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The map label for this gene is mltD [H]

Identifier: 85859386

GI number: 85859386

Start: 1589039

End: 1591003

Strand: Direct

Name: mltD [H]

Synonym: SYN_02791

Alternate gene names: 85859386

Gene position: 1589039-1591003 (Clockwise)

Preceding gene: 85859385

Following gene: 85859395

Centisome position: 49.98

GC content: 41.58

Gene sequence:

>1965_bases
ATGAAAAAGGCAACTCTGTATAAAATTACCCATATCATTTTTATGTTTCTGTTATCATTATATTTTACACACAGCGTTGG
TGCTCAAACTCAAAATGATAATGAAGGCAAGGATGGAAAAGCAACGAGCAAACAAAATTCCTCTTCACTGCAGAATATGA
AGAAAAATTCGAGCATGAAAGATGTACAGATAAATTTGCCATTATTCGCAAAGTTAGCCAGCCCTTCTCCTGACGAAGAT
TTCGATGAGGAGGATAAAGAGGGTGACCGGGACCTCATGGAAGAAGCACTTACTTACCTGAACCAGTCTCAAAAACTGTG
GGAGAAAGGTGATATTGAAAAAGCACTGGATTTTCTTGACCAGGCTTATACGCTGGTTCTGGAAACCGATGGAGATCTGG
AAATCGCACGTCAGAAAGATGATCTGCGCTTACTGATATCAAAGAGGATACTTGCCATTTATTCGTCGATTCAGTCAACG
ACTCTGGGAAAACGCAGCGAGATTCCGTTGGCAATGAATTCAGATGTTGAAAGAGAAATTCGATCCTTTCAGACCTGTGA
AAAGGATTTTTTTGTATCATCCTATCAGAGATCCGGAATTTACCGGGACAACATCGTAAAGGAATTAAAAAAAGCCGGTT
TGCCGGAGGAGCTATCTTGGTTGCCTCTTGTTGAAAGTGGATTTAAAATCGGTGCCCTATCGCGAGCTCGTGCTCTTGGC
CTCTGGCAATTCATTCCGTCTACCGGATATAAATTTGGTTTGAATCGCGATGAATGGGTCGATGAGCGAATGGATGCGGA
AAAATCGACGAGGGCGGCAATCAGTTACTTGAAGGAATTGCATGGGATGTTTGGAGACTGGCTGACCGTGCTGGCCGCTT
ACAACAGTGGTGAAGGCAGAGTGATGAGAGTCATATCCAGACAGCATATCAACTATCTCGACCGGTTCTGGGATCTTTAT
CATCAGTTGCCCAATGAAACGGCTCGCTATGTTCCGCGTTTTCTGGCAACTCTGCATATCATTCGGGACCCCAAGAAATA
TGGTTTCGATCTGAAAACAGACATGGAACAGACGTCGCCTTATGAATATAAGATTGTAAAATCTTATAAACCTATGAAGC
TGCAGGACGTTGCCTTTTATACAGGCGGTTCTGAAGATGTTTTAACCGGAATGAATTCAGAGTTGCGTCATAAGATGACG
CCGGATAAGGAATACGATCTTAAGCTTCCACCGGAGGCCGTTGAAAAATATGCTCAGATTATCGATCAAATTCCCCAGTC
TGAAAAACCAAACAATGTTTCCATGGTATCGTCATTAAATCGTGGAGACCACAAATCCGAACGTGTATCTGAACGGCCTG
CGCCAAAATCAAGATATTTGAATTACAGGGTAAAGCGGGGGGAATCACTCAATACAATCGCGAAAAAGTATGGAATATCT
CCTCGTACACTGAGAGCCAGCAATCATCTTTCTTCGAGGAACAAGTTAAAGGCAGGGCATAAAATTCGTATCCCGATAGC
GAATTCAAGAATATTAAAAAGAGAATCCGTTCAAATTGTGGGTGCAGGCCAGAGTAAAAATTCACTCGCGAAGGAGACGC
TGATTCGTTATAAGGTAAAAAAAGGAGATTCTCTTTCATCCTTGGCACAGCGATTTGGCACAAGCGAAACAGAAATAAAA
AAGATGAACCGCATCAAAGGAAAATATCTGACAACCGGAAGTGTGATCAAAATAAGCCGATTCAGTGATGATGATTCTGT
TAGTGAACCCAAAAGAGATTTAAGCAGAAAGTCAAATAAACGGGTGAGTTCGAGAGCAAAAGAAAAGACTTATCTGGTTA
AAAAAGGGGACAATCTTTCCCTGATTGCCCAGAAAAACAACATGAGTCTTGATAAACTTAAAGAAATAAACAATATCGCG
AAAAGAGAAAATCTTCGCCCGGGACAGATTTTGATTATTCAATAA

Upstream 100 bases:

>100_bases
ACAATACACGGGATCATTCAGGATCATTCAAAAAAATATCAAGCCCTCTGCACATTGAAACCAAACATTAGAGGTTCTTT
TCAGGAAGGAGTTTCACCTC

Downstream 100 bases:

>100_bases
TAAAAATCTGTACGAAAAATAAATAAAATGATGATGTGTTAAAAATCGAGAGGATATCCATGGAGCAGGAAAAATAATTC
CTGCTCTTTTTTTTGCATGC

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MKKATLYKITHIIFMFLLSLYFTHSVGAQTQNDNEGKDGKATSKQNSSSLQNMKKNSSMKDVQINLPLFAKLASPSPDED
FDEEDKEGDRDLMEEALTYLNQSQKLWEKGDIEKALDFLDQAYTLVLETDGDLEIARQKDDLRLLISKRILAIYSSIQST
TLGKRSEIPLAMNSDVEREIRSFQTCEKDFFVSSYQRSGIYRDNIVKELKKAGLPEELSWLPLVESGFKIGALSRARALG
LWQFIPSTGYKFGLNRDEWVDERMDAEKSTRAAISYLKELHGMFGDWLTVLAAYNSGEGRVMRVISRQHINYLDRFWDLY
HQLPNETARYVPRFLATLHIIRDPKKYGFDLKTDMEQTSPYEYKIVKSYKPMKLQDVAFYTGGSEDVLTGMNSELRHKMT
PDKEYDLKLPPEAVEKYAQIIDQIPQSEKPNNVSMVSSLNRGDHKSERVSERPAPKSRYLNYRVKRGESLNTIAKKYGIS
PRTLRASNHLSSRNKLKAGHKIRIPIANSRILKRESVQIVGAGQSKNSLAKETLIRYKVKKGDSLSSLAQRFGTSETEIK
KMNRIKGKYLTTGSVIKISRFSDDDSVSEPKRDLSRKSNKRVSSRAKEKTYLVKKGDNLSLIAQKNNMSLDKLKEINNIA
KRENLRPGQILIIQ

Sequences:

>Translated_654_residues
MKKATLYKITHIIFMFLLSLYFTHSVGAQTQNDNEGKDGKATSKQNSSSLQNMKKNSSMKDVQINLPLFAKLASPSPDED
FDEEDKEGDRDLMEEALTYLNQSQKLWEKGDIEKALDFLDQAYTLVLETDGDLEIARQKDDLRLLISKRILAIYSSIQST
TLGKRSEIPLAMNSDVEREIRSFQTCEKDFFVSSYQRSGIYRDNIVKELKKAGLPEELSWLPLVESGFKIGALSRARALG
LWQFIPSTGYKFGLNRDEWVDERMDAEKSTRAAISYLKELHGMFGDWLTVLAAYNSGEGRVMRVISRQHINYLDRFWDLY
HQLPNETARYVPRFLATLHIIRDPKKYGFDLKTDMEQTSPYEYKIVKSYKPMKLQDVAFYTGGSEDVLTGMNSELRHKMT
PDKEYDLKLPPEAVEKYAQIIDQIPQSEKPNNVSMVSSLNRGDHKSERVSERPAPKSRYLNYRVKRGESLNTIAKKYGIS
PRTLRASNHLSSRNKLKAGHKIRIPIANSRILKRESVQIVGAGQSKNSLAKETLIRYKVKKGDSLSSLAQRFGTSETEIK
KMNRIKGKYLTTGSVIKISRFSDDDSVSEPKRDLSRKSNKRVSSRAKEKTYLVKKGDNLSLIAQKNNMSLDKLKEINNIA
KRENLRPGQILIIQ
>Mature_654_residues
MKKATLYKITHIIFMFLLSLYFTHSVGAQTQNDNEGKDGKATSKQNSSSLQNMKKNSSMKDVQINLPLFAKLASPSPDED
FDEEDKEGDRDLMEEALTYLNQSQKLWEKGDIEKALDFLDQAYTLVLETDGDLEIARQKDDLRLLISKRILAIYSSIQST
TLGKRSEIPLAMNSDVEREIRSFQTCEKDFFVSSYQRSGIYRDNIVKELKKAGLPEELSWLPLVESGFKIGALSRARALG
LWQFIPSTGYKFGLNRDEWVDERMDAEKSTRAAISYLKELHGMFGDWLTVLAAYNSGEGRVMRVISRQHINYLDRFWDLY
HQLPNETARYVPRFLATLHIIRDPKKYGFDLKTDMEQTSPYEYKIVKSYKPMKLQDVAFYTGGSEDVLTGMNSELRHKMT
PDKEYDLKLPPEAVEKYAQIIDQIPQSEKPNNVSMVSSLNRGDHKSERVSERPAPKSRYLNYRVKRGESLNTIAKKYGIS
PRTLRASNHLSSRNKLKAGHKIRIPIANSRILKRESVQIVGAGQSKNSLAKETLIRYKVKKGDSLSSLAQRFGTSETEIK
KMNRIKGKYLTTGSVIKISRFSDDDSVSEPKRDLSRKSNKRVSSRAKEKTYLVKKGDNLSLIAQKNNMSLDKLKEINNIA
KRENLRPGQILIIQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=362, Percent_Identity=34.8066298342541, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74838; Mature: 74838

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKATLYKITHIIFMFLLSLYFTHSVGAQTQNDNEGKDGKATSKQNSSSLQNMKKNSSMK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
DVQINLPLFAKLASPSPDEDFDEEDKEGDRDLMEEALTYLNQSQKLWEKGDIEKALDFLD
EEEECCCHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QAYTLVLETDGDLEIARQKDDLRLLISKRILAIYSSIQSTTLGKRSEIPLAMNSDVEREI
HHEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHH
RSFQTCEKDFFVSSYQRSGIYRDNIVKELKKAGLPEELSWLPLVESGFKIGALSRARALG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
LWQFIPSTGYKFGLNRDEWVDERMDAEKSTRAAISYLKELHGMFGDWLTVLAAYNSGEGR
HHHHHCCCCCEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VMRVISRQHINYLDRFWDLYHQLPNETARYVPRFLATLHIIRDPKKYGFDLKTDMEQTSP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCC
YEYKIVKSYKPMKLQDVAFYTGGSEDVLTGMNSELRHKMTPDKEYDLKLPPEAVEKYAQI
CEEEEECCCCCCEEEEEEEEECCCHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IDQIPQSEKPNNVSMVSSLNRGDHKSERVSERPAPKSRYLNYRVKRGESLNTIAKKYGIS
HHHCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHCEEHCCCCCCHHHHHHHHCCC
PRTLRASNHLSSRNKLKAGHKIRIPIANSRILKRESVQIVGAGQSKNSLAKETLIRYKVK
CCEEECHHHCCCHHHHCCCCEEEEEECCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHC
KGDSLSSLAQRFGTSETEIKKMNRIKGKYLTTGSVIKISRFSDDDSVSEPKRDLSRKSNK
CCCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCHHHHHHHHHHHH
RVSSRAKEKTYLVKKGDNLSLIAQKNNMSLDKLKEINNIAKRENLRPGQILIIQ
HHHHHHHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure
MKKATLYKITHIIFMFLLSLYFTHSVGAQTQNDNEGKDGKATSKQNSSSLQNMKKNSSMK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
DVQINLPLFAKLASPSPDEDFDEEDKEGDRDLMEEALTYLNQSQKLWEKGDIEKALDFLD
EEEECCCHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QAYTLVLETDGDLEIARQKDDLRLLISKRILAIYSSIQSTTLGKRSEIPLAMNSDVEREI
HHEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHH
RSFQTCEKDFFVSSYQRSGIYRDNIVKELKKAGLPEELSWLPLVESGFKIGALSRARALG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
LWQFIPSTGYKFGLNRDEWVDERMDAEKSTRAAISYLKELHGMFGDWLTVLAAYNSGEGR
HHHHHCCCCCEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VMRVISRQHINYLDRFWDLYHQLPNETARYVPRFLATLHIIRDPKKYGFDLKTDMEQTSP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHCCC
YEYKIVKSYKPMKLQDVAFYTGGSEDVLTGMNSELRHKMTPDKEYDLKLPPEAVEKYAQI
CEEEEECCCCCCEEEEEEEEECCCHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IDQIPQSEKPNNVSMVSSLNRGDHKSERVSERPAPKSRYLNYRVKRGESLNTIAKKYGIS
HHHCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHCEEHCCCCCCHHHHHHHHCCC
PRTLRASNHLSSRNKLKAGHKIRIPIANSRILKRESVQIVGAGQSKNSLAKETLIRYKVK
CCEEECHHHCCCHHHHCCCCEEEEEECCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHC
KGDSLSSLAQRFGTSETEIKKMNRIKGKYLTTGSVIKISRFSDDDSVSEPKRDLSRKSNK
CCCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCCEEEEEECCCCCCCCHHHHHHHHHHHH
RVSSRAKEKTYLVKKGDNLSLIAQKNNMSLDKLKEINNIAKRENLRPGQILIIQ
HHHHHHHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]