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The map label for this gene is dnaA

Identifier: 85374940

GI number: 85374940

Start: 2142392

End: 2143858

Strand: Direct

Name: dnaA

Synonym: ELI_10565

Alternate gene names: 85374940

Gene position: 2142392-2143858 (Clockwise)

Preceding gene: 85374939

Following gene: 85374941

Centisome position: 70.19

GC content: 62.71

Gene sequence:

>1467_bases
ATGGCGAGGACGGATGGCCGTGCGGGAGCGGCGAAGCGCAATAAGAAAGAAGATTTGATGGAAGATCTCGAAGCGGTGAA
CCTGGCGGCGGATTGGGCAGATATCAGTCAGGGCCTGCGAAAGGATCTCGGTCACCAATTGCACAGCCAGTGGATCAAAC
CGATCCAACTCGGCGCTTTCGACGACGAGACGGGCACGCTCGACCTGTTCTTGCCGACGGAGTTTTCCGCCAACTGGGTC
AAGGATCGCTTCGCCGATCGCCTGACGCTGGCGTGGAAGATCGCCCGCGGCAATATCCGCAACGTCAACATCCAGGTCCA
TCCGGGCCGCCGCAAGGTTCCCGACATCGCGCTTGGCGACGGTCGTCGTCCGGCCAACGATGGCGGCGACATGGGGATCA
TGGTCAATGGCCCTGGCGTCATCAACGATGCCGGGTTCACTTCGTCGGTCGGCCTCGACGCTTCGCTGACATTCGCAGCC
TTCATTACGGGTGAGACCAACATCCTCGCCTGCAATGCCGCGCAGCGCATGGCCGCGACCGAGCAGCCGCAGTTCAGCCC
GCTCTACATCAAGGCCGGCACGGGTCAGGGCAAGACCCACCTGCTGCACGCGATCGGGCACCAGTATCTCGCTTCGCATC
CACGAGCGCGCATCTTCTATTGCAGCGCCGAGCGCTTCATGGTCGAGTTCGTCCAGGCGCTCAAAGCCAACCAGATGATC
GAGTTCAAGGCGCGGCTGCGCAGCTTCGACCTGCTGCTGGTGGACGATATTCAGTTCATCATCGGCAAGGCCAGCGCCCA
AGAAGAACTGCTCTACACGATCGACCACCTGCTGGCCGAAGGCAAGCGACTGGTCTTCGCCGCCGACCGGGCGCCGCAGG
CCCTGGACGGTGTCGAACCGCGCCTGCTCTCGCGCCTGTCGATGGGCCTCGTCGCCGATATCCAGGCCGCCGATATCGAG
CTTCGTAAGAAAATCCTCGAAAGCAAGCTGACGCGCTTTGCACCGCTCGACGTGCCGGAGGACGTGATCGAGTTCCTCGC
CCGCACCATCACACGTAATGTTCGCGAACTGGTCGGGGGCCTGAACAAGCTGATCGCCTATGCCCAGCTGACCGGGCAGG
TCGTATCGCTCCAGCTCGCCGAAGAGCAGCTGACCGATATCCTCTCGGCCAACCGTCGCCGCATCACCATCGACGAGATT
CAACGCACGGTGTGCCAGTTCTATCGCATCGACCGCAGCGAAATGTCGTCCAAGCGCCGCGCCCGCGCCGTCGTGCGCCC
GCGCCAGGTGGCGATGTATCTCTCCAAGGTGCTGACGCCGCGCAGCTATCCGGAAATCGGGCGCAAGTTCGGCGGCCGCG
ATCATTCGACGGTTATCCACGCCGTGCGGCTGATCGAAGACCTGCGCCAGCGCGATGCCGATATGGATGGCGATGTCCGC
AGCCTGCTGCGGCAATTGGAGAGCTGA

Upstream 100 bases:

>100_bases
AGTCCGACCGTACAGGTTTCGGGCCACGGCGAAGCCTTGCCTGTCGAAACGGGGAAGCCATGGGCCACTTGGGGAGGCCC
GACAAGAAAGCGGGAATACG

Downstream 100 bases:

>100_bases
CCTCTCGACTGGTCGTCTCGAAGGTGCTCGACGACCGCTCGAGACGAACGGTCGTTCCTAAAGTCGTCCGTTCGTCTCGA
GCAGCTATCGAGCAAGCGGC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MARTDGRAGAAKRNKKEDLMEDLEAVNLAADWADISQGLRKDLGHQLHSQWIKPIQLGAFDDETGTLDLFLPTEFSANWV
KDRFADRLTLAWKIARGNIRNVNIQVHPGRRKVPDIALGDGRRPANDGGDMGIMVNGPGVINDAGFTSSVGLDASLTFAA
FITGETNILACNAAQRMAATEQPQFSPLYIKAGTGQGKTHLLHAIGHQYLASHPRARIFYCSAERFMVEFVQALKANQMI
EFKARLRSFDLLLVDDIQFIIGKASAQEELLYTIDHLLAEGKRLVFAADRAPQALDGVEPRLLSRLSMGLVADIQAADIE
LRKKILESKLTRFAPLDVPEDVIEFLARTITRNVRELVGGLNKLIAYAQLTGQVVSLQLAEEQLTDILSANRRRITIDEI
QRTVCQFYRIDRSEMSSKRRARAVVRPRQVAMYLSKVLTPRSYPEIGRKFGGRDHSTVIHAVRLIEDLRQRDADMDGDVR
SLLRQLES

Sequences:

>Translated_488_residues
MARTDGRAGAAKRNKKEDLMEDLEAVNLAADWADISQGLRKDLGHQLHSQWIKPIQLGAFDDETGTLDLFLPTEFSANWV
KDRFADRLTLAWKIARGNIRNVNIQVHPGRRKVPDIALGDGRRPANDGGDMGIMVNGPGVINDAGFTSSVGLDASLTFAA
FITGETNILACNAAQRMAATEQPQFSPLYIKAGTGQGKTHLLHAIGHQYLASHPRARIFYCSAERFMVEFVQALKANQMI
EFKARLRSFDLLLVDDIQFIIGKASAQEELLYTIDHLLAEGKRLVFAADRAPQALDGVEPRLLSRLSMGLVADIQAADIE
LRKKILESKLTRFAPLDVPEDVIEFLARTITRNVRELVGGLNKLIAYAQLTGQVVSLQLAEEQLTDILSANRRRITIDEI
QRTVCQFYRIDRSEMSSKRRARAVVRPRQVAMYLSKVLTPRSYPEIGRKFGGRDHSTVIHAVRLIEDLRQRDADMDGDVR
SLLRQLES
>Mature_487_residues
ARTDGRAGAAKRNKKEDLMEDLEAVNLAADWADISQGLRKDLGHQLHSQWIKPIQLGAFDDETGTLDLFLPTEFSANWVK
DRFADRLTLAWKIARGNIRNVNIQVHPGRRKVPDIALGDGRRPANDGGDMGIMVNGPGVINDAGFTSSVGLDASLTFAAF
ITGETNILACNAAQRMAATEQPQFSPLYIKAGTGQGKTHLLHAIGHQYLASHPRARIFYCSAERFMVEFVQALKANQMIE
FKARLRSFDLLLVDDIQFIIGKASAQEELLYTIDHLLAEGKRLVFAADRAPQALDGVEPRLLSRLSMGLVADIQAADIEL
RKKILESKLTRFAPLDVPEDVIEFLARTITRNVRELVGGLNKLIAYAQLTGQVVSLQLAEEQLTDILSANRRRITIDEIQ
RTVCQFYRIDRSEMSSKRRARAVVRPRQVAMYLSKVLTPRSYPEIGRKFGGRDHSTVIHAVRLIEDLRQRDADMDGDVRS
LLRQLES

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=471, Percent_Identity=36.7303609341826, Blast_Score=297, Evalue=9e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 54532; Mature: 54401

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTDGRAGAAKRNKKEDLMEDLEAVNLAADWADISQGLRKDLGHQLHSQWIKPIQLGAF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
DDETGTLDLFLPTEFSANWVKDRFADRLTLAWKIARGNIRNVNIQVHPGRRKVPDIALGD
CCCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEECCCEEEEEEEECCCCCCCCCEECCC
GRRPANDGGDMGIMVNGPGVINDAGFTSSVGLDASLTFAAFITGETNILACNAAQRMAAT
CCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHC
EQPQFSPLYIKAGTGQGKTHLLHAIGHQYLASHPRARIFYCSAERFMVEFVQALKANQMI
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCHH
EFKARLRSFDLLLVDDIQFIIGKASAQEELLYTIDHLLAEGKRLVFAADRAPQALDGVEP
HHHHHHHHCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCH
RLLSRLSMGLVADIQAADIELRKKILESKLTRFAPLDVPEDVIEFLARTITRNVRELVGG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LNKLIAYAQLTGQVVSLQLAEEQLTDILSANRRRITIDEIQRTVCQFYRIDRSEMSSKRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHH
ARAVVRPRQVAMYLSKVLTPRSYPEIGRKFGGRDHSTVIHAVRLIEDLRQRDADMDGDVR
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
SLLRQLES
HHHHHHCC
>Mature Secondary Structure 
ARTDGRAGAAKRNKKEDLMEDLEAVNLAADWADISQGLRKDLGHQLHSQWIKPIQLGAF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
DDETGTLDLFLPTEFSANWVKDRFADRLTLAWKIARGNIRNVNIQVHPGRRKVPDIALGD
CCCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEECCCEEEEEEEECCCCCCCCCEECCC
GRRPANDGGDMGIMVNGPGVINDAGFTSSVGLDASLTFAAFITGETNILACNAAQRMAAT
CCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHC
EQPQFSPLYIKAGTGQGKTHLLHAIGHQYLASHPRARIFYCSAERFMVEFVQALKANQMI
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCHH
EFKARLRSFDLLLVDDIQFIIGKASAQEELLYTIDHLLAEGKRLVFAADRAPQALDGVEP
HHHHHHHHCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCH
RLLSRLSMGLVADIQAADIELRKKILESKLTRFAPLDVPEDVIEFLARTITRNVRELVGG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LNKLIAYAQLTGQVVSLQLAEEQLTDILSANRRRITIDEIQRTVCQFYRIDRSEMSSKRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHH
ARAVVRPRQVAMYLSKVLTPRSYPEIGRKFGGRDHSTVIHAVRLIEDLRQRDADMDGDVR
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
SLLRQLES
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA