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The map label for this gene is ptsP [H]

Identifier: 86747203

GI number: 86747203

Start: 77432

End: 79699

Strand: Reverse

Name: ptsP [H]

Synonym: RPB_0076

Alternate gene names: 86747203

Gene position: 79699-77432 (Counterclockwise)

Preceding gene: 86747204

Following gene: 162138527

Centisome position: 1.49

GC content: 68.03

Gene sequence:

>2268_bases
ATGCGGAGCACGTCGGGCGGCCCCCGCGTCTTGCTGAGACGGCTCCGTGAAACCATGGCGGAGCAAGTCTCCGCGCAGGA
ACGCCTGGACAAGATCGTGGTGCTGATCGCCGCCAATATGGTGGCGGAGGTGTGCTCGACCTACGTCCTGCGGGTCGACA
ATACGCTCGAACTCTACGCCACCGAAGGCCTCAACCGCGAGGCGGTGCACCAGACCGTGCTGACCGCGCATGAAGGCCTG
GTCGGCCTCGTCGCCTCCGAGGCGACGCCGCTGAATCTCTCCGACGCGCAGAGCCATCCGGCGTTCTCGTTCCGCCCGGA
GACCGGCGAAGAAATCTATCACTCCTTCCTCGGCGTGCCGATTCTGCGCGCCGGCAACACGCTCGGCGTGCTGGTGTTGC
AGAACCGCGCCAAGCGCACCTATGTCGAGGAAGAGGTCGAGGCGCTGCAGACCACCGCGATGGTGCTCGCCGAAATGATC
GCCTCGGGCGAATTGTCGGCGCTGGCGCAGCCCGGCGCCGAGCCCGCGGCACGGCACTCGATCCACAAGACCGGCGCGAT
CCTGTCCGACGGCATCGCGCTCGGCCATGTCGTGCTGCACGAGCCGCGCGTCGTCATCACCAACTACATCGCCGAGGATC
TGCCGAAGGAAATCAGGCGGCTCGACACCGCTTTGGCCAATCTGCGCGCCGATCTCGACCGTCTGCTGGAGCGCGGCGAC
GTCGCCGACGGCGGCGAGCATCGCGACGTGCTCGAAGCCTACCGGATGTTCGCCAACGACCACGGCTGGTCGCACAAGCT
GCACGAGGCGGTGGCCACCGGCCTCACCGCGGAAGCCGCCGTCGAGCGCGTGCAGTCCGACACACGGGCCCGGATGCTGC
GCTCGACCGATCCGTATTTGCGCGACCGGCTGCACGATCTCGAGGACCTCGGCCACCGGCTGATGCGCCAGCTGGTCGGC
CAGAATCACGCACCGTCCCGCGAGCAATTGCCCGACAACGCCATCCTGATCGCGCGCTCGATGGGCCCGGCGGCGCTGCT
CGACTACGACCGCAAGAGGCTGCGCGGCCTGGTGCTCGAAGAGGGCACCGCGAATTCGCACGTCTCGATCGTGGCGCGCG
CGCTCGGCATTGCGGCGATCGGCGAAGTGCCGAACGCGCCGGGCATCGCCGATCCGGGCGACGCGATCATCGTCGATGCC
ACCTCCGGCTCGATCTATGTCCGCCCCTCCGCGGAGGTCGAGGCCGCCTATGCCGAGCGGGTGCGCTTTCGCGCGCGGCG
CCAGGCGCAGTATTCCGCGCTGCGCGATCTCGCCTGCGTCACCACCGACGGCCAGCCGATCGACCTGATGATCAATGCCG
GTCTGGCGATCGACCTGCCGCATATCGACGACACCGGCTCGTCCGGCGTCGGCCTGTTCCGCACCGAGCTGCAGTTCATG
ATCGGCCAGAGCCTGCCGCGCTCCAGCGATCAGCTCGCGCTGTACCGCGCCGTGCTCGATGCCGCGGGATCCAAGCCGGT
GACCTTCCGCACGCTCGATATCGGCGGCGACAAGGCGCTGCCCTATATGGAAACCGTGGTCGAGGAGAATCCTGCGCTCG
GCTGGCGGGCGATCCGGCTCGGGCTGGACCGGCCGGGCCTGCTGCGCAGCCAGATCCGGGCGCTGTTGCGCGCCGGCGGC
GGGCGCAATCTGCGCATCATGTTTCCGATGATCTCGGAAGTCGCCGAATTCGACGCCGCCAAGGCGATCGTCGAGCGCGA
ACTGACCTATCTGCGCCAGCACGGCCACACGCTGCCGGAGCGGGTCGACGTCGGCACCATGGTCGAAGTGCCGGCGCTGC
TGTATCAGCTCGACGAACTGTTCAAGAAGGTCGACTTCATTTCGGTCGGCTCCAACGATCTGTTCCAGTTTTTGTATGCG
GTCGACCGCGGCAACGCCAAGGTGTCGGACCGCTTCGACACACTGTCGGCCCCCATCCTGCGGGCGCTGCGCGACATCGT
GCGCAAGGCCAAGGCCGCCAAGCGCTCGGTGTCGCTGTGCGGCGAGATGGCGTCGCAGCCGCTGGGGGCGCTGGCGCTGA
TCGCGATCGGCTATCGGGCGCTGTCGGTGTCGGCGGTGTCGCACGGCCCGGTCAAGGCGATGATCCTCGAACTCGACGCT
GCCAAGGCCGAGGCGGCGATCTCGCCGTTGCTCGATGCGCCCGCCGGCAGCGTCTCGATCCGGCACAAGCTCGCGGAATT
CGCCGAGGCTCACGGGCTGTCCTTGTAG

Upstream 100 bases:

>100_bases
GAGGCCGGGCGGATTGTCGTCCGGCGCGATCGCGTCGAGAGGTTTGTCGCCGGCACCGCAGGCCGGCGGCTATGACGATT
TTCAGAGGAGATGTCTTCAC

Downstream 100 bases:

>100_bases
CGACGCTGTTGTCCGCGCCGCCGCGTCGCCACCATCCTTGCCATTCTCGCATTTCGTTGTCCGAGACCGATCGATGTCCA
ACCTGCCCGAAGCCAAGCTC

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MRSTSGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEVCSTYVLRVDNTLELYATEGLNREAVHQTVLTAHEGL
VGLVASEATPLNLSDAQSHPAFSFRPETGEEIYHSFLGVPILRAGNTLGVLVLQNRAKRTYVEEEVEALQTTAMVLAEMI
ASGELSALAQPGAEPAARHSIHKTGAILSDGIALGHVVLHEPRVVITNYIAEDLPKEIRRLDTALANLRADLDRLLERGD
VADGGEHRDVLEAYRMFANDHGWSHKLHEAVATGLTAEAAVERVQSDTRARMLRSTDPYLRDRLHDLEDLGHRLMRQLVG
QNHAPSREQLPDNAILIARSMGPAALLDYDRKRLRGLVLEEGTANSHVSIVARALGIAAIGEVPNAPGIADPGDAIIVDA
TSGSIYVRPSAEVEAAYAERVRFRARRQAQYSALRDLACVTTDGQPIDLMINAGLAIDLPHIDDTGSSGVGLFRTELQFM
IGQSLPRSSDQLALYRAVLDAAGSKPVTFRTLDIGGDKALPYMETVVEENPALGWRAIRLGLDRPGLLRSQIRALLRAGG
GRNLRIMFPMISEVAEFDAAKAIVERELTYLRQHGHTLPERVDVGTMVEVPALLYQLDELFKKVDFISVGSNDLFQFLYA
VDRGNAKVSDRFDTLSAPILRALRDIVRKAKAAKRSVSLCGEMASQPLGALALIAIGYRALSVSAVSHGPVKAMILELDA
AKAEAAISPLLDAPAGSVSIRHKLAEFAEAHGLSL

Sequences:

>Translated_755_residues
MRSTSGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEVCSTYVLRVDNTLELYATEGLNREAVHQTVLTAHEGL
VGLVASEATPLNLSDAQSHPAFSFRPETGEEIYHSFLGVPILRAGNTLGVLVLQNRAKRTYVEEEVEALQTTAMVLAEMI
ASGELSALAQPGAEPAARHSIHKTGAILSDGIALGHVVLHEPRVVITNYIAEDLPKEIRRLDTALANLRADLDRLLERGD
VADGGEHRDVLEAYRMFANDHGWSHKLHEAVATGLTAEAAVERVQSDTRARMLRSTDPYLRDRLHDLEDLGHRLMRQLVG
QNHAPSREQLPDNAILIARSMGPAALLDYDRKRLRGLVLEEGTANSHVSIVARALGIAAIGEVPNAPGIADPGDAIIVDA
TSGSIYVRPSAEVEAAYAERVRFRARRQAQYSALRDLACVTTDGQPIDLMINAGLAIDLPHIDDTGSSGVGLFRTELQFM
IGQSLPRSSDQLALYRAVLDAAGSKPVTFRTLDIGGDKALPYMETVVEENPALGWRAIRLGLDRPGLLRSQIRALLRAGG
GRNLRIMFPMISEVAEFDAAKAIVERELTYLRQHGHTLPERVDVGTMVEVPALLYQLDELFKKVDFISVGSNDLFQFLYA
VDRGNAKVSDRFDTLSAPILRALRDIVRKAKAAKRSVSLCGEMASQPLGALALIAIGYRALSVSAVSHGPVKAMILELDA
AKAEAAISPLLDAPAGSVSIRHKLAEFAEAHGLSL
>Mature_755_residues
MRSTSGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEVCSTYVLRVDNTLELYATEGLNREAVHQTVLTAHEGL
VGLVASEATPLNLSDAQSHPAFSFRPETGEEIYHSFLGVPILRAGNTLGVLVLQNRAKRTYVEEEVEALQTTAMVLAEMI
ASGELSALAQPGAEPAARHSIHKTGAILSDGIALGHVVLHEPRVVITNYIAEDLPKEIRRLDTALANLRADLDRLLERGD
VADGGEHRDVLEAYRMFANDHGWSHKLHEAVATGLTAEAAVERVQSDTRARMLRSTDPYLRDRLHDLEDLGHRLMRQLVG
QNHAPSREQLPDNAILIARSMGPAALLDYDRKRLRGLVLEEGTANSHVSIVARALGIAAIGEVPNAPGIADPGDAIIVDA
TSGSIYVRPSAEVEAAYAERVRFRARRQAQYSALRDLACVTTDGQPIDLMINAGLAIDLPHIDDTGSSGVGLFRTELQFM
IGQSLPRSSDQLALYRAVLDAAGSKPVTFRTLDIGGDKALPYMETVVEENPALGWRAIRLGLDRPGLLRSQIRALLRAGG
GRNLRIMFPMISEVAEFDAAKAIVERELTYLRQHGHTLPERVDVGTMVEVPALLYQLDELFKKVDFISVGSNDLFQFLYA
VDRGNAKVSDRFDTLSAPILRALRDIVRKAKAAKRSVSLCGEMASQPLGALALIAIGYRALSVSAVSHGPVKAMILELDA
AKAEAAISPLLDAPAGSVSIRHKLAEFAEAHGLSL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=753, Percent_Identity=35.4581673306773, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1788756, Length=539, Percent_Identity=34.6938775510204, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1788726, Length=539, Percent_Identity=31.9109461966605, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI48994992, Length=499, Percent_Identity=32.2645290581162, Blast_Score=258, Evalue=9e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 81967; Mature: 81967

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTSGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEVCSTYVLRVDNTLELYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEE
TEGLNREAVHQTVLTAHEGLVGLVASEATPLNLSDAQSHPAFSFRPETGEEIYHSFLGVP
ECCCCHHHHHHHHHHHHCCHHEEHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCH
ILRAGNTLGVLVLQNRAKRTYVEEEVEALQTTAMVLAEMIASGELSALAQPGAEPAARHS
HEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHH
IHKTGAILSDGIALGHVVLHEPRVVITNYIAEDLPKEIRRLDTALANLRADLDRLLERGD
HHHHCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VADGGEHRDVLEAYRMFANDHGWSHKLHEAVATGLTAEAAVERVQSDTRARMLRSTDPYL
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHH
RDRLHDLEDLGHRLMRQLVGQNHAPSREQLPDNAILIARSMGPAALLDYDRKRLRGLVLE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHEE
EGTANSHVSIVARALGIAAIGEVPNAPGIADPGDAIIVDATSGSIYVRPSAEVEAAYAER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHH
VRFRARRQAQYSALRDLACVTTDGQPIDLMINAGLAIDLPHIDDTGSSGVGLFRTELQFM
HHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHH
IGQSLPRSSDQLALYRAVLDAAGSKPVTFRTLDIGGDKALPYMETVVEENPALGWRAIRL
HHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEE
GLDRPGLLRSQIRALLRAGGGRNLRIMFPMISEVAEFDAAKAIVERELTYLRQHGHTLPE
CCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
RVDVGTMVEVPALLYQLDELFKKVDFISVGSNDLFQFLYAVDRGNAKVSDRFDTLSAPIL
HCCCCHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RALRDIVRKAKAAKRSVSLCGEMASQPLGALALIAIGYRALSVSAVSHGPVKAMILELDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHH
AKAEAAISPLLDAPAGSVSIRHKLAEFAEAHGLSL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSTSGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEVCSTYVLRVDNTLELYA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEE
TEGLNREAVHQTVLTAHEGLVGLVASEATPLNLSDAQSHPAFSFRPETGEEIYHSFLGVP
ECCCCHHHHHHHHHHHHCCHHEEHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCH
ILRAGNTLGVLVLQNRAKRTYVEEEVEALQTTAMVLAEMIASGELSALAQPGAEPAARHS
HEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHH
IHKTGAILSDGIALGHVVLHEPRVVITNYIAEDLPKEIRRLDTALANLRADLDRLLERGD
HHHHCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VADGGEHRDVLEAYRMFANDHGWSHKLHEAVATGLTAEAAVERVQSDTRARMLRSTDPYL
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHH
RDRLHDLEDLGHRLMRQLVGQNHAPSREQLPDNAILIARSMGPAALLDYDRKRLRGLVLE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHEE
EGTANSHVSIVARALGIAAIGEVPNAPGIADPGDAIIVDATSGSIYVRPSAEVEAAYAER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHH
VRFRARRQAQYSALRDLACVTTDGQPIDLMINAGLAIDLPHIDDTGSSGVGLFRTELQFM
HHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHH
IGQSLPRSSDQLALYRAVLDAAGSKPVTFRTLDIGGDKALPYMETVVEENPALGWRAIRL
HHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEE
GLDRPGLLRSQIRALLRAGGGRNLRIMFPMISEVAEFDAAKAIVERELTYLRQHGHTLPE
CCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
RVDVGTMVEVPALLYQLDELFKKVDFISVGSNDLFQFLYAVDRGNAKVSDRFDTLSAPIL
HCCCCHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RALRDIVRKAKAAKRSVSLCGEMASQPLGALALIAIGYRALSVSAVSHGPVKAMILELDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHH
AKAEAAISPLLDAPAGSVSIRHKLAEFAEAHGLSL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]