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The map label for this gene is prfB [H]

Identifier: 85859385

GI number: 85859385

Start: 1587973

End: 1588812

Strand: Direct

Name: prfB [H]

Synonym: SYN_02790

Alternate gene names: 85859385

Gene position: 1587973-1588812 (Clockwise)

Preceding gene: 85859384

Following gene: 85859386

Centisome position: 49.95

GC content: 45.36

Gene sequence:

>840_bases
ATGGCCAAGGAAGAAAATGACGAAGAGGCCTTGGAAGCTATCTCGAATGATATAGAATCATTAAGGAAATCAGTGCGGGA
AAGCGAACTGAAAATGATGCTTGGCAGTGAGCAGGATCCCATGAATGCCATTGTCTCCATACATGCGGGCGCGGGGGGGA
CGGAGGCTCAGGATTGGGTGGAAATGCTGTTGAGAATGTATCTCCGATGGGCGGAGAAACGAGGTTTTAAAACGACAATT
ATTGATTATCTGCCCGGTGATGAAGCCGGGGTTAAAAGTGTTTCCTTTACGATTGAGGGTGAATATGCCTATGGTTACGC
AAAAGCGGAGATTGGCATTCACAGGCTGGTCAGAATATCACCCTTCGATGCCAGCTCACGACGTCATACTTCTTTTGCCT
CGGTTTTCGTTTATCCTGAAGTCAATGACGAAATCGTCGTGGAAATCGATGAAAAGGATTTAAGAATCGATACATACAGA
TCAACCGGTGCGGGCGGACAACATGTGAACAAAACCGATTCCGCAGTCAGAATTACCCATATGCCGACGGGTATTGTTGT
TCAGTGTCAAAATGAGAGATCTCAGCATAAAAACAAGGCTATGGCAATGAAATATCTGAGATCAAGGCTTTACGAACTCA
AAATTCAGGAACAGAATGAAAAGCTCTCCGAAATCAACAAGACGAAAAAGGACATCGCCTGGGGGAGTCAGATTCGGTCC
TATGTTCTCCAGCCTTACAAGATGGTCAAAGATCACAGAACCAATATCGAAATCGGAAATGTCGGCAGAGTTTTAGACGG
TGACATTGACGATCTGATCGAGGCTTATCTCCTCCAGTAA

Upstream 100 bases:

>100_bases
GGCAACGTCAATTCTGAAGGAAAAGAACAACCTGACGGCGACAGTTGATGCATGGCATACCCAGCAAAGAGCTATTCTTG
ATATTGAGTTTCTTTATGAC

Downstream 100 bases:

>100_bases
ATTGATGACCGGAATTCCTTATAATTTCGTGATATCAACACATACGGGGATGATGCTCCTGTATTTCGTCATTTTTTCTT
AGAAGTATGGAAAAAATGTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAKEENDEEALEAISNDIESLRKSVRESELKMMLGSEQDPMNAIVSIHAGAGGTEAQDWVEMLLRMYLRWAEKRGFKTTI
IDYLPGDEAGVKSVSFTIEGEYAYGYAKAEIGIHRLVRISPFDASSRRHTSFASVFVYPEVNDEIVVEIDEKDLRIDTYR
STGAGGQHVNKTDSAVRITHMPTGIVVQCQNERSQHKNKAMAMKYLRSRLYELKIQEQNEKLSEINKTKKDIAWGSQIRS
YVLQPYKMVKDHRTNIEIGNVGRVLDGDIDDLIEAYLLQ

Sequences:

>Translated_279_residues
MAKEENDEEALEAISNDIESLRKSVRESELKMMLGSEQDPMNAIVSIHAGAGGTEAQDWVEMLLRMYLRWAEKRGFKTTI
IDYLPGDEAGVKSVSFTIEGEYAYGYAKAEIGIHRLVRISPFDASSRRHTSFASVFVYPEVNDEIVVEIDEKDLRIDTYR
STGAGGQHVNKTDSAVRITHMPTGIVVQCQNERSQHKNKAMAMKYLRSRLYELKIQEQNEKLSEINKTKKDIAWGSQIRS
YVLQPYKMVKDHRTNIEIGNVGRVLDGDIDDLIEAYLLQ
>Mature_278_residues
AKEENDEEALEAISNDIESLRKSVRESELKMMLGSEQDPMNAIVSIHAGAGGTEAQDWVEMLLRMYLRWAEKRGFKTTII
DYLPGDEAGVKSVSFTIEGEYAYGYAKAEIGIHRLVRISPFDASSRRHTSFASVFVYPEVNDEIVVEIDEKDLRIDTYRS
TGAGGQHVNKTDSAVRITHMPTGIVVQCQNERSQHKNKAMAMKYLRSRLYELKIQEQNEKLSEINKTKKDIAWGSQIRSY
VLQPYKMVKDHRTNIEIGNVGRVLDGDIDDLIEAYLLQ

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=283, Percent_Identity=36.3957597173145, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI34577120, Length=250, Percent_Identity=39.2, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=36.0655737704918, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI2367172, Length=277, Percent_Identity=55.5956678700361, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=41.7355371900826, Blast_Score=188, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=280, Percent_Identity=33.9285714285714, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=247, Percent_Identity=37.246963562753, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=215, Percent_Identity=42.3255813953488, Blast_Score=158, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 31737; Mature: 31606

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEENDEEALEAISNDIESLRKSVRESELKMMLGSEQDPMNAIVSIHAGAGGTEAQDWV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHH
EMLLRMYLRWAEKRGFKTTIIDYLPGDEAGVKSVSFTIEGEYAYGYAKAEIGIHRLVRIS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEECCEEEEEEHHHHHHHHEEEEC
PFDASSRRHTSFASVFVYPEVNDEIVVEIDEKDLRIDTYRSTGAGGQHVNKTDSAVRITH
CCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEE
MPTGIVVQCQNERSQHKNKAMAMKYLRSRLYELKIQEQNEKLSEINKTKKDIAWGSQIRS
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YVLQPYKMVKDHRTNIEIGNVGRVLDGDIDDLIEAYLLQ
HHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKEENDEEALEAISNDIESLRKSVRESELKMMLGSEQDPMNAIVSIHAGAGGTEAQDWV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHH
EMLLRMYLRWAEKRGFKTTIIDYLPGDEAGVKSVSFTIEGEYAYGYAKAEIGIHRLVRIS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEECCEEEEEEHHHHHHHHEEEEC
PFDASSRRHTSFASVFVYPEVNDEIVVEIDEKDLRIDTYRSTGAGGQHVNKTDSAVRITH
CCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEE
MPTGIVVQCQNERSQHKNKAMAMKYLRSRLYELKIQEQNEKLSEINKTKKDIAWGSQIRS
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YVLQPYKMVKDHRTNIEIGNVGRVLDGDIDDLIEAYLLQ
HHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA