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The map label for this gene is glyS [H]

Identifier: 85374000

GI number: 85374000

Start: 1210881

End: 1213109

Strand: Direct

Name: glyS [H]

Synonym: ELI_05865

Alternate gene names: 85374000

Gene position: 1210881-1213109 (Clockwise)

Preceding gene: 85373999

Following gene: 85374001

Centisome position: 39.67

GC content: 64.33

Gene sequence:

>2229_bases
ATGCCTGATTTCCTCCTCGAACTCCGATCCGAAGAAATTCCCGCTCGCATGCAGGCAGGGGCCCGCGACGAATTGGAAAA
GCTGTTCCGCCGCGAAATGGATGCTGCCGGTGTCGAAGCCGGCGAGCTCACCGTCTGGTCCACCCCGCGCCGACTGGCAT
TGATCGCGCGCGATCTCCCCGAGGCGACCCAAGCGGTGCGCGAGGAAGCCAAGGGACCGCCCGAAGGTGCGCCCGATCAG
GCGATCGACGGCTTCTGCCGCAAGAACGGCGTCACCCGCGACCAGCTCGAAATACGCGATGTGAAGGGCCGCAACACCTA
CTTCGCGGTGATCGAAAAGCCGGGCCGGGCGGTGAAGGACCTGCTCGCTGAAGCGATTCCCGCGATCATCCGCGACTTCA
GCTGGCCCAAGTCGATGCGCTGGGGCACGGCCTCGATGAGCACAGAGAGCCTGCGCTGGGTGCGGCCGTTGTCGGGCATC
GTCGCGCTGCTCGGCGACGACGTTGTCGAATGCGAGGTGCACGGCGTCACCAGCGGGGCGGTGACGCTCGGCCACCGATT
CCACCACTCGGGCGACATCACGATCGGCAATGCCGACGATTACGCAATGAAGCTTCGCGCAGGGCATGTGATCGTCGATC
ACGAGCAACGTCAGGACCTGATCCGCGAAGGCGCAGCAAAGGTTGCCGCCGAAGCCGGCCTAAAGTTGGTCGAGGACGAG
GGGCTAGTGATCGAGAATGCCGGCCTCACCGAATGGCCCGTGCCGCTGCTCGGCCGCTTCGAGGAGGATTTTCTCCAGGT
CCCGCCCGAGACGATTCAGCTCACCGCACGGGTGAACCAGAAATACTTCGTTTGTCACCGATCCCCAGCGGAGGCTGGGG
TCTCAGACGATGGTGGACAGCCGCACGAGGTCCCAGCCTCCGCTGGGACTCAGCAATTGGCCAACGCCTTCATCTGCACC
GCCAATATCGAGGCGGAGGACGGCGGCGCGCGCGTGGTAGACGGCAACCGCAAGGTGCTTGCCGCCCGCCTCGCCGATGC
GCGCTTTTTCTGGGACGTCGACCGCAAGAAAACCCTCGCCGAGCACGCCAAGGGGTTGGAGCGGATCACCTTCCACGAGA
AGTTGGGCACCGTCGCCGATAAGGTCGAGCGTGTCGCGAACCTGGCGCGCTGGTTGGTGGAATCGGATATCGTCGTCCCA
GCGAAAGCTGGGACCTCTCTCGGTCAAAGCGCCAAGACGAACGAGATCCCAGCTTCCGCTGGGATGACGAAGGAGGAATT
GGCCGACCTTACCGAACAGGCCGCACGCCTCGCCAAGGCCGACCTCGTCACCGAAATGGTCGGCGAGTTTCCCGAACTGC
AGGGCCTAATGGGCGGCTACTACGCTCGCGCCGAAGGTCTTCCAGACGAAGTAGCCGACGCCATCCGCGATCACTACAAG
CCGGTCGGCCAAGGCGATGAAGTGCCGACCGCGCCGGTGACGGTGGCGGTGAGCTTGGCGGACAAGCTGGATACGCTGCG
CAGCTTCTTCTCGATCGACGAGAAACCCACGGGCTCGAAGGACCCCTTCGCGCTGCGCCGCGCAGCACTGGGGGTAATCC
GCCTGATGACCGAGAACGGCCTGCGGATGAGCGCGGGGCAGGGCGAACTCCTCGAATTCTTCGCCGATCGCCTCAAGGTC
CAGCAGCGCGAGGCTGGCGTCCGCCACGACCTGATCGACGCGGTTTTCGCGCTCGGCGGCGAGGACGATCTCGTTCGCCT
GCTTGCCCGGGTGCATGCGCTCCAGGCCTTCGTGCAGACCGAGGACGGCACCAACCTCCTCGCTGGGTACAAGCGTGCGG
CCAATATCCTCAAGAAGGAGCAATGGGACGCGGAGCCGGAGCATACCGGCGACGAGGACCCGCTCGAGAATGTCGACGAT
CCCGACATGCGCGAAATCTATGCCGAGGCGGTCGAGAAAAAACTTTCCTACACGCCCGAACCGGCCGAGAAGGCGCTGAT
CGATGCGCTGGACAGCGCTGAACCGAAAGCGGTCGCAACCGTCGAAACGGAAGACTTCGCCGCCGCCATGTCGGCTCTCG
CGTCGCTGCGCGCGCCGATCGATGCCTTCTTTGAAGAGGTGATTGTGAACGCCGAAGAAAAGCATGTCCGCGCCGCCCGG
CTCGACCTGCTAGCGCGTTTCCGCGATGCGGTGCACCGGGTGGCCGATTTCAGCCGGATCGAAGGATAA

Upstream 100 bases:

>100_bases
CCATCCGTCATCCCAGCGAAAGCTGGGATCTCTCTTAATCTCGCGCTTCGTTGCGACCAGATCCCAGCTTTCGCTGGGAT
GACGGGAGAGGAGGCTGGCA

Downstream 100 bases:

>100_bases
AGCTGGTCGCAAGGTGTCACCTGTCCATGTTCTGGAAAAATCCAGCCGGGACAGCGAGTTACAATCGATAGGTGAGGGTG
ACAGGGTGTAACCTGTGTCA

Product: glycyl-tRNA synthetase beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 742; Mature: 741

Protein sequence:

>742_residues
MPDFLLELRSEEIPARMQAGARDELEKLFRREMDAAGVEAGELTVWSTPRRLALIARDLPEATQAVREEAKGPPEGAPDQ
AIDGFCRKNGVTRDQLEIRDVKGRNTYFAVIEKPGRAVKDLLAEAIPAIIRDFSWPKSMRWGTASMSTESLRWVRPLSGI
VALLGDDVVECEVHGVTSGAVTLGHRFHHSGDITIGNADDYAMKLRAGHVIVDHEQRQDLIREGAAKVAAEAGLKLVEDE
GLVIENAGLTEWPVPLLGRFEEDFLQVPPETIQLTARVNQKYFVCHRSPAEAGVSDDGGQPHEVPASAGTQQLANAFICT
ANIEAEDGGARVVDGNRKVLAARLADARFFWDVDRKKTLAEHAKGLERITFHEKLGTVADKVERVANLARWLVESDIVVP
AKAGTSLGQSAKTNEIPASAGMTKEELADLTEQAARLAKADLVTEMVGEFPELQGLMGGYYARAEGLPDEVADAIRDHYK
PVGQGDEVPTAPVTVAVSLADKLDTLRSFFSIDEKPTGSKDPFALRRAALGVIRLMTENGLRMSAGQGELLEFFADRLKV
QQREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVQTEDGTNLLAGYKRAANILKKEQWDAEPEHTGDEDPLENVDD
PDMREIYAEAVEKKLSYTPEPAEKALIDALDSAEPKAVATVETEDFAAAMSALASLRAPIDAFFEEVIVNAEEKHVRAAR
LDLLARFRDAVHRVADFSRIEG

Sequences:

>Translated_742_residues
MPDFLLELRSEEIPARMQAGARDELEKLFRREMDAAGVEAGELTVWSTPRRLALIARDLPEATQAVREEAKGPPEGAPDQ
AIDGFCRKNGVTRDQLEIRDVKGRNTYFAVIEKPGRAVKDLLAEAIPAIIRDFSWPKSMRWGTASMSTESLRWVRPLSGI
VALLGDDVVECEVHGVTSGAVTLGHRFHHSGDITIGNADDYAMKLRAGHVIVDHEQRQDLIREGAAKVAAEAGLKLVEDE
GLVIENAGLTEWPVPLLGRFEEDFLQVPPETIQLTARVNQKYFVCHRSPAEAGVSDDGGQPHEVPASAGTQQLANAFICT
ANIEAEDGGARVVDGNRKVLAARLADARFFWDVDRKKTLAEHAKGLERITFHEKLGTVADKVERVANLARWLVESDIVVP
AKAGTSLGQSAKTNEIPASAGMTKEELADLTEQAARLAKADLVTEMVGEFPELQGLMGGYYARAEGLPDEVADAIRDHYK
PVGQGDEVPTAPVTVAVSLADKLDTLRSFFSIDEKPTGSKDPFALRRAALGVIRLMTENGLRMSAGQGELLEFFADRLKV
QQREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVQTEDGTNLLAGYKRAANILKKEQWDAEPEHTGDEDPLENVDD
PDMREIYAEAVEKKLSYTPEPAEKALIDALDSAEPKAVATVETEDFAAAMSALASLRAPIDAFFEEVIVNAEEKHVRAAR
LDLLARFRDAVHRVADFSRIEG
>Mature_741_residues
PDFLLELRSEEIPARMQAGARDELEKLFRREMDAAGVEAGELTVWSTPRRLALIARDLPEATQAVREEAKGPPEGAPDQA
IDGFCRKNGVTRDQLEIRDVKGRNTYFAVIEKPGRAVKDLLAEAIPAIIRDFSWPKSMRWGTASMSTESLRWVRPLSGIV
ALLGDDVVECEVHGVTSGAVTLGHRFHHSGDITIGNADDYAMKLRAGHVIVDHEQRQDLIREGAAKVAAEAGLKLVEDEG
LVIENAGLTEWPVPLLGRFEEDFLQVPPETIQLTARVNQKYFVCHRSPAEAGVSDDGGQPHEVPASAGTQQLANAFICTA
NIEAEDGGARVVDGNRKVLAARLADARFFWDVDRKKTLAEHAKGLERITFHEKLGTVADKVERVANLARWLVESDIVVPA
KAGTSLGQSAKTNEIPASAGMTKEELADLTEQAARLAKADLVTEMVGEFPELQGLMGGYYARAEGLPDEVADAIRDHYKP
VGQGDEVPTAPVTVAVSLADKLDTLRSFFSIDEKPTGSKDPFALRRAALGVIRLMTENGLRMSAGQGELLEFFADRLKVQ
QREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVQTEDGTNLLAGYKRAANILKKEQWDAEPEHTGDEDPLENVDDP
DMREIYAEAVEKKLSYTPEPAEKALIDALDSAEPKAVATVETEDFAAAMSALASLRAPIDAFFEEVIVNAEEKHVRAARL
DLLARFRDAVHRVADFSRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=766, Percent_Identity=32.7676240208877, Blast_Score=339, Evalue=4e-94,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 81150; Mature: 81019

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFLLELRSEEIPARMQAGARDELEKLFRREMDAAGVEAGELTVWSTPRRLALIARDLP
CCCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCH
EATQAVREEAKGPPEGAPDQAIDGFCRKNGVTRDQLEIRDVKGRNTYFAVIEKPGRAVKD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHEEECCCCCCEEEEEECCCHHHHH
LLAEAIPAIIRDFSWPKSMRWGTASMSTESLRWVRPLSGIVALLGDDVVECEVHGVTSGA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCH
VTLGHRFHHSGDITIGNADDYAMKLRAGHVIVDHEQRQDLIREGAAKVAAEAGLKLVEDE
HHHHHHEECCCCEEECCCCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHHCCCEEEECC
GLVIENAGLTEWPVPLLGRFEEDFLQVPPETIQLTARVNQKYFVCHRSPAEAGVSDDGGQ
CEEEECCCCCCCCCHHHCCCCHHHHHCCCHHEEEEEECCCEEEEEECCHHHCCCCCCCCC
PHEVPASAGTQQLANAFICTANIEAEDGGARVVDGNRKVLAARLADARFFWDVDRKKTLA
CCCCCCCCCHHHHHCEEEEEEECEECCCCCEEECCCCCEEHHHHHCCHHEECCHHHHHHH
EHAKGLERITFHEKLGTVADKVERVANLARWLVESDIVVPAKAGTSLGQSAKTNEIPASA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCC
GMTKEELADLTEQAARLAKADLVTEMVGEFPELQGLMGGYYARAEGLPDEVADAIRDHYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PVGQGDEVPTAPVTVAVSLADKLDTLRSFFSIDEKPTGSKDPFALRRAALGVIRLMTENG
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
LRMSAGQGELLEFFADRLKVQQREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVQT
CEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
EDGTNLLAGYKRAANILKKEQWDAEPEHTGDEDPLENVDDPDMREIYAEAVEKKLSYTPE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PAEKALIDALDSAEPKAVATVETEDFAAAMSALASLRAPIDAFFEEVIVNAEEKHVRAAR
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHH
LDLLARFRDAVHRVADFSRIEG
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDFLLELRSEEIPARMQAGARDELEKLFRREMDAAGVEAGELTVWSTPRRLALIARDLP
CCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCH
EATQAVREEAKGPPEGAPDQAIDGFCRKNGVTRDQLEIRDVKGRNTYFAVIEKPGRAVKD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHEEECCCCCCEEEEEECCCHHHHH
LLAEAIPAIIRDFSWPKSMRWGTASMSTESLRWVRPLSGIVALLGDDVVECEVHGVTSGA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCH
VTLGHRFHHSGDITIGNADDYAMKLRAGHVIVDHEQRQDLIREGAAKVAAEAGLKLVEDE
HHHHHHEECCCCEEECCCCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHHCCCEEEECC
GLVIENAGLTEWPVPLLGRFEEDFLQVPPETIQLTARVNQKYFVCHRSPAEAGVSDDGGQ
CEEEECCCCCCCCCHHHCCCCHHHHHCCCHHEEEEEECCCEEEEEECCHHHCCCCCCCCC
PHEVPASAGTQQLANAFICTANIEAEDGGARVVDGNRKVLAARLADARFFWDVDRKKTLA
CCCCCCCCCHHHHHCEEEEEEECEECCCCCEEECCCCCEEHHHHHCCHHEECCHHHHHHH
EHAKGLERITFHEKLGTVADKVERVANLARWLVESDIVVPAKAGTSLGQSAKTNEIPASA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCC
GMTKEELADLTEQAARLAKADLVTEMVGEFPELQGLMGGYYARAEGLPDEVADAIRDHYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PVGQGDEVPTAPVTVAVSLADKLDTLRSFFSIDEKPTGSKDPFALRRAALGVIRLMTENG
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
LRMSAGQGELLEFFADRLKVQQREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVQT
CEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
EDGTNLLAGYKRAANILKKEQWDAEPEHTGDEDPLENVDDPDMREIYAEAVEKKLSYTPE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PAEKALIDALDSAEPKAVATVETEDFAAAMSALASLRAPIDAFFEEVIVNAEEKHVRAAR
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHH
LDLLARFRDAVHRVADFSRIEG
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA