Click here to switch to the map view.

The map label for this gene is amtB [H]

Identifier: 85374172

GI number: 85374172

Start: 1391558

End: 1392931

Strand: Direct

Name: amtB [H]

Synonym: ELI_06725

Alternate gene names: 85374172

Gene position: 1391558-1392931 (Clockwise)

Preceding gene: 85374171

Following gene: 85374182

Centisome position: 45.59

GC content: 65.43

Gene sequence:

>1374_bases
GTGCGCGCGGTTAAACACGCCATAACCTTCGGCCGATATGCGCGCTGCCTGACCATCCTGCGCGCCGAGGGGGATACGAT
GCGTCGCTTGTTTTCCGCAGCAGTACTCGCAAGCTTGCCCGGCACGCTCGCCGCGCAGGACGTGACGCAGATCGCGCTCA
GCGATGGTGGCGATACCGCCTGGATCCTGACCGCCTCCGCGCTGGTGCTGATGATGACCCTGCCGGGCCTCGGGCTGTTT
TACGGCGGGCTGGTGAGAGCGAAGAATTTCCTCTCGGTTCTGCTGCAGGTCGGCGCGGTCGCTTCGGTCGCTTCGGTCTT
GTGGGTCGTCGTCGGCTATACCCTCGCTTTCGGCGCGGTAACCAATGGGTGGCTCGGCGCGGGCAATGCATGGATGCTGA
TCGATCTCGCCAGTGTTCGTGAGGGCCTGACCATACCCGAAAGCACGTTCGCCCTGTTCCAGATGACCTTTGCCGTGATC
ACGCCGGCCCTGATGGTCGGCGCCTGGGTCGAGCGTGCACGTTTCGGCTGGGTCGTCGGGTTCTGTGCGCTGTGGGGCTT
GGTCGTCTACGCTCCGGTCGCGCACTGGGTCTGGGGTGGAGGCTGGCTCTCAGGCTTCGGTGTGCTCGATTTTGCCGGCG
GTATCGTCGTGCATACGACCGCAGGGGTCTCCGCGCTGGTGATCGCCCTGCTGCTGGGCCGTCGCGACGGTTTCCCGAAA
TCGCCGCTGCTCCCGCATGCGCCGGCTCTGACCATGGCAGGTGCCGCGCTGCTGTGGGTCGGCTGGTTCGGCTTCAACGG
TGGCAGCGCGCTGACCGCCACCGACGACGCTTCGACCGCGATCATCAACACCCATGTGGCAGCAGCTACGGCAACGCTGG
TCTGGTTGCTGATCGAAAAGGTGAGTCTGGGCAAGCCTACCAGCGTCGGCTGGGCAACCGGAGCCATTGCGGGCCTCGCC
ACGATCACGCCTGCGGCGGGCTTCGTCGGCCCGGGCGCGGCGATCGTGATGGGCGTGCTCGCTTCGGTGGTCTGCTATTA
CGCGATCCAGCTATTCAAGCAGAAGCTGCACATCGACGATTCGCTCGATGTCTTTGCCGTCCACGGCGTCGGCGGCATGC
TCGGCTCGCTTCTCCTGGCGGTATTCCTGTCAACGAGCTTCGGCGGCCTCGGCTACGGCGAAGGCGCGACGATGGTGTCG
CAGCTCATCGCCCAGGCGATCGGCATCGGCGCGGTGGCCCTGTTCAGCGCAGTCGCCACCGCGATCCTCGCGCTGATGGT
CAGTGTGATCTTCCCCATGCGCGCCAGCGAAGACGACGAGCGGAACGGCCTCGATATCGCAGCTCATGGCGAGCGAGCCT
GGGATCTCGATTAG

Upstream 100 bases:

>100_bases
CGACGGCCACCGCCCGCCCGACCAGGTCCTCGACGCGCTGGTCTGACATCCCCGCACCGCGCAACCGTCAAACTGCCGGA
TAACCGGCAGCATTCCGCTT

Downstream 100 bases:

>100_bases
AGCTCTAGGTGGAAAAAACGGTTGAAATCGCTCCCGACATAGTCGGCAAAAGGTTGGCCGGGCTTGAAACCGAACGAGCG
ATATAGCCCCTCCGCCGGTG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRAVKHAITFGRYARCLTILRAEGDTMRRLFSAAVLASLPGTLAAQDVTQIALSDGGDTAWILTASALVLMMTLPGLGLF
YGGLVRAKNFLSVLLQVGAVASVASVLWVVVGYTLAFGAVTNGWLGAGNAWMLIDLASVREGLTIPESTFALFQMTFAVI
TPALMVGAWVERARFGWVVGFCALWGLVVYAPVAHWVWGGGWLSGFGVLDFAGGIVVHTTAGVSALVIALLLGRRDGFPK
SPLLPHAPALTMAGAALLWVGWFGFNGGSALTATDDASTAIINTHVAAATATLVWLLIEKVSLGKPTSVGWATGAIAGLA
TITPAAGFVGPGAAIVMGVLASVVCYYAIQLFKQKLHIDDSLDVFAVHGVGGMLGSLLLAVFLSTSFGGLGYGEGATMVS
QLIAQAIGIGAVALFSAVATAILALMVSVIFPMRASEDDERNGLDIAAHGERAWDLD

Sequences:

>Translated_457_residues
MRAVKHAITFGRYARCLTILRAEGDTMRRLFSAAVLASLPGTLAAQDVTQIALSDGGDTAWILTASALVLMMTLPGLGLF
YGGLVRAKNFLSVLLQVGAVASVASVLWVVVGYTLAFGAVTNGWLGAGNAWMLIDLASVREGLTIPESTFALFQMTFAVI
TPALMVGAWVERARFGWVVGFCALWGLVVYAPVAHWVWGGGWLSGFGVLDFAGGIVVHTTAGVSALVIALLLGRRDGFPK
SPLLPHAPALTMAGAALLWVGWFGFNGGSALTATDDASTAIINTHVAAATATLVWLLIEKVSLGKPTSVGWATGAIAGLA
TITPAAGFVGPGAAIVMGVLASVVCYYAIQLFKQKLHIDDSLDVFAVHGVGGMLGSLLLAVFLSTSFGGLGYGEGATMVS
QLIAQAIGIGAVALFSAVATAILALMVSVIFPMRASEDDERNGLDIAAHGERAWDLD
>Mature_457_residues
MRAVKHAITFGRYARCLTILRAEGDTMRRLFSAAVLASLPGTLAAQDVTQIALSDGGDTAWILTASALVLMMTLPGLGLF
YGGLVRAKNFLSVLLQVGAVASVASVLWVVVGYTLAFGAVTNGWLGAGNAWMLIDLASVREGLTIPESTFALFQMTFAVI
TPALMVGAWVERARFGWVVGFCALWGLVVYAPVAHWVWGGGWLSGFGVLDFAGGIVVHTTAGVSALVIALLLGRRDGFPK
SPLLPHAPALTMAGAALLWVGWFGFNGGSALTATDDASTAIINTHVAAATATLVWLLIEKVSLGKPTSVGWATGAIAGLA
TITPAAGFVGPGAAIVMGVLASVVCYYAIQLFKQKLHIDDSLDVFAVHGVGGMLGSLLLAVFLSTSFGGLGYGEGATMVS
QLIAQAIGIGAVALFSAVATAILALMVSVIFPMRASEDDERNGLDIAAHGERAWDLD

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=422, Percent_Identity=43.3649289099526, Blast_Score=324, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=28.9351851851852, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=435, Percent_Identity=27.816091954023, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=424, Percent_Identity=26.6509433962264, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=24.7030878859857, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=428, Percent_Identity=38.785046728972, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325396, Length=413, Percent_Identity=32.4455205811138, Blast_Score=199, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6321559, Length=413, Percent_Identity=32.2033898305085, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=27.536231884058, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47308; Mature: 47308

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVKHAITFGRYARCLTILRAEGDTMRRLFSAAVLASLPGTLAAQDVTQIALSDGGDTA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
WILTASALVLMMTLPGLGLFYGGLVRAKNFLSVLLQVGAVASVASVLWVVVGYTLAFGAV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNGWLGAGNAWMLIDLASVREGLTIPESTFALFQMTFAVITPALMVGAWVERARFGWVVG
CCCCCCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCALWGLVVYAPVAHWVWGGGWLSGFGVLDFAGGIVVHTTAGVSALVIALLLGRRDGFPK
HHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCC
SPLLPHAPALTMAGAALLWVGWFGFNGGSALTATDDASTAIINTHVAAATATLVWLLIEK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VSLGKPTSVGWATGAIAGLATITPAAGFVGPGAAIVMGVLASVVCYYAIQLFKQKLHIDD
HCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLDVFAVHGVGGMLGSLLLAVFLSTSFGGLGYGEGATMVSQLIAQAIGIGAVALFSAVAT
CCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AILALMVSVIFPMRASEDDERNGLDIAAHGERAWDLD
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MRAVKHAITFGRYARCLTILRAEGDTMRRLFSAAVLASLPGTLAAQDVTQIALSDGGDTA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
WILTASALVLMMTLPGLGLFYGGLVRAKNFLSVLLQVGAVASVASVLWVVVGYTLAFGAV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNGWLGAGNAWMLIDLASVREGLTIPESTFALFQMTFAVITPALMVGAWVERARFGWVVG
CCCCCCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCALWGLVVYAPVAHWVWGGGWLSGFGVLDFAGGIVVHTTAGVSALVIALLLGRRDGFPK
HHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCC
SPLLPHAPALTMAGAALLWVGWFGFNGGSALTATDDASTAIINTHVAAATATLVWLLIEK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VSLGKPTSVGWATGAIAGLATITPAAGFVGPGAAIVMGVLASVVCYYAIQLFKQKLHIDD
HCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLDVFAVHGVGGMLGSLLLAVFLSTSFGGLGYGEGATMVSQLIAQAIGIGAVALFSAVAT
CCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AILALMVSVIFPMRASEDDERNGLDIAAHGERAWDLD
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]