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The map label for this gene is bioB [C]

Identifier: 85859383

GI number: 85859383

Start: 1584887

End: 1585801

Strand: Direct

Name: bioB [C]

Synonym: SYN_02788

Alternate gene names: 85859383

Gene position: 1584887-1585801 (Clockwise)

Preceding gene: 85859382

Following gene: 85859384

Centisome position: 49.85

GC content: 42.84

Gene sequence:

>915_bases
TTGAAAGGAAAACAGGTAACAAACATTAAAACAGGTGCCGCCATGAAATACGAGGGATCAATTTACCGTCCACCAAGTGA
GGCATACAGCTTGTTGATTCAGGTTACAACGGGTTGCTCACATAATAAGTGTACTTTTTGCCCTCTTTATAAAGACAAGA
GATTTAAGATAAAGAGTTTTCAGGAAATCGAGGAGGATATCAGAGAGGCTGCAAGATATCGTTCCGTTGGAAAGGTATTT
CTCTGTGACGGGGATGCCCTGATCATCCCCCAGAAAAAACTGGTGCCCATTTTTGAATCCATCAATCGCAATCTCCGAGG
AGTTGAACGGATCGGTCTTTATGGAAACGCAAAGAGCATTCTTAGAAAAACTCCGGATGAATTAGTTCAACTAAAAAACC
TGGGTTTAAAGATTGTTTATCTTGGTGTTGAAACCGGAAATTCCGAATTGCTTGAAAAGATCAGGAAAGGCGTCACATAT
TCACAGCTTGTGGAAGCAGGGCGGAAAGTGAAAGAAGCAGGAATCCTTCTTTCCGTAACGGTATTGCTGGGAATCGGCGG
TGTCGAAAAGAGTATGGAGCATGCTCGTGATACCGCCCGTATTTTAAGTGATATCGACCCGGATTACGCCAGTGCCTTAA
CACTTATGATGGTACCGGGAACCCCGATCTATGATGATTTTATTCAGGGAAAATTTGTTATTCCGGACCAGTATGGATGC
CTCAAAGAGATTGGAATGATCCTTGAGCAATCTAATTTTACTGACTGTTTCTTCACGTCGAATCACGCCTCAAATTATCT
GCCTGTAAAAGCAAGAATGCCAAAGGAAAAGGAAAAGGTCGTCCGGTTAATCGAGGAAGTCATTCAAAAAGGGCAAAGCA
GTGTTCTAAGATCTGAAAACATGCGCGCCCTGTAG

Upstream 100 bases:

>100_bases
GCCGCTTTAAGTCGGATTCTCTCCTTTACTGTTTGAATATTTTAACCGTTCCTGCTATTCATTATATTGTTTTTTCGGCA
ATCTTTTCCTGTAAACCTTT

Downstream 100 bases:

>100_bases
AAAAAATTGTAAAAACGGAACTGGATGAATTTCAAAACGAAGAATGAAACATTTTGCCGATAATTCTTTCTCGATAAAAA
ATCGTAATATTCAAGAAATC

Product: oxygen-independent coproporphyrinogen III oxidase, Fe-S oxidoreductase

Products: Biotin [C]

Alternate protein names: Radical SAM Domain Protein; Fe-S Oxidoreductase; Oxygen-Independent Coproporphyrinogen III Oxidase; Radical SAM Family Protein; Radical Sam Domain Protein; Coproporphyrinogen Oxidase; Elongator /MiaB/NifB; Fe-S Oxidoreductase Family Protein; Radical SAM Superfamily Lipoprotein; Oxygen-Independent Coproporphyrinogen III; Radical SAM

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKGKQVTNIKTGAAMKYEGSIYRPPSEAYSLLIQVTTGCSHNKCTFCPLYKDKRFKIKSFQEIEEDIREAARYRSVGKVF
LCDGDALIIPQKKLVPIFESINRNLRGVERIGLYGNAKSILRKTPDELVQLKNLGLKIVYLGVETGNSELLEKIRKGVTY
SQLVEAGRKVKEAGILLSVTVLLGIGGVEKSMEHARDTARILSDIDPDYASALTLMMVPGTPIYDDFIQGKFVIPDQYGC
LKEIGMILEQSNFTDCFFTSNHASNYLPVKARMPKEKEKVVRLIEEVIQKGQSSVLRSENMRAL

Sequences:

>Translated_304_residues
MKGKQVTNIKTGAAMKYEGSIYRPPSEAYSLLIQVTTGCSHNKCTFCPLYKDKRFKIKSFQEIEEDIREAARYRSVGKVF
LCDGDALIIPQKKLVPIFESINRNLRGVERIGLYGNAKSILRKTPDELVQLKNLGLKIVYLGVETGNSELLEKIRKGVTY
SQLVEAGRKVKEAGILLSVTVLLGIGGVEKSMEHARDTARILSDIDPDYASALTLMMVPGTPIYDDFIQGKFVIPDQYGC
LKEIGMILEQSNFTDCFFTSNHASNYLPVKARMPKEKEKVVRLIEEVIQKGQSSVLRSENMRAL
>Mature_304_residues
MKGKQVTNIKTGAAMKYEGSIYRPPSEAYSLLIQVTTGCSHNKCTFCPLYKDKRFKIKSFQEIEEDIREAARYRSVGKVF
LCDGDALIIPQKKLVPIFESINRNLRGVERIGLYGNAKSILRKTPDELVQLKNLGLKIVYLGVETGNSELLEKIRKGVTY
SQLVEAGRKVKEAGILLSVTVLLGIGGVEKSMEHARDTARILSDIDPDYASALTLMMVPGTPIYDDFIQGKFVIPDQYGC
LKEIGMILEQSNFTDCFFTSNHASNYLPVKARMPKEKEKVVRLIEEVIQKGQSSVLRSENMRAL

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6 [C]

Molecular weight: Translated: 34079; Mature: 34079

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKQVTNIKTGAAMKYEGSIYRPPSEAYSLLIQVTTGCSHNKCTFCPLYKDKRFKIKSF
CCCCEECCCCCCCEEEECCCEECCCHHHHEEEEEEECCCCCCCEEEEECCCCCCCCHHHH
QEIEEDIREAARYRSVGKVFLCDGDALIIPQKKLVPIFESINRNLRGVERIGLYGNAKSI
HHHHHHHHHHHHHHCCCEEEEECCCEEEECCHHHHHHHHHHCCCCCHHHHHCCCCCHHHH
LRKTPDELVQLKNLGLKIVYLGVETGNSELLEKIRKGVTYSQLVEAGRKVKEAGILLSVT
HHCCHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
VLLGIGGVEKSMEHARDTARILSDIDPDYASALTLMMVPGTPIYDDFIQGKFVIPDQYGC
HHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCHHHHHCCEEECCCCCHH
LKEIGMILEQSNFTDCFFTSNHASNYLPVKARMPKEKEKVVRLIEEVIQKGQSSVLRSEN
HHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
MRAL
CCCC
>Mature Secondary Structure
MKGKQVTNIKTGAAMKYEGSIYRPPSEAYSLLIQVTTGCSHNKCTFCPLYKDKRFKIKSF
CCCCEECCCCCCCEEEECCCEECCCHHHHEEEEEEECCCCCCCEEEEECCCCCCCCHHHH
QEIEEDIREAARYRSVGKVFLCDGDALIIPQKKLVPIFESINRNLRGVERIGLYGNAKSI
HHHHHHHHHHHHHHCCCEEEEECCCEEEECCHHHHHHHHHHCCCCCHHHHHCCCCCHHHH
LRKTPDELVQLKNLGLKIVYLGVETGNSELLEKIRKGVTYSQLVEAGRKVKEAGILLSVT
HHCCHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
VLLGIGGVEKSMEHARDTARILSDIDPDYASALTLMMVPGTPIYDDFIQGKFVIPDQYGC
HHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCHHHHHCCEEECCCCCHH
LKEIGMILEQSNFTDCFFTSNHASNYLPVKARMPKEKEKVVRLIEEVIQKGQSSVLRSEN
HHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
MRAL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: Dethiobiotin; sulfur [C]

Specific reaction: Dethiobiotin + sulfur = biotin [C]

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA