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The map label for this gene is prfA

Identifier: 162138527

GI number: 162138527

Start: 76273

End: 77358

Strand: Reverse

Name: prfA

Synonym: RPB_0075

Alternate gene names: 162138527

Gene position: 77358-76273 (Counterclockwise)

Preceding gene: 86747203

Following gene: 86747201

Centisome position: 1.45

GC content: 66.11

Gene sequence:

>1086_bases
ATGTCCAACCTGCCCGAAGCCAAGCTCGACGTTCTGCTCGCGCATCATGCCTCGCTCGAAGCGCAATTGCTCGGCGAGGT
CGCGGCCAATGATTACGTCCGCATCACTCGCGAATTGTCCGAGTTGAATCCGCTGGTCGAGGCGGTGAAGGCCTATCGCG
AAGTCCGCGACGAACTCGGCGACATCGACGATCTGCTCGAAGATCCCGCGACCGATCCGGAGATGCGGGCGATGGCGGAG
GCCGAGCGCGACGCGCTCGATGCCCATCGCGAGGACCTGATCCAGCAGATCCGGGTCGCGCTGCTGCCGAAGGACGCGAT
GGACGAGCGCAACGTGATGCTCGAAATCCGCGCCGGCACCGGCGGCGACGAGGCCTCGCTGTTCGCCGGCGACCTGTTCC
GGATGTACGAGAAATTCGCGGCCTTGCAGGGCTGGAGCGTCGAGGTGATCTCGGCCAGCGAGGGCACCGTCGGCGGCTTC
AAGGAGATCATCGCCGAGGTGAAGGGGCGTGGCGCCTTCGCCAAGCTGAAATTCGAATCCGGCGTGCATCGGGTGCAGCG
CGTGCCCGACACCGAGACGCAGGGGCGCATCCACACCTCGGCGGCGACCGTGGCGGTGCTGCCCGAGGTCGAGGAGGTCG
ACGTCGACATCAAGCCGGATGATCTCAAGATCGACACGATGCGGGCACAGGGCGCCGGCGGCCAGCACGTCAACAAGACC
GAATCGGCGATCCGCATCACTCATCTGCCGACCGGCATCGTGGTGATGATGCAGGACAGCCGCTCGCAGCACAAAAACCG
CGCCTCGGCGATGAACATCCTGCGTTCGCGAATCTACGACGCCGAGCAGCAGCGGATCGATTCGGCGCGCTCGGCCGAGC
GCAAGCAGAAGGTCGGCTCCGGCGACCGCTCGGAGCGGATCCGCACCTACAACTTCCCGCAGGGCCGCGTCACCGATCAC
CGCATCAATCTGACGCTGTACAAGCTGCCGCAGGTGATCGCCGGCGAAGCGCTCGGCGAGCTGATCGACGCGCTGACCAC
CGAACACCAGGTGGCCCAGCTCGCGGCGCAGGGCCATGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGAGGCTCACGGGCTGTCCTTGTAGCGACGCTGTTGTCCGCGCCGCCGCGTCGCCACCATCCTTGCCATTCTCGCATT
TCGTTGTCCGAGACCGATCG

Downstream 100 bases:

>100_bases
GCCGCACAAAAGCGAATTTACAATCGAACTAATGTTGTGCCTCATTATCCTTTTCTAGGGCGACTCAACCATGGCAACCG
AAATTTCTGATTTTATTTGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSNLPEAKLDVLLAHHASLEAQLLGEVAANDYVRITRELSELNPLVEAVKAYREVRDELGDIDDLLEDPATDPEMRAMAE
AERDALDAHREDLIQQIRVALLPKDAMDERNVMLEIRAGTGGDEASLFAGDLFRMYEKFAALQGWSVEVISASEGTVGGF
KEIIAEVKGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEVEEVDVDIKPDDLKIDTMRAQGAGGQHVNKT
ESAIRITHLPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAEQQRIDSARSAERKQKVGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLPQVIAGEALGELIDALTTEHQVAQLAAQGHAA

Sequences:

>Translated_361_residues
MSNLPEAKLDVLLAHHASLEAQLLGEVAANDYVRITRELSELNPLVEAVKAYREVRDELGDIDDLLEDPATDPEMRAMAE
AERDALDAHREDLIQQIRVALLPKDAMDERNVMLEIRAGTGGDEASLFAGDLFRMYEKFAALQGWSVEVISASEGTVGGF
KEIIAEVKGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEVEEVDVDIKPDDLKIDTMRAQGAGGQHVNKT
ESAIRITHLPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAEQQRIDSARSAERKQKVGSGDRSERIRTYNFPQGRVTDH
RINLTLYKLPQVIAGEALGELIDALTTEHQVAQLAAQGHAA
>Mature_360_residues
SNLPEAKLDVLLAHHASLEAQLLGEVAANDYVRITRELSELNPLVEAVKAYREVRDELGDIDDLLEDPATDPEMRAMAEA
ERDALDAHREDLIQQIRVALLPKDAMDERNVMLEIRAGTGGDEASLFAGDLFRMYEKFAALQGWSVEVISASEGTVGGFK
EIIAEVKGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEVEEVDVDIKPDDLKIDTMRAQGAGGQHVNKTE
SAIRITHLPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAEQQRIDSARSAERKQKVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLPQVIAGEALGELIDALTTEHQVAQLAAQGHAA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=46.0264900662252, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=37.125748502994, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=45.4106280193237, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=54.9295774647887, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI2367172, Length=315, Percent_Identity=37.4603174603175, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=300, Percent_Identity=40.3333333333333, Blast_Score=214, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321295, Length=319, Percent_Identity=42.0062695924765, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19921226, Length=323, Percent_Identity=41.4860681114551, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_RHOP2 (Q2J423)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483698.2
- ProteinModelPortal:   Q2J423
- SMR:   Q2J423
- STRING:   Q2J423
- GeneID:   3907815
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0075
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- ProtClustDB:   PRK00591
- BioCyc:   RPAL316058:RPB_0075-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39826; Mature: 39695

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLPEAKLDVLLAHHASLEAQLLGEVAANDYVRITRELSELNPLVEAVKAYREVRDELG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DIDDLLEDPATDPEMRAMAEAERDALDAHREDLIQQIRVALLPKDAMDERNVMLEIRAGT
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEECCC
GGDEASLFAGDLFRMYEKFAALQGWSVEVISASEGTVGGFKEIIAEVKGRGAFAKLKFES
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHH
GVHRVQRVPDTETQGRIHTSAATVAVLPEVEEVDVDIKPDDLKIDTMRAQGAGGQHVNKT
HHHHHHCCCCCCCCCEEEECCEEEEECCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
ESAIRITHLPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAEQQRIDSARSAERKQKVGS
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLPQVIAGEALGELIDALTTEHQVAQLAAQGHA
CCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
A
C
>Mature Secondary Structure 
SNLPEAKLDVLLAHHASLEAQLLGEVAANDYVRITRELSELNPLVEAVKAYREVRDELG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DIDDLLEDPATDPEMRAMAEAERDALDAHREDLIQQIRVALLPKDAMDERNVMLEIRAGT
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEECCC
GGDEASLFAGDLFRMYEKFAALQGWSVEVISASEGTVGGFKEIIAEVKGRGAFAKLKFES
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHH
GVHRVQRVPDTETQGRIHTSAATVAVLPEVEEVDVDIKPDDLKIDTMRAQGAGGQHVNKT
HHHHHHCCCCCCCCCEEEECCEEEEECCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCC
ESAIRITHLPTGIVVMMQDSRSQHKNRASAMNILRSRIYDAEQQRIDSARSAERKQKVGS
CCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFPQGRVTDHRINLTLYKLPQVIAGEALGELIDALTTEHQVAQLAAQGHA
CCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA