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The map label for this gene is argG

Identifier: 85859274

GI number: 85859274

Start: 1461250

End: 1462455

Strand: Direct

Name: argG

Synonym: SYN_02157

Alternate gene names: 85859274

Gene position: 1461250-1462455 (Clockwise)

Preceding gene: 85859273

Following gene: 85859275

Centisome position: 45.96

GC content: 52.49

Gene sequence:

>1206_bases
GTGGATACCGGTGTAAAAAAAGTAGTTCTTGCTTATTCTGGCGGACTGGATACGTCAGTCATCGTCCAATGGTTGATCGA
AACATACGGATGCGAAGTCATTGCCTTTGCCGCTGACGTCGGTCAGAAGGAAGAACTGGAAGGATTAAGGGAAAAAGCCA
TAAAAACCGGGGCCAGTAAAATCTATATCGAGGATCTCCGCGAAGAATTCGCCCGGGATTTTGTTTTTCCGGCACTGCGG
GCAAACGCCATCTACGAGGGGACCTATCTGCTGGGAACATCGCTGGCAAGACCTTTGATTGCCAAACGCCAGGTGGAAAT
TGCCCGGGCGGAAGGGGCGGATGCGGTTTGTCATGGGGCAACGGGTAAAGGGAATGATCAGGTGCGGTTCGAATTGACCT
ACATGGCCTTGGAGCCGAACATCCGGATCATTTCGGCCTGGAAGGATCCAAACTGGCATTTCAAATCCCGGGAAGACATG
TTTGATTACGCGGAAAAGCACAATATCCCGCTTCCTCTCACGCGGGAAAAGCCTTACAGCAGCGACCGCAATCTGTTGCA
TATCTCACATGAGGGCGCCATCCTGGAAGATCCCTGGGCGGAACCGCCTGAAGACATTTTTACGATATCCTTATCTCCTG
AAGCAGCACCGGATAAGCCGACTTACGTGGAAATCGATTTCGAACAGGGAAATCCCGTTGCTCTCGACGGTGCACGTCTG
AGTCCGGCAGCCCTGATGGAGAAGATGAATGACATAGCGGGGGCCAACGGCATCGGCCGTGTCGATATGGTGGAAAACCG
GTTCGTCGGAATGAAATCCCGAGGGGTTTATGAGACGCCGGGAGGGACCGTGCTCTGGGCGGCACACCGCGCCCTGGAGT
CTCTCACCATGGACAGGGAGGTGATGCTGATGCGCGATTCTCTCATTCCCAAATACGCCCAGCTGGCTTACAATGGATTC
TGGTATGCCCCGGAAATGGAAGCCCTGCAGGCCTTGATCGATGAAACCCAGAAAAAGGCAACGGGCACGGTCCGGATGAA
ACTCTACAAGGGCAACTCTATTGTCGTCGGCAGAAAGTCCCCATATTCCCTGTATAGTGAAGACTTTGCAACCTTTGAAA
AAGATCAGGTTTACAATCAGATGGATGCAACGGGGTTCATTCGGCTCAATGCATTGAGACTGCGAATCGCGGCCATGAAG
AAATAA

Upstream 100 bases:

>100_bases
CTAGATGGCCCCCGGTCGGTCATCATCGATCAGGCGGAAAACCGTCTGCATGTTCAGAAAGCGATACTGGAAATTTTAAT
TTAGTGAAAGGAGAAGAAAA

Downstream 100 bases:

>100_bases
AAATGCGAAATCTGTCAGAAAGATAAGGGTTTTCACGAGAATGAAGGACAGCACGGAAAAGAGCAAACCCTGGGGCGGGC
GATTTCAGGAACCGACTAAC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDTGVKKVVLAYSGGLDTSVIVQWLIETYGCEVIAFAADVGQKEELEGLREKAIKTGASKIYIEDLREEFARDFVFPALR
ANAIYEGTYLLGTSLARPLIAKRQVEIARAEGADAVCHGATGKGNDQVRFELTYMALEPNIRIISAWKDPNWHFKSREDM
FDYAEKHNIPLPLTREKPYSSDRNLLHISHEGAILEDPWAEPPEDIFTISLSPEAAPDKPTYVEIDFEQGNPVALDGARL
SPAALMEKMNDIAGANGIGRVDMVENRFVGMKSRGVYETPGGTVLWAAHRALESLTMDREVMLMRDSLIPKYAQLAYNGF
WYAPEMEALQALIDETQKKATGTVRMKLYKGNSIVVGRKSPYSLYSEDFATFEKDQVYNQMDATGFIRLNALRLRIAAMK
K

Sequences:

>Translated_401_residues
MDTGVKKVVLAYSGGLDTSVIVQWLIETYGCEVIAFAADVGQKEELEGLREKAIKTGASKIYIEDLREEFARDFVFPALR
ANAIYEGTYLLGTSLARPLIAKRQVEIARAEGADAVCHGATGKGNDQVRFELTYMALEPNIRIISAWKDPNWHFKSREDM
FDYAEKHNIPLPLTREKPYSSDRNLLHISHEGAILEDPWAEPPEDIFTISLSPEAAPDKPTYVEIDFEQGNPVALDGARL
SPAALMEKMNDIAGANGIGRVDMVENRFVGMKSRGVYETPGGTVLWAAHRALESLTMDREVMLMRDSLIPKYAQLAYNGF
WYAPEMEALQALIDETQKKATGTVRMKLYKGNSIVVGRKSPYSLYSEDFATFEKDQVYNQMDATGFIRLNALRLRIAAMK
K
>Mature_401_residues
MDTGVKKVVLAYSGGLDTSVIVQWLIETYGCEVIAFAADVGQKEELEGLREKAIKTGASKIYIEDLREEFARDFVFPALR
ANAIYEGTYLLGTSLARPLIAKRQVEIARAEGADAVCHGATGKGNDQVRFELTYMALEPNIRIISAWKDPNWHFKSREDM
FDYAEKHNIPLPLTREKPYSSDRNLLHISHEGAILEDPWAEPPEDIFTISLSPEAAPDKPTYVEIDFEQGNPVALDGARL
SPAALMEKMNDIAGANGIGRVDMVENRFVGMKSRGVYETPGGTVLWAAHRALESLTMDREVMLMRDSLIPKYAQLAYNGF
WYAPEMEALQALIDETQKKATGTVRMKLYKGNSIVVGRKSPYSLYSEDFATFEKDQVYNQMDATGFIRLNALRLRIAAMK
K

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=395, Percent_Identity=49.3670886075949, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI16950633, Length=395, Percent_Identity=49.3670886075949, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI1789563, Length=401, Percent_Identity=27.1820448877805, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324514, Length=402, Percent_Identity=46.5174129353234, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI21358151, Length=399, Percent_Identity=43.609022556391, Blast_Score=347, Evalue=1e-95,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SYNAS (Q2LT97)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461476.1
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q2LT97
- SMR:   Q2LT97
- STRING:   Q2LT97
- GeneID:   3884307
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02157
- NMPDR:   fig|56780.10.peg.1414
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q2LT97
- ProtClustDB:   PRK00509
- BioCyc:   SACI56780:SYN_02157-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44907; Mature: 44907

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 89-89 BINDING 94-94 BINDING 119-119 BINDING 121-121 BINDING 125-125 BINDING 125-125 BINDING 126-126 BINDING 129-129 BINDING 180-180 BINDING 189-189 BINDING 265-265 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGVKKVVLAYSGGLDTSVIVQWLIETYGCEVIAFAADVGQKEELEGLREKAIKTGASK
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCE
IYIEDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAKRQVEIARAEGADAVCHGA
EHHHHHHHHHHHHHHHHHHHCCEEECCEEEHHHHHHHHHHHHHHHHEECCCCCCEEEECC
TGKGNDQVRFELTYMALEPNIRIISAWKDPNWHFKSREDMFDYAEKHNIPLPLTREKPYS
CCCCCCEEEEEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
SDRNLLHISHEGAILEDPWAEPPEDIFTISLSPEAAPDKPTYVEIDFEQGNPVALDGARL
CCCCEEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCC
SPAALMEKMNDIAGANGIGRVDMVENRFVGMKSRGVYETPGGTVLWAAHRALESLTMDRE
CHHHHHHHHHHHCCCCCCCCEEEHHCCCCCCCCCCCEECCCCEEEHHHHHHHHHHCCCHH
VMLMRDSLIPKYAQLAYNGFWYAPEMEALQALIDETQKKATGTVRMKLYKGNSIVVGRKS
HHHHHHHCCHHHHHHHHCCEEECCHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCC
PYSLYSEDFATFEKDQVYNQMDATGFIRLNALRLRIAAMKK
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCC
>Mature Secondary Structure
MDTGVKKVVLAYSGGLDTSVIVQWLIETYGCEVIAFAADVGQKEELEGLREKAIKTGASK
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCE
IYIEDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAKRQVEIARAEGADAVCHGA
EHHHHHHHHHHHHHHHHHHHCCEEECCEEEHHHHHHHHHHHHHHHHEECCCCCCEEEECC
TGKGNDQVRFELTYMALEPNIRIISAWKDPNWHFKSREDMFDYAEKHNIPLPLTREKPYS
CCCCCCEEEEEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
SDRNLLHISHEGAILEDPWAEPPEDIFTISLSPEAAPDKPTYVEIDFEQGNPVALDGARL
CCCCEEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCC
SPAALMEKMNDIAGANGIGRVDMVENRFVGMKSRGVYETPGGTVLWAAHRALESLTMDRE
CHHHHHHHHHHHCCCCCCCCEEEHHCCCCCCCCCCCEECCCCEEEHHHHHHHHHHCCCHH
VMLMRDSLIPKYAQLAYNGFWYAPEMEALQALIDETQKKATGTVRMKLYKGNSIVVGRKS
HHHHHHHCCHHHHHHHHCCEEECCHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCC
PYSLYSEDFATFEKDQVYNQMDATGFIRLNALRLRIAAMKK
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA