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The map label for this gene is mdeAf

Identifier: 86360753

GI number: 86360753

Start: 18139

End: 19422

Strand: Reverse

Name: mdeAf

Synonym: RHE_PF00019

Alternate gene names: 86360753

Gene position: 19422-18139 (Counterclockwise)

Preceding gene: 86360754

Following gene: 86360749

Centisome position: 3.02

GC content: 63.63

Gene sequence:

>1284_bases
ATGACCGCCCCGCATCCCTCCAAAACCCATATCGGCAATCATGCGCTGCATCCCGAAACGCAGATGCTGAACTACGGCTA
CGATCCGGAACTTTCCGAAGGGGCGGTCAAGCCGCCGGTGTTTCTGACCTCAACCTTCGTGTTCAATTCCGCCGAGGATG
GTAGGGATTTCTTCGATTATGTCTCCGGCCGTCGCGAGCCGCCGGCGGGCAAGGGCGCCGGCCTCGTCTATTCCCGCTTC
AATCATCCGAATAGCGAGATCGTCGAAGACAGGCTCGCCGTCTACGAACGGACGGAAAGCGGCGCGCTCTTCTCTTCCGG
CATGGCGGCGATCGCCACGACCCTGTTCGCCTTTGTCCGGCCAGGGGATGCGATCCTGCATTCGCAGCCGCTCTATGGCG
GCACCGAAACCCTGCTGGCGAAGACCTTCCTCAATTTCGACGTCGCCGCGGTCGGCTTTGCCGATGGCGTCAGCGAGGCA
TCGGTGCGGAAGGCGGCGGATGAGGCGGTCGGCAAGGGCCGCGTTTCCGTCATCCTCATCGAGACGCCGGCCAACCCGAC
CAACAGCCTCGTCGACGTCGCGATGATCCGGCGCGTGGCCAATGCGATCGGCGCAAGGCAAGGGCATGTGCCGGTCGTCG
TCTGCGACAATACGCTGCTCGGGCCGGTCTTCCAGCGGCCGATCGAGCATGGCGCCGATATCTCGCTTTATTCGCTGACC
AAATATGTCGGTGGCCATTCCGATCTGATCGCCGGCGCCGTGCTCGGGCGCAAGGCTGTCATCAAGCAGGTCAAGGCGCT
GCGCGGCGCGATCGGCACGCAGCTCGATCCGCATTCCTGCTGGATGCTCGGCCGGTCGCTCGAAACGCTGCAGCTACGCA
TGGAGCGGGCCAACAGCAATGCGCGGGCAGTGGCCGATTTCCTGCGTGATCATCCCAAGGTCGAGAAGGTTCATTATCTT
CCCTATCACGATCCGGACTCGCCCTCCGGCCGCACCTTCGCCGCCCAATGCAGCGGCGCCGGCTCGACCTTCTCCTTCGA
TATCCGCGGCGGCCAGCCGGCCTCCTTCAAATTCCTGAATGCACTGCAGGTCTTCAAGCTCGCCGTGAGCCTCGGCGGCA
CGGAATCGCTGGCGAGCCACCCCGCCGCCATGACGCATTCCGGCGTGCCGGCCGATGTCAGGGAGCGCATCGGCGTGCTG
GAATCGACAATCCGGCTGTCGATCGGCATCGAGCATCCGGATGATTTGATCGCCGATCTGGAGCTGGCGCTGCAGGCTGT
TTGA

Upstream 100 bases:

>100_bases
GATGCCGAAAGCATGCTCCACTCTTAATTGAGCAGTCGCGATGACTGCTCCTCGAATTGATGTATCCTGTCCTTCGGCAG
GTTCGACAGAGGAGACGATC

Downstream 100 bases:

>100_bases
CGCGCTCGCATGGAATCGATGCCCGGCGGCGCCGGGCATCGAGGACGAGGTCACTTGTCGTCGGTCGTCAGTTCCGTCTC
GTCGCTATTGAGCACGAACA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDYVSGRREPPAGKGAGLVYSRF
NHPNSEIVEDRLAVYERTESGALFSSGMAAIATTLFAFVRPGDAILHSQPLYGGTETLLAKTFLNFDVAAVGFADGVSEA
SVRKAADEAVGKGRVSVILIETPANPTNSLVDVAMIRRVANAIGARQGHVPVVVCDNTLLGPVFQRPIEHGADISLYSLT
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYL
PYHDPDSPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLNALQVFKLAVSLGGTESLASHPAAMTHSGVPADVRERIGVL
ESTIRLSIGIEHPDDLIADLELALQAV

Sequences:

>Translated_427_residues
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDYVSGRREPPAGKGAGLVYSRF
NHPNSEIVEDRLAVYERTESGALFSSGMAAIATTLFAFVRPGDAILHSQPLYGGTETLLAKTFLNFDVAAVGFADGVSEA
SVRKAADEAVGKGRVSVILIETPANPTNSLVDVAMIRRVANAIGARQGHVPVVVCDNTLLGPVFQRPIEHGADISLYSLT
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYL
PYHDPDSPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLNALQVFKLAVSLGGTESLASHPAAMTHSGVPADVRERIGVL
ESTIRLSIGIEHPDDLIADLELALQAV
>Mature_426_residues
TAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDYVSGRREPPAGKGAGLVYSRFN
HPNSEIVEDRLAVYERTESGALFSSGMAAIATTLFAFVRPGDAILHSQPLYGGTETLLAKTFLNFDVAAVGFADGVSEAS
VRKAADEAVGKGRVSVILIETPANPTNSLVDVAMIRRVANAIGARQGHVPVVVCDNTLLGPVFQRPIEHGADISLYSLTK
YVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYLP
YHDPDSPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLNALQVFKLAVSLGGTESLASHPAAMTHSGVPADVRERIGVLE
STIRLSIGIEHPDDLIADLELALQAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=400, Percent_Identity=34.25, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI299473759, Length=401, Percent_Identity=31.4214463840399, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI25453487, Length=400, Percent_Identity=30.5, Blast_Score=165, Evalue=8e-41,
Organism=Escherichia coli, GI1790375, Length=402, Percent_Identity=34.8258706467662, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1789383, Length=413, Percent_Identity=25.90799031477, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=406, Percent_Identity=33.743842364532, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=406, Percent_Identity=33.2512315270936, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=420, Percent_Identity=31.6666666666667, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=34.4139650872818, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=29.1284403669725, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=409, Percent_Identity=28.8508557457213, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=428, Percent_Identity=26.6355140186916, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=25.2808988764045, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19922554, Length=411, Percent_Identity=36.7396593673966, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45703; Mature: 45572

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDY
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHH
VSGRREPPAGKGAGLVYSRFNHPNSEIVEDRLAVYERTESGALFSSGMAAIATTLFAFVR
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHC
PGDAILHSQPLYGGTETLLAKTFLNFDVAAVGFADGVSEASVRKAADEAVGKGRVSVILI
CCCHHEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEE
ETPANPTNSLVDVAMIRRVANAIGARQGHVPVVVCDNTLLGPVFQRPIEHGADISLYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
ARAVADFLRDHPKVEKVHYLPYHDPDSPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHH
ALQVFKLAVSLGGTESLASHPAAMTHSGVPADVRERIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
ELALQAV
HHHHHCC
>Mature Secondary Structure 
TAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDY
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHH
VSGRREPPAGKGAGLVYSRFNHPNSEIVEDRLAVYERTESGALFSSGMAAIATTLFAFVR
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHC
PGDAILHSQPLYGGTETLLAKTFLNFDVAAVGFADGVSEASVRKAADEAVGKGRVSVILI
CCCHHEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEE
ETPANPTNSLVDVAMIRRVANAIGARQGHVPVVVCDNTLLGPVFQRPIEHGADISLYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
ARAVADFLRDHPKVEKVHYLPYHDPDSPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHH
ALQVFKLAVSLGGTESLASHPAAMTHSGVPADVRERIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
ELALQAV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]