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The map label for this gene is ddlB [C]

Identifier: 85374184

GI number: 85374184

Start: 1405509

End: 1406519

Strand: Direct

Name: ddlB [C]

Synonym: ELI_06785

Alternate gene names: 85374184

Gene position: 1405509-1406519 (Clockwise)

Preceding gene: 85374182

Following gene: 85374186

Centisome position: 46.05

GC content: 61.72

Gene sequence:

>1011_bases
ATGATCGGTATCCAACCCCTCCGCATCCCGGCGGAGGCCAAGGAGCGGGTGCGCCTGCTCTTCATGGCCAAACATGCCTT
GTGGGGTGGCGGTATGCACCCCGAAGACGGCAACCACGCCATCTACCACCACGAGGTGCGCAGCACGCTGGAAGGCATGG
GCCTCAATCTCGCGCTGGCCGACACTTACCGGGTGCTGTTCGATACGCCCGACGCCGACTTCGTATTCCCTTTGCTCAAT
CGCGGCGGGTTCGTGAACAGCGAGATGCTGATCCCGACGCTTTGCAACATGCACCGCATCCCCTATCTCGGCGCCATGCC
GTTCCTGCGCGGCCTCGGCGACGACAAGTCCGTCTCCAAGCTCGTGAGTACCCATGCCGGCGTGCCTACGGCGGACTGGT
TCTGCTTCCGCCGCGGCGCGCCCGTCGAGGAAGCGAGCCTGCCCGCTGCCCCCGACGGACGCTGGGTCATCAAGCCCAAC
GCCTCCTCGGCCAGCTGGGGCATCTCCGACGCTTTCGATTTCCAGGGCGTCGCTAACGCCATCGCCAACATTCACGGACA
GGGGCACGATGCCATCGTCGAACCCTATCTCGACGGCTACGACGTGCAGTGCGCCTTCATCACCATCGATGGCGAAGCGC
GGGCCCTGCCCATGCTGTGGTACGAGCGCGAAGACACGCAGAAACTGTGGACCTATTACGAGAAACGCGACCTCGTCCAG
AACACCGAGAAAGCCGCGCTCAAGACCTTCGATAACCCGGACTTCGCACCGCTCATTACCGATATGGCGCGGAAGGTCGC
GCGCGAATTACTGCCTTTCGACTATGGCCGCATCGAATTCCGTGTCGATCTCGAAAAAGGCGACATCAATTTTATTGAGA
TCAATCTCAACTGCAATCTGTGGTCGGAGAAGGTGATGGCGAAGGCAGCGAAGTTCGCCGGTTTCAGCCACGCGGACCTG
CTGGAAACGCTGCTCGCGGAAAGCTGGCGTCGCAACGGGCTGATTGCCTGA

Upstream 100 bases:

>100_bases
CGCCGCTCACACCGCTCTGCATGGCGCTTTTCGCTTCCTGCCTCTTGATCGAAGCTGTATGCGCGCGCGCCAAAGGTCGC
CCATAAGAGGACGTCGCAAC

Downstream 100 bases:

>100_bases
GACTTACAAACCCAAGCCCGTTCGGGCTGAGGTCCTGAGCTGGTCGAAGGGCGAAGCCCAGCCGCAACATTTAGCCTGCC
CTTCGACAGGTTCAGGGCGA

Product: hypothetical protein

Products: ADP; D-Alanyl-D-alanine; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MIGIQPLRIPAEAKERVRLLFMAKHALWGGGMHPEDGNHAIYHHEVRSTLEGMGLNLALADTYRVLFDTPDADFVFPLLN
RGGFVNSEMLIPTLCNMHRIPYLGAMPFLRGLGDDKSVSKLVSTHAGVPTADWFCFRRGAPVEEASLPAAPDGRWVIKPN
ASSASWGISDAFDFQGVANAIANIHGQGHDAIVEPYLDGYDVQCAFITIDGEARALPMLWYEREDTQKLWTYYEKRDLVQ
NTEKAALKTFDNPDFAPLITDMARKVARELLPFDYGRIEFRVDLEKGDINFIEINLNCNLWSEKVMAKAAKFAGFSHADL
LETLLAESWRRNGLIA

Sequences:

>Translated_336_residues
MIGIQPLRIPAEAKERVRLLFMAKHALWGGGMHPEDGNHAIYHHEVRSTLEGMGLNLALADTYRVLFDTPDADFVFPLLN
RGGFVNSEMLIPTLCNMHRIPYLGAMPFLRGLGDDKSVSKLVSTHAGVPTADWFCFRRGAPVEEASLPAAPDGRWVIKPN
ASSASWGISDAFDFQGVANAIANIHGQGHDAIVEPYLDGYDVQCAFITIDGEARALPMLWYEREDTQKLWTYYEKRDLVQ
NTEKAALKTFDNPDFAPLITDMARKVARELLPFDYGRIEFRVDLEKGDINFIEINLNCNLWSEKVMAKAAKFAGFSHADL
LETLLAESWRRNGLIA
>Mature_336_residues
MIGIQPLRIPAEAKERVRLLFMAKHALWGGGMHPEDGNHAIYHHEVRSTLEGMGLNLALADTYRVLFDTPDADFVFPLLN
RGGFVNSEMLIPTLCNMHRIPYLGAMPFLRGLGDDKSVSKLVSTHAGVPTADWFCFRRGAPVEEASLPAAPDGRWVIKPN
ASSASWGISDAFDFQGVANAIANIHGQGHDAIVEPYLDGYDVQCAFITIDGEARALPMLWYEREDTQKLWTYYEKRDLVQ
NTEKAALKTFDNPDFAPLITDMARKVARELLPFDYGRIEFRVDLEKGDINFIEINLNCNLWSEKVMAKAAKFAGFSHADL
LETLLAESWRRNGLIA

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4 [C]

Molecular weight: Translated: 37640; Mature: 37640

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGIQPLRIPAEAKERVRLLFMAKHALWGGGMHPEDGNHAIYHHEVRSTLEGMGLNLALA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCEEEEE
DTYRVLFDTPDADFVFPLLNRGGFVNSEMLIPTLCNMHRIPYLGAMPFLRGLGDDKSVSK
CEEEEEEECCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHH
LVSTHAGVPTADWFCFRRGAPVEEASLPAAPDGRWVIKPNASSASWGISDAFDFQGVANA
HHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
IANIHGQGHDAIVEPYLDGYDVQCAFITIDGEARALPMLWYEREDTQKLWTYYEKRDLVQ
HHHCCCCCCCCEECCCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
NTEKAALKTFDNPDFAPLITDMARKVARELLPFDYGRIEFRVDLEKGDINFIEINLNCNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEEEECCH
WSEKVMAKAAKFAGFSHADLLETLLAESWRRNGLIA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIGIQPLRIPAEAKERVRLLFMAKHALWGGGMHPEDGNHAIYHHEVRSTLEGMGLNLALA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCEEEEE
DTYRVLFDTPDADFVFPLLNRGGFVNSEMLIPTLCNMHRIPYLGAMPFLRGLGDDKSVSK
CEEEEEEECCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHH
LVSTHAGVPTADWFCFRRGAPVEEASLPAAPDGRWVIKPNASSASWGISDAFDFQGVANA
HHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
IANIHGQGHDAIVEPYLDGYDVQCAFITIDGEARALPMLWYEREDTQKLWTYYEKRDLVQ
HHHCCCCCCCCEECCCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
NTEKAALKTFDNPDFAPLITDMARKVARELLPFDYGRIEFRVDLEKGDINFIEINLNCNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEEEECCH
WSEKVMAKAAKFAGFSHADLLETLLAESWRRNGLIA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1018 [C]

Specific activity: 31

Km value (mM): 15 {D-norleucine}} 6 {D-Phe}} 86 {D-Ala}} 70.3 {D-Ala}} 60.4 {D-Ala}} 57.5 {D-Ala}} 43.7 {D-Ala}} 38 {D-Ala}} 25.7 {D-Ala}} 22.9 {D-Ala}} 22.8 {D-Ala}} 20.9 {D-Ala}} 6.4 {D-Ala}} 5.4 {D-Ala}} 1.2 {D

Substrates: D-Alanine; ATP [C]

Specific reaction: (2) D-Alanine + ATP <==> ADP + D-Alanyl-D-alanine + H+ + Phosphate [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: [1 (S)-Aminoethyl] [(2RS) 2-carboxy-1-octyl] phosphinic acid; Aminoalkyl phosphinate; D-Ala-D-2-hydroxy; D-Ala-D-Ala; D-Ala-D-Alaphosphinate; D-Cycloserine; Gly; Gly-Gly; Phosphinates; Phosphonate dipeptide analogs [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]