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The map label for this gene is thcR [H]

Identifier: 86361019

GI number: 86361019

Start: 321663

End: 322883

Strand: Reverse

Name: thcR [H]

Synonym: RHE_PF00289

Alternate gene names: 86361019

Gene position: 322883-321663 (Counterclockwise)

Preceding gene: 86361021

Following gene: 86361016

Centisome position: 50.25

GC content: 57.9

Gene sequence:

>1221_bases
GTGGTCCAGCGGAGCGTACTTCATTTTCCAAACATATTGATTTACACTGTAGGTGTGAAGCAGGTGGAGGGGACGCTTTG
CTTCTGCGGGGCAACGAAATCATCCGTGGTTTTGCCGGTCCTGCTTAACCCATCAAGATTGGCCAGACCAGTCGCGATGA
TGTCAGGCGGCAGAAGAAAAGAACCGAGCGGCATGGATGCGTTAAGACGGGAGAAAATGGCAATTGCCGAGCATGGGGGG
TACGGATTCGAGCCCACGCATGGAGGCACCAGCTTCGAATCAATGGTTCAGGCCTTTACGGCTGGTTACGGCGTTTTCGG
CGCACAGCCGTTGAAGGATGACCGAAAATTCGCCTGGGCCGCGGATCTGAGAAGGACTGAGGCGCTCACGGTGCTTCATT
CGGTCTATCAGAGTTCCTGGACGATCCGGACGCTTGATGAGTCTCCCCAGCATCTCGCGTTTTTTATGCCGCACACAGGC
TCTTTTCGACTGTCGATAGGAAAGAGAACGGTCGAAAGTGGCGCCGGTTACTTTCTCATGGCCAACAATCACGAAGTCGG
CGATCGTGTGGTCCAGGGAGGACCGCACCGCTCGGACGCCCTTTTTCTGGACTGGAAGGTTATGAAAAGGGTCCTCGCCT
CGCTGGTGGAAAAGCCCCTATCGGAGTCGTTCGGTCTCGACCCGATCCTGGATCGCGCGACGCAGCCGGGCCAACTCATC
GGCAATCTGCTCCAGACGATCGTGCAGGGCATGCGCGGCGCCGGTCCGTTGCTATCTTCGCCTCTTGCCGCAGCCGCGAT
GAGCGAAACTCTTGCTCACCTCGTGATCCGATTTGGCCGGCATCGGTTTTCCGATCATCTGGAAAAAAAGAAAGTATCGT
TGATTGCACCTTGGCATGTCCGACGCGCTATCGACTACATGCATGCCAATATCGCGGAACCTCTCACCATGACGATGGTC
GCCGATGATGTCGGGGTTTCCCTTCGTGCGCTGCAAACGGGCTTCAAGGCCTTCAGGGGAACGTCGCCGGCCGGTTACCT
CCGCACGATCCGGCTGCAGGCGGCACGCGAGCAGTTGCGGGATCCGACGAACCAGCAATCCGTCCGCGAAATCTGCGCGA
TCTGGGGTTTTGCCCACGCGGGCAGGTTCTCCATCATTTACCGCAGCACCTTTGGAGAAAGCCCGCGCGATACACGGCTG
CGGGCTGAGCGCTTGCGCTGA

Upstream 100 bases:

>100_bases
GCTCGATGCCGGGCGACTTCCAGATCAACCGCAAAAGGTCCCGTTCGTTACGGGAGGCTACTCTCGCGGGCTCTGTGTCT
CGCAGGAAGTGTGGGGCGAC

Downstream 100 bases:

>100_bases
GGTGAGCTTAGGAGCTCGAGAGGCTGGCTCTTCCGGCATTTTAGCGCGGCGGTGCGATGCTGCCCCGCAGGACGAGATGG
CAGCCGAGCTCCAGACGATG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MVQRSVLHFPNILIYTVGVKQVEGTLCFCGATKSSVVLPVLLNPSRLARPVAMMSGGRRKEPSGMDALRREKMAIAEHGG
YGFEPTHGGTSFESMVQAFTAGYGVFGAQPLKDDRKFAWAADLRRTEALTVLHSVYQSSWTIRTLDESPQHLAFFMPHTG
SFRLSIGKRTVESGAGYFLMANNHEVGDRVVQGGPHRSDALFLDWKVMKRVLASLVEKPLSESFGLDPILDRATQPGQLI
GNLLQTIVQGMRGAGPLLSSPLAAAAMSETLAHLVIRFGRHRFSDHLEKKKVSLIAPWHVRRAIDYMHANIAEPLTMTMV
ADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPTNQQSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRL
RAERLR

Sequences:

>Translated_406_residues
MVQRSVLHFPNILIYTVGVKQVEGTLCFCGATKSSVVLPVLLNPSRLARPVAMMSGGRRKEPSGMDALRREKMAIAEHGG
YGFEPTHGGTSFESMVQAFTAGYGVFGAQPLKDDRKFAWAADLRRTEALTVLHSVYQSSWTIRTLDESPQHLAFFMPHTG
SFRLSIGKRTVESGAGYFLMANNHEVGDRVVQGGPHRSDALFLDWKVMKRVLASLVEKPLSESFGLDPILDRATQPGQLI
GNLLQTIVQGMRGAGPLLSSPLAAAAMSETLAHLVIRFGRHRFSDHLEKKKVSLIAPWHVRRAIDYMHANIAEPLTMTMV
ADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPTNQQSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRL
RAERLR
>Mature_406_residues
MVQRSVLHFPNILIYTVGVKQVEGTLCFCGATKSSVVLPVLLNPSRLARPVAMMSGGRRKEPSGMDALRREKMAIAEHGG
YGFEPTHGGTSFESMVQAFTAGYGVFGAQPLKDDRKFAWAADLRRTEALTVLHSVYQSSWTIRTLDESPQHLAFFMPHTG
SFRLSIGKRTVESGAGYFLMANNHEVGDRVVQGGPHRSDALFLDWKVMKRVLASLVEKPLSESFGLDPILDRATQPGQLI
GNLLQTIVQGMRGAGPLLSSPLAAAAMSETLAHLVIRFGRHRFSDHLEKKKVSLIAPWHVRRAIDYMHANIAEPLTMTMV
ADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPTNQQSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRL
RAERLR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=97, Percent_Identity=37.1134020618557, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 44970; Mature: 44970

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQRSVLHFPNILIYTVGVKQVEGTLCFCGATKSSVVLPVLLNPSRLARPVAMMSGGRRK
CCCCCHHHCCCEEEEECCHHHHCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHCCCCCC
EPSGMDALRREKMAIAEHGGYGFEPTHGGTSFESMVQAFTAGYGVFGAQPLKDDRKFAWA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ADLRRTEALTVLHSVYQSSWTIRTLDESPQHLAFFMPHTGSFRLSIGKRTVESGAGYFLM
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCEEEEECCHHHHCCCCEEEE
ANNHEVGDRVVQGGPHRSDALFLDWKVMKRVLASLVEKPLSESFGLDPILDRATQPGQLI
ECCCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHH
GNLLQTIVQGMRGAGPLLSSPLAAAAMSETLAHLVIRFGRHRFSDHLEKKKVSLIAPWHV
HHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHH
RRAIDYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLR
HHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DPTNQQSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRLRAERLR
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MVQRSVLHFPNILIYTVGVKQVEGTLCFCGATKSSVVLPVLLNPSRLARPVAMMSGGRRK
CCCCCHHHCCCEEEEECCHHHHCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHCCCCCC
EPSGMDALRREKMAIAEHGGYGFEPTHGGTSFESMVQAFTAGYGVFGAQPLKDDRKFAWA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ADLRRTEALTVLHSVYQSSWTIRTLDESPQHLAFFMPHTGSFRLSIGKRTVESGAGYFLM
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCEEEEECCHHHHCCCCEEEE
ANNHEVGDRVVQGGPHRSDALFLDWKVMKRVLASLVEKPLSESFGLDPILDRATQPGQLI
ECCCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHH
GNLLQTIVQGMRGAGPLLSSPLAAAAMSETLAHLVIRFGRHRFSDHLEKKKVSLIAPWHV
HHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHH
RRAIDYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLR
HHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DPTNQQSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRLRAERLR
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]