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The map label for this gene is ephD [H]

Identifier: 85374290

GI number: 85374290

Start: 1520467

End: 1521297

Strand: Direct

Name: ephD [H]

Synonym: ELI_07315

Alternate gene names: 85374290

Gene position: 1520467-1521297 (Clockwise)

Preceding gene: 85374289

Following gene: 85374291

Centisome position: 49.81

GC content: 66.19

Gene sequence:

>831_bases
ATGAGCGGTTACGCGCCCCGCAAGGGGGATTGGGCCGTCATCACCGGAGCCGGACGCGGAACCGGACGTTTCCTGGCGCA
GCATTTCGCGGCCAAGGGCATGCGCATCTGCGCGCTCAACATCGATGCTTACGAAGCCGAGCAGACGGCGCGGCTCTGCG
GCGAGGACAGTCGCGGGCGCGGCTGCGATGTATCGGATCGCGACGAAACGATGGAGGTCGCGGAAAAGCTTATCGCGCAG
GGGGTCGAACCCGCGCTGCTTTGGATCAATGCCGGGGTCGGCAGCGCGGATACGGTCAGCACGGTCAAGCCGCATACGCT
CGACTGGCTGATGGGGGTGAACGTACTCGGACCGGTGCATACAGCGCAGTCCTGGCTCCCAAGCCTCAAGAAGCAGGTCG
GGCCCAAACATGTCGGCATCACCGCGTCGTCCGCCTCGGTTGTGCCGGTCAGCGGACCGTTCACGCTCTACGCCACAACC
AAGCAGATGACCGCAGCGGTCGGCGAAGCGCTCGCGGCAGAACTTGCCGAGGAGCAGATCGGTGTCACGATCCTTTGCCC
CGGTATCCTCAACACGCAAATCTGGAACGCCGCGCAAGCCCGGCCCGAGCGGTTCGGCGGCGCCCGATCCGCCCCCGACG
AAGCAGGCGAACACTGGCGCGCCCAGCCCGGCCCTGAAGTCCTCGCCGAAGGGATCGACACGGTGCTCGGGCGCGGCGGT
GGCTGGTGCATCGTGCCGACCGAAGGCCGCACCGAGAGTCTGATGGAGCACCGCCACCGCGCGCAGCATCACGGTTTCTT
CCACTACGCCATCAAACCATCCAAGGACTGA

Upstream 100 bases:

>100_bases
AGAATGGCACCGTGACCTTCCGCGACGGCGAATGGACCGGCGCAACGCCGGGTGGCCTGATCCGCGGGCCGCAGCGTGCC
GAGATGCTCGAAGCCGCCGA

Downstream 100 bases:

>100_bases
CCCGCCATGAAAGCCATCCGCACAGGCGCCACGCCCTCCACCCTCGACGCACTCGAACTGGTCGATATCGATGACGCACC
CGCACCCGGCCCGGGCGAAA

Product: putative short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSGYAPRKGDWAVITGAGRGTGRFLAQHFAAKGMRICALNIDAYEAEQTARLCGEDSRGRGCDVSDRDETMEVAEKLIAQ
GVEPALLWINAGVGSADTVSTVKPHTLDWLMGVNVLGPVHTAQSWLPSLKKQVGPKHVGITASSASVVPVSGPFTLYATT
KQMTAAVGEALAAELAEEQIGVTILCPGILNTQIWNAAQARPERFGGARSAPDEAGEHWRAQPGPEVLAEGIDTVLGRGG
GWCIVPTEGRTESLMEHRHRAQHHGFFHYAIKPSKD

Sequences:

>Translated_276_residues
MSGYAPRKGDWAVITGAGRGTGRFLAQHFAAKGMRICALNIDAYEAEQTARLCGEDSRGRGCDVSDRDETMEVAEKLIAQ
GVEPALLWINAGVGSADTVSTVKPHTLDWLMGVNVLGPVHTAQSWLPSLKKQVGPKHVGITASSASVVPVSGPFTLYATT
KQMTAAVGEALAAELAEEQIGVTILCPGILNTQIWNAAQARPERFGGARSAPDEAGEHWRAQPGPEVLAEGIDTVLGRGG
GWCIVPTEGRTESLMEHRHRAQHHGFFHYAIKPSKD
>Mature_275_residues
SGYAPRKGDWAVITGAGRGTGRFLAQHFAAKGMRICALNIDAYEAEQTARLCGEDSRGRGCDVSDRDETMEVAEKLIAQG
VEPALLWINAGVGSADTVSTVKPHTLDWLMGVNVLGPVHTAQSWLPSLKKQVGPKHVGITASSASVVPVSGPFTLYATTK
QMTAAVGEALAAELAEEQIGVTILCPGILNTQIWNAAQARPERFGGARSAPDEAGEHWRAQPGPEVLAEGIDTVLGRGGG
WCIVPTEGRTESLMEHRHRAQHHGFFHYAIKPSKD

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=192, Percent_Identity=33.8541666666667, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=204, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281364892, Length=192, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 29425; Mature: 29293

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYAPRKGDWAVITGAGRGTGRFLAQHFAAKGMRICALNIDAYEAEQTARLCGEDSRGR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCC
GCDVSDRDETMEVAEKLIAQGVEPALLWINAGVGSADTVSTVKPHTLDWLMGVNVLGPVH
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCHHHCCHH
TAQSWLPSLKKQVGPKHVGITASSASVVPVSGPFTLYATTKQMTAAVGEALAAELAEEQI
HHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHC
GVTILCPGILNTQIWNAAQARPERFGGARSAPDEAGEHWRAQPGPEVLAEGIDTVLGRGG
CEEEEECCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCC
GWCIVPTEGRTESLMEHRHRAQHHGFFHYAIKPSKD
CEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCC
>Mature Secondary Structure 
SGYAPRKGDWAVITGAGRGTGRFLAQHFAAKGMRICALNIDAYEAEQTARLCGEDSRGR
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCCC
GCDVSDRDETMEVAEKLIAQGVEPALLWINAGVGSADTVSTVKPHTLDWLMGVNVLGPVH
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCHHHCCHH
TAQSWLPSLKKQVGPKHVGITASSASVVPVSGPFTLYATTKQMTAAVGEALAAELAEEQI
HHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHC
GVTILCPGILNTQIWNAAQARPERFGGARSAPDEAGEHWRAQPGPEVLAEGIDTVLGRGG
CEEEEECCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCC
GWCIVPTEGRTESLMEHRHRAQHHGFFHYAIKPSKD
CEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]