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The map label for this gene is pilT-1 [H]

Identifier: 86607688

GI number: 86607688

Start: 199788

End: 201146

Strand: Reverse

Name: pilT-1 [H]

Synonym: CYB_0187

Alternate gene names: 86607688

Gene position: 201146-199788 (Counterclockwise)

Preceding gene: 86607689

Following gene: 86607687

Centisome position: 6.6

GC content: 59.97

Gene sequence:

>1359_bases
GTGCTGGGTTCAGCTTCCCAAGGGATGGGATCCCGGCCTGTCAACGCCAAAAGCCCCCCCAACCCCATTCTGGGTCGGCC
CCTTCCTCCTGGTGTGGCTGTCGCTCCCCCTCAGGTGCCTGTGCCTGTGCCCGATCCTGTCGCTCCCCCTCGAATGCCCC
CGCCCACTCCGGCTCCGGCTGCTGCTGCTCGGCCTCCAGTGTCGCCACCTGTCCCCACTCCTGCTGCCCCGCCTTTTGCG
CCACCAGCTCGCCCAACGGCTGCCGGTCGGATCCCAGTGGCTCCAGCTGCTCCTGCGGGCCTGACCCTGCGCCAACTGGT
GCAGGAAGCCTATGACAAAGGCTACTCAGATATTCATTTGGGGGTAGGAGAAGTGCCCCGCTTTCGGGATCGGGGGCAAA
TGCTTTTGACCAACTACCCGGTCACCACCACCGAGATGTTCTATGGCTGGCTGCGGGAAAGCCTGCCGGAGAGCCAAATT
CGCCAGTTTGAGGAATGTTTGGAACTGGATACTGCCATCCAGTACGAGGGTTTGGTGCGGTGTCGGGTCAACTGCTTTGT
TTCCCTTCTTGGCCCGGCTATGGTGATGCGTCTGATCCCGTTGAAAATTTTGACGCTGGAGCAGTTGCGGGCGCCGGAAG
TCTTGAAGCGCATTGCCGAAGAGCACAAAGGCTTGGTTTTGGTGACCGGCCCCACCGGATCCGGTAAGTCCACAACCTTG
GCAGCGATGGTGGACTACATCAATTCCAACTTTCCCAAACACATCATTTCCATTGAGGATCCCATCGAGTTCGTCCACCA
GAGCAAGAAATCGATGGTGCGGCAGCGGGAGGTCGGGATCCACACCCTGGAATTTGCCAATGCCCTGAAAGCCTCTCTGC
GGGAAGATCCGGACATCATCCTGATTGGGGAGATGCGGGATCGAGAGACAGTGGATATTGCCCTGAAGGCCGGCCAGACG
GGCCACTTGGTCTTTGGAACCCTGCACACCAACAGCGCCGTCCGCACCATCGAGCGTCTGCTGGGGATTTACAAGCCGGA
AGAACAGGAGCCGATGCGTCTGCAGCTGGCAGAAACCCTGGTGGCGATCATCGCTCAAACCCTGGTGCGCACCACCGATG
GCAAGCGAGCTGCGGCCCACGAGATCATGCGCAACACCGATGCCATTCGGGACTACATCAAGCGGGGGGCGGTGGACGAA
ATTGAGGAGCTGATCGCCGACGGCTCCTATGAGGGCATGTGTACCATGAATCAATCCCTGTTTAGGCTTTACCAAGAGGG
GCGGATTACCGAAGAGGTGTGTCTGGAGGCTGCCCCACGCCGCAATGAGATGGCCCAATGGCTGCGAGGCCGGATCTAG

Upstream 100 bases:

>100_bases
GGACTCCAATTGGCAGCCCCCGGCTCCGCCGGTCATGCAGCGTCCGCCTCGTCCGGCTGCTCAAACGCCCCCGGTGGAGC
CCCCAACGCAACTGGATCCG

Downstream 100 bases:

>100_bases
GGCCAGTCTCGAGATGGAGTCACGCCAAAGTGCTTGAGGGAAGGTTTGCCCTATCCTAAACTGGGAGCAGCCGGCGGCGC
TGGCTTGCCGAGCTACCTGC

Product: twitching mobility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLGSASQGMGSRPVNAKSPPNPILGRPLPPGVAVAPPQVPVPVPDPVAPPRMPPPTPAPAAAARPPVSPPVPTPAAPPFA
PPARPTAAGRIPVAPAAPAGLTLRQLVQEAYDKGYSDIHLGVGEVPRFRDRGQMLLTNYPVTTTEMFYGWLRESLPESQI
RQFEECLELDTAIQYEGLVRCRVNCFVSLLGPAMVMRLIPLKILTLEQLRAPEVLKRIAEEHKGLVLVTGPTGSGKSTTL
AAMVDYINSNFPKHIISIEDPIEFVHQSKKSMVRQREVGIHTLEFANALKASLREDPDIILIGEMRDRETVDIALKAGQT
GHLVFGTLHTNSAVRTIERLLGIYKPEEQEPMRLQLAETLVAIIAQTLVRTTDGKRAAAHEIMRNTDAIRDYIKRGAVDE
IEELIADGSYEGMCTMNQSLFRLYQEGRITEEVCLEAAPRRNEMAQWLRGRI

Sequences:

>Translated_452_residues
MLGSASQGMGSRPVNAKSPPNPILGRPLPPGVAVAPPQVPVPVPDPVAPPRMPPPTPAPAAAARPPVSPPVPTPAAPPFA
PPARPTAAGRIPVAPAAPAGLTLRQLVQEAYDKGYSDIHLGVGEVPRFRDRGQMLLTNYPVTTTEMFYGWLRESLPESQI
RQFEECLELDTAIQYEGLVRCRVNCFVSLLGPAMVMRLIPLKILTLEQLRAPEVLKRIAEEHKGLVLVTGPTGSGKSTTL
AAMVDYINSNFPKHIISIEDPIEFVHQSKKSMVRQREVGIHTLEFANALKASLREDPDIILIGEMRDRETVDIALKAGQT
GHLVFGTLHTNSAVRTIERLLGIYKPEEQEPMRLQLAETLVAIIAQTLVRTTDGKRAAAHEIMRNTDAIRDYIKRGAVDE
IEELIADGSYEGMCTMNQSLFRLYQEGRITEEVCLEAAPRRNEMAQWLRGRI
>Mature_452_residues
MLGSASQGMGSRPVNAKSPPNPILGRPLPPGVAVAPPQVPVPVPDPVAPPRMPPPTPAPAAAARPPVSPPVPTPAAPPFA
PPARPTAAGRIPVAPAAPAGLTLRQLVQEAYDKGYSDIHLGVGEVPRFRDRGQMLLTNYPVTTTEMFYGWLRESLPESQI
RQFEECLELDTAIQYEGLVRCRVNCFVSLLGPAMVMRLIPLKILTLEQLRAPEVLKRIAEEHKGLVLVTGPTGSGKSTTL
AAMVDYINSNFPKHIISIEDPIEFVHQSKKSMVRQREVGIHTLEFANALKASLREDPDIILIGEMRDRETVDIALKAGQT
GHLVFGTLHTNSAVRTIERLLGIYKPEEQEPMRLQLAETLVAIIAQTLVRTTDGKRAAAHEIMRNTDAIRDYIKRGAVDE
IEELIADGSYEGMCTMNQSLFRLYQEGRITEEVCLEAAPRRNEMAQWLRGRI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=39.5136778115502, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789723, Length=201, Percent_Identity=37.8109452736318, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786296, Length=149, Percent_Identity=45.6375838926174, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 49593; Mature: 49593

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGSASQGMGSRPVNAKSPPNPILGRPLPPGVAVAPPQVPVPVPDPVAPPRMPPPTPAPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
AAARPPVSPPVPTPAAPPFAPPARPTAAGRIPVAPAAPAGLTLRQLVQEAYDKGYSDIHL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEC
GVGEVPRFRDRGQMLLTNYPVTTTEMFYGWLRESLPESQIRQFEECLELDTAIQYEGLVR
CCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
CRVNCFVSLLGPAMVMRLIPLKILTLEQLRAPEVLKRIAEEHKGLVLVTGPTGSGKSTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCHHHH
AAMVDYINSNFPKHIISIEDPIEFVHQSKKSMVRQREVGIHTLEFANALKASLREDPDII
HHHHHHHHCCCCHHEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEE
LIGEMRDRETVDIALKAGQTGHLVFGTLHTNSAVRTIERLLGIYKPEEQEPMRLQLAETL
EECCCCCCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VAIIAQTLVRTTDGKRAAAHEIMRNTDAIRDYIKRGAVDEIEELIADGSYEGMCTMNQSL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHHHH
FRLYQEGRITEEVCLEAAPRRNEMAQWLRGRI
HHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLGSASQGMGSRPVNAKSPPNPILGRPLPPGVAVAPPQVPVPVPDPVAPPRMPPPTPAPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
AAARPPVSPPVPTPAAPPFAPPARPTAAGRIPVAPAAPAGLTLRQLVQEAYDKGYSDIHL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEC
GVGEVPRFRDRGQMLLTNYPVTTTEMFYGWLRESLPESQIRQFEECLELDTAIQYEGLVR
CCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
CRVNCFVSLLGPAMVMRLIPLKILTLEQLRAPEVLKRIAEEHKGLVLVTGPTGSGKSTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCHHHH
AAMVDYINSNFPKHIISIEDPIEFVHQSKKSMVRQREVGIHTLEFANALKASLREDPDII
HHHHHHHHCCCCHHEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEE
LIGEMRDRETVDIALKAGQTGHLVFGTLHTNSAVRTIERLLGIYKPEEQEPMRLQLAETL
EECCCCCCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VAIIAQTLVRTTDGKRAAAHEIMRNTDAIRDYIKRGAVDEIEELIADGSYEGMCTMNQSL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHHHHHH
FRLYQEGRITEEVCLEAAPRRNEMAQWLRGRI
HHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]