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The map label for this gene is intB [H]

Identifier: 85374825

GI number: 85374825

Start: 2027998

End: 2029227

Strand: Reverse

Name: intB [H]

Synonym: ELI_09990

Alternate gene names: 85374825

Gene position: 2029227-2027998 (Counterclockwise)

Preceding gene: 85374839

Following gene: 85374821

Centisome position: 66.48

GC content: 59.67

Gene sequence:

>1230_bases
ATGGTGCTAACAGACGTTGGAATTCGCAAGGCTAAGGCTGGTCCGAAAGCCTACAAACTGAGCGACTCGTCAGGGCTCTT
CCTGTTGGTGGAGCGAACCGGCGGAAAACTGTGGCGAATGAAGTATCGCATCGATGGCCGGGAGAAGAAGCTCGCGATCG
GGCGCTACCCGGAAATTGGGTTGGCAGAGGCCCGCAGAAGGCGAGACGCCGCGCGAGAGCAGCTGGCGCAGGGCAAGGAC
CCTTCCCGAGAAAAACAGCGTCAGAAGGCGCGTGCGAAGCTTGGTGCGGAAAATAATTTTGCTTCCCTGGCCGCCGAGTT
CTGCGAAAAGCGGAAACATGATGGCTCACGGGCCTGGGCACCCGCAACGGCGAAGCGCTGCGAATACCTCCTGTCAGTCC
TCAACAGCTCGATAGGCCATCTACCCATCGCCGATATTGAGCCTGCTGACATTCTGACAGCCGTCCGCCGGATCGAAAGT
AAGGGCAAGCTGGAAAGCGCTAAGCGAACCTTGCAACTGGCAGGTTCGGTGTTCCGTTATGCGGTGGCCACGGCGCGACT
CAAGTCCGATCCGACGCGCGACTTGAGGGGCGCGCTTATGAACCCCACCGTGACGCACTATGGAGCGGTGCTCGATCCAG
CAGGGGCTGGTGAGTTGCTCCGGGCTATAGATGGCTACGAAGGCCAACCTATCACCAAGCTGGCGATGCAACTGGCTCCG
CATGTGTTCGTGAGGCCCGGTGAACTCCGCCACGCCGAATGGAGCGAAATCGACTTCGATGAGGCTCTGTGGACGATCCC
GGCGAGCAAGACGAAGATGCGTAAAGACCACCTTGTCCCGCTCTCACGCCAAGCGATTGCGATCCTGGAAATGGCCCATA
GGCTGACCGGACCCGATGGCTATGTGTTCCCGTCAGTCCGGACCCGAAAGCGTCCAATGAGCGATAACACGATCAATGCT
GGACTGCGCCGCCTGGGCTACGCCACAGATGAGATGACCGCTCACGGCTTTCGCGCGATGGCGTCCACTTTGCTCAACGA
AAGCGGGAAGTGGAACCCAGACGCAATCGAGGGCGCGCTGGCGCACGGAGACAGCGACAAGGTCCGTGCAGCCTACCATC
GCGGTGCCCACTGGAAAGAACGAGTGAAGATGATGAAATGGTGGAGTGACTACCTCGACCAGCTCCGCGAAGGTGCCGAA
GTGGTGCCAATCAAGGCAGCGAAACGCTGA

Upstream 100 bases:

>100_bases
AATCTCCCGAAATTGGGGGCCTTTTTGGGGGCCTCATTGGTTTCCATCAATTCGAGAGGCCTCCAATCCGGGGGCCTTTT
CTGGGTAAAGACTTGAAATC

Downstream 100 bases:

>100_bases
CGGTTTGCGCTGAAATTCGCAACTCTTCCGCGATCCACAAAACCGCAGAGAAATATTCCGTGATTACCGTTGATTATCGC
AAAGCGAGTTGTCTGCAGCT

Product: symbiosis island integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MVLTDVGIRKAKAGPKAYKLSDSSGLFLLVERTGGKLWRMKYRIDGREKKLAIGRYPEIGLAEARRRRDAAREQLAQGKD
PSREKQRQKARAKLGAENNFASLAAEFCEKRKHDGSRAWAPATAKRCEYLLSVLNSSIGHLPIADIEPADILTAVRRIES
KGKLESAKRTLQLAGSVFRYAVATARLKSDPTRDLRGALMNPTVTHYGAVLDPAGAGELLRAIDGYEGQPITKLAMQLAP
HVFVRPGELRHAEWSEIDFDEALWTIPASKTKMRKDHLVPLSRQAIAILEMAHRLTGPDGYVFPSVRTRKRPMSDNTINA
GLRRLGYATDEMTAHGFRAMASTLLNESGKWNPDAIEGALAHGDSDKVRAAYHRGAHWKERVKMMKWWSDYLDQLREGAE
VVPIKAAKR

Sequences:

>Translated_409_residues
MVLTDVGIRKAKAGPKAYKLSDSSGLFLLVERTGGKLWRMKYRIDGREKKLAIGRYPEIGLAEARRRRDAAREQLAQGKD
PSREKQRQKARAKLGAENNFASLAAEFCEKRKHDGSRAWAPATAKRCEYLLSVLNSSIGHLPIADIEPADILTAVRRIES
KGKLESAKRTLQLAGSVFRYAVATARLKSDPTRDLRGALMNPTVTHYGAVLDPAGAGELLRAIDGYEGQPITKLAMQLAP
HVFVRPGELRHAEWSEIDFDEALWTIPASKTKMRKDHLVPLSRQAIAILEMAHRLTGPDGYVFPSVRTRKRPMSDNTINA
GLRRLGYATDEMTAHGFRAMASTLLNESGKWNPDAIEGALAHGDSDKVRAAYHRGAHWKERVKMMKWWSDYLDQLREGAE
VVPIKAAKR
>Mature_409_residues
MVLTDVGIRKAKAGPKAYKLSDSSGLFLLVERTGGKLWRMKYRIDGREKKLAIGRYPEIGLAEARRRRDAAREQLAQGKD
PSREKQRQKARAKLGAENNFASLAAEFCEKRKHDGSRAWAPATAKRCEYLLSVLNSSIGHLPIADIEPADILTAVRRIES
KGKLESAKRTLQLAGSVFRYAVATARLKSDPTRDLRGALMNPTVTHYGAVLDPAGAGELLRAIDGYEGQPITKLAMQLAP
HVFVRPGELRHAEWSEIDFDEALWTIPASKTKMRKDHLVPLSRQAIAILEMAHRLTGPDGYVFPSVRTRKRPMSDNTINA
GLRRLGYATDEMTAHGFRAMASTLLNESGKWNPDAIEGALAHGDSDKVRAAYHRGAHWKERVKMMKWWSDYLDQLREGAE
VVPIKAAKR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=394, Percent_Identity=34.2639593908629, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1788974, Length=402, Percent_Identity=32.3383084577114, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI145693166, Length=402, Percent_Identity=34.3283582089552, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45621; Mature: 45621

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLTDVGIRKAKAGPKAYKLSDSSGLFLLVERTGGKLWRMKYRIDGREKKLAIGRYPEIG
CEECCCCCHHHCCCCCEEEECCCCCEEEEEECCCCCEEEEEEEECCCHHEEEECCCCCCC
LAEARRRRDAAREQLAQGKDPSREKQRQKARAKLGAENNFASLAAEFCEKRKHDGSRAWA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
PATAKRCEYLLSVLNSSIGHLPIADIEPADILTAVRRIESKGKLESAKRTLQLAGSVFRY
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVATARLKSDPTRDLRGALMNPTVTHYGAVLDPAGAGELLRAIDGYEGQPITKLAMQLAP
HHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
HVFVRPGELRHAEWSEIDFDEALWTIPASKTKMRKDHLVPLSRQAIAILEMAHRLTGPDG
CEEECCCCCCCCCCCCCCHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
YVFPSVRTRKRPMSDNTINAGLRRLGYATDEMTAHGFRAMASTLLNESGKWNPDAIEGAL
EECCCCHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AHGDSDKVRAAYHRGAHWKERVKMMKWWSDYLDQLREGAEVVPIKAAKR
HCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MVLTDVGIRKAKAGPKAYKLSDSSGLFLLVERTGGKLWRMKYRIDGREKKLAIGRYPEIG
CEECCCCCHHHCCCCCEEEECCCCCEEEEEECCCCCEEEEEEEECCCHHEEEECCCCCCC
LAEARRRRDAAREQLAQGKDPSREKQRQKARAKLGAENNFASLAAEFCEKRKHDGSRAWA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
PATAKRCEYLLSVLNSSIGHLPIADIEPADILTAVRRIESKGKLESAKRTLQLAGSVFRY
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVATARLKSDPTRDLRGALMNPTVTHYGAVLDPAGAGELLRAIDGYEGQPITKLAMQLAP
HHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
HVFVRPGELRHAEWSEIDFDEALWTIPASKTKMRKDHLVPLSRQAIAILEMAHRLTGPDG
CEEECCCCCCCCCCCCCCHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
YVFPSVRTRKRPMSDNTINAGLRRLGYATDEMTAHGFRAMASTLLNESGKWNPDAIEGAL
EECCCCHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AHGDSDKVRAAYHRGAHWKERVKMMKWWSDYLDQLREGAEVVPIKAAKR
HCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]