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The map label for this gene is yogA [H]

Identifier: 85374291

GI number: 85374291

Start: 1521304

End: 1522314

Strand: Direct

Name: yogA [H]

Synonym: ELI_07320

Alternate gene names: 85374291

Gene position: 1521304-1522314 (Clockwise)

Preceding gene: 85374290

Following gene: 85374297

Centisome position: 49.84

GC content: 64.19

Gene sequence:

>1011_bases
ATGAAAGCCATCCGCACAGGCGCCACGCCCTCCACCCTCGACGCACTCGAACTGGTCGATATCGATGACGCACCCGCACC
CGGCCCGGGCGAAATCACGGTAAGAATCCGCGCCAGCTCGCTGAATTATCATGACTACGCTGTCGTCAAAGGCATGATCA
CGACCGAGCAGGGCCGCATCCCGATGTCGGATGGCGCAGGCGAGGTGACTGCAGTCGGCGATGGCGTAAGCGAGTTCGCT
GTCGGCGATACGGTGGTTTCGACCTTCTTCCCCCAATGGCTCGACGGCGCGCCGCCGACCAGCGCCTTCACGCAGGTACC
GGGCGACGGCATCGACGGCTATTCCCGCGAAAGCATCACCGCGCCGACCACATGGTTCACCCGCGCACCCACGGGCTACA
GCGCGGCGCAAGCGGCCACGCTTACCTGCGCCGGCCTGACCGCATGGCGGGCGCTCTTCGTCGATTACACACTCAAGCCC
GGCGACACGGTCTTGGTACAGGGCACCGGCGGCGTCTCGATCTTCGCGCTGCAACTGGCCAAGGCAGCGGGCGCAACGGT
GATCGCCACCAGCTCCTCCGACGAGAAGCTCGAACGCGTGAAGGCGCTCGGAGCCGATCACACGATCAACTACAAGGAAG
TAGAGGCCTGGGGTCCGAAAGCGCTGGAGATCACCGGCGGTCGCGGGGTCGATTGTGTCGTCGAGGTTGGCGGCGCAGGC
ACGCTGGACCAGTCCATGCTAGCCGCGCGCATCGGCGGGCATGTGGCGCTGATCGGCGTATTGTCAGGCTTCGCCGGTCC
GGTGCAGACCGCGCTGCTGTTCTCCAAGAATCTCACCGTCCAGGGCCTCACCGTAGGCAGCCGCGCGATGCAGCAGGATA
TGATTTCCGCCATAGAAGCCAACGGCATCGAGCCGGTAATCTCCGATCACTTCGCCTTGGCCGACCTGGCCGATGCTTTC
CGTCACCAGGAAGCGAACAAGCACTTCGGCAAAATCTGCGTCGAGATCTAG

Upstream 100 bases:

>100_bases
GACCGAAGGCCGCACCGAGAGTCTGATGGAGCACCGCCACCGCGCGCAGCATCACGGTTTCTTCCACTACGCCATCAAAC
CATCCAAGGACTGACCCGCC

Downstream 100 bases:

>100_bases
GGCTCGATCACGATCCCGACCGCCGGGTCGACCTTGCCGCCGAGCATTTCGAGATAGGTATCGCGCGCTGCATCGAGCCC
ATTTCGGCGTTCGATACTGA

Product: alcohol dehydrogenase, zinc-containing

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKAIRTGATPSTLDALELVDIDDAPAPGPGEITVRIRASSLNYHDYAVVKGMITTEQGRIPMSDGAGEVTAVGDGVSEFA
VGDTVVSTFFPQWLDGAPPTSAFTQVPGDGIDGYSRESITAPTTWFTRAPTGYSAAQAATLTCAGLTAWRALFVDYTLKP
GDTVLVQGTGGVSIFALQLAKAAGATVIATSSSDEKLERVKALGADHTINYKEVEAWGPKALEITGGRGVDCVVEVGGAG
TLDQSMLAARIGGHVALIGVLSGFAGPVQTALLFSKNLTVQGLTVGSRAMQQDMISAIEANGIEPVISDHFALADLADAF
RHQEANKHFGKICVEI

Sequences:

>Translated_336_residues
MKAIRTGATPSTLDALELVDIDDAPAPGPGEITVRIRASSLNYHDYAVVKGMITTEQGRIPMSDGAGEVTAVGDGVSEFA
VGDTVVSTFFPQWLDGAPPTSAFTQVPGDGIDGYSRESITAPTTWFTRAPTGYSAAQAATLTCAGLTAWRALFVDYTLKP
GDTVLVQGTGGVSIFALQLAKAAGATVIATSSSDEKLERVKALGADHTINYKEVEAWGPKALEITGGRGVDCVVEVGGAG
TLDQSMLAARIGGHVALIGVLSGFAGPVQTALLFSKNLTVQGLTVGSRAMQQDMISAIEANGIEPVISDHFALADLADAF
RHQEANKHFGKICVEI
>Mature_336_residues
MKAIRTGATPSTLDALELVDIDDAPAPGPGEITVRIRASSLNYHDYAVVKGMITTEQGRIPMSDGAGEVTAVGDGVSEFA
VGDTVVSTFFPQWLDGAPPTSAFTQVPGDGIDGYSRESITAPTTWFTRAPTGYSAAQAATLTCAGLTAWRALFVDYTLKP
GDTVLVQGTGGVSIFALQLAKAAGATVIATSSSDEKLERVKALGADHTINYKEVEAWGPKALEITGGRGVDCVVEVGGAG
TLDQSMLAARIGGHVALIGVLSGFAGPVQTALLFSKNLTVQGLTVGSRAMQQDMISAIEANGIEPVISDHFALADLADAF
RHQEANKHFGKICVEI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=330, Percent_Identity=27.5757575757576, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI22538444, Length=330, Percent_Identity=27.5757575757576, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI18379349, Length=342, Percent_Identity=25.4385964912281, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI194239674, Length=319, Percent_Identity=25.3918495297806, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI13236495, Length=319, Percent_Identity=25.3918495297806, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI28557745, Length=370, Percent_Identity=28.9189189189189, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI194239676, Length=219, Percent_Identity=26.9406392694064, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI47519420, Length=349, Percent_Identity=24.9283667621776, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790485, Length=271, Percent_Identity=29.8892988929889, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081918, Length=318, Percent_Identity=27.3584905660377, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789651, Length=279, Percent_Identity=26.8817204301075, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=334, Percent_Identity=27.5449101796407, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=337, Percent_Identity=26.4094955489614, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=327, Percent_Identity=25.3822629969419, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=335, Percent_Identity=23.8805970149254, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=341, Percent_Identity=24.633431085044, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=336, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=341, Percent_Identity=24.633431085044, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581345, Length=221, Percent_Identity=27.6018099547511, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI221330659, Length=222, Percent_Identity=27.9279279279279, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19920632, Length=222, Percent_Identity=27.9279279279279, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34868; Mature: 34868

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIRTGATPSTLDALELVDIDDAPAPGPGEITVRIRASSLNYHDYAVVKGMITTEQGRI
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCC
PMSDGAGEVTAVGDGVSEFAVGDTVVSTFFPQWLDGAPPTSAFTQVPGDGIDGYSRESIT
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
APTTWFTRAPTGYSAAQAATLTCAGLTAWRALFVDYTLKPGDTVLVQGTGGVSIFALQLA
CCCEEEEECCCCCCHHHHHEEEHHHHHHHHHHHEEEEECCCCEEEEECCCCCEEHEEEHH
KAAGATVIATSSSDEKLERVKALGADHTINYKEVEAWGPKALEITGGRGVDCVVEVGGAG
HHCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCEEEEEECCCC
TLDQSMLAARIGGHVALIGVLSGFAGPVQTALLFSKNLTVQGLTVGSRAMQQDMISAIEA
CHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHC
NGIEPVISDHFALADLADAFRHQEANKHFGKICVEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKAIRTGATPSTLDALELVDIDDAPAPGPGEITVRIRASSLNYHDYAVVKGMITTEQGRI
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCC
PMSDGAGEVTAVGDGVSEFAVGDTVVSTFFPQWLDGAPPTSAFTQVPGDGIDGYSRESIT
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
APTTWFTRAPTGYSAAQAATLTCAGLTAWRALFVDYTLKPGDTVLVQGTGGVSIFALQLA
CCCEEEEECCCCCCHHHHHEEEHHHHHHHHHHHEEEEECCCCEEEEECCCCCEEHEEEHH
KAAGATVIATSSSDEKLERVKALGADHTINYKEVEAWGPKALEITGGRGVDCVVEVGGAG
HHCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCEEEEEECCCC
TLDQSMLAARIGGHVALIGVLSGFAGPVQTALLFSKNLTVQGLTVGSRAMQQDMISAIEA
CHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHC
NGIEPVISDHFALADLADAFRHQEANKHFGKICVEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]