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The map label for this gene is rne [H]

Identifier: 85374182

GI number: 85374182

Start: 1401934

End: 1404666

Strand: Direct

Name: rne [H]

Synonym: ELI_06775

Alternate gene names: 85374182

Gene position: 1401934-1404666 (Clockwise)

Preceding gene: 85374172

Following gene: 85374184

Centisome position: 45.93

GC content: 62.61

Gene sequence:

>2733_bases
ATGGCAACGCGCATGCTTATCGATGCGCGCCACACGGAAGAAACCCGGGTGGCGGTGCTCAAAGGCAACCGGATTGAGGA
ATTCGATTTCGAGTCTGCTGAACACAAGCAGATCAAAGGCAATATCTACCTGGCCAAAGTCACGAGGGTCGAACCGTCGC
TGCAGGCGGCGTTCGTAGATTTCGGAGGCAACCGTCACGGTTTCCTCGCCTTTAGCGAAATCCATCCCGACTATTACCAG
ATCCCGCAGGCGGACCGCGAGAAGCTGCTCGCGGAAGAGGCCGAAGCCGCCGAGGAAGAGGCGCGTCTGCGCGCCGAGGA
AGAAGATCGCGGCGAAATGCCGGGCGACGAATACGACGCCGAGGACGAAAGCTCCGGAGCGCTCGCCGAAGATCTGGCCG
AAGACGGCCTGGAGGAAATCGACACGTCCGAGAAGGACAAGGTTGCGACGATCGAGGAAGGCCAGCTCGACAGCGACGAT
GATGACGACGACGATTCGGACGATGACGATTCCGACGACGACGATGACGACGACAATAACGGCGACAACGGCAAGTCCAG
GGGACGCCGCGGACGTGGTCGTGGCCGCGGTCGGCACCAGGGCAAAGGGAAGGGTCGCGGTGGCAAGTCCCGGGCCAAGG
AAGTCGATGCAGTTCGCGCCAAGCGCCAGGCGCTGCGTCGCCGGTACAAGATCCAGGACGTTATCCAGCGCCGCCAGGTG
ATGTTGGTGCAGGTCGTCAAGGAAGAGCGCGGCAACAAGGGCGCGGCGCTGACGACCTATCTCAGCCTTGCCGGTCGCTA
CACCGTCCTTATGCCCAATTCGTCGCACGGCGGCGGGATCAGCCGCAAGATTTCGAGCGCCAGCGATCGCAAGCGGTTGA
AGCAGGTCGTCTCCGACCTGAGCCTGCCCAAGCAGATGGGCCTGATCGTCCGCACCGCCGGCCTCAGCCGCACCAAGACG
GAAATCAAGCGCGACTTCGACTACCTCGCGCGTCTTTGGGACGAGATCCGCGAGAAGACGCTGTCTTCGAGCGCGCCGAG
CCTGATCCATTCGGACAGCGACCTCATCAAGCGCGCGATCCGCGATATCTACAATCGCGAGATCGAGGAAGTCGTGGTCG
AGGGCGAGGAAGGCTACAAGCTCGCCAAGAGCTTCATGAAGCTGCTGATGCCGAGCCATGCGCGGCGGGTGAAAGCCTAT
TCCGACCCGGTGCCGCTGTTCCAGCGCTACGGTGCGGAGGACCAGCTGCGCGCCATGTACGATCCGGTCGTGCAGCTCAA
ATCGGGCGGCTACCTGGTCATCAACCCGACCGAGGCACTTGTTTCGATCGACATCAACTCGGGCCGTTCCACCAAGGAGC
ACGGGATCGAGCAGACCGCGACAGCGACCAATATCGAAGCGGCCAAGGAAATCGCCCGTCAGCTGCGCCTGCGCGACATG
GCCGGGCTCATCGTGATCGACTTCATCGACATGGAATACAATTCCAATGTCCGGAAGGTCGAGCGCGCGATGAAGGACGC
GCTCAAGAACGATCGGGCGCGCATCCAAGTCGGCCGCATCTCCGGCTTCGGCCTGATGGAAATGAGCCGCCAGCGCCTGC
GTACCGGGGTTCTGGAAGCGACCACCCGCGATTGCCCGCATTGCGACGGCACCGGTCTCGTCCGCACCGCGTCCAGCGCG
GGTCTCTCGGCGTTGCGCCTGATCGAGGACGAAGCAGCCAAGGGCAAGGGCACGGTCATCTCGCTCAAGGCGAGTACGGA
AGCGGCAGTGTATTTGCTCAATTCCAAGCGCGCCGACCTTCAGGAGATCGAAGACCGCTACAACGTGACGGTCGAAGTGG
TCCCCGAAGGCGAGGACGAAGGCGCCAAGATGAGCGTCGGCAGTTCCGGTCCGCGCCCGTCGGAAGCTCCCAAGTTCGAG
CCGATCGTCGATGATGACGACGACGAGGAAGAGATCGAAGACGACGCGTCGGACGATGATGACGAGGACGATGACGGCCA
GCCAAGGAAGCGTCGCCGCCGCCGTCGCGGCGGGCGTGGTCGCAACAAGAACAAGCAGAACGACCGTTCCGACGGAGATG
ACGACGATTCCGACGAGGACGATTCAGACGACAGTGAAACGTCCGACGACGACGATGACGGAAAGCCCAAGAAGCGTCGC
CGCCGTGGCGGTCGCCGTCGTGGCGGACGTGGACGCAACAAGGGCGATGCCGACAGCGATTCGAAAGACGATGAAAGCCC
GCTCGAAGAGGTGTCCGAACAAGTGAAGGAAGACATGCCCGTCGTTGCCGGTCCAGACGGTTCGCCGGAAACCGCCGAGG
CCGCTGCCGAAGAAGAAAAGCCGAAGAAGCGTCGCGCGCCTCGCAAGAAGAAGGCGGACGAAGACACCAAGGCCCAGGCA
TCCGCCGACGACAAGTCCGAAGAAAAGCCCAAGCGCCGCCCGCGCAAGAAGAAGGTCGACGAGGCTGCGGAAAAGGCAGA
CGACAAGGCTTCGGAAAAGGCTGCCAAAAAGGAAACCGAGGCCAAGCCCAAGCCGAAACGGGCCACCCGCAGCAAGAAGC
CTGCGGCCGGCAACGACGAAGGCGAGGCATCGAAGACCGGGAAAGGCGACGCTGCTGCGAAACCTGAAGCGGAAACGGAC
AAGGGAGCCGGCGGCAAGGCTGCGAACGAAAAAACCGATGGCGATGGGAAACCCCGCCGCGGCTGGTGGCAGCGCACCTT
CGGTGACGAATAA

Upstream 100 bases:

>100_bases
GGCAGTAGCCGCGGCGGTTTCCACACATATCTGCGCGAGCGCTCGACACCAGTGGGTCTCAGGACCCTGGCGATACCGCT
CGGCGCACGGAGAAAATTAC

Downstream 100 bases:

>100_bases
TTGCTAGAAACAGGTGGGCGCGGCGGTCAGGCCCAGGCCTCGATCACGCGCCCACTTTTCGCCCGATACCCCATTCGTTC
CAGCCGATCGACTTGGGCGT

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 910; Mature: 909

Protein sequence:

>910_residues
MATRMLIDARHTEETRVAVLKGNRIEEFDFESAEHKQIKGNIYLAKVTRVEPSLQAAFVDFGGNRHGFLAFSEIHPDYYQ
IPQADREKLLAEEAEAAEEEARLRAEEEDRGEMPGDEYDAEDESSGALAEDLAEDGLEEIDTSEKDKVATIEEGQLDSDD
DDDDDSDDDDSDDDDDDDNNGDNGKSRGRRGRGRGRGRHQGKGKGRGGKSRAKEVDAVRAKRQALRRRYKIQDVIQRRQV
MLVQVVKEERGNKGAALTTYLSLAGRYTVLMPNSSHGGGISRKISSASDRKRLKQVVSDLSLPKQMGLIVRTAGLSRTKT
EIKRDFDYLARLWDEIREKTLSSSAPSLIHSDSDLIKRAIRDIYNREIEEVVVEGEEGYKLAKSFMKLLMPSHARRVKAY
SDPVPLFQRYGAEDQLRAMYDPVVQLKSGGYLVINPTEALVSIDINSGRSTKEHGIEQTATATNIEAAKEIARQLRLRDM
AGLIVIDFIDMEYNSNVRKVERAMKDALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEATTRDCPHCDGTGLVRTASSA
GLSALRLIEDEAAKGKGTVISLKASTEAAVYLLNSKRADLQEIEDRYNVTVEVVPEGEDEGAKMSVGSSGPRPSEAPKFE
PIVDDDDDEEEIEDDASDDDDEDDDGQPRKRRRRRRGGRGRNKNKQNDRSDGDDDDSDEDDSDDSETSDDDDDGKPKKRR
RRGGRRRGGRGRNKGDADSDSKDDESPLEEVSEQVKEDMPVVAGPDGSPETAEAAAEEEKPKKRRAPRKKKADEDTKAQA
SADDKSEEKPKRRPRKKKVDEAAEKADDKASEKAAKKETEAKPKPKRATRSKKPAAGNDEGEASKTGKGDAAAKPEAETD
KGAGGKAANEKTDGDGKPRRGWWQRTFGDE

Sequences:

>Translated_910_residues
MATRMLIDARHTEETRVAVLKGNRIEEFDFESAEHKQIKGNIYLAKVTRVEPSLQAAFVDFGGNRHGFLAFSEIHPDYYQ
IPQADREKLLAEEAEAAEEEARLRAEEEDRGEMPGDEYDAEDESSGALAEDLAEDGLEEIDTSEKDKVATIEEGQLDSDD
DDDDDSDDDDSDDDDDDDNNGDNGKSRGRRGRGRGRGRHQGKGKGRGGKSRAKEVDAVRAKRQALRRRYKIQDVIQRRQV
MLVQVVKEERGNKGAALTTYLSLAGRYTVLMPNSSHGGGISRKISSASDRKRLKQVVSDLSLPKQMGLIVRTAGLSRTKT
EIKRDFDYLARLWDEIREKTLSSSAPSLIHSDSDLIKRAIRDIYNREIEEVVVEGEEGYKLAKSFMKLLMPSHARRVKAY
SDPVPLFQRYGAEDQLRAMYDPVVQLKSGGYLVINPTEALVSIDINSGRSTKEHGIEQTATATNIEAAKEIARQLRLRDM
AGLIVIDFIDMEYNSNVRKVERAMKDALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEATTRDCPHCDGTGLVRTASSA
GLSALRLIEDEAAKGKGTVISLKASTEAAVYLLNSKRADLQEIEDRYNVTVEVVPEGEDEGAKMSVGSSGPRPSEAPKFE
PIVDDDDDEEEIEDDASDDDDEDDDGQPRKRRRRRRGGRGRNKNKQNDRSDGDDDDSDEDDSDDSETSDDDDDGKPKKRR
RRGGRRRGGRGRNKGDADSDSKDDESPLEEVSEQVKEDMPVVAGPDGSPETAEAAAEEEKPKKRRAPRKKKADEDTKAQA
SADDKSEEKPKRRPRKKKVDEAAEKADDKASEKAAKKETEAKPKPKRATRSKKPAAGNDEGEASKTGKGDAAAKPEAETD
KGAGGKAANEKTDGDGKPRRGWWQRTFGDE
>Mature_909_residues
ATRMLIDARHTEETRVAVLKGNRIEEFDFESAEHKQIKGNIYLAKVTRVEPSLQAAFVDFGGNRHGFLAFSEIHPDYYQI
PQADREKLLAEEAEAAEEEARLRAEEEDRGEMPGDEYDAEDESSGALAEDLAEDGLEEIDTSEKDKVATIEEGQLDSDDD
DDDDSDDDDSDDDDDDDNNGDNGKSRGRRGRGRGRGRHQGKGKGRGGKSRAKEVDAVRAKRQALRRRYKIQDVIQRRQVM
LVQVVKEERGNKGAALTTYLSLAGRYTVLMPNSSHGGGISRKISSASDRKRLKQVVSDLSLPKQMGLIVRTAGLSRTKTE
IKRDFDYLARLWDEIREKTLSSSAPSLIHSDSDLIKRAIRDIYNREIEEVVVEGEEGYKLAKSFMKLLMPSHARRVKAYS
DPVPLFQRYGAEDQLRAMYDPVVQLKSGGYLVINPTEALVSIDINSGRSTKEHGIEQTATATNIEAAKEIARQLRLRDMA
GLIVIDFIDMEYNSNVRKVERAMKDALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEATTRDCPHCDGTGLVRTASSAG
LSALRLIEDEAAKGKGTVISLKASTEAAVYLLNSKRADLQEIEDRYNVTVEVVPEGEDEGAKMSVGSSGPRPSEAPKFEP
IVDDDDDEEEIEDDASDDDDEDDDGQPRKRRRRRRGGRGRNKNKQNDRSDGDDDDSDEDDSDDSETSDDDDDGKPKKRRR
RGGRRRGGRGRNKGDADSDSKDDESPLEEVSEQVKEDMPVVAGPDGSPETAEAAAEEEKPKKRRAPRKKKADEDTKAQAS
ADDKSEEKPKRRPRKKKVDEAAEKADDKASEKAAKKETEAKPKPKRATRSKKPAAGNDEGEASKTGKGDAAAKPEAETDK
GAGGKAANEKTDGDGKPRRGWWQRTFGDE

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=401, Percent_Identity=46.1346633416459, Blast_Score=345, Evalue=1e-95,
Organism=Escherichia coli, GI87082235, Length=336, Percent_Identity=35.7142857142857, Blast_Score=198, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 100984; Mature: 100853

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRMLIDARHTEETRVAVLKGNRIEEFDFESAEHKQIKGNIYLAKVTRVEPSLQAAFVD
CCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEECCCEEEEEEEECCCCHHEEHEE
FGGNRHGFLAFSEIHPDYYQIPQADREKLLAEEAEAAEEEARLRAEEEDRGEMPGDEYDA
CCCCCCCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
EDESSGALAEDLAEDGLEEIDTSEKDKVATIEEGQLDSDDDDDDDSDDDDSDDDDDDDNN
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDNGKSRGRRGRGRGRGRHQGKGKGRGGKSRAKEVDAVRAKRQALRRRYKIQDVIQRRQV
CCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVQVVKEERGNKGAALTTYLSLAGRYTVLMPNSSHGGGISRKISSASDRKRLKQVVSDL
HHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLPKQMGLIVRTAGLSRTKTEIKRDFDYLARLWDEIREKTLSSSAPSLIHSDSDLIKRAI
CCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHH
RDIYNREIEEVVVEGEEGYKLAKSFMKLLMPSHARRVKAYSDPVPLFQRYGAEDQLRAMY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHH
DPVVQLKSGGYLVINPTEALVSIDINSGRSTKEHGIEQTATATNIEAAKEIARQLRLRDM
HHHHEECCCCEEEECCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AGLIVIDFIDMEYNSNVRKVERAMKDALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEA
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
TTRDCPHCDGTGLVRTASSAGLSALRLIEDEAAKGKGTVISLKASTEAAVYLLNSKRADL
HCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCCHH
QEIEDRYNVTVEVVPEGEDEGAKMSVGSSGPRPSEAPKFEPIVDDDDDEEEIEDDASDDD
HHHHHHHCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
DEDDDGQPRKRRRRRRGGRGRNKNKQNDRSDGDDDDSDEDDSDDSETSDDDDDGKPKKRR
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RRGGRRRGGRGRNKGDADSDSKDDESPLEEVSEQVKEDMPVVAGPDGSPETAEAAAEEEK
HCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHC
PKKRRAPRKKKADEDTKAQASADDKSEEKPKRRPRKKKVDEAAEKADDKASEKAAKKETE
HHHHCCCCHHCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AKPKPKRATRSKKPAAGNDEGEASKTGKGDAAAKPEAETDKGAGGKAANEKTDGDGKPRR
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GWWQRTFGDE
CHHHHCCCCC
>Mature Secondary Structure 
ATRMLIDARHTEETRVAVLKGNRIEEFDFESAEHKQIKGNIYLAKVTRVEPSLQAAFVD
CCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEECCCEEEEEEEECCCCHHEEHEE
FGGNRHGFLAFSEIHPDYYQIPQADREKLLAEEAEAAEEEARLRAEEEDRGEMPGDEYDA
CCCCCCCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
EDESSGALAEDLAEDGLEEIDTSEKDKVATIEEGQLDSDDDDDDDSDDDDSDDDDDDDNN
CCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDNGKSRGRRGRGRGRGRHQGKGKGRGGKSRAKEVDAVRAKRQALRRRYKIQDVIQRRQV
CCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVQVVKEERGNKGAALTTYLSLAGRYTVLMPNSSHGGGISRKISSASDRKRLKQVVSDL
HHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLPKQMGLIVRTAGLSRTKTEIKRDFDYLARLWDEIREKTLSSSAPSLIHSDSDLIKRAI
CCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHH
RDIYNREIEEVVVEGEEGYKLAKSFMKLLMPSHARRVKAYSDPVPLFQRYGAEDQLRAMY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHH
DPVVQLKSGGYLVINPTEALVSIDINSGRSTKEHGIEQTATATNIEAAKEIARQLRLRDM
HHHHEECCCCEEEECCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AGLIVIDFIDMEYNSNVRKVERAMKDALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEA
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
TTRDCPHCDGTGLVRTASSAGLSALRLIEDEAAKGKGTVISLKASTEAAVYLLNSKRADL
HCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCCCHH
QEIEDRYNVTVEVVPEGEDEGAKMSVGSSGPRPSEAPKFEPIVDDDDDEEEIEDDASDDD
HHHHHHHCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
DEDDDGQPRKRRRRRRGGRGRNKNKQNDRSDGDDDDSDEDDSDDSETSDDDDDGKPKKRR
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RRGGRRRGGRGRNKGDADSDSKDDESPLEEVSEQVKEDMPVVAGPDGSPETAEAAAEEEK
HCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHC
PKKRRAPRKKKADEDTKAQASADDKSEEKPKRRPRKKKVDEAAEKADDKASEKAAKKETE
HHHHCCCCHHCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AKPKPKRATRSKKPAAGNDEGEASKTGKGDAAAKPEAETDKGAGGKAANEKTDGDGKPRR
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GWWQRTFGDE
CHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]