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The map label for this gene is phaB [H]

Identifier: 85374286

GI number: 85374286

Start: 1515359

End: 1516171

Strand: Direct

Name: phaB [H]

Synonym: ELI_07295

Alternate gene names: 85374286

Gene position: 1515359-1516171 (Clockwise)

Preceding gene: 85374285

Following gene: 85374287

Centisome position: 49.64

GC content: 62.24

Gene sequence:

>813_bases
ATGGAAGAGGTCGGTGGACGCACTTTGCGGGTGGCCAAATGGCGGCTCGATGCGCCATCGGACCACCCGCCGATCCTGTT
TTTCAACGGCATAGGCGCCAATATCGAAGCGGTCGCGCCGCTTGCCGAAGTGCTCGACGATCGCGCGTTTATCATGTTCG
ACATGCCCGGCGTCGGTGAATCGCCCGACCCGGTGCTGCCCTACAACCCTTTCACGATGAGCTGGACGGCGGCGCAGCTG
CTCGGCCGCTACGATATCGATGAAGTGGATGTGCTCGGCATCAGCTGGGGCGGCGCGATGGCGCAGCATTTCGCGCTGCA
GCACGGCGCGCGCACCCGGCGACTGGTGCTGATCGCGACCACGCCGGGCATGGCAATGGTTCCGGGTAATCCGGCGGCCT
TCACCAAGATGGCTAATCCGCGTCGCTACATCGATCCCGACTTCATGAACGAGCATTTCGCAACGCTCTACGGAGGTGCC
ACAGAAAATCCGGGTCAAAAGGCTGAGCATGTGGGCAGGCTCAAGCCTCCTTCGCCGCGCGGCTATCTGTACCAGCTGCT
GTGCATGCTCGGCTGGACCAGCCTTCCCGCCCTGCCCTTCCTCGGCAAGGAAACGCTGGTGATGATGGGCGATGACGACC
AGATCGTGCCTGTCATCAACGGCAAGATCCTCGCCCGCATGATACCGAACGCGCGGCTCGAAGTGGTCGAGGGTGGCGGA
CATCTGTTCCTGCTGACCCATGCCGACGAGAGCATCGCCCTGATCCGCGATTTTCTCGATGCTCCAGAAAACGCGGAGTC
GAAAGCCGCATAG

Upstream 100 bases:

>100_bases
TTTACGTAGTGGAAAAGGTCTGAAGACGCCTACCGGTCGCAAGGCGTCATAAGGGGACTGCGCTTTGCCAAAAAAACAGG
AACTTACCGCCGATATCTCG

Downstream 100 bases:

>100_bases
CTCGCGCCGCTGGACAGCATTCGCACAATATGAGACCTCTCCTCTCGGAGAGGGCGAACGGACACGATGGCAACCGCCGG
ACCGCGCTCGGCAACATGAG

Product: Poly(3-hydroxyalkanoate) depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MEEVGGRTLRVAKWRLDAPSDHPPILFFNGIGANIEAVAPLAEVLDDRAFIMFDMPGVGESPDPVLPYNPFTMSWTAAQL
LGRYDIDEVDVLGISWGGAMAQHFALQHGARTRRLVLIATTPGMAMVPGNPAAFTKMANPRRYIDPDFMNEHFATLYGGA
TENPGQKAEHVGRLKPPSPRGYLYQLLCMLGWTSLPALPFLGKETLVMMGDDDQIVPVINGKILARMIPNARLEVVEGGG
HLFLLTHADESIALIRDFLDAPENAESKAA

Sequences:

>Translated_270_residues
MEEVGGRTLRVAKWRLDAPSDHPPILFFNGIGANIEAVAPLAEVLDDRAFIMFDMPGVGESPDPVLPYNPFTMSWTAAQL
LGRYDIDEVDVLGISWGGAMAQHFALQHGARTRRLVLIATTPGMAMVPGNPAAFTKMANPRRYIDPDFMNEHFATLYGGA
TENPGQKAEHVGRLKPPSPRGYLYQLLCMLGWTSLPALPFLGKETLVMMGDDDQIVPVINGKILARMIPNARLEVVEGGG
HLFLLTHADESIALIRDFLDAPENAESKAA
>Mature_270_residues
MEEVGGRTLRVAKWRLDAPSDHPPILFFNGIGANIEAVAPLAEVLDDRAFIMFDMPGVGESPDPVLPYNPFTMSWTAAQL
LGRYDIDEVDVLGISWGGAMAQHFALQHGARTRRLVLIATTPGMAMVPGNPAAFTKMANPRRYIDPDFMNEHFATLYGGA
TENPGQKAEHVGRLKPPSPRGYLYQLLCMLGWTSLPALPFLGKETLVMMGDDDQIVPVINGKILARMIPNARLEVVEGGG
HLFLLTHADESIALIRDFLDAPENAESKAA

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 29474; Mature: 29474

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVGGRTLRVAKWRLDAPSDHPPILFFNGIGANIEAVAPLAEVLDDRAFIMFDMPGVGE
CCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCC
SPDPVLPYNPFTMSWTAAQLLGRYDIDEVDVLGISWGGAMAQHFALQHGARTRRLVLIAT
CCCCCCCCCCCEECHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCEEEEEEEE
TPGMAMVPGNPAAFTKMANPRRYIDPDFMNEHFATLYGGATENPGQKAEHVGRLKPPSPR
CCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCC
GYLYQLLCMLGWTSLPALPFLGKETLVMMGDDDQIVPVINGKILARMIPNARLEVVEGGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEECCEEEEECCCCCEEEEEECCC
HLFLLTHADESIALIRDFLDAPENAESKAA
EEEEEEECCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEEVGGRTLRVAKWRLDAPSDHPPILFFNGIGANIEAVAPLAEVLDDRAFIMFDMPGVGE
CCCCCCCEEEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCC
SPDPVLPYNPFTMSWTAAQLLGRYDIDEVDVLGISWGGAMAQHFALQHGARTRRLVLIAT
CCCCCCCCCCCEECHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCEEEEEEEE
TPGMAMVPGNPAAFTKMANPRRYIDPDFMNEHFATLYGGATENPGQKAEHVGRLKPPSPR
CCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCC
GYLYQLLCMLGWTSLPALPFLGKETLVMMGDDDQIVPVINGKILARMIPNARLEVVEGGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEECCEEEEECCCCCEEEEEECCC
HLFLLTHADESIALIRDFLDAPENAESKAA
EEEEEEECCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]