVE
AVRLEIGFGGGEHLIAEALAHPSTGFIGAEPYVNGMAKILARIEQENIRNIRLFAGDAAALMAWVPAHDLNRIDLIHPDP
WPKRRHWKRRFVQDAMVGAMARALRANGEFRFVSDIDSYNAWTLAHLLRAPDFDWTAERADDWRKPWPNYTMTRYGRKAE
REGRRAAYLRFRRRAA

Sequences:

>Translated_256_residues
MVTIDRRVQPIAHLACNHQRAFIDMNESVHHQGSFFGRRKGHKLRAHQADLVDKLLPHLALAIDGPAPASLVELFDPPVE
AVRLEIGFGGGEHLIAEALAHPSTGFIGAEPYVNGMAKILARIEQENIRNIRLFAGDAAALMAWVPAHDLNRIDLIHPDP
WPKRRHWKRRFVQDAMVGAMARALRANGEFRFVSDIDSYNAWTLAHLLRAPDFDWTAERADDWRKPWPNYTMTRYGRKAE
REGRRAAYLRFRRRAA
>Mature_256_residues
MVTIDRRVQPIAHLACNHQRAFIDMNESVHHQGSFFGRRKGHKLRAHQADLVDKLLPHLALAIDGPAPASLVELFDPPVE
AVRLEIGFGGGEHLIAEALAHPSTGFIGAEPYVNGMAKILARIEQENIRNIRLFAGDAAALMAWVPAHDLNRIDLIHPDP
WPKRRHWKRRFVQDAMVGAMARALRANGEFRFVSDIDSYNAWTLAHLLRAPDFDWTAERADDWRKPWPNYTMTRYGRKAE
REGRRAAYLRFRRRAA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=173, Percent_Identity=30.635838150289, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_RHOP2 (Q2J2K3)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484218.1
- ProteinModelPortal:   Q2J2K3
- SMR:   Q2J2K3
- STRING:   Q2J2K3
- GeneID:   3908289
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0596
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RYGRKAE
- ProtClustDB:   PRK00121
- BioCyc:   RPAL316058:RPB_0596-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29242; Mature: 29242

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: BINDING 85-85 BINDING 110-110 BINDING 137-137 BINDING 159-159 BINDING 163-163 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIDRRVQPIAHLACNHQRAFIDMNESVHHQGSFFGRRKGHKLRAHQADLVDKLLPHLA
CCCCCHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHE
LAIDGPAPASLVELFDPPVEAVRLEIGFGGGEHLIAEALAHPSTGFIGAEPYVNGMAKIL
EEECCCCCHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ARIEQENIRNIRLFAGDAAALMAWVPAHDLNRIDLIHPDPWPKRRHWKRRFVQDAMVGAM
HHHHHHCCCEEEEEECCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ARALRANGEFRFVSDIDSYNAWTLAHLLRAPDFDWTAERADDWRKPWPNYTMTRYGRKAE
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHCCHHH
REGRRAAYLRFRRRAA
HCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVTIDRRVQPIAHLACNHQRAFIDMNESVHHQGSFFGRRKGHKLRAHQADLVDKLLPHLA
CCCCCHHHHHHHHHHHCCCEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHE
LAIDGPAPASLVELFDPPVEAVRLEIGFGGGEHLIAEALAHPSTGFIGAEPYVNGMAKIL
EEECCCCCHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ARIEQENIRNIRLFAGDAAALMAWVPAHDLNRIDLIHPDPWPKRRHWKRRFVQDAMVGAM
HHHHHHCCCEEEEEECCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ARALRANGEFRFVSDIDSYNAWTLAHLLRAPDFDWTAERADDWRKPWPNYTMTRYGRKAE
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHCCHHH
REGRRAAYLRFRRRAA
HCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA