Click here to switch to the map view.

The map label for this gene is ppdK [H]

Identifier: 85374001

GI number: 85374001

Start: 1213238

End: 1215931

Strand: Direct

Name: ppdK [H]

Synonym: ELI_05870

Alternate gene names: 85374001

Gene position: 1213238-1215931 (Clockwise)

Preceding gene: 85374000

Following gene: 85374003

Centisome position: 39.75

GC content: 63.36

Gene sequence:

>2694_bases
ATGTCCCAGACTGTCTATCCTTTCGGTGGAAATGCAAAGTCGGACGACCCGCGCGCCAAGGACAAGACGATCGTCGGGGG
CAAGGGCGCGAACCTCGCCGAGATGGCCGAGATCGGCCTGCCGGTCCCACCGGGCTTCACGATCACCACCGAAGAATGCC
TCGACTATCTCGAGCATGGCGCGGACCCTTCGACTGAGCTCAGGGCAGGCTTCGCCGAAAAGCTGCGTCATGACGTCACT
TCCGCGCTCGGCCATATCGAAGCAACGGTCGGTAAGCGCCTGGGTGACAAGGACGATCCGCTGCTGGTTTCGGTAAGGTC
GGGTGCGGCGATCTCTATGCCCGGCATGATGGACACGGTCCTCAATCTCGGCCTCAACGACGAGACGGTCGAGGGGTTGG
CGCAGGTGAGCGGCGACGAGCGCTTCGCCTGGGACAGCTATCGCCGCTTCATCCAGATGTACTCCGATGTCGTCCTCGGG
ATCGACCATGGCCTGTTCGAGGAAGCACTCGAAATCGCCAAGGAAGACAAGGACCTGTACGCCGATACGGAGATGTCGGC
AGAAGAGTGGAAGGAACTCGTCGCCGAGTACAAGCAGATCGCGGCGGACGAGCGGGGCGGGGAGCCGTTCCCGCAAGATG
TGACCGAACAGCTCTGGGGCGCGATCCGCGCGGTATTCGACAGCTGGGACAGCGAAAGGGCGAAAATATACCGCCGTCTC
AACGACATACCGCACGACATGGGCACGGCGGTGAACGTTCAGGCCATGGTCTTCGGCAATATGGGCGACACCTCGGCCAC
CGGTGTCGCCTTCACACGTGACCCGGCGACAGGCGAGAAGTCCTATTACGGCGAATTTCTGATCAACGCGCAGGGCGAAG
ACGTGGTGGCGGGCATCCGCACTCCGCAGTATCTCACCAAGGCCGCGCGCGAAGCCGCCGGGGCCGATAAGCCGAGCATG
GAAGAGGCGATGCCCGAAGCCTATGCCGAACTCGCCCGCGTATTCGACCTTCTTGAGCAACACTATCGCGACATGCAGGA
TATCGAGTTCACCGTGGAGCGCGGTCACCTGTGGATGCTGCAAACGCGGACCGGCAAGCGGACCGCCAAGGCTGCTCTCA
AGCTGGCGGTCGACATGGCGGATGAAGGGCTGATCGACGAAAAGACCGCGATCCTGCGGGTCGATCCCATGGCGCTCGAC
CAGCTGCTCCACCCGACACTCGATCCCGACGCTCCGCGCGATGTGCTGACCACCGGTCTGCCGGCTTCTCCGGGCGCGGC
GGCGGGCAAGATCGTGCTCGATGCAGACACCGCCGAACAATGGGCCGGGCGGGGTGAGAGCGTGATCCTGGTGCGGGTCG
AGACCAGCCCCGAAGACATCCATGGCATGCACGCTGCGACGGGAATCCTGACTGCGCGTGGCGGCATGACGTCGCACGCC
GCCGTCGTGGCGCGCGGGATGGGGCGGCCCTGTGTGTCGGGAGCTTCTGCCGTCGTGATCGACCGCGCCAAGCGTTTGCT
CAAAATCGGATCGCGCGATTTGAAGGAAGGGGACCTCATCACGCTCGACGGCGCGAAGGGTCAGGTGATGGCGGGCGAGG
TCGCGACGATCGAGCCTGAACTCGCGGGCGATTTCGGCACGCTGATGGAATGGGCCGACAAACATCGCCGGATGCGCGTG
CGGACCAATGCCGAAACCCCCAATGACTGTAGGATGGCGCGGCAATTCGGGGCCGAGGGTATCGGCCTGTGCCGCACCGA
ACACATGTTCTTCGATGCCGGTCGCATCAGCGCCGTGCGTGAAATGATCCTCGCCGATGACGAGAACGGTCGCCGTGCCG
CGCTCGACAAGCTGCTGCCCGAGCAACGCAAGGACTTCCACGAGATTTTCGAGGTCATGAAGGGGCTATCCTGCACGATC
CGGCTGCTCGATCCGCCGCTGCACGAATTCTTGCCACATGGTGATGCCGAATTCGAAGATTTGGCAGCAACTACAGGGCA
TTCGGTCGAGCACTTGAAGCGTCGGGCGGGGGAGCTTCACGAGTTTAATCCCATGCTAGGGCACCGTGGGTGTCGCCTCG
GCATCACCTATCCCGAGATCTACGAGATGCAGGCGCGGGCGATTTTCGAAGCGGCCTGCGACGTGAAGGCGGCTTCCGGC
GAAGCGCCGTTGCCTGAAGTGATGATCCCGCTGGTGGTGACCAGGCGCGAGCTGGAGATTCTTCGCGCGCTGGTGGAGCG
GGTTGCTGGCGACGTCTTCGGCGAAAAGGGCACCGTGGTCGATTACCTTGTCGGCACCATGATCGAGCTGCCCCGGGCCG
CGCTGATGGCGGGTGAGATTGCCGAGGAAGCCCGCTTCTTCTCCTTCGGAACCAATGACTTGACCCAGACGACTCTCGGC
GTTTCGAGGGACGATGCGAGCAGGTTCCTCGGGCCTTACGTCGATCGCGGCATCTTCGCCCGCGATCCTTTCGTCAGTCT
CGATATCGATGGCGTCGGCCAGCTGGTGACTATGGCGGCGGAGCGCGGCCGCGCGACCCGCGGCGACATCAAGCTCGGCA
TCTGCGGCGAACATGGCGGCGATCCGGCGAGCATCCGTTTCTGCGAAGAAACCGGGCTCGATTACGTTTCCGCTTCGCCG
TATCGCGTTCCCATCGCACGGCTCGCAGCGGCGCAGGCATCGCTCGGAAAATAG

Upstream 100 bases:

>100_bases
GTTCTGGAAAAATCCAGCCGGGACAGCGAGTTACAATCGATAGGTGAGGGTGACAGGGTGTAACCTGTGTCACCCGGTTT
TCACCAAAGTAGGAAATTTT

Downstream 100 bases:

>100_bases
CCTATTTCCAGCCTGTTAGAACAAGTATTTCGAAAGTCTCGGCGACTTTCCCGTCGCCGTCCCGCATTCCGGCAAATGCT
CTTAACGCGCGCTGCCATGC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 897; Mature: 896

Protein sequence:

>897_residues
MSQTVYPFGGNAKSDDPRAKDKTIVGGKGANLAEMAEIGLPVPPGFTITTEECLDYLEHGADPSTELRAGFAEKLRHDVT
SALGHIEATVGKRLGDKDDPLLVSVRSGAAISMPGMMDTVLNLGLNDETVEGLAQVSGDERFAWDSYRRFIQMYSDVVLG
IDHGLFEEALEIAKEDKDLYADTEMSAEEWKELVAEYKQIAADERGGEPFPQDVTEQLWGAIRAVFDSWDSERAKIYRRL
NDIPHDMGTAVNVQAMVFGNMGDTSATGVAFTRDPATGEKSYYGEFLINAQGEDVVAGIRTPQYLTKAAREAAGADKPSM
EEAMPEAYAELARVFDLLEQHYRDMQDIEFTVERGHLWMLQTRTGKRTAKAALKLAVDMADEGLIDEKTAILRVDPMALD
QLLHPTLDPDAPRDVLTTGLPASPGAAAGKIVLDADTAEQWAGRGESVILVRVETSPEDIHGMHAATGILTARGGMTSHA
AVVARGMGRPCVSGASAVVIDRAKRLLKIGSRDLKEGDLITLDGAKGQVMAGEVATIEPELAGDFGTLMEWADKHRRMRV
RTNAETPNDCRMARQFGAEGIGLCRTEHMFFDAGRISAVREMILADDENGRRAALDKLLPEQRKDFHEIFEVMKGLSCTI
RLLDPPLHEFLPHGDAEFEDLAATTGHSVEHLKRRAGELHEFNPMLGHRGCRLGITYPEIYEMQARAIFEAACDVKAASG
EAPLPEVMIPLVVTRRELEILRALVERVAGDVFGEKGTVVDYLVGTMIELPRAALMAGEIAEEARFFSFGTNDLTQTTLG
VSRDDASRFLGPYVDRGIFARDPFVSLDIDGVGQLVTMAAERGRATRGDIKLGICGEHGGDPASIRFCEETGLDYVSASP
YRVPIARLAAAQASLGK

Sequences:

>Translated_897_residues
MSQTVYPFGGNAKSDDPRAKDKTIVGGKGANLAEMAEIGLPVPPGFTITTEECLDYLEHGADPSTELRAGFAEKLRHDVT
SALGHIEATVGKRLGDKDDPLLVSVRSGAAISMPGMMDTVLNLGLNDETVEGLAQVSGDERFAWDSYRRFIQMYSDVVLG
IDHGLFEEALEIAKEDKDLYADTEMSAEEWKELVAEYKQIAADERGGEPFPQDVTEQLWGAIRAVFDSWDSERAKIYRRL
NDIPHDMGTAVNVQAMVFGNMGDTSATGVAFTRDPATGEKSYYGEFLINAQGEDVVAGIRTPQYLTKAAREAAGADKPSM
EEAMPEAYAELARVFDLLEQHYRDMQDIEFTVERGHLWMLQTRTGKRTAKAALKLAVDMADEGLIDEKTAILRVDPMALD
QLLHPTLDPDAPRDVLTTGLPASPGAAAGKIVLDADTAEQWAGRGESVILVRVETSPEDIHGMHAATGILTARGGMTSHA
AVVARGMGRPCVSGASAVVIDRAKRLLKIGSRDLKEGDLITLDGAKGQVMAGEVATIEPELAGDFGTLMEWADKHRRMRV
RTNAETPNDCRMARQFGAEGIGLCRTEHMFFDAGRISAVREMILADDENGRRAALDKLLPEQRKDFHEIFEVMKGLSCTI
RLLDPPLHEFLPHGDAEFEDLAATTGHSVEHLKRRAGELHEFNPMLGHRGCRLGITYPEIYEMQARAIFEAACDVKAASG
EAPLPEVMIPLVVTRRELEILRALVERVAGDVFGEKGTVVDYLVGTMIELPRAALMAGEIAEEARFFSFGTNDLTQTTLG
VSRDDASRFLGPYVDRGIFARDPFVSLDIDGVGQLVTMAAERGRATRGDIKLGICGEHGGDPASIRFCEETGLDYVSASP
YRVPIARLAAAQASLGK
>Mature_896_residues
SQTVYPFGGNAKSDDPRAKDKTIVGGKGANLAEMAEIGLPVPPGFTITTEECLDYLEHGADPSTELRAGFAEKLRHDVTS
ALGHIEATVGKRLGDKDDPLLVSVRSGAAISMPGMMDTVLNLGLNDETVEGLAQVSGDERFAWDSYRRFIQMYSDVVLGI
DHGLFEEALEIAKEDKDLYADTEMSAEEWKELVAEYKQIAADERGGEPFPQDVTEQLWGAIRAVFDSWDSERAKIYRRLN
DIPHDMGTAVNVQAMVFGNMGDTSATGVAFTRDPATGEKSYYGEFLINAQGEDVVAGIRTPQYLTKAAREAAGADKPSME
EAMPEAYAELARVFDLLEQHYRDMQDIEFTVERGHLWMLQTRTGKRTAKAALKLAVDMADEGLIDEKTAILRVDPMALDQ
LLHPTLDPDAPRDVLTTGLPASPGAAAGKIVLDADTAEQWAGRGESVILVRVETSPEDIHGMHAATGILTARGGMTSHAA
VVARGMGRPCVSGASAVVIDRAKRLLKIGSRDLKEGDLITLDGAKGQVMAGEVATIEPELAGDFGTLMEWADKHRRMRVR
TNAETPNDCRMARQFGAEGIGLCRTEHMFFDAGRISAVREMILADDENGRRAALDKLLPEQRKDFHEIFEVMKGLSCTIR
LLDPPLHEFLPHGDAEFEDLAATTGHSVEHLKRRAGELHEFNPMLGHRGCRLGITYPEIYEMQARAIFEAACDVKAASGE
APLPEVMIPLVVTRRELEILRALVERVAGDVFGEKGTVVDYLVGTMIELPRAALMAGEIAEEARFFSFGTNDLTQTTLGV
SRDDASRFLGPYVDRGIFARDPFVSLDIDGVGQLVTMAAERGRATRGDIKLGICGEHGGDPASIRFCEETGLDYVSASPY
RVPIARLAAAQASLGK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=462, Percent_Identity=25.5411255411255, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI1787994, Length=738, Percent_Identity=25.4742547425474, Blast_Score=91, Evalue=5e-19,
Organism=Escherichia coli, GI1788726, Length=514, Percent_Identity=23.9299610894942, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1789193, Length=430, Percent_Identity=24.8837209302326, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI48994992, Length=439, Percent_Identity=25.9681093394077, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97696; Mature: 97565

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVYPFGGNAKSDDPRAKDKTIVGGKGANLAEMAEIGLPVPPGFTITTEECLDYLEHG
CCCCEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCC
ADPSTELRAGFAEKLRHDVTSALGHIEATVGKRLGDKDDPLLVSVRSGAAISMPGMMDTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHH
LNLGLNDETVEGLAQVSGDERFAWDSYRRFIQMYSDVVLGIDHGLFEEALEIAKEDKDLY
HHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
ADTEMSAEEWKELVAEYKQIAADERGGEPFPQDVTEQLWGAIRAVFDSWDSERAKIYRRL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NDIPHDMGTAVNVQAMVFGNMGDTSATGVAFTRDPATGEKSYYGEFLINAQGEDVVAGIR
HCCCHHHCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEECCC
TPQYLTKAAREAAGADKPSMEEAMPEAYAELARVFDLLEQHYRDMQDIEFTVERGHLWML
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCEEEE
QTRTGKRTAKAALKLAVDMADEGLIDEKTAILRVDPMALDQLLHPTLDPDAPRDVLTTGL
EECCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCCHHHHHCCC
PASPGAAAGKIVLDADTAEQWAGRGESVILVRVETSPEDIHGMHAATGILTARGGMTSHA
CCCCCCCCCEEEEECCCHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHEEECCCCCHHH
AVVARGMGRPCVSGASAVVIDRAKRLLKIGSRDLKEGDLITLDGAKGQVMAGEVATIEPE
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCEEEECHH
LAGDFGTLMEWADKHRRMRVRTNAETPNDCRMARQFGAEGIGLCRTEHMFFDAGRISAVR
HCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCCCCEEEECHHEECCHHHHHHH
EMILADDENGRRAALDKLLPEQRKDFHEIFEVMKGLSCTIRLLDPPLHEFLPHGDAEFED
HHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHH
LAATTGHSVEHLKRRAGELHEFNPMLGHRGCRLGITYPEIYEMQARAIFEAACDVKAASG
HHHHCCCHHHHHHHHHCCHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCC
EAPLPEVMIPLVVTRRELEILRALVERVAGDVFGEKGTVVDYLVGTMIELPRAALMAGEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEEARFFSFGTNDLTQTTLGVSRDDASRFLGPYVDRGIFARDPFVSLDIDGVGQLVTMAA
HHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
ERGRATRGDIKLGICGEHGGDPASIRFCEETGLDYVSASPYRVPIARLAAAQASLGK
HCCCCCCCCEEEEEECCCCCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQTVYPFGGNAKSDDPRAKDKTIVGGKGANLAEMAEIGLPVPPGFTITTEECLDYLEHG
CCCEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCC
ADPSTELRAGFAEKLRHDVTSALGHIEATVGKRLGDKDDPLLVSVRSGAAISMPGMMDTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHH
LNLGLNDETVEGLAQVSGDERFAWDSYRRFIQMYSDVVLGIDHGLFEEALEIAKEDKDLY
HHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
ADTEMSAEEWKELVAEYKQIAADERGGEPFPQDVTEQLWGAIRAVFDSWDSERAKIYRRL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NDIPHDMGTAVNVQAMVFGNMGDTSATGVAFTRDPATGEKSYYGEFLINAQGEDVVAGIR
HCCCHHHCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCEEECCC
TPQYLTKAAREAAGADKPSMEEAMPEAYAELARVFDLLEQHYRDMQDIEFTVERGHLWML
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCEEEE
QTRTGKRTAKAALKLAVDMADEGLIDEKTAILRVDPMALDQLLHPTLDPDAPRDVLTTGL
EECCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCCHHHHHCCC
PASPGAAAGKIVLDADTAEQWAGRGESVILVRVETSPEDIHGMHAATGILTARGGMTSHA
CCCCCCCCCEEEEECCCHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHEEECCCCCHHH
AVVARGMGRPCVSGASAVVIDRAKRLLKIGSRDLKEGDLITLDGAKGQVMAGEVATIEPE
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCEEEECHH
LAGDFGTLMEWADKHRRMRVRTNAETPNDCRMARQFGAEGIGLCRTEHMFFDAGRISAVR
HCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCCCCEEEECHHEECCHHHHHHH
EMILADDENGRRAALDKLLPEQRKDFHEIFEVMKGLSCTIRLLDPPLHEFLPHGDAEFED
HHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCHHH
LAATTGHSVEHLKRRAGELHEFNPMLGHRGCRLGITYPEIYEMQARAIFEAACDVKAASG
HHHHCCCHHHHHHHHHCCHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCC
EAPLPEVMIPLVVTRRELEILRALVERVAGDVFGEKGTVVDYLVGTMIELPRAALMAGEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEEARFFSFGTNDLTQTTLGVSRDDASRFLGPYVDRGIFARDPFVSLDIDGVGQLVTMAA
HHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
ERGRATRGDIKLGICGEHGGDPASIRFCEETGLDYVSASPYRVPIARLAAAQASLGK
HCCCCCCCCEEEEEECCCCCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]