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The map label for this gene is purA

Identifier: 85373226

GI number: 85373226

Start: 444868

End: 446157

Strand: Direct

Name: purA

Synonym: ELI_01995

Alternate gene names: 85373226

Gene position: 444868-446157 (Clockwise)

Preceding gene: 85373222

Following gene: 85373228

Centisome position: 14.57

GC content: 64.03

Gene sequence:

>1290_bases
ATGGCTAACGTAACCGTAATCGGCGCCCAGTGGGGCGATGAGGGCAAGGGCAAGATCGTCGACTGGCTGGCCGACCGCGC
CGATGCGGTCGTACGGTTTCAGGGTGGGCACAATGCCGGCCACACGCTGGTGGTCGATGGCACCACTTACAAGCTGAGCC
TGCTGCCGAGCGGGATCGTGTCGGGCACGTTGTCGATAATCGGCAATGGTGTCGTGCTCGATCCGTGGGCGCTCAAGCAA
GAGGTCGAGAAGCTCGAGGGGCAGGGCGTCACGATCAACGACGACAATTTCGCCATCGCCGACAATTGCCCGCTGATCCT
GCCGATCCACCGCGATCTCGACGGCCTGCGTGAAGCTGCAGCCGGTACCGGCAAGATCGGCACCACCGGTCGCGGCATCG
GCCCGGCTTATGAAGACAAGGTCGGCCGCCGCGCGATCCGCGTGTGCGACCTCGAACATCTCGACAGCCTGGAGCCGCAG
CTCGACCGCCTCTGCGCCCATCACGATGCGCTGCGCGCCGGGTTCGAGGAGCCGCCCGTGGATCGGGAGCGGCTATTGGG
CGACCTTCGCGAAATCGCGCCCTTCGTGCTCCGCTTTGCGCAGCCGGTGTGGAAGCGGCTGAAGAAAGTGCGCAAGGCGG
GCGCGAAGATCCTCTTTGAAGGCGCGCAGGGCGTGCTGCTCGATGTCGATCACGGCACCTATCCCTTCGTCACCAGTTCC
AACACCGTCAGCGGCACGGCAGCGAGCGGCAGCGGATTAGGGCCAGGGGCGGCGGGCTTCGTCCTCGGCATCGTCAAGGC
CTATACCACCCGCGTCGGCAGCGGCCCTTTCCCGACCGAACTCGACGATGAAATCGGCCAGCGGCTGGGTGAGCGAGGGC
ATGAATTCGGCACCGTCACTGGTCGCCAGCGCCGGGTCGGATGGTTCGATGCCGTGCTGGTGCGCCAGACCTGCGCGATC
AGTGGCGTAACCGGTATCGCGCTGACCAAGATCGACGTGCTTGATGGGCTGGATACGGTGAAGATCTGCACGGGCTACCG
GCTTAACAATAATGTCTACGATTACCTGCCGAGCCATGCCGCGAGCCAGGCGGCGGTGGAACCGATCTACGAGGAAATGG
AAGGCTGGAGCGAAAGCACTGCCGGCGCGCGGAGCTTTGCCGACCTGCCCGCCAATGCGGTGAAATACATCCAGCGCATC
CAGGAACTGATCGAATGCCCAGTGGCGCTGGTCTCGACCAGCCCCGAGCGTGACGACACCATTTTGATGCGCGATCCGTT
TATGGATTGA

Upstream 100 bases:

>100_bases
GCACCGTATCCGGAACGCTCCGTCGTTCCTCCATCCCATCCCCTGGTCGAGTCCTGTCGAAGGACCTTGTGGATCCCCCG
GCAGGGGAAGAGGCATACCC

Downstream 100 bases:

>100_bases
TCAGGCGTCGCTGCCGACCGCCGCCAGCGCGGTTTCCTTAGCCCAGGCATAGTCGGCCTTGCCATTCGCGGCGCGGGCCA
CTTCGTCGACCACGACCAAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MANVTVIGAQWGDEGKGKIVDWLADRADAVVRFQGGHNAGHTLVVDGTTYKLSLLPSGIVSGTLSIIGNGVVLDPWALKQ
EVEKLEGQGVTINDDNFAIADNCPLILPIHRDLDGLREAAAGTGKIGTTGRGIGPAYEDKVGRRAIRVCDLEHLDSLEPQ
LDRLCAHHDALRAGFEEPPVDRERLLGDLREIAPFVLRFAQPVWKRLKKVRKAGAKILFEGAQGVLLDVDHGTYPFVTSS
NTVSGTAASGSGLGPGAAGFVLGIVKAYTTRVGSGPFPTELDDEIGQRLGERGHEFGTVTGRQRRVGWFDAVLVRQTCAI
SGVTGIALTKIDVLDGLDTVKICTGYRLNNNVYDYLPSHAASQAAVEPIYEEMEGWSESTAGARSFADLPANAVKYIQRI
QELIECPVALVSTSPERDDTILMRDPFMD

Sequences:

>Translated_429_residues
MANVTVIGAQWGDEGKGKIVDWLADRADAVVRFQGGHNAGHTLVVDGTTYKLSLLPSGIVSGTLSIIGNGVVLDPWALKQ
EVEKLEGQGVTINDDNFAIADNCPLILPIHRDLDGLREAAAGTGKIGTTGRGIGPAYEDKVGRRAIRVCDLEHLDSLEPQ
LDRLCAHHDALRAGFEEPPVDRERLLGDLREIAPFVLRFAQPVWKRLKKVRKAGAKILFEGAQGVLLDVDHGTYPFVTSS
NTVSGTAASGSGLGPGAAGFVLGIVKAYTTRVGSGPFPTELDDEIGQRLGERGHEFGTVTGRQRRVGWFDAVLVRQTCAI
SGVTGIALTKIDVLDGLDTVKICTGYRLNNNVYDYLPSHAASQAAVEPIYEEMEGWSESTAGARSFADLPANAVKYIQRI
QELIECPVALVSTSPERDDTILMRDPFMD
>Mature_428_residues
ANVTVIGAQWGDEGKGKIVDWLADRADAVVRFQGGHNAGHTLVVDGTTYKLSLLPSGIVSGTLSIIGNGVVLDPWALKQE
VEKLEGQGVTINDDNFAIADNCPLILPIHRDLDGLREAAAGTGKIGTTGRGIGPAYEDKVGRRAIRVCDLEHLDSLEPQL
DRLCAHHDALRAGFEEPPVDRERLLGDLREIAPFVLRFAQPVWKRLKKVRKAGAKILFEGAQGVLLDVDHGTYPFVTSSN
TVSGTAASGSGLGPGAAGFVLGIVKAYTTRVGSGPFPTELDDEIGQRLGERGHEFGTVTGRQRRVGWFDAVLVRQTCAIS
GVTGIALTKIDVLDGLDTVKICTGYRLNNNVYDYLPSHAASQAAVEPIYEEMEGWSESTAGARSFADLPANAVKYIQRIQ
ELIECPVALVSTSPERDDTILMRDPFMD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=43.7939110070258, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=40.8878504672897, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=41.8546365914787, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=50.9345794392523, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=41.92037470726, Blast_Score=327, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=41.6279069767442, Blast_Score=326, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=38.4259259259259, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=41.7661097852029, Blast_Score=308, Evalue=4e-84,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ERYLH (Q2NCV0)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457288.1
- ProteinModelPortal:   Q2NCV0
- SMR:   Q2NCV0
- STRING:   Q2NCV0
- GeneID:   3869580
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01995
- NMPDR:   fig|314225.3.peg.2690
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVQPMK
- PhylomeDB:   Q2NCV0
- ProtClustDB:   PRK01117
- BioCyc:   ELIT314225:ELI_01995-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46152; Mature: 46021

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVTVIGAQWGDEGKGKIVDWLADRADAVVRFQGGHNAGHTLVVDGTTYKLSLLPSGIV
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEECCCHHH
SGTLSIIGNGVVLDPWALKQEVEKLEGQGVTINDDNFAIADNCPLILPIHRDLDGLREAA
HHHHHHHCCCEEECHHHHHHHHHHHCCCCEEECCCCEEEECCCCEEEEECCCHHHHHHHH
AGTGKIGTTGRGIGPAYEDKVGRRAIRVCDLEHLDSLEPQLDRLCAHHDALRAGFEEPPV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
DRERLLGDLREIAPFVLRFAQPVWKRLKKVRKAGAKILFEGAQGVLLDVDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEECC
NTVSGTAASGSGLGPGAAGFVLGIVKAYTTRVGSGPFPTELDDEIGQRLGERGHEFGTVT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GRQRRVGWFDAVLVRQTCAISGVTGIALTKIDVLDGLDTVKICTGYRLNNNVYDYLPSHA
CCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCCEEHEECEEECCCHHHHCCCHH
ASQAAVEPIYEEMEGWSESTAGARSFADLPANAVKYIQRIQELIECPVALVSTSPERDDT
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCE
ILMRDPFMD
EEECCCCCC
>Mature Secondary Structure 
ANVTVIGAQWGDEGKGKIVDWLADRADAVVRFQGGHNAGHTLVVDGTTYKLSLLPSGIV
CCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEECCCHHH
SGTLSIIGNGVVLDPWALKQEVEKLEGQGVTINDDNFAIADNCPLILPIHRDLDGLREAA
HHHHHHHCCCEEECHHHHHHHHHHHCCCCEEECCCCEEEECCCCEEEEECCCHHHHHHHH
AGTGKIGTTGRGIGPAYEDKVGRRAIRVCDLEHLDSLEPQLDRLCAHHDALRAGFEEPPV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
DRERLLGDLREIAPFVLRFAQPVWKRLKKVRKAGAKILFEGAQGVLLDVDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEECC
NTVSGTAASGSGLGPGAAGFVLGIVKAYTTRVGSGPFPTELDDEIGQRLGERGHEFGTVT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GRQRRVGWFDAVLVRQTCAISGVTGIALTKIDVLDGLDTVKICTGYRLNNNVYDYLPSHA
CCCCCCHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCCEEHEECEEECCCHHHHCCCHH
ASQAAVEPIYEEMEGWSESTAGARSFADLPANAVKYIQRIQELIECPVALVSTSPERDDT
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCE
ILMRDPFMD
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA