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The map label for this gene is ispG [H]

Identifier: 86747648

GI number: 86747648

Start: 582955

End: 584250

Strand: Reverse

Name: ispG [H]

Synonym: RPB_0522

Alternate gene names: 86747648

Gene position: 584250-582955 (Counterclockwise)

Preceding gene: 86747649

Following gene: 86747647

Centisome position: 10.96

GC content: 67.52

Gene sequence:

>1296_bases
ATGAACAAGCTCGAAAATCCGCTGCAGAACGACGTCGCCGGCCCCGCGCCGCGGCACAGGACCACCCAGGTCATGGTCGG
CGATATCGCCGTCGGCGGCGGTGCGCCGATCGTCGTGCAGTCGATGACCAACACCGACACCGCCGACATCGACGGCACCA
TCAAGCAGGTCGCGGCGCTGGCGCGCGCCGGCTCCGAGATGGTCCGGATCACGGTCGACCGCGAGGAAGCCGCCGCCGCC
GTGCCGCATATCCGCGACGGCCTGCGCAAGCTCGGCCTGACCACGCCCTTGATCGGCGACTTCCATTACATCGGCCACAA
GCTGCTCGCCGAGTATCCGGCCTGCGCCGAAGCGCTCGACAAATACCGGATCAATCCGGGCAATGTCGGCTTCAAGGCCA
AGCGCGACACCCAGTTCGCCGACATCGTCGAGATCGCCATCAAGAACAACAAGGCGGTCCGGATCGGCGCCAATTGGGGT
TCGCTGGATCAGGAGCTGCTGACCAAGCTGATGGACGAGAACGCCGCCTCCGCCCAGCCGCGCGACGTCCGCGCCGTGAC
CCGCGAGGCGATGGTGCAGTCGGCGCTGCTGTCGGCCGCCCGCGCCGAAGAGATCGGCCTGCCGAAGACCAAGATGGTGC
TGTCCGCCAAGGTGTCGGCGGTGCAGGACCTGATCGCGGTGTATCAGGATCTGGCGTCGCGCTCCGACTACGCCATTCAT
CTCGGTCTCACCGAAGCCGGCATGGGCTCCAAGGGCATTGTCGCCTCCTCGGCCGCGCTCGGCATCCTGCTGCAGCAGGG
CATCGGCGACACCATCCGGATTTCGCTGACGCCGGAGCCCGGCGGCGACCGGACGCTCGAAGTCCAGGTCGCGCAGGAAT
TGCTGCAGACCATGGGCTTCCGCACCTTCGTGCCGCTGGTGGCGGCCTGCCCGGGCTGCGGCCGCACCACCTCGACCACG
TTCCAGGAACTGGCGCGCTCGATCCAGGATTTCATCCGCGTCGAGATGCCGAGCTGGAAGACCCGCTATCCGGGCGTCGA
GAACCTCAACGTCGCGGTGATGGGCTGCATCGTCAACGGCCCCGGCGAGAGCAAGCACGCCAATATCGGCATCTCGCTGC
CCGGCACCGGCGAAAGCCCGGCGGCGCCGGTGTTCGTCGACGGCGAAAAATTCCGCACCCTGCGCGGCGAGAACATCGCC
GGCGACTTCAAGGCGCTGGTGATCGACTACATCGAACAGCGCTACGGCGCAGCGCCGAAGCCCGGCGCCGCCCAGATGGT
GCCGGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
GGATCTTGCGGTTCCGGGCGGCGGTGGCCAAGTCATCCCGGGGTTCCTGCACCCATTGATCCATTGCTGACTGTCACCAA
AACCTGATATTCCGGACGCC

Downstream 100 bases:

>100_bases
TACGCCTCTCCCGTACAATCACTTTGATCTTGCTCTCTGCGGTCATTTGGCCGCGGCGCTAGGATTATGTTAACCACAAA
AGAACAAAATCAGAACGATT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNKLENPLQNDVAGPAPRHRTTQVMVGDIAVGGGAPIVVQSMTNTDTADIDGTIKQVAALARAGSEMVRITVDREEAAAA
VPHIRDGLRKLGLTTPLIGDFHYIGHKLLAEYPACAEALDKYRINPGNVGFKAKRDTQFADIVEIAIKNNKAVRIGANWG
SLDQELLTKLMDENAASAQPRDVRAVTREAMVQSALLSAARAEEIGLPKTKMVLSAKVSAVQDLIAVYQDLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTT
FQELARSIQDFIRVEMPSWKTRYPGVENLNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGEKFRTLRGENIA
GDFKALVIDYIEQRYGAAPKPGAAQMVPAAE

Sequences:

>Translated_431_residues
MNKLENPLQNDVAGPAPRHRTTQVMVGDIAVGGGAPIVVQSMTNTDTADIDGTIKQVAALARAGSEMVRITVDREEAAAA
VPHIRDGLRKLGLTTPLIGDFHYIGHKLLAEYPACAEALDKYRINPGNVGFKAKRDTQFADIVEIAIKNNKAVRIGANWG
SLDQELLTKLMDENAASAQPRDVRAVTREAMVQSALLSAARAEEIGLPKTKMVLSAKVSAVQDLIAVYQDLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTT
FQELARSIQDFIRVEMPSWKTRYPGVENLNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGEKFRTLRGENIA
GDFKALVIDYIEQRYGAAPKPGAAQMVPAAE
>Mature_431_residues
MNKLENPLQNDVAGPAPRHRTTQVMVGDIAVGGGAPIVVQSMTNTDTADIDGTIKQVAALARAGSEMVRITVDREEAAAA
VPHIRDGLRKLGLTTPLIGDFHYIGHKLLAEYPACAEALDKYRINPGNVGFKAKRDTQFADIVEIAIKNNKAVRIGANWG
SLDQELLTKLMDENAASAQPRDVRAVTREAMVQSALLSAARAEEIGLPKTKMVLSAKVSAVQDLIAVYQDLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTT
FQELARSIQDFIRVEMPSWKTRYPGVENLNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGEKFRTLRGENIA
GDFKALVIDYIEQRYGAAPKPGAAQMVPAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=399, Percent_Identity=32.3308270676692, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45818; Mature: 45818

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLENPLQNDVAGPAPRHRTTQVMVGDIAVGGGAPIVVQSMTNTDTADIDGTIKQVAAL
CCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHH
ARAGSEMVRITVDREEAAAAVPHIRDGLRKLGLTTPLIGDFHYIGHKLLAEYPACAEALD
HHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
KYRINPGNVGFKAKRDTQFADIVEIAIKNNKAVRIGANWGSLDQELLTKLMDENAASAQP
HCCCCCCCCCCEECCCCHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCC
RDVRAVTREAMVQSALLSAARAEEIGLPKTKMVLSAKVSAVQDLIAVYQDLASRSDYAIH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGF
EECEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCH
RTFVPLVAACPGCGRTTSTTFQELARSIQDFIRVEMPSWKTRYPGVENLNVAVMGCIVNG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEECC
PGESKHANIGISLPGTGESPAAPVFVDGEKFRTLRGENIAGDFKALVIDYIEQRYGAAPK
CCCCCCCEEEEECCCCCCCCCCCEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
PGAAQMVPAAE
CCCCCCCCCCC
>Mature Secondary Structure
MNKLENPLQNDVAGPAPRHRTTQVMVGDIAVGGGAPIVVQSMTNTDTADIDGTIKQVAAL
CCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHH
ARAGSEMVRITVDREEAAAAVPHIRDGLRKLGLTTPLIGDFHYIGHKLLAEYPACAEALD
HHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
KYRINPGNVGFKAKRDTQFADIVEIAIKNNKAVRIGANWGSLDQELLTKLMDENAASAQP
HCCCCCCCCCCEECCCCHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCC
RDVRAVTREAMVQSALLSAARAEEIGLPKTKMVLSAKVSAVQDLIAVYQDLASRSDYAIH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGF
EECEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCH
RTFVPLVAACPGCGRTTSTTFQELARSIQDFIRVEMPSWKTRYPGVENLNVAVMGCIVNG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEECC
PGESKHANIGISLPGTGESPAAPVFVDGEKFRTLRGENIAGDFKALVIDYIEQRYGAAPK
CCCCCCCEEEEECCCCCCCCCCCEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
PGAAQMVPAAE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA