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The map label for this gene is argH

Identifier: 85859275

GI number: 85859275

Start: 1462496

End: 1463914

Strand: Direct

Name: argH

Synonym: SYN_02158

Alternate gene names: 85859275

Gene position: 1462496-1463914 (Clockwise)

Preceding gene: 85859274

Following gene: 85859276

Centisome position: 46.0

GC content: 52.36

Gene sequence:

>1419_bases
ATGAAGGACAGCACGGAAAAGAGCAAACCCTGGGGCGGGCGATTTCAGGAACCGACTAACGAACTCGTGGAGGCATTCAC
TGCTTCGATATCGTTTGATCGGCGTCTCTATCGTTATGACATCGAAGGCAGTATCGCTCATGCCCGAATGCTGGTTCGTC
AGGAAATCATAAACCGGAAAGAAGGCTTTGCCATCGTCGAGGGCCTGAAGGAGATTCAGCGGGACATTGAAACCGGCAGT
TTTGAGTTTTCTCCTGGAGATGAAGACATCCATATGGCGATTGAAAAGGATCTGATCCGACGCATCGGCGATGCGGGGGC
AAAACTGCATACCGGTAGAAGCAGAAATGATCAGGTCGCCCTGGATGTCCGCCTCTATCTTCGGGCTGAAATCAAAGAGA
TTCTTGAGTTTATGAAGGTGCTCAAAGGAGCCTTCCTTGAGCTGGCAAAGAAAGAGGCGGAAACCATCCTTCCCGGCTAT
ACCCATCTGCAGAAGGCGCAGCCGGTCCTTCTGGCTCATTATCTTCTTGCCTGGCGGGAAATGCTCGATCGTGATGAATC
ACGCCTGCGGGATTGTTACCGCCGTGTGAATGTCCTCCCTCTGGGGGCTGCTGCACTGGCCGGGACCTCTCTACCCATTG
ACCGAGCCTATGTCGCCCGGCTGCTCAAATTTCCCGAAATATCCCGCAACAGCATGGATACAGTTTCCGACCGCGATTTC
GTTGCCGAATTTCTCTTTGCCTCCTCTCTGATCATGATGCACCTGAGCCGTTTCTGTGAAGATCTGATCCTCTGGTCCTC
AGGGGAATTTAATTTTGTGGAAATCTCCGATGCCTTCACGACGGGCAGCAGCATCATGCCTCAGAAAAAGAATCCCGATG
TGGCGGAACTGATTCGGGGCAAGACGGGAAGGGTGTATGGCAATCTTATCGCTTTGTTGACCCTGCTGAAGGGACTGCCC
ATGACCTACAACCGGGACCTTCAGGAAGACAAAGAACCCCTTTTCGATACCGTCGATACGGTGAAGGCCTGCCTTCAGAT
CCTTGCGGAAATGATTCGAAACATGAAGTTCAACCGGGAAAAGATGAGGCAGGAAGCCGAGGGCGGATTCTCCACAGCGA
CGGACTTGGCTGAATATCTGGTGATGAAAGGCATTCCGTTCCGGGAAGCCCACGGCATCGTCGGGAAGCTGGTCTCTTTT
TGCATTGAATGCAAGAAGGAACTGGCGCAGTTGTCCATGGAGGAATTTCAAAGGTTCTGTCCGGTGATCAACGAGGATAT
TCATGATCGCCTTTCCGTGTCGAACTCGGTTCGGAGCAGAAAAAGCTACGGGGGAACAGCGTGTCGGCAGGTCATGGAGC
AGATCCGGCAGATAGAAGAAGGGGCTTATCCCTCAACCAGGAGAAGAAAAGAAGCATGA

Upstream 100 bases:

>100_bases
GCAACGGGGTTCATTCGGCTCAATGCATTGAGACTGCGAATCGCGGCCATGAAGAAATAAAAATGCGAAATCTGTCAGAA
AGATAAGGGTTTTCACGAGA

Downstream 100 bases:

>100_bases
AACGGTTCAGTTCATGTTTGAGGGGGCACGATAACCCGTCAGGACGTTGGATATCGGTTCTGTGGTTGATGGTTTTTATT
CCGGGTTTGATAATGGGATG

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRKEGFAIVEGLKEIQRDIETGS
FEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVALDVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGY
THLQKAQPVLLAHYLLAWREMLDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDF
VAEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRGKTGRVYGNLIALLTLLKGLP
MTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNREKMRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSF
CIECKKELAQLSMEEFQRFCPVINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQIRQIEEGAYPSTRRRKEA

Sequences:

>Translated_472_residues
MKDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRKEGFAIVEGLKEIQRDIETGS
FEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVALDVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGY
THLQKAQPVLLAHYLLAWREMLDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDF
VAEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRGKTGRVYGNLIALLTLLKGLP
MTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNREKMRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSF
CIECKKELAQLSMEEFQRFCPVINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQIRQIEEGAYPSTRRRKEA
>Mature_472_residues
MKDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRKEGFAIVEGLKEIQRDIETGS
FEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVALDVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGY
THLQKAQPVLLAHYLLAWREMLDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDF
VAEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRGKTGRVYGNLIALLTLLKGLP
MTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNREKMRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSF
CIECKKELAQLSMEEFQRFCPVINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQIRQIEEGAYPSTRRRKEA

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=456, Percent_Identity=46.0526315789474, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI68303542, Length=456, Percent_Identity=46.0526315789474, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI68303549, Length=456, Percent_Identity=43.421052631579, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI68303547, Length=456, Percent_Identity=43.2017543859649, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1790398, Length=446, Percent_Identity=46.8609865470852, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321806, Length=466, Percent_Identity=41.8454935622318, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI221473854, Length=461, Percent_Identity=41.2147505422993, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78706858, Length=461, Percent_Identity=41.2147505422993, Blast_Score=370, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_SYNAS (Q2LT96)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461477.1
- ProteinModelPortal:   Q2LT96
- SMR:   Q2LT96
- STRING:   Q2LT96
- GeneID:   3884308
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02158
- NMPDR:   fig|56780.10.peg.1415
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- PhylomeDB:   Q2LT96
- BioCyc:   SACI56780:SYN_02158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 53889; Mature: 53889

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
EGFAIVEGLKEIQRDIETGSFEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVA
CCHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEE
LDVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGYTHLQKAQPVLLAHYLLAWRE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
MLDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDF
HHCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
VAEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCCCCCCCCHHHHHCC
KTGRVYGNLIALLTLLKGLPMTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNRE
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KMRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSFCIECKKELAQLSMEEFQRFC
HHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQIRQIEEGAYPSTRRRKEA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
EGFAIVEGLKEIQRDIETGSFEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVA
CCHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEE
LDVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGYTHLQKAQPVLLAHYLLAWRE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
MLDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDF
HHCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
VAEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCCCCCCCCHHHHHCC
KTGRVYGNLIALLTLLKGLPMTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNRE
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KMRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSFCIECKKELAQLSMEEFQRFC
HHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQIRQIEEGAYPSTRRRKEA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA