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The map label for this gene is pcaF [H]

Identifier: 86747651

GI number: 86747651

Start: 586052

End: 587260

Strand: Reverse

Name: pcaF [H]

Synonym: RPB_0525

Alternate gene names: 86747651

Gene position: 587260-586052 (Counterclockwise)

Preceding gene: 86747652

Following gene: 86747650

Centisome position: 11.01

GC content: 69.07

Gene sequence:

>1209_bases
ATGGCCGACGTCTTCATCTGCGACGCAGTGCGCACCCCAATCGGCCGGTTCGGCGGATCTCTCGCCAAAGTCCGTGCCGA
CGACCTCGCCGCGGTTCCGATCAAGGCGCTGATGGCCAAGCACCCCGATCTCGACTGGAGCGCGGTGGACGAGGTGTTCT
TCGGCTGCGCCAACCAGGCCGGCGAAGACAACCGCAACGTCGCCCGGATGGCGACGCTGCTCGCGGGCCTGCCGGATTCG
GTGCCGGCCCAGACCCTCAACCGGCTGTGCGCCTCCGGGCTCGACGCGGTCGGCGCCGCGGGCCGCGCGATCCGCGCCGG
CGAGATCGATCTGGCGATTGCCGGCGGCGTCGAATCGATGACACGGGCGCCGTTCGTGATGGGCAAAGCCGGCGAGGCGT
TTTCCCGCCAGGCGGACATCTTCGACACCACGATCGGCTGGCGTTTCATCAATCCGCTGATGAAGGCGCAATACGGTGTC
GACGCGATGCCGGAGACCGGCGAGAACGTCGCCGAGGAATTCCAGATTTCGCGCGCCGATCAGGACGCCTTCGCGATCCG
ATCCCAGCAGCGCGCAGGCGCCGCCATCGCCGCCGGTTACTTCGCGCAGGAGATCGCGCCGGTGTCGGCGCCGGGCGGCA
AGGCCGGTCCGATCATCGTCGACAAGGACGAGCATCCGCGCCCGGAGACGACGCTGGAAGGCCTCGCCAAGCTGAAGCCG
ATCGTGCGCAATCCCGGCACGGTGACCGCCGGCAACGCCTCGGGCGTCAATGACGGCGCTGCGGCGATGATCGTGGCCTC
GGAGGCTGCGGTGAAGAAACACGGCCTGACGCCCCGGGCGAAGATTCTCGGCCTCGCCTCGGCGGCAGTGCCGCCGCGCA
TCATGGGCATCGGCCCGGTGCCGGCGACCCGCAAGCTGATGGAGCGGCTGGGGCTGAAGATCTCCGACTTCGACCTGATC
GAGCTCAACGAAGCCTTCGCCTCGCAGGGCATCGCCTGCCTGCGCCAGCTCGGCGTCGCCGACGATGCCGATTTCGTCAA
TCCGCATGGTGGCGCGATCGCGCTCGGCCACCCGCTCGGCATGAGCGGCACGCGGCTGGCGCTGACGGCGGTGCACGGCA
TGGAAGCCCGCGGCGGCAAATTGGCGCTGGCGACGATGTGCGTCGGCGTCGGCCAGGGCGTCGCGATGGCGATCGAGAAA
CTGAACTAA

Upstream 100 bases:

>100_bases
TCGCGACGGCTGAGCGTGCATTGACCACGTCGGCCGGCCTTCCTAAGAGTGCCTTTCAATTCCGGCCCTCAGCCCGGAAT
CGCCAGCAAGGGAGAGAACC

Downstream 100 bases:

>100_bases
GCATCTGCGGCGGGGGCGTCTCGCACGGGCCGGAACCGGCGCGGCGCCCCGACCGCTTCGCATTCCGCAACAGCCCCGCG
TTCCGCAAGACTTGTGCCGG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MADVFICDAVRTPIGRFGGSLAKVRADDLAAVPIKALMAKHPDLDWSAVDEVFFGCANQAGEDNRNVARMATLLAGLPDS
VPAQTLNRLCASGLDAVGAAGRAIRAGEIDLAIAGGVESMTRAPFVMGKAGEAFSRQADIFDTTIGWRFINPLMKAQYGV
DAMPETGENVAEEFQISRADQDAFAIRSQQRAGAAIAAGYFAQEIAPVSAPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
IVRNPGTVTAGNASGVNDGAAAMIVASEAAVKKHGLTPRAKILGLASAAVPPRIMGIGPVPATRKLMERLGLKISDFDLI
ELNEAFASQGIACLRQLGVADDADFVNPHGGAIALGHPLGMSGTRLALTAVHGMEARGGKLALATMCVGVGQGVAMAIEK
LN

Sequences:

>Translated_402_residues
MADVFICDAVRTPIGRFGGSLAKVRADDLAAVPIKALMAKHPDLDWSAVDEVFFGCANQAGEDNRNVARMATLLAGLPDS
VPAQTLNRLCASGLDAVGAAGRAIRAGEIDLAIAGGVESMTRAPFVMGKAGEAFSRQADIFDTTIGWRFINPLMKAQYGV
DAMPETGENVAEEFQISRADQDAFAIRSQQRAGAAIAAGYFAQEIAPVSAPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
IVRNPGTVTAGNASGVNDGAAAMIVASEAAVKKHGLTPRAKILGLASAAVPPRIMGIGPVPATRKLMERLGLKISDFDLI
ELNEAFASQGIACLRQLGVADDADFVNPHGGAIALGHPLGMSGTRLALTAVHGMEARGGKLALATMCVGVGQGVAMAIEK
LN
>Mature_401_residues
ADVFICDAVRTPIGRFGGSLAKVRADDLAAVPIKALMAKHPDLDWSAVDEVFFGCANQAGEDNRNVARMATLLAGLPDSV
PAQTLNRLCASGLDAVGAAGRAIRAGEIDLAIAGGVESMTRAPFVMGKAGEAFSRQADIFDTTIGWRFINPLMKAQYGVD
AMPETGENVAEEFQISRADQDAFAIRSQQRAGAAIAAGYFAQEIAPVSAPGGKAGPIIVDKDEHPRPETTLEGLAKLKPI
VRNPGTVTAGNASGVNDGAAAMIVASEAAVKKHGLTPRAKILGLASAAVPPRIMGIGPVPATRKLMERLGLKISDFDLIE
LNEAFASQGIACLRQLGVADDADFVNPHGGAIALGHPLGMSGTRLALTAVHGMEARGGKLALATMCVGVGQGVAMAIEKL
N

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=42.9292929292929, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=39.5989974937343, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=39.3120393120393, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=36.3861386138614, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4504327, Length=432, Percent_Identity=31.25, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI194353979, Length=400, Percent_Identity=28.75, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=64.3391521197007, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=46.039603960396, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=44.5273631840796, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=41.6256157635468, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788683, Length=425, Percent_Identity=31.5294117647059, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=41, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI25147385, Length=403, Percent_Identity=39.2059553349876, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17535921, Length=411, Percent_Identity=34.3065693430657, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=32.1759259259259, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=32.7543424317618, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322031, Length=409, Percent_Identity=36.4303178484108, Blast_Score=235, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=38.2716049382716, Blast_Score=222, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=42.2885572139304, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=38.4615384615385, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=35.4679802955665, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=31.7757009345794, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI19921506, Length=360, Percent_Identity=25.8333333333333, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24585051, Length=360, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41547; Mature: 41416

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVFICDAVRTPIGRFGGSLAKVRADDLAAVPIKALMAKHPDLDWSAVDEVFFGCANQA
CCCEEEEHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMATLLAGLPDSVPAQTLNRLCASGLDAVGAAGRAIRAGEIDLAIAGGVESM
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHCCCCEEECCCEEEEEECCHHHH
TRAPFVMGKAGEAFSRQADIFDTTIGWRFINPLMKAQYGVDAMPETGENVAEEFQISRAD
HHCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
QDAFAIRSQQRAGAAIAAGYFAQEIAPVSAPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
CHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
IVRNPGTVTAGNASGVNDGAAAMIVASEAAVKKHGLTPRAKILGLASAAVPPRIMGIGPV
HHCCCCEEECCCCCCCCCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCC
PATRKLMERLGLKISDFDLIELNEAFASQGIACLRQLGVADDADFVNPHGGAIALGHPLG
CHHHHHHHHHCCEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGTRLALTAVHGMEARGGKLALATMCVGVGQGVAMAIEKLN
CCCCEEEEEEECCCCCCCCEEEHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
ADVFICDAVRTPIGRFGGSLAKVRADDLAAVPIKALMAKHPDLDWSAVDEVFFGCANQA
CCEEEEHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMATLLAGLPDSVPAQTLNRLCASGLDAVGAAGRAIRAGEIDLAIAGGVESM
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHCCCCEEECCCEEEEEECCHHHH
TRAPFVMGKAGEAFSRQADIFDTTIGWRFINPLMKAQYGVDAMPETGENVAEEFQISRAD
HHCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
QDAFAIRSQQRAGAAIAAGYFAQEIAPVSAPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
CHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
IVRNPGTVTAGNASGVNDGAAAMIVASEAAVKKHGLTPRAKILGLASAAVPPRIMGIGPV
HHCCCCEEECCCCCCCCCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCC
PATRKLMERLGLKISDFDLIELNEAFASQGIACLRQLGVADDADFVNPHGGAIALGHPLG
CHHHHHHHHHCCEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGTRLALTAVHGMEARGGKLALATMCVGVGQGVAMAIEKLN
CCCCEEEEEEECCCCCCCCEEEHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]