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The map label for this gene is dut [H]

Identifier: 85374932

GI number: 85374932

Start: 2134258

End: 2134731

Strand: Reverse

Name: dut [H]

Synonym: ELI_10525

Alternate gene names: 85374932

Gene position: 2134731-2134258 (Counterclockwise)

Preceding gene: 85374933

Following gene: 85374926

Centisome position: 69.94

GC content: 66.88

Gene sequence:

>474_bases
GTGGCCGCAGGCGGTAGCGCGCACGAAGTCCCCGTGCAGTTGAAACGCCTGCCGAACGGCCATGGCTTGCCGCTCCCCGA
ATACGCCACCGATGGTGCGGCAGGAATGGACGTGGTCTCGGCCGAAGACATTACCCTCGCACCGGGTGCGCGCCATGCGG
TCTCGACCGGCCTCGCGATGGCGATTCCGCCGGGCTTTGAAATCCAGGTCCGTCCGCGCTCGGGCCTTGCGCTCAAGCAC
GGCATCACCGTGCCCAACACGCCGGGCACGATTGACAGCGATTATCGGGGCGAGCTGAAAGCAATCCTGATCAACCACGG
CAGCGAACCCTTCGCCATCCATCGCGGCGACCGTATCGCGCAGCTGGTCATCGCGCCGGTTACGCGCGCGACATGGCAAG
AAGTCGACGACCTCGATGCGACGGAGCGCGGGGCGGGCGGCTTCGGTTCTACCGGCGGACACGCCGAGCTCTAG

Upstream 100 bases:

>100_bases
CTGCCGGAAATGCCCAAGGATCAGGTCGCCTATGCACTGGTCGAACGGATGGCCGAGGCGCTGGAGAAAGTGCACGAGGA
TGACTGACACAAGCGCTCAA

Downstream 100 bases:

>100_bases
CGCTTCAGCCGAAAATCCCGACTGCGAGTTCGGCCCAGACCAGCAGGAAAGCGAACACTGCGCCGGCCATCAAGAGGCTG
CGAGCGCGGATGGTCTTGCC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAAGGSAHEVPVQLKRLPNGHGLPLPEYATDGAAGMDVVSAEDITLAPGARHAVSTGLAMAIPPGFEIQVRPRSGLALKH
GITVPNTPGTIDSDYRGELKAILINHGSEPFAIHRGDRIAQLVIAPVTRATWQEVDDLDATERGAGGFGSTGGHAEL

Sequences:

>Translated_157_residues
MAAGGSAHEVPVQLKRLPNGHGLPLPEYATDGAAGMDVVSAEDITLAPGARHAVSTGLAMAIPPGFEIQVRPRSGLALKH
GITVPNTPGTIDSDYRGELKAILINHGSEPFAIHRGDRIAQLVIAPVTRATWQEVDDLDATERGAGGFGSTGGHAEL
>Mature_156_residues
AAGGSAHEVPVQLKRLPNGHGLPLPEYATDGAAGMDVVSAEDITLAPGARHAVSTGLAMAIPPGFEIQVRPRSGLALKHG
ITVPNTPGTIDSDYRGELKAILINHGSEPFAIHRGDRIAQLVIAPVTRATWQEVDDLDATERGAGGFGSTGGHAEL

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=120, Percent_Identity=49.1666666666667, Blast_Score=110, Evalue=5e-25,
Organism=Homo sapiens, GI4503423, Length=122, Percent_Identity=48.3606557377049, Blast_Score=110, Evalue=6e-25,
Organism=Homo sapiens, GI70906441, Length=127, Percent_Identity=47.244094488189, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790071, Length=136, Percent_Identity=47.0588235294118, Blast_Score=118, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71988561, Length=152, Percent_Identity=47.3684210526316, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319729, Length=129, Percent_Identity=43.4108527131783, Blast_Score=104, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24583610, Length=120, Percent_Identity=43.3333333333333, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921126, Length=120, Percent_Identity=43.3333333333333, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16239; Mature: 16108

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGGSAHEVPVQLKRLPNGHGLPLPEYATDGAAGMDVVSAEDITLAPGARHAVSTGLAM
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCHHHHCCEEE
AIPPGFEIQVRPRSGLALKHGITVPNTPGTIDSDYRGELKAILINHGSEPFAIHRGDRIA
ECCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCEE
QLVIAPVTRATWQEVDDLDATERGAGGFGSTGGHAEL
EEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAGGSAHEVPVQLKRLPNGHGLPLPEYATDGAAGMDVVSAEDITLAPGARHAVSTGLAM
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCHHHHCCEEE
AIPPGFEIQVRPRSGLALKHGITVPNTPGTIDSDYRGELKAILINHGSEPFAIHRGDRIA
ECCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCEE
QLVIAPVTRATWQEVDDLDATERGAGGFGSTGGHAEL
EEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA