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The map label for this gene is lexA

Identifier: 85374133

GI number: 85374133

Start: 1353944

End: 1354645

Strand: Direct

Name: lexA

Synonym: ELI_06530

Alternate gene names: 85374133

Gene position: 1353944-1354645 (Clockwise)

Preceding gene: 85374132

Following gene: 85374135

Centisome position: 44.36

GC content: 61.25

Gene sequence:

>702_bases
ATGCTGACGGCAAAGCAGCACGAATTGATCCGCTTTATCCAGCAACGGCTGGAGGAGACGGGAATTTCGCCATCTTTCGA
GGAAATGAAGGAGGCGCTCGATCTCAAGAGCAAGTCTGGGGTGCATCGCCTGATTTCCGCGCTCGAAGAACGCGGTTTCA
TCCGTCGCCTGCCCAACCGGGCGCGCGCGCTGGAAATTCTCAAACAGCCGGAAGATGTCGTAGGTGGCGGCGCCAAGGCC
GCACAGTCAGGTTCGGAAGCCAGCAATGTGGTCGATATTCGTACCGCGCAGGCCAAGACCGTACCGGCTCCCATCAATGA
CGTGGTCGAAATTCCGCTCCACGGTCGTATCGCCGCCGGCGCGCCGATCGAGGCATTGGAAGATCACCAGTCGCTGCCCG
TTCCCGCCGCCCTGCTCGGTCCGGGCGATCACTACGCGCTTGAAGTCTCTGGCGATTCCATGATCGAGGCGGGCATCTTC
GACGGCGACTTCGCCCTTATCCGCCGCACGGACTCCGCACGCGATGGCGAGATTGTCGTGGCGCTGGTCAATAACGAGGA
AGCGACGCTCAAATACCTGCATCGCGACAGCGGCAGGGTCCGGCTCGATCCGGCCAATGCCAGTTATGAAGCGCAGGTCT
ACGATCCGCATCAGGTGCAGGTGCAGGGCAAGCTCGCCGGTTTGCTGCGCCGATACCACTGA

Upstream 100 bases:

>100_bases
GACTTACGAAAATTGGTTCCTTAATTGTTCCACATTCGTTCGCATTCGCGCGCGAAGTTGGAACGGGCCATTTGCGAGCC
CAAAGAGGGAGCCAACACCC

Downstream 100 bases:

>100_bases
TCTGCACAAGCTAGGGGGCGCGGCACTGTCCCCGCAACTGCGATCCCGCTCGCAGCAGGCTTCCCCGCCTACCGCCGCGC
CCCCTTCTCCCGACCTCTCC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLTAKQHELIRFIQQRLEETGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNRARALEILKQPEDVVGGGAKA
AQSGSEASNVVDIRTAQAKTVPAPINDVVEIPLHGRIAAGAPIEALEDHQSLPVPAALLGPGDHYALEVSGDSMIEAGIF
DGDFALIRRTDSARDGEIVVALVNNEEATLKYLHRDSGRVRLDPANASYEAQVYDPHQVQVQGKLAGLLRRYH

Sequences:

>Translated_233_residues
MLTAKQHELIRFIQQRLEETGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNRARALEILKQPEDVVGGGAKA
AQSGSEASNVVDIRTAQAKTVPAPINDVVEIPLHGRIAAGAPIEALEDHQSLPVPAALLGPGDHYALEVSGDSMIEAGIF
DGDFALIRRTDSARDGEIVVALVNNEEATLKYLHRDSGRVRLDPANASYEAQVYDPHQVQVQGKLAGLLRRYH
>Mature_233_residues
MLTAKQHELIRFIQQRLEETGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNRARALEILKQPEDVVGGGAKA
AQSGSEASNVVDIRTAQAKTVPAPINDVVEIPLHGRIAAGAPIEALEDHQSLPVPAALLGPGDHYALEVSGDSMIEAGIF
DGDFALIRRTDSARDGEIVVALVNNEEATLKYLHRDSGRVRLDPANASYEAQVYDPHQVQVQGKLAGLLRRYH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=234, Percent_Identity=27.3504273504274, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_ERYLH (Q2NA93)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458195.1
- ProteinModelPortal:   Q2NA93
- SMR:   Q2NA93
- STRING:   Q2NA93
- MEROPS:   S24.001
- GeneID:   3870312
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06530
- NMPDR:   fig|314225.3.peg.1748
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- PhylomeDB:   Q2NA93
- ProtClustDB:   PRK00215
- BioCyc:   ELIT314225:ELI_06530-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25420; Mature: 25420

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: ACT_SITE 153-153 ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAKQHELIRFIQQRLEETGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCH
ARALEILKQPEDVVGGGAKAAQSGSEASNVVDIRTAQAKTVPAPINDVVEIPLHGRIAAG
HHHHHHHHCCHHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEEECC
APIEALEDHQSLPVPAALLGPGDHYALEVSGDSMIEAGIFDGDFALIRRTDSARDGEIVV
CCHHHHHCCCCCCCCEEEECCCCCEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCEEE
ALVNNEEATLKYLHRDSGRVRLDPANASYEAQVYDPHQVQVQGKLAGLLRRYH
EEECCCCHHHHHHCCCCCEEEECCCCCCEEEEEECCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MLTAKQHELIRFIQQRLEETGISPSFEEMKEALDLKSKSGVHRLISALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCH
ARALEILKQPEDVVGGGAKAAQSGSEASNVVDIRTAQAKTVPAPINDVVEIPLHGRIAAG
HHHHHHHHCCHHHHCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEEECC
APIEALEDHQSLPVPAALLGPGDHYALEVSGDSMIEAGIFDGDFALIRRTDSARDGEIVV
CCHHHHHCCCCCCCCEEEECCCCCEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCEEE
ALVNNEEATLKYLHRDSGRVRLDPANASYEAQVYDPHQVQVQGKLAGLLRRYH
EEECCCCHHHHHHCCCCCEEEECCCCCCEEEEEECCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA