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The map label for this gene is lepA [H]

Identifier: 86607566

GI number: 86607566

Start: 75214

End: 77025

Strand: Reverse

Name: lepA [H]

Synonym: CYB_0064

Alternate gene names: 86607566

Gene position: 77025-75214 (Counterclockwise)

Preceding gene: 86607567

Following gene: 86607565

Centisome position: 2.53

GC content: 56.79

Gene sequence:

>1812_bases
ATGACCGGCGTTCCTGTCTCCCGTATTCGCAACTTTTCCATCATCGCCCACATCGACCACGGCAAATCCACCCTCGCCGA
TCGCCTCCTGCAGGAAACGGGCACCGTTTCAGCCCGCGAGATGAGGCCACAACTGCTGGACAGCATGGAACTGGAGCGGG
AACGGGGCATTACCATCAAGCTGCAAGCGGCCCGCATGAACTACCGTGCCCAAGACGGCCAAGACTATGTTTTGAACCTG
ATCGACACGCCGGGGCATGTGGACTTCACCTACGAAGTCTCCCGCTCCCTGGCCGCCTGTGAAGGGGCGCTGCTGGTGGT
GGATGCCTCTCAAGGGGTGGAGGCCCAAACCCTGGCCAACGTCTACCTGGCCCTGGAGAACAACCTGGAAATTATTCCGG
TGATCAACAAAATCGACCTGCCGGGCGCCGAGCCGGAGCGGGTCATTGCCGAGATTGAGCAGGTAATCGGCCTCGACTGC
AGCGGGGCAATCCTCGCCTCTGCCAAGGTGGGTATTGGCATTCAAGAGATTCTGGAGGCCGTTGTCCAGCGGGTGCCGCC
CCCACGGGATACGGTGGCGGAGCCCCTGCGGGCCTTGATTTTCGATAGCTACTACGACGCCTATCGCGGCGTGATCGTCT
ATGTGCGCATCATGGATGGGGAGGTGAAGACGGGGGACAAGATCTGCTTTATGGCCTCTGGCCGAGAATACGAGATTACC
GAGCTGGGGGTGATGCGTCCCAACCAAGAGCCGGTGGATGCCCTACATGCGGGCGAGGTGGGCTACATGGCCGCCGCCAT
CAAGAGCGTGGAACACGCCCGTGTTGGGGATACCATCACACTGGTGAATCGCAAAGCTCCTGAGCCCCTGCCCGGCTACA
AAGAAGCCAAGCCGATGGTGTTTTGTGGCCTGTTCCCCAGCAGCTCCGATCAGTACGCCGAACTCAAAGAGGCTCTGGAA
AAGCTCAAGCTCAACGATGCTGCCCTGTACTTTGAGCCAGAAATTTCCAGCGCCATGGGCTTCGGCTTCCGCTGTGGTTT
TCTGGGCCTGCTGCACATGGAGGTGGTGCAGGAACGCCTAGAGCGGGAGTACAATCTCGATCTGGTGATCACAGCTCCGA
CGGTGGTGTACCAAGTTACCCTCAACAACGGCTCTGTGATTCGGGTGGACAATCCCAGCAAGCTGCCTCCCCCCAACGAG
CGCACCAGCATTGAGGAGCCGATTGTCAGGGTAGAGGTGATTCTTCCTGAGGAATTTGTCGGTACCGTGATGGAGCTGTG
TGAGACCAAACGCGGCACTTTTAAAGACATGAAATATCTGGCGCAGGGTCGGACTACGCTGGTGTATGAGCTGCCCCTGG
CGGAAGTGGTCACCGACTTTTTCGACCAATTGAAATCACGCACCCGCGGTTATGCCAGCATGGACTACCAAATGATTGGC
TATCGCGCCGATGATTTGGTGCGTTTGGACATTTTGATCAACGGCGATGTGGTGGATTCTCTATCCACAATTGTGCATCG
GGACAAAGCTTTTTATGTAGGCCGTTCCCTAGTTGCCAAGTTACGAGAGCTGATCCCACGCCATCAATTTGAAGTGCCCA
TTCAGGCGGCAATTGGCTCTAAGGTGATTGCTCGTGAAACCATACCAGCCCTGCGCAAAAACGTCTTGGCCAAATGCTAC
GGAGGAGATGTGACCCGCAAACGCAAGCTTTTGGAAAAACAAAAAGAGGGGAAAAAACGCATGAAGGCGGTGGGAACGGT
GGATGTGCCCCAAGAAGCGTTTATGGCCGTGCTGAAGCTGCAGAATGACTAG

Upstream 100 bases:

>100_bases
TGTCTTCTCCCCTTGGCAGCCAACTTCTGGGCAACCTCAGTTCAGGCAGGGGAGCAGAGAGGCTAGCATTGTAAAAAATC
TTTGCCCTGATCAGGTTTTT

Downstream 100 bases:

>100_bases
GGCGATGGGCACTCAACTGGGGCGGGAAGAGGTGTTGGCCTGGCTACAGGAGCGGGTGTCTCCTCCTCGCCTCCGCCATA
TCTTGGGGGTAGAGGGGATG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MTGVPVSRIRNFSIIAHIDHGKSTLADRLLQETGTVSAREMRPQLLDSMELERERGITIKLQAARMNYRAQDGQDYVLNL
IDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVINKIDLPGAEPERVIAEIEQVIGLDC
SGAILASAKVGIGIQEILEAVVQRVPPPRDTVAEPLRALIFDSYYDAYRGVIVYVRIMDGEVKTGDKICFMASGREYEIT
ELGVMRPNQEPVDALHAGEVGYMAAAIKSVEHARVGDTITLVNRKAPEPLPGYKEAKPMVFCGLFPSSSDQYAELKEALE
KLKLNDAALYFEPEISSAMGFGFRCGFLGLLHMEVVQERLEREYNLDLVITAPTVVYQVTLNNGSVIRVDNPSKLPPPNE
RTSIEEPIVRVEVILPEEFVGTVMELCETKRGTFKDMKYLAQGRTTLVYELPLAEVVTDFFDQLKSRTRGYASMDYQMIG
YRADDLVRLDILINGDVVDSLSTIVHRDKAFYVGRSLVAKLRELIPRHQFEVPIQAAIGSKVIARETIPALRKNVLAKCY
GGDVTRKRKLLEKQKEGKKRMKAVGTVDVPQEAFMAVLKLQND

Sequences:

>Translated_603_residues
MTGVPVSRIRNFSIIAHIDHGKSTLADRLLQETGTVSAREMRPQLLDSMELERERGITIKLQAARMNYRAQDGQDYVLNL
IDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVINKIDLPGAEPERVIAEIEQVIGLDC
SGAILASAKVGIGIQEILEAVVQRVPPPRDTVAEPLRALIFDSYYDAYRGVIVYVRIMDGEVKTGDKICFMASGREYEIT
ELGVMRPNQEPVDALHAGEVGYMAAAIKSVEHARVGDTITLVNRKAPEPLPGYKEAKPMVFCGLFPSSSDQYAELKEALE
KLKLNDAALYFEPEISSAMGFGFRCGFLGLLHMEVVQERLEREYNLDLVITAPTVVYQVTLNNGSVIRVDNPSKLPPPNE
RTSIEEPIVRVEVILPEEFVGTVMELCETKRGTFKDMKYLAQGRTTLVYELPLAEVVTDFFDQLKSRTRGYASMDYQMIG
YRADDLVRLDILINGDVVDSLSTIVHRDKAFYVGRSLVAKLRELIPRHQFEVPIQAAIGSKVIARETIPALRKNVLAKCY
GGDVTRKRKLLEKQKEGKKRMKAVGTVDVPQEAFMAVLKLQND
>Mature_602_residues
TGVPVSRIRNFSIIAHIDHGKSTLADRLLQETGTVSAREMRPQLLDSMELERERGITIKLQAARMNYRAQDGQDYVLNLI
DTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVINKIDLPGAEPERVIAEIEQVIGLDCS
GAILASAKVGIGIQEILEAVVQRVPPPRDTVAEPLRALIFDSYYDAYRGVIVYVRIMDGEVKTGDKICFMASGREYEITE
LGVMRPNQEPVDALHAGEVGYMAAAIKSVEHARVGDTITLVNRKAPEPLPGYKEAKPMVFCGLFPSSSDQYAELKEALEK
LKLNDAALYFEPEISSAMGFGFRCGFLGLLHMEVVQERLEREYNLDLVITAPTVVYQVTLNNGSVIRVDNPSKLPPPNER
TSIEEPIVRVEVILPEEFVGTVMELCETKRGTFKDMKYLAQGRTTLVYELPLAEVVTDFFDQLKSRTRGYASMDYQMIGY
RADDLVRLDILINGDVVDSLSTIVHRDKAFYVGRSLVAKLRELIPRHQFEVPIQAAIGSKVIARETIPALRKNVLAKCYG
GDVTRKRKLLEKQKEGKKRMKAVGTVDVPQEAFMAVLKLQND

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=610, Percent_Identity=51.1475409836066, Blast_Score=667, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=45.9259259259259, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI18390331, Length=208, Percent_Identity=35.0961538461538, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI25306283, Length=153, Percent_Identity=42.483660130719, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI19923640, Length=153, Percent_Identity=42.483660130719, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI25306287, Length=153, Percent_Identity=42.483660130719, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=40.6896551724138, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI310132016, Length=112, Percent_Identity=42.8571428571429, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI310110807, Length=112, Percent_Identity=42.8571428571429, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI310123363, Length=112, Percent_Identity=42.8571428571429, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.8345864661654, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.8345864661654, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI94966752, Length=89, Percent_Identity=42.6966292134831, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=58.0267558528428, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=478, Percent_Identity=29.9163179916318, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1789738, Length=187, Percent_Identity=36.3636363636364, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=33.1288343558282, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789559, Length=234, Percent_Identity=29.0598290598291, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367247, Length=250, Percent_Identity=28, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789108, Length=148, Percent_Identity=31.0810810810811, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=43.3715220949263, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=41.4965986394558, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=36.1290322580645, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=38.9705882352941, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=38.9705882352941, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=35.4838709677419, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.8345864661654, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=240, Percent_Identity=29.5833333333333, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=45.1986754966887, Blast_Score=548, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=37.9120879120879, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=42.4657534246575, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=42.4657534246575, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6322359, Length=151, Percent_Identity=38.4105960264901, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=157, Percent_Identity=38.8535031847134, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=241, Percent_Identity=27.8008298755187, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322675, Length=149, Percent_Identity=28.8590604026846, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=46.1920529801325, Blast_Score=556, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24582462, Length=187, Percent_Identity=39.0374331550802, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28574573, Length=217, Percent_Identity=34.5622119815668, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=39.3333333333333, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=34.0740740740741, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=25.7396449704142, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=283, Percent_Identity=27.208480565371, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864358, Length=283, Percent_Identity=27.208480565371, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921738, Length=232, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922690, Length=332, Percent_Identity=23.1927710843373, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67075; Mature: 66944

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVPVSRIRNFSIIAHIDHGKSTLADRLLQETGTVSAREMRPQLLDSMELERERGITIK
CCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEE
LQAARMNYRAQDGQDYVLNLIDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLAN
EEEEECCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
VYLALENNLEIIPVINKIDLPGAEPERVIAEIEQVIGLDCSGAILASAKVGIGIQEILEA
EEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
VVQRVPPPRDTVAEPLRALIFDSYYDAYRGVIVYVRIMDGEVKTGDKICFMASGREYEIT
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEE
ELGVMRPNQEPVDALHAGEVGYMAAAIKSVEHARVGDTITLVNRKAPEPLPGYKEAKPMV
EECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEE
FCGLFPSSSDQYAELKEALEKLKLNDAALYFEPEISSAMGFGFRCGFLGLLHMEVVQERL
EEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHH
EREYNLDLVITAPTVVYQVTLNNGSVIRVDNPSKLPPPNERTSIEEPIVRVEVILPEEFV
HHHCCCEEEEECCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHH
GTVMELCETKRGTFKDMKYLAQGRTTLVYELPLAEVVTDFFDQLKSRTRGYASMDYQMIG
HHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEE
YRADDLVRLDILINGDVVDSLSTIVHRDKAFYVGRSLVAKLRELIPRHQFEVPIQAAIGS
ECCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
KVIARETIPALRKNVLAKCYGGDVTRKRKLLEKQKEGKKRMKAVGTVDVPQEAFMAVLKL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QND
CCC
>Mature Secondary Structure 
TGVPVSRIRNFSIIAHIDHGKSTLADRLLQETGTVSAREMRPQLLDSMELERERGITIK
CCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEE
LQAARMNYRAQDGQDYVLNLIDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLAN
EEEEECCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
VYLALENNLEIIPVINKIDLPGAEPERVIAEIEQVIGLDCSGAILASAKVGIGIQEILEA
EEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
VVQRVPPPRDTVAEPLRALIFDSYYDAYRGVIVYVRIMDGEVKTGDKICFMASGREYEIT
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEE
ELGVMRPNQEPVDALHAGEVGYMAAAIKSVEHARVGDTITLVNRKAPEPLPGYKEAKPMV
EECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEE
FCGLFPSSSDQYAELKEALEKLKLNDAALYFEPEISSAMGFGFRCGFLGLLHMEVVQERL
EEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHH
EREYNLDLVITAPTVVYQVTLNNGSVIRVDNPSKLPPPNERTSIEEPIVRVEVILPEEFV
HHHCCCEEEEECCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHH
GTVMELCETKRGTFKDMKYLAQGRTTLVYELPLAEVVTDFFDQLKSRTRGYASMDYQMIG
HHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEEE
YRADDLVRLDILINGDVVDSLSTIVHRDKAFYVGRSLVAKLRELIPRHQFEVPIQAAIGS
ECCCCEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
KVIARETIPALRKNVLAKCYGGDVTRKRKLLEKQKEGKKRMKAVGTVDVPQEAFMAVLKL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QND
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA