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The map label for this gene is gltX1 [H]

Identifier: 85374135

GI number: 85374135

Start: 1357014

End: 1358393

Strand: Direct

Name: gltX1 [H]

Synonym: ELI_06540

Alternate gene names: 85374135

Gene position: 1357014-1358393 (Clockwise)

Preceding gene: 85374133

Following gene: 85374136

Centisome position: 44.46

GC content: 60.51

Gene sequence:

>1380_bases
TTGCACCTGGGCGGCGCGCGCACCGCGTTGTTCAATTGGCTATTCGCGCGCCACTACGGCGGCAAGGCGCTTCTACGCAT
CGAAGATACCGACAAGAAGCGCTCGACCCAAGATGCCATCGACAAAATTCTCGAGGGTCTCGATTGGCTCGGCCTCGACT
ATGACGATACGCCGCTGTTCCAATCGGAACGCGGATCCCGCCATGTCGAAGTGGCGGAGAAGCTGCTTGAATATGGCTAT
GCCTATCGCTGCTACGCCACGCAGGAAGAGCTTGAGCAAATGCGGGCCGCTCAACGCGCGGCCAAACAACCGCAACGCTA
TGACGGCCGCTGGCGTCATCGAGATGCCTCGGAAGCCCCTGCAGGCTCCCCTTTCGTCGTCCGATTGAAAACGGCTGAAG
ATGGTGAGACGACTATCGAAGATAAAGTGCAGGGCAGGGTCACTGTCCGCAATGAAGAATTGGATGACTATATCCTGCTG
CGCGCTGATGGCTCGCCGACCTACATGCTCGCCGTAGTCGTCGACGACCATGACATGGGCGTGACCCATGTTATCCGTGG
CGACGATCATCTCAACAACGCCTTCCGGCAGCTGCCGATCTACCATGCGATGGACACCATCGAAGGCGGCTGGCCCGAGC
CGGTCTATGCACATGTCCCGCTGATCCACGGCCAAGACGGTGCCAAGCTGTCCAAGCGGCATGGCGCGGTCGGCGTCGAG
GCCTATCGCGACGAAATGGGCGTGCTTCCCGAGGCACTGTTCAATTACCTCCTCCGCCTCGGCTGGGGGCACGGCGACCA
GGAAGAATTCACACGAGAACAAGCCATCGAGCTCTTTACGCTGGAAGGAGTTGGAAAGAGTGCCTCGCGCTTCGATACGA
AAAAGCTGCTCAACCTCAACGGACACCATATCCGCGAGGCGGACAATGCGCGCCTCGCAGCGCTGGTTGCGGCCAAGATT
GGCCCGGCGGCGGACGAAGCTTTGCTGACGCAAGCGATGGAGGTTCTCAAGGTCCGAGCCAAGGACATCAACGAACTGGC
CGAGGGCGCAGCGTTCCTGTTCAGCAAACGCCCGCTTGAAATGACCGAAAAGGCGGAGAAGCTGCTCGACGGGGAAGGCC
GCGAAAGATTGGCGAAGGTTTCCGAGGCTTTGAGCGCTGAAAATGACTGGACAATCGAGGCCCTCGAAGCCACTACGAAA
GCGCTCGCCGAGCGCCTCGAACTGGGCCTTGGCAAGCTGGCGCAGCCCATGCGTGCAGCTTTAACGGGGACGACGACGTC
CCCCGGAATTTTCGATGTGCTCGTCCTGCTCGGCAGAGAGGAGTCCCTGGCGCGGATAGACGCGCAGGCGACCCCGGCCG
AAGAAGGCGCGAACGGATAG

Upstream 100 bases:

>100_bases
ATGATCTCGATTGGAAAGGAAGCCACGCAGGATGGCAAGTAAAGATGACGCTGGAACGAGTAATGTCGTCACCCGTTTCG
CCCCATCACCTACCGGATAT

Downstream 100 bases:

>100_bases
ACTGATTTTAGGAGAACACGACAATGGCAGACACGGCAAAGCTAGAGCTGAATGGCCAGTCCCATGAGTTTCCCGTGTTG
CAAGGCACGACCGGCCCCGA

Product: glutamyl- and glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MHLGGARTALFNWLFARHYGGKALLRIEDTDKKRSTQDAIDKILEGLDWLGLDYDDTPLFQSERGSRHVEVAEKLLEYGY
AYRCYATQEELEQMRAAQRAAKQPQRYDGRWRHRDASEAPAGSPFVVRLKTAEDGETTIEDKVQGRVTVRNEELDDYILL
RADGSPTYMLAVVVDDHDMGVTHVIRGDDHLNNAFRQLPIYHAMDTIEGGWPEPVYAHVPLIHGQDGAKLSKRHGAVGVE
AYRDEMGVLPEALFNYLLRLGWGHGDQEEFTREQAIELFTLEGVGKSASRFDTKKLLNLNGHHIREADNARLAALVAAKI
GPAADEALLTQAMEVLKVRAKDINELAEGAAFLFSKRPLEMTEKAEKLLDGEGRERLAKVSEALSAENDWTIEALEATTK
ALAERLELGLGKLAQPMRAALTGTTTSPGIFDVLVLLGREESLARIDAQATPAEEGANG

Sequences:

>Translated_459_residues
MHLGGARTALFNWLFARHYGGKALLRIEDTDKKRSTQDAIDKILEGLDWLGLDYDDTPLFQSERGSRHVEVAEKLLEYGY
AYRCYATQEELEQMRAAQRAAKQPQRYDGRWRHRDASEAPAGSPFVVRLKTAEDGETTIEDKVQGRVTVRNEELDDYILL
RADGSPTYMLAVVVDDHDMGVTHVIRGDDHLNNAFRQLPIYHAMDTIEGGWPEPVYAHVPLIHGQDGAKLSKRHGAVGVE
AYRDEMGVLPEALFNYLLRLGWGHGDQEEFTREQAIELFTLEGVGKSASRFDTKKLLNLNGHHIREADNARLAALVAAKI
GPAADEALLTQAMEVLKVRAKDINELAEGAAFLFSKRPLEMTEKAEKLLDGEGRERLAKVSEALSAENDWTIEALEATTK
ALAERLELGLGKLAQPMRAALTGTTTSPGIFDVLVLLGREESLARIDAQATPAEEGANG
>Mature_459_residues
MHLGGARTALFNWLFARHYGGKALLRIEDTDKKRSTQDAIDKILEGLDWLGLDYDDTPLFQSERGSRHVEVAEKLLEYGY
AYRCYATQEELEQMRAAQRAAKQPQRYDGRWRHRDASEAPAGSPFVVRLKTAEDGETTIEDKVQGRVTVRNEELDDYILL
RADGSPTYMLAVVVDDHDMGVTHVIRGDDHLNNAFRQLPIYHAMDTIEGGWPEPVYAHVPLIHGQDGAKLSKRHGAVGVE
AYRDEMGVLPEALFNYLLRLGWGHGDQEEFTREQAIELFTLEGVGKSASRFDTKKLLNLNGHHIREADNARLAALVAAKI
GPAADEALLTQAMEVLKVRAKDINELAEGAAFLFSKRPLEMTEKAEKLLDGEGRERLAKVSEALSAENDWTIEALEATTK
ALAERLELGLGKLAQPMRAALTGTTTSPGIFDVLVLLGREESLARIDAQATPAEEGANG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=29.4117647058824, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1788743, Length=456, Percent_Identity=44.7368421052632, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI226510925, Length=240, Percent_Identity=34.5833333333333, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=470, Percent_Identity=29.7872340425532, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=476, Percent_Identity=32.563025210084, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24665321, Length=484, Percent_Identity=32.4380165289256, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 50921; Mature: 50921

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLGGARTALFNWLFARHYGGKALLRIEDTDKKRSTQDAIDKILEGLDWLGLDYDDTPLF
CCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCHHCCCCCCCCCCCC
QSERGSRHVEVAEKLLEYGYAYRCYATQEELEQMRAAQRAAKQPQRYDGRWRHRDASEAP
CCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AGSPFVVRLKTAEDGETTIEDKVQGRVTVRNEELDDYILLRADGSPTYMLAVVVDDHDMG
CCCCEEEEEEECCCCCCCHHHHHCCEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCC
VTHVIRGDDHLNNAFRQLPIYHAMDTIEGGWPEPVYAHVPLIHGQDGAKLSKRHGAVGVE
EEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCHH
AYRDEMGVLPEALFNYLLRLGWGHGDQEEFTREQAIELFTLEGVGKSASRFDTKKLLNLN
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCC
GHHIREADNARLAALVAAKIGPAADEALLTQAMEVLKVRAKDINELAEGAAFLFSKRPLE
CCEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
MTEKAEKLLDGEGRERLAKVSEALSAENDWTIEALEATTKALAERLELGLGKLAQPMRAA
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGTTTSPGIFDVLVLLGREESLARIDAQATPAEEGANG
HCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHLGGARTALFNWLFARHYGGKALLRIEDTDKKRSTQDAIDKILEGLDWLGLDYDDTPLF
CCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCHHCCCCCCCCCCCC
QSERGSRHVEVAEKLLEYGYAYRCYATQEELEQMRAAQRAAKQPQRYDGRWRHRDASEAP
CCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AGSPFVVRLKTAEDGETTIEDKVQGRVTVRNEELDDYILLRADGSPTYMLAVVVDDHDMG
CCCCEEEEEEECCCCCCCHHHHHCCEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCC
VTHVIRGDDHLNNAFRQLPIYHAMDTIEGGWPEPVYAHVPLIHGQDGAKLSKRHGAVGVE
EEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCHH
AYRDEMGVLPEALFNYLLRLGWGHGDQEEFTREQAIELFTLEGVGKSASRFDTKKLLNLN
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCC
GHHIREADNARLAALVAAKIGPAADEALLTQAMEVLKVRAKDINELAEGAAFLFSKRPLE
CCEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
MTEKAEKLLDGEGRERLAKVSEALSAENDWTIEALEATTKALAERLELGLGKLAQPMRAA
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGTTTSPGIFDVLVLLGREESLARIDAQATPAEEGANG
HCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA