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The map label for this gene is folK [H]

Identifier: 85859280

GI number: 85859280

Start: 1467572

End: 1468102

Strand: Direct

Name: folK [H]

Synonym: SYN_02163

Alternate gene names: 85859280

Gene position: 1467572-1468102 (Clockwise)

Preceding gene: 85859279

Following gene: 85859281

Centisome position: 46.16

GC content: 50.09

Gene sequence:

>531_bases
GTGGATAAAGCAGGGAATCCTTTGTGTGAAGAAGTTTTGGGGGTCATTGCCTATATCGGCATCGGATCGAATCTTGGGCA
GCCCTTGGAACAGTGTCTCGAAGCCATTAAGTGTCTTTCGTCTGTCAACGCCATTACGCTCCTGCGTTGTTCCTCTCTTT
ACAGGACTGAACCGATAGGGTTGAAAAATCAGCCCTGGTTTGTTAATGCCGTTGCTGAAATACGTACATCTCTTTCACCG
CGAGCGCTTTTGCGAATCCTCATGCAGATTGAGGAAGCTATGGGGAGAGTTCGGGAAGCGCATTGGGGTCCACGGATCAT
TGATCTGGATATTCTCCTGTTCGGGCAGGAGATCGTTCAGGAGCAGGATCTGAAGATTCCTCATCCCGAACTGCATAAAA
GGCGTTTTGTCCTTATCCCGCTGAGCGAACTGGCCTCCTATGCGATTCATCCGGCTTTCGGGGTATCGATTCGTGGACTC
ATGGAGAGGCTTGAGGACGACAGCCGGGTTGAACGCATTGAATATGGTTAA

Upstream 100 bases:

>100_bases
TGGGTGGTCAGGCAGGAACCGGGACTCTATGATATGCAGGATGTCCTCGGGTTCCGATCCTGAAAAAGAACTTTGTATAT
CAGACCATGGTCAGTGAGCT

Downstream 100 bases:

>100_bases
ATGAGCATGATTCTTCGGTTTATTTTTACAGTTATTGTTCTTTATCTGATTTTTCGGGTGTTAAAGAGAATTCTGCGTTC
TGCGGAAAAGAAGAAAAATG

Product: 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK)

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDKAGNPLCEEVLGVIAYIGIGSNLGQPLEQCLEAIKCLSSVNAITLLRCSSLYRTEPIGLKNQPWFVNAVAEIRTSLSP
RALLRILMQIEEAMGRVREAHWGPRIIDLDILLFGQEIVQEQDLKIPHPELHKRRFVLIPLSELASYAIHPAFGVSIRGL
MERLEDDSRVERIEYG

Sequences:

>Translated_176_residues
MDKAGNPLCEEVLGVIAYIGIGSNLGQPLEQCLEAIKCLSSVNAITLLRCSSLYRTEPIGLKNQPWFVNAVAEIRTSLSP
RALLRILMQIEEAMGRVREAHWGPRIIDLDILLFGQEIVQEQDLKIPHPELHKRRFVLIPLSELASYAIHPAFGVSIRGL
MERLEDDSRVERIEYG
>Mature_176_residues
MDKAGNPLCEEVLGVIAYIGIGSNLGQPLEQCLEAIKCLSSVNAITLLRCSSLYRTEPIGLKNQPWFVNAVAEIRTSLSP
RALLRILMQIEEAMGRVREAHWGPRIIDLDILLFGQEIVQEQDLKIPHPELHKRRFVLIPLSELASYAIHPAFGVSIRGL
MERLEDDSRVERIEYG

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=138, Percent_Identity=45.6521739130435, Blast_Score=127, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324073, Length=162, Percent_Identity=38.2716049382716, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 19812; Mature: 19812

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAGNPLCEEVLGVIAYIGIGSNLGQPLEQCLEAIKCLSSVNAITLLRCSSLYRTEPIG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
LKNQPWFVNAVAEIRTSLSPRALLRILMQIEEAMGRVREAHWGPRIIDLDILLFGQEIVQ
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
EQDLKIPHPELHKRRFVLIPLSELASYAIHPAFGVSIRGLMERLEDDSRVERIEYG
HCCCCCCCHHHHCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MDKAGNPLCEEVLGVIAYIGIGSNLGQPLEQCLEAIKCLSSVNAITLLRCSSLYRTEPIG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
LKNQPWFVNAVAEIRTSLSPRALLRILMQIEEAMGRVREAHWGPRIIDLDILLFGQEIVQ
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
EQDLKIPHPELHKRRFVLIPLSELASYAIHPAFGVSIRGLMERLEDDSRVERIEYG
HCCCCCCCHHHHCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10080886 [H]