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The map label for this gene is ccsA [H]

Identifier: 85374136

GI number: 85374136

Start: 1358417

End: 1359700

Strand: Direct

Name: ccsA [H]

Synonym: ELI_06545

Alternate gene names: 85374136

Gene position: 1358417-1359700 (Clockwise)

Preceding gene: 85374135

Following gene: 85374137

Centisome position: 44.5

GC content: 60.75

Gene sequence:

>1284_bases
ATGGCAGACACGGCAAAGCTAGAGCTGAATGGCCAGTCCCATGAGTTTCCCGTGTTGCAAGGCACGACCGGCCCCGATGT
GGTCGACATCCGCAAGCTTTACGGTGCCACCGGTGCCTTCACCTTCGATCCCGGCTACAAATCCACCGCCAGTTGTGAAA
GCGCGCTGACCTATATCGACGGCAACGAGGGCGTGCTGCTGCATCGCGGCTACCCGATCGGCCAGCTGGCCGAACAATCG
AGCTTCATGGAAGTAAGCTACCTGCTGCTCAACGGCGAGCTGCCGAAGCAGGACGAGCTCGATGACTTCACTTACACCAT
CACCCGTCACACCATGCTGCACGACCAGCTGCGACAGTTCTACCAGGGCTTCCGCCGCGATGCGCACCCGATGGCGATCA
TGTGCGGCGTCGTGGGCGCGCTGTCGGCGTTCTATCACGACAGCACCGACATTTCCGATCCCGAGCACCGCAAGATCAGC
AGCCACCGTCTGATCGCCAAGATGCCGACGATTGCCGCCATGGCCTACAAATACGCCGTCGGTCAGCCCTTCCTACAGCC
GAAGAACGACTTGAGCTATACGGGCAATTTCCTGCGCATGACCTTCGGTGTTCCAGCAGAGGATTACGAAATCCACCCCG
CTATCGAGCGAGCGATGGATCGCATCTTCATTCTGCACGCCGATCACGAACAGAACGCGTCGACCTCGACCGTGCGCCTT
GCCGGCTCGTCGGGGGCCAACCCCTTCGCGTGTATTGCTGCCGGAATTGCGTGCCTGTGGGGCCCGGCGCACGGCGGTGC
CAACGAAGCCGCGCTCAACATGTTGCAGGAAATCGGCAGCCCCGATCGCATCCCGCACTATATCGAGCGCGCGAAGGACA
AGAATGACCCCTTCCGCCTGATGGGCTTCGGCCACCGGGTCTACAAGAATTACGATCCGCGTGCGACCGTGATGCAGCAG
ACGCTGCGCGAGGTCTTCGAAGCGCTCAACGTCCAGGATCCGGTCTTCGAAGTCGCGCTTCAGCTGGAAGAGATCGCCCT
CAACGACGATTACTTCAGGGAAAAGAAGCTCTTCCCGAATGTCGACTTCTACTCGGGTATCATCCTGAACGCGATCGGCT
TCCCGACCACCATGTTCACTGCGCTGTTCGCCCTTGCCCGTACGGTCGGCTGGGTCGCACAGTGGAATGAGATGATCTCC
GATCCAGGCCAGGTCATCGGTCGCCCGCGCCAGCTCTACACCGGGCCGACCGAGCGCGACTACGTGCCGATCGGTCAACG
CTAA

Upstream 100 bases:

>100_bases
TCCTGCTCGGCAGAGAGGAGTCCCTGGCGCGGATAGACGCGCAGGCGACCCCGGCCGAAGAAGGCGCGAACGGATAGACT
GATTTTAGGAGAACACGACA

Downstream 100 bases:

>100_bases
GCGACAGGGCGCATGCGCCTTCCGGGGTTCCTGTTGCAGATCGCGGGCCTGACGATGCTCCTCACAGGGGCATGGTGGGT
ATTGCAGGGCCTTTCGATCA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MADTAKLELNGQSHEFPVLQGTTGPDVVDIRKLYGATGAFTFDPGYKSTASCESALTYIDGNEGVLLHRGYPIGQLAEQS
SFMEVSYLLLNGELPKQDELDDFTYTITRHTMLHDQLRQFYQGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPEHRKIS
SHRLIAKMPTIAAMAYKYAVGQPFLQPKNDLSYTGNFLRMTFGVPAEDYEIHPAIERAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLQEIGSPDRIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQQ
TLREVFEALNVQDPVFEVALQLEEIALNDDYFREKKLFPNVDFYSGIILNAIGFPTTMFTALFALARTVGWVAQWNEMIS
DPGQVIGRPRQLYTGPTERDYVPIGQR

Sequences:

>Translated_427_residues
MADTAKLELNGQSHEFPVLQGTTGPDVVDIRKLYGATGAFTFDPGYKSTASCESALTYIDGNEGVLLHRGYPIGQLAEQS
SFMEVSYLLLNGELPKQDELDDFTYTITRHTMLHDQLRQFYQGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPEHRKIS
SHRLIAKMPTIAAMAYKYAVGQPFLQPKNDLSYTGNFLRMTFGVPAEDYEIHPAIERAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLQEIGSPDRIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQQ
TLREVFEALNVQDPVFEVALQLEEIALNDDYFREKKLFPNVDFYSGIILNAIGFPTTMFTALFALARTVGWVAQWNEMIS
DPGQVIGRPRQLYTGPTERDYVPIGQR
>Mature_426_residues
ADTAKLELNGQSHEFPVLQGTTGPDVVDIRKLYGATGAFTFDPGYKSTASCESALTYIDGNEGVLLHRGYPIGQLAEQSS
FMEVSYLLLNGELPKQDELDDFTYTITRHTMLHDQLRQFYQGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPEHRKISS
HRLIAKMPTIAAMAYKYAVGQPFLQPKNDLSYTGNFLRMTFGVPAEDYEIHPAIERAMDRIFILHADHEQNASTSTVRLA
GSSGANPFACIAAGIACLWGPAHGGANEAALNMLQEIGSPDRIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQQT
LREVFEALNVQDPVFEVALQLEEIALNDDYFREKKLFPNVDFYSGIILNAIGFPTTMFTALFALARTVGWVAQWNEMISD
PGQVIGRPRQLYTGPTERDYVPIGQR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=351, Percent_Identity=28.2051282051282, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786939, Length=423, Percent_Identity=67.3758865248227, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1786527, Length=381, Percent_Identity=31.496062992126, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17555174, Length=416, Percent_Identity=25.7211538461538, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319850, Length=355, Percent_Identity=28.7323943661972, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324328, Length=350, Percent_Identity=27.7142857142857, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=363, Percent_Identity=26.9972451790634, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640124, Length=428, Percent_Identity=26.8691588785047, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24640126, Length=428, Percent_Identity=26.8691588785047, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356863, Length=346, Percent_Identity=26.878612716763, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47720; Mature: 47588

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTAKLELNGQSHEFPVLQGTTGPDVVDIRKLYGATGAFTFDPGYKSTASCESALTYID
CCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHEEEEE
GNEGVLLHRGYPIGQLAEQSSFMEVSYLLLNGELPKQDELDDFTYTITRHTMLHDQLRQF
CCCCEEEECCCCHHHHHHHCCHHEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
YQGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPEHRKISSHRLIAKMPTIAAMAYKYAV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHC
GQPFLQPKNDLSYTGNFLRMTFGVPAEDYEIHPAIERAMDRIFILHADHEQNASTSTVRL
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEECHHHHHHHHEEEEEECCCCCCCCCCEEEE
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLQEIGSPDRIPHYIERAKDKNDPFRL
ECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEE
MGFGHRVYKNYDPRATVMQQTLREVFEALNVQDPVFEVALQLEEIALNDDYFREKKLFPN
EECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCC
VDFYSGIILNAIGFPTTMFTALFALARTVGWVAQWNEMISDPGQVIGRPRQLYTGPTERD
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCC
YVPIGQR
CCCCCCC
>Mature Secondary Structure 
ADTAKLELNGQSHEFPVLQGTTGPDVVDIRKLYGATGAFTFDPGYKSTASCESALTYID
CCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHEEEEE
GNEGVLLHRGYPIGQLAEQSSFMEVSYLLLNGELPKQDELDDFTYTITRHTMLHDQLRQF
CCCCEEEECCCCHHHHHHHCCHHEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
YQGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPEHRKISSHRLIAKMPTIAAMAYKYAV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHC
GQPFLQPKNDLSYTGNFLRMTFGVPAEDYEIHPAIERAMDRIFILHADHEQNASTSTVRL
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEECHHHHHHHHEEEEEECCCCCCCCCCEEEE
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLQEIGSPDRIPHYIERAKDKNDPFRL
ECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEE
MGFGHRVYKNYDPRATVMQQTLREVFEALNVQDPVFEVALQLEEIALNDDYFREKKLFPN
EECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCC
VDFYSGIILNAIGFPTTMFTALFALARTVGWVAQWNEMISDPGQVIGRPRQLYTGPTERD
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCC
YVPIGQR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]