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The map label for this gene is moeB [H]

Identifier: 85374941

GI number: 85374941

Start: 2144032

End: 2144805

Strand: Direct

Name: moeB [H]

Synonym: ELI_10570

Alternate gene names: 85374941

Gene position: 2144032-2144805 (Clockwise)

Preceding gene: 85374940

Following gene: 228879505

Centisome position: 70.24

GC content: 66.15

Gene sequence:

>774_bases
ATGAGCCTGTCCCCCGAGAGACTGGAGCGCTTCGCCCGCCATATCGTACTGCCTGAGATTGGCGGTGCGGGACAGGCGGC
ATTGATCGACAAGCACATCGTCCTTGTGGGCCTCGGCGGCATCGGTTCGCCAGCGCTGCAATATCTTGCAGGCGCGGGGA
TCGGGCGGCTGACGCTGGTCGATAGCGACACCGTCGACGCCAGCAACCTCCAGCGCCAGACGCTCTTTTCGGAGCGCGAT
GTCGGCCACGGCAAAGCTGTATCGGCGCGTCGCTGGGTGCAGAATTTCGACCGCTCGCTGCGGGTGACCATCAGCGACAA
GCGGATCACCGCCGATAATGCGCCTGACCTGATGACCTCCGCCGATCTCGTGCTCGACGGGACGGACAACTTCGCCACAC
GCCTCGCGGTCTCCGATGCCTGCGTCGCTGCGCAAGTACCGCTCCTCACCGCCGCGGTGGGGCGTTTCCAAGGCCAGGTC
GCGGCCTTTGCCGGGCATCTGCCCAACCAGCCCTGCTACCGCTGCTTCGTCGGCGATGCCTTCGATGCCGAGGATTGCGA
CACCTGCGCCGAAGACGGCGTGCTCGGCGCAATGGTCGGCTGGGTCGGGACCTTCGCCGCCATGCAGGCCGTGCGGGTGC
TGCTGGCGGGTGTGAGCCGCTTCGGCGACCCGCAATGGGGCAAGCTGCAGTTGATGGATGGCCTCAAGCCGGGGATGCGG
GCGATTGCAATCCCGAAGGATCCGGGGTGTAAGGGATGCGGCGAGGGACGTTAA

Upstream 100 bases:

>100_bases
GTCTCGAGCAGCTATCGAGCAAGCGGCATAGCCGCGCCGTCGAGATGGCGTGTCGAGAGAGTTCTCGACAGCCAGTGCGC
ATTCCGTCAACAGCTTCTCC

Downstream 100 bases:

>100_bases
ATCCGCGGCAGGACTGTGTCCGGTCGTCGCTTCATAGCTCGCACATCGCACTTAGCATCAGGCCAAAGCTGAACCCGGTA
CTCATCTTCGCCGTCGAGAC

Product: molybdopterin biosynthesis protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSLSPERLERFARHIVLPEIGGAGQAALIDKHIVLVGLGGIGSPALQYLAGAGIGRLTLVDSDTVDASNLQRQTLFSERD
VGHGKAVSARRWVQNFDRSLRVTISDKRITADNAPDLMTSADLVLDGTDNFATRLAVSDACVAAQVPLLTAAVGRFQGQV
AAFAGHLPNQPCYRCFVGDAFDAEDCDTCAEDGVLGAMVGWVGTFAAMQAVRVLLAGVSRFGDPQWGKLQLMDGLKPGMR
AIAIPKDPGCKGCGEGR

Sequences:

>Translated_257_residues
MSLSPERLERFARHIVLPEIGGAGQAALIDKHIVLVGLGGIGSPALQYLAGAGIGRLTLVDSDTVDASNLQRQTLFSERD
VGHGKAVSARRWVQNFDRSLRVTISDKRITADNAPDLMTSADLVLDGTDNFATRLAVSDACVAAQVPLLTAAVGRFQGQV
AAFAGHLPNQPCYRCFVGDAFDAEDCDTCAEDGVLGAMVGWVGTFAAMQAVRVLLAGVSRFGDPQWGKLQLMDGLKPGMR
AIAIPKDPGCKGCGEGR
>Mature_256_residues
SLSPERLERFARHIVLPEIGGAGQAALIDKHIVLVGLGGIGSPALQYLAGAGIGRLTLVDSDTVDASNLQRQTLFSERDV
GHGKAVSARRWVQNFDRSLRVTISDKRITADNAPDLMTSADLVLDGTDNFATRLAVSDACVAAQVPLLTAAVGRFQGQVA
AFAGHLPNQPCYRCFVGDAFDAEDCDTCAEDGVLGAMVGWVGTFAAMQAVRVLLAGVSRFGDPQWGKLQLMDGLKPGMRA
IAIPKDPGCKGCGEGR

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=235, Percent_Identity=38.7234042553192, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI4885649, Length=144, Percent_Identity=34.7222222222222, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787048, Length=254, Percent_Identity=39.3700787401575, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI87082356, Length=245, Percent_Identity=38.7755102040816, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1789177, Length=122, Percent_Identity=35.2459016393443, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=248, Percent_Identity=32.258064516129, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI193203301, Length=152, Percent_Identity=32.2368421052632, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71984476, Length=114, Percent_Identity=35.0877192982456, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=215, Percent_Identity=36.7441860465116, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320598, Length=158, Percent_Identity=29.746835443038, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582879, Length=254, Percent_Identity=37.007874015748, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24660640, Length=156, Percent_Identity=33.974358974359, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 27107; Mature: 26976

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSPERLERFARHIVLPEIGGAGQAALIDKHIVLVGLGGIGSPALQYLAGAGIGRLTLV
CCCCHHHHHHHHHHCCCCCCCCCCCCHHEECEEEEEEECCCCHHHHHHHHCCCCCEEEEE
DSDTVDASNLQRQTLFSERDVGHGKAVSARRWVQNFDRSLRVTISDKRITADNAPDLMTS
ECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCC
ADLVLDGTDNFATRLAVSDACVAAQVPLLTAAVGRFQGQVAAFAGHLPNQPCYRCFVGDA
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCC
FDAEDCDTCAEDGVLGAMVGWVGTFAAMQAVRVLLAGVSRFGDPQWGKLQLMDGLKPGMR
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCE
AIAIPKDPGCKGCGEGR
EEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SLSPERLERFARHIVLPEIGGAGQAALIDKHIVLVGLGGIGSPALQYLAGAGIGRLTLV
CCCHHHHHHHHHHCCCCCCCCCCCCHHEECEEEEEEECCCCHHHHHHHHCCCCCEEEEE
DSDTVDASNLQRQTLFSERDVGHGKAVSARRWVQNFDRSLRVTISDKRITADNAPDLMTS
ECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCC
ADLVLDGTDNFATRLAVSDACVAAQVPLLTAAVGRFQGQVAAFAGHLPNQPCYRCFVGDA
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCC
FDAEDCDTCAEDGVLGAMVGWVGTFAAMQAVRVLLAGVSRFGDPQWGKLQLMDGLKPGMR
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCE
AIAIPKDPGCKGCGEGR
EEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]