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The map label for this gene is phaA [H]

Identifier: 85374285

GI number: 85374285

Start: 1513596

End: 1515281

Strand: Direct

Name: phaA [H]

Synonym: ELI_07290

Alternate gene names: 85374285

Gene position: 1513596-1515281 (Clockwise)

Preceding gene: 85374284

Following gene: 85374286

Centisome position: 49.59

GC content: 59.37

Gene sequence:

>1686_bases
ATGGCCGTAGAATTCGATGACGAAGCCGCCCAGCACACTACCGCGCTCGGCCCCCTCGTGGGCCTGACCCGCGAGGATAT
TTTCGGCGCCGTTGCCGTACTTCTCCGCGAAACCGCTTCCGACCCGCAGCGCCTGATGCGCCACGGGCAGGAAATGGGCA
GCGACATGATCAAGATCATGACCGGCCAGAGCGAGCTTGCCCCCAATCCCAAGGACAAGCGCTTCATGGACCCGGCTTGG
CGCTACAACCCGTTCATGCGCGCCGGGATGCAATACTACCTCGCAGTGCAGAAAGGCATGTCGAGCTGGCTGGAAGATCT
TGAGCTCGACGAGCTCGAGAAGGATCGCGCGCGCTTCATCTCCAACATCATCATCGACGGACTCGCCCCCACCAACACGC
TGATGGGCAACCCGACCGCGCAGAAGCGGCTGATCGACAGCGGCGGTCTTTCGCTGATCAAGGGCCTCAAGAACGCCTAT
ACCGATATCACCGAGAACAAGGGGATGGTCAGCCAGGTCGACAAGCGACCCTTCAAGCTGGGCGAGAATGTCGCGACCTC
GAAAGGCTCGGTCGTCCTGCGCACCGAGATGATGGAACTGGTCCATTACGCGCCGACAACGGACGAAGTGTACGAGATTC
CACAGCTTACGATCCCGCCACAGATCAACAAGATGTATATCAACGACCTCTCGCCGGAGAAGTCGGTGGTGAAATACCAG
CTCGACAACGGCATCCAGACCTTCGTCATTTCCTGGCGCAATCCGTCCAAGGAACAGGGTGTGTGGGACATGGCAGATTA
TGTCGCCTCTTGCCGTGAAGCGATGGAAGCGGTGAGCGCGATCACCGGATCGAAGAAGGTCAATGTCTCGGCCGGTTGTT
CGGGCGGCCAGACCGCAGCGATGCTCGCGTCCAAGATGGCCAGCGACAAGGACGACCTGCTCGGTGCGCTGACGCTGATG
GTCTGTGTGTTGCACCCCAAGCAGAACGATATCGAGGCCGGCTCGCTGATCAGCAAGAACGGGCTCGAACTCGCCCGCCA
GCGCGCGGCGAAGAAAGGCGTGATCAAGGGCGACGATCTGGCGCGCGGTTTCGCGTGGCTGCGGCCCAACGATTTGATCT
GGAACTACGTCATCAACAATTACCTGCTGGGCGACGATCCGCCGGCTTTCGACGTCTTGTTCTGGAATGCCGATGCGACC
AATCTGTCCGCCAGCCTGATGGGCGATTTCCTGACCATTTTCGAAACGCTGGCCTTCACGAAGAAGGGCGAAGTCGAGAT
GGTCGATCACAAGATCGATCTCAGCAAAGTGAAGAGCGACCTGTTCATCCTCGGCGGTGTGACCGATCACATCACCCCGT
GGAAGGCGACCTATCGTTCGACACAGCTGTTCGGCTCAAAGGATGTCACTTACGTGCTGAGCCACTCGGGTCACATGCAG
GCGATCCTGAACCCTCCCGGCAATCCCAAAGCGCGCTATTATCTCAACAAAGGAAAGAAGCTGCCCGAGACCGCCGACGA
ATGGCAGGCTGGCGCGGAAGAAGTCGCCGGAAGCTGGTGGCCTTACTGGATGGAATGGGTGCAGAAGCGCTCCGGCGAGA
AGAAGGACGCGCCCAAGGAAGTTGGGAATTCCGACTACAAAGCGCTGGACCCGGCGCCCGGTCTTTACGTAGTGGAAAAG
GTCTGA

Upstream 100 bases:

>100_bases
CATGGCCAAGCACACTTGATTCTTGCTAAGGGCTGCATCCAGAGCAATCGGATCACGCGGCTGCGAGCCCCGCCGCGCAA
GACCAAGGAGAGCCTTGAAC

Downstream 100 bases:

>100_bases
AGACGCCTACCGGTCGCAAGGCGTCATAAGGGGACTGCGCTTTGCCAAAAAAACAGGAACTTACCGCCGATATCTCGATG
GAAGAGGTCGGTGGACGCAC

Product: PHA-synthase 1

Products: NA

Alternate protein names: PHA polymerase 1; PHA synthase 1; Polyhydroxyalkanoic acid synthase 1 [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MAVEFDDEAAQHTTALGPLVGLTREDIFGAVAVLLRETASDPQRLMRHGQEMGSDMIKIMTGQSELAPNPKDKRFMDPAW
RYNPFMRAGMQYYLAVQKGMSSWLEDLELDELEKDRARFISNIIIDGLAPTNTLMGNPTAQKRLIDSGGLSLIKGLKNAY
TDITENKGMVSQVDKRPFKLGENVATSKGSVVLRTEMMELVHYAPTTDEVYEIPQLTIPPQINKMYINDLSPEKSVVKYQ
LDNGIQTFVISWRNPSKEQGVWDMADYVASCREAMEAVSAITGSKKVNVSAGCSGGQTAAMLASKMASDKDDLLGALTLM
VCVLHPKQNDIEAGSLISKNGLELARQRAAKKGVIKGDDLARGFAWLRPNDLIWNYVINNYLLGDDPPAFDVLFWNADAT
NLSASLMGDFLTIFETLAFTKKGEVEMVDHKIDLSKVKSDLFILGGVTDHITPWKATYRSTQLFGSKDVTYVLSHSGHMQ
AILNPPGNPKARYYLNKGKKLPETADEWQAGAEEVAGSWWPYWMEWVQKRSGEKKDAPKEVGNSDYKALDPAPGLYVVEK
V

Sequences:

>Translated_561_residues
MAVEFDDEAAQHTTALGPLVGLTREDIFGAVAVLLRETASDPQRLMRHGQEMGSDMIKIMTGQSELAPNPKDKRFMDPAW
RYNPFMRAGMQYYLAVQKGMSSWLEDLELDELEKDRARFISNIIIDGLAPTNTLMGNPTAQKRLIDSGGLSLIKGLKNAY
TDITENKGMVSQVDKRPFKLGENVATSKGSVVLRTEMMELVHYAPTTDEVYEIPQLTIPPQINKMYINDLSPEKSVVKYQ
LDNGIQTFVISWRNPSKEQGVWDMADYVASCREAMEAVSAITGSKKVNVSAGCSGGQTAAMLASKMASDKDDLLGALTLM
VCVLHPKQNDIEAGSLISKNGLELARQRAAKKGVIKGDDLARGFAWLRPNDLIWNYVINNYLLGDDPPAFDVLFWNADAT
NLSASLMGDFLTIFETLAFTKKGEVEMVDHKIDLSKVKSDLFILGGVTDHITPWKATYRSTQLFGSKDVTYVLSHSGHMQ
AILNPPGNPKARYYLNKGKKLPETADEWQAGAEEVAGSWWPYWMEWVQKRSGEKKDAPKEVGNSDYKALDPAPGLYVVEK
V
>Mature_560_residues
AVEFDDEAAQHTTALGPLVGLTREDIFGAVAVLLRETASDPQRLMRHGQEMGSDMIKIMTGQSELAPNPKDKRFMDPAWR
YNPFMRAGMQYYLAVQKGMSSWLEDLELDELEKDRARFISNIIIDGLAPTNTLMGNPTAQKRLIDSGGLSLIKGLKNAYT
DITENKGMVSQVDKRPFKLGENVATSKGSVVLRTEMMELVHYAPTTDEVYEIPQLTIPPQINKMYINDLSPEKSVVKYQL
DNGIQTFVISWRNPSKEQGVWDMADYVASCREAMEAVSAITGSKKVNVSAGCSGGQTAAMLASKMASDKDDLLGALTLMV
CVLHPKQNDIEAGSLISKNGLELARQRAAKKGVIKGDDLARGFAWLRPNDLIWNYVINNYLLGDDPPAFDVLFWNADATN
LSASLMGDFLTIFETLAFTKKGEVEMVDHKIDLSKVKSDLFILGGVTDHITPWKATYRSTQLFGSKDVTYVLSHSGHMQA
ILNPPGNPKARYYLNKGKKLPETADEWQAGAEEVAGSWWPYWMEWVQKRSGEKKDAPKEVGNSDYKALDPAPGLYVVEKV

Specific function: P.oleovorans accumulates poly(3-hydroxyalkanoates) after growth on medium chain length hydrocarbons. Large amounts of this polyester are synthesized when cells are grown under nitrogen- limiting conditions. When nitrogen is resupplied in the medium, the a

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011287
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 62250; Mature: 62119

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEFDDEAAQHTTALGPLVGLTREDIFGAVAVLLRETASDPQRLMRHGQEMGSDMIKIM
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEHC
TGQSELAPNPKDKRFMDPAWRYNPFMRAGMQYYLAVQKGMSSWLEDLELDELEKDRARFI
CCCHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SNIIIDGLAPTNTLMGNPTAQKRLIDSGGLSLIKGLKNAYTDITENKGMVSQVDKRPFKL
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHC
GENVATSKGSVVLRTEMMELVHYAPTTDEVYEIPQLTIPPQINKMYINDLSPEKSVVKYQ
CCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCHHHEEEE
LDNGIQTFVISWRNPSKEQGVWDMADYVASCREAMEAVSAITGSKKVNVSAGCSGGQTAA
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
MLASKMASDKDDLLGALTLMVCVLHPKQNDIEAGSLISKNGLELARQRAAKKGVIKGDDL
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCHH
ARGFAWLRPNDLIWNYVINNYLLGDDPPAFDVLFWNADATNLSASLMGDFLTIFETLAFT
HHCEEEECCCHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
KKGEVEMVDHKIDLSKVKSDLFILGGVTDHITPWKATYRSTQLFGSKDVTYVLSHSGHMQ
CCCCEEEECCCCCHHHHCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEE
AILNPPGNPKARYYLNKGKKLPETADEWQAGAEEVAGSWWPYWMEWVQKRSGEKKDAPKE
EEECCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCCCHHH
VGNSDYKALDPAPGLYVVEKV
CCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
AVEFDDEAAQHTTALGPLVGLTREDIFGAVAVLLRETASDPQRLMRHGQEMGSDMIKIM
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEHC
TGQSELAPNPKDKRFMDPAWRYNPFMRAGMQYYLAVQKGMSSWLEDLELDELEKDRARFI
CCCHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SNIIIDGLAPTNTLMGNPTAQKRLIDSGGLSLIKGLKNAYTDITENKGMVSQVDKRPFKL
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHC
GENVATSKGSVVLRTEMMELVHYAPTTDEVYEIPQLTIPPQINKMYINDLSPEKSVVKYQ
CCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCHHHEEEE
LDNGIQTFVISWRNPSKEQGVWDMADYVASCREAMEAVSAITGSKKVNVSAGCSGGQTAA
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
MLASKMASDKDDLLGALTLMVCVLHPKQNDIEAGSLISKNGLELARQRAAKKGVIKGDDL
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCHH
ARGFAWLRPNDLIWNYVINNYLLGDDPPAFDVLFWNADATNLSASLMGDFLTIFETLAFT
HHCEEEECCCHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
KKGEVEMVDHKIDLSKVKSDLFILGGVTDHITPWKATYRSTQLFGSKDVTYVLSHSGHMQ
CCCCEEEECCCCCHHHHCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEE
AILNPPGNPKARYYLNKGKKLPETADEWQAGAEEVAGSWWPYWMEWVQKRSGEKKDAPKE
EEECCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCCCHHH
VGNSDYKALDPAPGLYVVEKV
CCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]