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The map label for this gene is sppA-2 [C]

Identifier: 86608463

GI number: 86608463

Start: 1019366

End: 1020172

Strand: Reverse

Name: sppA-2 [C]

Synonym: CYB_0984

Alternate gene names: 86608463

Gene position: 1020172-1019366 (Counterclockwise)

Preceding gene: 86608464

Following gene: 86608461

Centisome position: 33.48

GC content: 55.27

Gene sequence:

>807_bases
ATGCTGAAGCGATTATTCTTGGGTGGTTTGCGCCGGCAAATCGGTCGCATTGAGGTGGCAGGGGCCATTGATGGCAAAAC
TCGCGAGCGGGTTCTCAAGGCCCTGAAAGAGGTGGAAGAAAACCAGATCCCTGCCGTGGTGCTGCGCATCGACAGTCCAG
GCGGGACGGTGGCCGACTCCCAAGAAATCTACAATGCCCTCTTGCGTTTGCGAGAGAAAAAGGGCACCCGTATTGTCGCC
AGCTTTGGCAACATTGCTGCCTCTGGGGGGGTTTATATTGCCATGGGAGCCGAAAAAATTGTTGCCAACCCAGGAACAAT
CACCGGTAGCATTGGCGTGATCATTCGAGGCAACAATATCGAACGCCTCCTGGATAAGGTGGGGATTTCATTTAAGGTGA
TTAAGTCTGGCCCCTACAAAGATATTCTCTCGTTTGATCGCGAGCTGACCCCAGAAGAGCGAAACATCCTGCAAGAGTTG
ATCGACATCACCTACGGCCAGTTTGTGCAGACGGTTGCTGCTGCCCGCAAGTTGAGTTTGAATGTGGTCAAAACTTTTGC
CGATGGCCGCATTTTCAGCGGAGAGCAGGCCCTTCACTTGGGTGTGGTGGATCGCTTGGGCACAGAGGAAGATGCCCGCC
TTTGGGCGGCAGAGCTGGCGGGTTTGCCGACGGATGCGAAGTTCTACGACCTGCTCCCGCGGCGCAGAGGTTGGTCTCGG
TTTTTGCCGGCAGCAGAAGCCTTGGCCCGTCTGCAGTTTGAAGTGAGCACCTCGGGCTTGCCCCTGTGGCTTTACACAGG
TTCTTGA

Upstream 100 bases:

>100_bases
TGATCCCGGTGTATGGCAACGTCTAGGCAACTGGTTGACCAAGCCATGAGCCGCCATTCATGATACAGAATATGCACTTA
TCCCTATCCAGTTCTTTTCC

Downstream 100 bases:

>100_bases
GGCAGTTCGCGAACAGCTATTCCGGGAAGTTTAGCGTTCTGTACCGCACAGCCCCCACCCCCGGCCCCTCTTCCAGAGGG
AGAGGGAGTTTAACTAAAGT

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLKRLFLGGLRRQIGRIEVAGAIDGKTRERVLKALKEVEENQIPAVVLRIDSPGGTVADSQEIYNALLRLREKKGTRIVA
SFGNIAASGGVYIAMGAEKIVANPGTITGSIGVIIRGNNIERLLDKVGISFKVIKSGPYKDILSFDRELTPEERNILQEL
IDITYGQFVQTVAAARKLSLNVVKTFADGRIFSGEQALHLGVVDRLGTEEDARLWAAELAGLPTDAKFYDLLPRRRGWSR
FLPAAEALARLQFEVSTSGLPLWLYTGS

Sequences:

>Translated_268_residues
MLKRLFLGGLRRQIGRIEVAGAIDGKTRERVLKALKEVEENQIPAVVLRIDSPGGTVADSQEIYNALLRLREKKGTRIVA
SFGNIAASGGVYIAMGAEKIVANPGTITGSIGVIIRGNNIERLLDKVGISFKVIKSGPYKDILSFDRELTPEERNILQEL
IDITYGQFVQTVAAARKLSLNVVKTFADGRIFSGEQALHLGVVDRLGTEEDARLWAAELAGLPTDAKFYDLLPRRRGWSR
FLPAAEALARLQFEVSTSGLPLWLYTGS
>Mature_268_residues
MLKRLFLGGLRRQIGRIEVAGAIDGKTRERVLKALKEVEENQIPAVVLRIDSPGGTVADSQEIYNALLRLREKKGTRIVA
SFGNIAASGGVYIAMGAEKIVANPGTITGSIGVIIRGNNIERLLDKVGISFKVIKSGPYKDILSFDRELTPEERNILQEL
IDITYGQFVQTVAAARKLSLNVVKTFADGRIFSGEQALHLGVVDRLGTEEDARLWAAELAGLPTDAKFYDLLPRRRGWSR
FLPAAEALARLQFEVSTSGLPLWLYTGS

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=179, Percent_Identity=39.1061452513966, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787527, Length=166, Percent_Identity=35.5421686746988, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 29376; Mature: 29376

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLFLGGLRRQIGRIEVAGAIDGKTRERVLKALKEVEENQIPAVVLRIDSPGGTVADS
CCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCH
QEIYNALLRLREKKGTRIVASFGNIAASGGVYIAMGAEKIVANPGTITGSIGVIIRGNNI
HHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEECCHHEECCCCEEEEEEEEEEECCCH
ERLLDKVGISFKVIKSGPYKDILSFDRELTPEERNILQELIDITYGQFVQTVAAARKLSL
HHHHHHHCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVVKTFADGRIFSGEQALHLGVVDRLGTEEDARLWAAELAGLPTDAKFYDLLPRRRGWSR
HHHHHHCCCCEECCCCEEEEHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHH
FLPAAEALARLQFEVSTSGLPLWLYTGS
HCHHHHHHHHHEEEEECCCCEEEEEECC
>Mature Secondary Structure
MLKRLFLGGLRRQIGRIEVAGAIDGKTRERVLKALKEVEENQIPAVVLRIDSPGGTVADS
CCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCH
QEIYNALLRLREKKGTRIVASFGNIAASGGVYIAMGAEKIVANPGTITGSIGVIIRGNNI
HHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEECCHHEECCCCEEEEEEEEEEECCCH
ERLLDKVGISFKVIKSGPYKDILSFDRELTPEERNILQELIDITYGQFVQTVAAARKLSL
HHHHHHHCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVVKTFADGRIFSGEQALHLGVVDRLGTEEDARLWAAELAGLPTDAKFYDLLPRRRGWSR
HHHHHHCCCCEECCCCEEEEHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHH
FLPAAEALARLQFEVSTSGLPLWLYTGS
HCHHHHHHHHHEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]