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The map label for this gene is argF

Identifier: 85859273

GI number: 85859273

Start: 1460325

End: 1461233

Strand: Direct

Name: argF

Synonym: SYN_02156

Alternate gene names: 85859273

Gene position: 1460325-1461233 (Clockwise)

Preceding gene: 85859272

Following gene: 85859274

Centisome position: 45.93

GC content: 52.59

Gene sequence:

>909_bases
ATGAAAAAGGATCTGTTAAGCATCTATGATCTTGAAGCAGGAGATTTTAAGGCTATTTTTGAGAAAGCCCGGCATCTCAA
GTCGGTGCATGAGCAAGGGGTGGCCTACACTCCTCTGAAGGGGAAAACGCTGGGAATGATTTTTGACAAATCTTCAACCA
GAACGAGGATTTCTTTCGAAGTCGGCATGTATCAGCTCGGCGGTCTGGCTCTTTTTCTCAGCAATCGGGACACACAGCTC
GGACGGGGGGAAACGGTTGCCGACAGCGCCCGGATTATGTCACGCTACCTCAATGGAATCATGATTCGCACCTTCTCTCA
CCTCATCATTGAAGAATTTGCCGCCCATGCCACCATCCCGGTGATCAACGGCCTGACGGATCTGCTGCATCCCTGTCAGA
TCCTGAGCGACCTCTTTACGATCATTGAGAAAAAAGGGAGTTACGAGAGGTTGAAAATCGTTTATGTGGGCGACGGGAAC
AACATCGCCAATTCCTGGATCAACGCTGCAGCCAGGCTCCCATTCCATTTGGCGCTTTCCTGTCCGGAAGGATATGATCC
GGATTCCGGGATTCTCGACCGCGGGGTGAAGGAAGCAAAGGAGGGGGTGTCTCTCATGCGCGATCCGTATGAGGCCGTAA
GGAATGCCGACGTCGTCTATACCGATGTCTGGGCCAGCATGGGACAGGAAGCGGAACAGGAGGCGCGGGCCAGAGTCTTC
CGGCCTTATCAGATCAATGAGGCTCTGCTTTCTCCGGCAAAAAAGGACGCCATCGTCATGCATTGCCTTCCCGCGCACCG
GGGTGAAGAAATTACCGCGGCAGTTCTAGATGGCCCCCGGTCGGTCATCATCGATCAGGCGGAAAACCGTCTGCATGTTC
AGAAAGCGATACTGGAAATTTTAATTTAG

Upstream 100 bases:

>100_bases
GCACGAGCGGAAATGTTTTGCGCTTTATTCCGCCGTTGATCATAACGGAAGCGGAAGTGGATCGGGCGGTTGAAATACTG
GACAGGGCGATGGGAGAACG

Downstream 100 bases:

>100_bases
TGAAAGGAGAAGAAAAGTGGATACCGGTGTAAAAAAAGTAGTTCTTGCTTATTCTGGCGGACTGGATACGTCAGTCATCG
TCCAATGGTTGATCGAAACA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKKDLLSIYDLEAGDFKAIFEKARHLKSVHEQGVAYTPLKGKTLGMIFDKSSTRTRISFEVGMYQLGGLALFLSNRDTQL
GRGETVADSARIMSRYLNGIMIRTFSHLIIEEFAAHATIPVINGLTDLLHPCQILSDLFTIIEKKGSYERLKIVYVGDGN
NIANSWINAAARLPFHLALSCPEGYDPDSGILDRGVKEAKEGVSLMRDPYEAVRNADVVYTDVWASMGQEAEQEARARVF
RPYQINEALLSPAKKDAIVMHCLPAHRGEEITAAVLDGPRSVIIDQAENRLHVQKAILEILI

Sequences:

>Translated_302_residues
MKKDLLSIYDLEAGDFKAIFEKARHLKSVHEQGVAYTPLKGKTLGMIFDKSSTRTRISFEVGMYQLGGLALFLSNRDTQL
GRGETVADSARIMSRYLNGIMIRTFSHLIIEEFAAHATIPVINGLTDLLHPCQILSDLFTIIEKKGSYERLKIVYVGDGN
NIANSWINAAARLPFHLALSCPEGYDPDSGILDRGVKEAKEGVSLMRDPYEAVRNADVVYTDVWASMGQEAEQEARARVF
RPYQINEALLSPAKKDAIVMHCLPAHRGEEITAAVLDGPRSVIIDQAENRLHVQKAILEILI
>Mature_302_residues
MKKDLLSIYDLEAGDFKAIFEKARHLKSVHEQGVAYTPLKGKTLGMIFDKSSTRTRISFEVGMYQLGGLALFLSNRDTQL
GRGETVADSARIMSRYLNGIMIRTFSHLIIEEFAAHATIPVINGLTDLLHPCQILSDLFTIIEKKGSYERLKIVYVGDGN
NIANSWINAAARLPFHLALSCPEGYDPDSGILDRGVKEAKEGVSLMRDPYEAVRNADVVYTDVWASMGQEAEQEARARVF
RPYQINEALLSPAKKDAIVMHCLPAHRGEEITAAVLDGPRSVIIDQAENRLHVQKAILEILI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=38.1578947368421, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=28.9389067524116, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=34.1614906832298, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=34.0557275541796, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=29.2604501607717, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI48994908, Length=359, Percent_Identity=23.3983286908078, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=28.3950617283951, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=318, Percent_Identity=33.9622641509434, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=27.3615635179153, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24642586, Length=323, Percent_Identity=28.4829721362229, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_SYNAS (Q2LT98)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461475.1
- HSSP:   P96108
- ProteinModelPortal:   Q2LT98
- SMR:   Q2LT98
- STRING:   Q2LT98
- GeneID:   3884306
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02156
- NMPDR:   fig|56780.10.peg.1413
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   Q2LT98
- BioCyc:   SACI56780:SYN_02156-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33597; Mature: 33597

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 103-103 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDLLSIYDLEAGDFKAIFEKARHLKSVHEQGVAYTPLKGKTLGMIFDKSSTRTRISFE
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEECCCCCEEEEEE
VGMYQLGGLALFLSNRDTQLGRGETVADSARIMSRYLNGIMIRTFSHLIIEEFAAHATIP
ECCEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VINGLTDLLHPCQILSDLFTIIEKKGSYERLKIVYVGDGNNIANSWINAAARLPFHLALS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEE
CPEGYDPDSGILDRGVKEAKEGVSLMRDPYEAVRNADVVYTDVWASMGQEAEQEARARVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEHHHHHHCHHHHHHHHHHHC
RPYQINEALLSPAKKDAIVMHCLPAHRGEEITAAVLDGPRSVIIDQAENRLHVQKAILEI
CCCCHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCHHEEECCCCCHHHHHHHHHHH
LI
HC
>Mature Secondary Structure
MKKDLLSIYDLEAGDFKAIFEKARHLKSVHEQGVAYTPLKGKTLGMIFDKSSTRTRISFE
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEECCCCCEEEEEE
VGMYQLGGLALFLSNRDTQLGRGETVADSARIMSRYLNGIMIRTFSHLIIEEFAAHATIP
ECCEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VINGLTDLLHPCQILSDLFTIIEKKGSYERLKIVYVGDGNNIANSWINAAARLPFHLALS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEE
CPEGYDPDSGILDRGVKEAKEGVSLMRDPYEAVRNADVVYTDVWASMGQEAEQEARARVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEHHHHHHCHHHHHHHHHHHC
RPYQINEALLSPAKKDAIVMHCLPAHRGEEITAAVLDGPRSVIIDQAENRLHVQKAILEI
CCCCHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCHHEEECCCCCHHHHHHHHHHH
LI
HC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA