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The map label for this gene is ligT [C]

Identifier: 86747409

GI number: 86747409

Start: 320215

End: 320781

Strand: Reverse

Name: ligT [C]

Synonym: RPB_0283

Alternate gene names: 86747409

Gene position: 320781-320215 (Counterclockwise)

Preceding gene: 86747410

Following gene: 86747408

Centisome position: 6.02

GC content: 65.96

Gene sequence:

>567_bases
ATGCCGCGTTTGTTCACTGGACTGGAGATTCCCGCCGACGTCACCCGGACCCTGTCTCATCTCCGCGGCGGCCTGCCCGG
CGCGCGCTGGGTCGATCCGGAAAACTATCACCTCACGCTGCGCTTCATCGGCGACATCGACGGTGTCACCGCCAACGAAA
TCGCCTCGACGCTGTTTCGGATCAACCGCAAACCGTTCGACGTGCAATTGCAGGGGCTGGCGAGCTTCGGCGGCCGCAAG
CCGCGCGCGGTGGTGGCGTCGGTGGTGCCGTCGAAACCTTTAGTCGAACTGCAGGCCGAACTGGAGCGGCTGCTGCGGCG
GGTCGGGCTCGATCCCGAAGGCCGCAAATTCACCCCGCACGTCACGCTGGCCTGGCTGCGCGACGCCTCCTGCCAGGACG
TCGCCGATTATCTGTCGCTCCGCGGCTATTTCCCCGCCAAGGTGTTCAAGGCCGAGCGCTTCGTGCTGTATTCGTCCCGC
GCCTCGACCGGCGGCGGGCCGTATGTGGTCGAGGCGCCGTACGCGCTCAAACACACCGAAGAGACAAGGGCCGTCCGAAC
TGCATAG

Upstream 100 bases:

>100_bases
ACAGAATTGGTGAGTTGATTCGCCGGACGTTTTGCGGAGTGCAAAGTCCCGAACGACAGGGACGGCGACCGCAATCGGGC
CTCAGACCGGGGGAGTTCCG

Downstream 100 bases:

>100_bases
CCGCCAGCAAGAATCCCGGCTTGCATTTTGTCGCAGGCTGAGGCGGTAAGGGCGTCCATGTCCCCGCCGCTCTCGTTTCG
TCAACATTATCAGTCCCTGG

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MPRLFTGLEIPADVTRTLSHLRGGLPGARWVDPENYHLTLRFIGDIDGVTANEIASTLFRINRKPFDVQLQGLASFGGRK
PRAVVASVVPSKPLVELQAELERLLRRVGLDPEGRKFTPHVTLAWLRDASCQDVADYLSLRGYFPAKVFKAERFVLYSSR
ASTGGGPYVVEAPYALKHTEETRAVRTA

Sequences:

>Translated_188_residues
MPRLFTGLEIPADVTRTLSHLRGGLPGARWVDPENYHLTLRFIGDIDGVTANEIASTLFRINRKPFDVQLQGLASFGGRK
PRAVVASVVPSKPLVELQAELERLLRRVGLDPEGRKFTPHVTLAWLRDASCQDVADYLSLRGYFPAKVFKAERFVLYSSR
ASTGGGPYVVEAPYALKHTEETRAVRTA
>Mature_187_residues
PRLFTGLEIPADVTRTLSHLRGGLPGARWVDPENYHLTLRFIGDIDGVTANEIASTLFRINRKPFDVQLQGLASFGGRKP
RAVVASVVPSKPLVELQAELERLLRRVGLDPEGRKFTPHVTLAWLRDASCQDVADYLSLRGYFPAKVFKAERFVLYSSRA
STGGGPYVVEAPYALKHTEETRAVRTA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20875; Mature: 20744

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTGLEIPADVTRTLSHLRGGLPGARWVDPENYHLTLRFIGDIDGVTANEIASTLFR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
INRKPFDVQLQGLASFGGRKPRAVVASVVPSKPLVELQAELERLLRRVGLDPEGRKFTPH
HCCCCCEEEEEEHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCC
VTLAWLRDASCQDVADYLSLRGYFPAKVFKAERFVLYSSRASTGGGPYVVEAPYALKHTE
EEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHEEEEEECCCCCCCCCEEEECCHHHHCCH
ETRAVRTA
HHHHCCCC
>Mature Secondary Structure 
PRLFTGLEIPADVTRTLSHLRGGLPGARWVDPENYHLTLRFIGDIDGVTANEIASTLFR
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHH
INRKPFDVQLQGLASFGGRKPRAVVASVVPSKPLVELQAELERLLRRVGLDPEGRKFTPH
HCCCCCEEEEEEHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCC
VTLAWLRDASCQDVADYLSLRGYFPAKVFKAERFVLYSSRASTGGGPYVVEAPYALKHTE
EEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHEEEEEECCCCCCCCCEEEECCHHHHCCH
ETRAVRTA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]