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The map label for this gene is mdh1 [H]

Identifier: 86747407

GI number: 86747407

Start: 317818

End: 318786

Strand: Reverse

Name: mdh1 [H]

Synonym: RPB_0281

Alternate gene names: 86747407

Gene position: 318786-317818 (Counterclockwise)

Preceding gene: 86747408

Following gene: 86747406

Centisome position: 5.98

GC content: 65.33

Gene sequence:

>969_bases
ATGGCGCGCGACAAGATTGCTTTGATTGGCTCCGGCCAGATCGGCGGAACGCTGGCTCACATGATCGGGCTGAAAGAGCT
CGGCGACGTGGTGCTGTTCGATATCGCCGAGGGCGTGCCGCAGGGTAAGGCGCTCGACATCGCGGAATCCTCGCCGGTCG
ACGGCTTCGACGCCAACTTCACCGGCGCCAATTCCTACGAGGCGATCGAAGGCGCGAGCGTGGTGATCGTCACCGCCGGC
GTACCGCGCAAGCCCGGCATGAGCCGCGACGACCTCCTCAGCATCAATCTGAAGGTGATGGAGCAGGTCGGCGCCGGCAT
CAAGAAATACGCCCCCGACGCGTTCGTCATCTGCATCACCAACCCGCTCGACGCGATGGTCTGGGCGCTGCAGAAGGCCT
CCGGCATGCCGGCCAAGAAGGTGGTCGGCATGGCGGGCGTGCTGGACTCGGCGCGCTTCCGTTACTTCCTGGCCGACGAA
TTCAACGTTTCGGTCGAGGACGTCACCGCCTTCGTGCTCGGCGGCCACGGCGACACCATGGTGCCGCTGGTGAAGTACTC
GACGGTGGCGGGCATTCCGCTGCCGGACCTGGTGAAGATGGGCTGGACCTCGCAGGCTCGCCTCGACGAGATCGTCGACC
GCACCCGCAACGGCGGCGCTGAGATCGTCAACCTGCTCAAGACCGGCTCGGCGTTCTACGCGCCGGCCGCCTCGGCGATC
GCGATGGCCGAGAGCTATCTGCGCGACAAGAAGCGCGTGCTGCCGGTCGCCGCCTATCTCAACGGCGAATACGGCGTCAA
GGACATGTATGTCGGCGTGCCGGTGGTGATCGGCGCCAAGGGCGTCGAGCGCATCGTCGAGATCGAAATGGCCGGCAAGG
ATCGCGAAGCGTTCGACAAATCGGTCGGTGCGGTCCAGGGTCTGGTCGACGCCTGCAAGAAGATCGCGCCCGATCTGCTC
GGACGCTAA

Upstream 100 bases:

>100_bases
ATTGGGCGCTGCCCGACTTGAACGCGTCCGGCGAAAGGGATAACCACCCAAGCGGATTTCCCACCCTCTCCCTCCTTCGG
GCTCAAAGGACTCCTTTCCC

Downstream 100 bases:

>100_bases
ATCAGTGTAAGCCTGCCGTGGTAAGCTCCGCCCGGCGAGGGCGCGCCACGGCGGTTCAACCGGCTGGACGCGTTCCGGCG
AGGGGCAAGCGATGAATATC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MARDKIALIGSGQIGGTLAHMIGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDANFTGANSYEAIEGASVVIVTAG
VPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGMPAKKVVGMAGVLDSARFRYFLADE
FNVSVEDVTAFVLGGHGDTMVPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
AMAESYLRDKKRVLPVAAYLNGEYGVKDMYVGVPVVIGAKGVERIVEIEMAGKDREAFDKSVGAVQGLVDACKKIAPDLL
GR

Sequences:

>Translated_322_residues
MARDKIALIGSGQIGGTLAHMIGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDANFTGANSYEAIEGASVVIVTAG
VPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGMPAKKVVGMAGVLDSARFRYFLADE
FNVSVEDVTAFVLGGHGDTMVPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
AMAESYLRDKKRVLPVAAYLNGEYGVKDMYVGVPVVIGAKGVERIVEIEMAGKDREAFDKSVGAVQGLVDACKKIAPDLL
GR
>Mature_321_residues
ARDKIALIGSGQIGGTLAHMIGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDANFTGANSYEAIEGASVVIVTAGV
PRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICITNPLDAMVWALQKASGMPAKKVVGMAGVLDSARFRYFLADEF
NVSVEDVTAFVLGGHGDTMVPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAIA
MAESYLRDKKRVLPVAAYLNGEYGVKDMYVGVPVVIGAKGVERIVEIEMAGKDREAFDKSVGAVQGLVDACKKIAPDLLG
R

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=30.1639344262295, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=30.1639344262295, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI9257228, Length=304, Percent_Identity=30.9210526315789, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI4504973, Length=304, Percent_Identity=30.9210526315789, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=26.4150943396226, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI5031857, Length=319, Percent_Identity=30.0940438871473, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI260099723, Length=319, Percent_Identity=30.0940438871473, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=28.7128712871287, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=28.7128712871287, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI260099727, Length=207, Percent_Identity=33.8164251207729, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI260099725, Length=207, Percent_Identity=33.8164251207729, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=29.2307692307692, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI21735621, Length=298, Percent_Identity=27.1812080536913, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI103472011, Length=319, Percent_Identity=21.6300940438871, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1789632, Length=321, Percent_Identity=30.8411214953271, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=246, Percent_Identity=26.8292682926829, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=311, Percent_Identity=29.903536977492, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=302, Percent_Identity=28.8079470198675, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=242, Percent_Identity=28.9256198347107, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324446, Length=254, Percent_Identity=27.5590551181102, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=28.3828382838284, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550422, Length=299, Percent_Identity=25.4180602006689, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24663595, Length=282, Percent_Identity=30.1418439716312, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647881, Length=300, Percent_Identity=29, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=26.6233766233766, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33980; Mature: 33849

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDKIALIGSGQIGGTLAHMIGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDANF
CCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEHHHCCCCCCCEEECCCCCCCCCCCCCC
TGANSYEAIEGASVVIVTAGVPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICIT
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKASGMPAKKVVGMAGVLDSARFRYFLADEFNVSVEDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEECCCCCCE
VPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
EEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCHHHHH
AMAESYLRDKKRVLPVAAYLNGEYGVKDMYVGVPVVIGAKGVERIVEIEMAGKDREAFDK
HHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCEEEECCHHHHHHEEEEECCCCHHHHHH
SVGAVQGLVDACKKIAPDLLGR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARDKIALIGSGQIGGTLAHMIGLKELGDVVLFDIAEGVPQGKALDIAESSPVDGFDANF
CCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEHHHCCCCCCCEEECCCCCCCCCCCCCC
TGANSYEAIEGASVVIVTAGVPRKPGMSRDDLLSINLKVMEQVGAGIKKYAPDAFVICIT
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKASGMPAKKVVGMAGVLDSARFRYFLADEFNVSVEDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEECCCCCCE
VPLVKYSTVAGIPLPDLVKMGWTSQARLDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
EEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCHHHHH
AMAESYLRDKKRVLPVAAYLNGEYGVKDMYVGVPVVIGAKGVERIVEIEMAGKDREAFDK
HHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCEEEECCHHHHHHEEEEECCCCHHHHHH
SVGAVQGLVDACKKIAPDLLGR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA