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The map label for this gene is murD [H]

Identifier: 85373184

GI number: 85373184

Start: 400416

End: 401795

Strand: Direct

Name: murD [H]

Synonym: ELI_01785

Alternate gene names: 85373184

Gene position: 400416-401795 (Clockwise)

Preceding gene: 85373183

Following gene: 85373185

Centisome position: 13.12

GC content: 66.01

Gene sequence:

>1380_bases
ATGATCACCTGCCCCGCCTGGTCCGGCAAGAGTTTTGCCGTCCTTGGCCTGGCCCGCTCGGGCCAGGCGGCAGCAGAGGC
GCTGCTGGCGAGCGGGGCGGACGTCATGGTGTGGGACCGCCAGGAGGAGCCGCGCGCCGCCTTCGAAGGCCGCGCCCGCG
TCGGCGACCCGATGAGCGAAGACCTGACCGGTATCGACGGGCTGGTCGTCTCACCCGGAGTGCCGCTCAATACGCATCCC
ATCGTCGAGCGGGCCGGACATTTCGGCGTGCCGATCATCGGCGACATCGAACTCTTCGCGCAGGCGCGCAGCGCGCTGCC
GCCGCACCGGGTAATCGGCATCACCGGGACGAACGGAAAGTCGACCACCACCGCGTTGGTTACGCACCTGCTGAAAGAGG
CGGGCGTCGAAGCGCGCATGGGCGGCAATATCGGCCTGCCGATCCTCGGTGAAGAGCCCTTGCCGGAAGGCGGCGTCTAT
GTGCTCGAGCTTTCCAGCTACCAGATCGACCTCACACGTTCGCTTGCCTGCGAAGCGGCGGCGCTGCTCAACATCACGCC
CGACCATCTCGATCGTTATGACGGTTTCGAGTCTTATGCCTTTTCCAAGGGCAGGCTCTTCACCATGCAAGGCAGCGGCC
AATTCGCCTGCTTCGGCTGCGAGGATGCACAGACCCGTGCCGTCTGCAATGCCGCGCAATCGCGCCGCGCGGAGGGACGG
GCGATTTGTCTCGAGCCGGAGCAATACGCTGCGATGCAGGGCGACTGGCCCGCGCTGCAGGGCCCGCACAATCTGCAAAA
TGTCGCGGCGGCCATCGCGCTGGTCGAGGAAATGGGCGTGACGCGCGGCGACTGGGAAGCGGGCCTCACATCCTATTCCG
GCCTGCCGCACCGCATGGAGGTCGTCTGCGAGCATGAGAGCGTGCTGTTCGTCAATGACAGCAAGGCGACCAATCCCGCA
TCGACCGCGCCCGCCCTGGCCGCATGGCCTGCCGATCCGGAGCCGCGCGTGCATTGGATCGTCGGCGGTCTCGCCAAGGA
AGATGGGCTCGGCGAATGCGAAGCGCAGCTTGGCAATATCAAGCGCGCCTATGCGATCGGAGAAGCGGGGCCGCGTTTCG
CCGAGCTGCTCGGGGGGCGCGTGCCCGTGGAGCGGAGCGAATTGATTTGCGAGGCGGTCAAGCAGGCGGTTTCCAATGCT
GAAGCGGGCGACATCGTCCTGCTTTCTCCGGCCTGCGCCAGCTTCGACCAGTTCCGTGACTTCGAGAAGCGCGGCGAGCA
CTTCCGCCAGTTGGTCGGCGCGCTCTCGGGCTGCGACATCGATGATATTTGCGAGCCGACGCTGGCAGGCAATGGCGACA
TGAAGGGTGAAGCGGCATGA

Upstream 100 bases:

>100_bases
ATTTCGAACAGCTCGGCTGGAGCGAGAGCAAGGTCGTGATCCGCTTCTGGATCGTGTCCATCGTGCTCGCGCTGATGGGT
CTTGCCACACTGAAGCTGCG

Downstream 100 bases:

>100_bases
ATTCAACGCAGCAGCCCTATGTGCCCGGTGTCGGCGAGCGCATGGCGGGGGCGGAGCTACGCTCATCCTCGGCGATGTCC
GAGCTGCGGATCTGGTGGCG

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MITCPAWSGKSFAVLGLARSGQAAAEALLASGADVMVWDRQEEPRAAFEGRARVGDPMSEDLTGIDGLVVSPGVPLNTHP
IVERAGHFGVPIIGDIELFAQARSALPPHRVIGITGTNGKSTTTALVTHLLKEAGVEARMGGNIGLPILGEEPLPEGGVY
VLELSSYQIDLTRSLACEAAALLNITPDHLDRYDGFESYAFSKGRLFTMQGSGQFACFGCEDAQTRAVCNAAQSRRAEGR
AICLEPEQYAAMQGDWPALQGPHNLQNVAAAIALVEEMGVTRGDWEAGLTSYSGLPHRMEVVCEHESVLFVNDSKATNPA
STAPALAAWPADPEPRVHWIVGGLAKEDGLGECEAQLGNIKRAYAIGEAGPRFAELLGGRVPVERSELICEAVKQAVSNA
EAGDIVLLSPACASFDQFRDFEKRGEHFRQLVGALSGCDIDDICEPTLAGNGDMKGEAA

Sequences:

>Translated_459_residues
MITCPAWSGKSFAVLGLARSGQAAAEALLASGADVMVWDRQEEPRAAFEGRARVGDPMSEDLTGIDGLVVSPGVPLNTHP
IVERAGHFGVPIIGDIELFAQARSALPPHRVIGITGTNGKSTTTALVTHLLKEAGVEARMGGNIGLPILGEEPLPEGGVY
VLELSSYQIDLTRSLACEAAALLNITPDHLDRYDGFESYAFSKGRLFTMQGSGQFACFGCEDAQTRAVCNAAQSRRAEGR
AICLEPEQYAAMQGDWPALQGPHNLQNVAAAIALVEEMGVTRGDWEAGLTSYSGLPHRMEVVCEHESVLFVNDSKATNPA
STAPALAAWPADPEPRVHWIVGGLAKEDGLGECEAQLGNIKRAYAIGEAGPRFAELLGGRVPVERSELICEAVKQAVSNA
EAGDIVLLSPACASFDQFRDFEKRGEHFRQLVGALSGCDIDDICEPTLAGNGDMKGEAA
>Mature_459_residues
MITCPAWSGKSFAVLGLARSGQAAAEALLASGADVMVWDRQEEPRAAFEGRARVGDPMSEDLTGIDGLVVSPGVPLNTHP
IVERAGHFGVPIIGDIELFAQARSALPPHRVIGITGTNGKSTTTALVTHLLKEAGVEARMGGNIGLPILGEEPLPEGGVY
VLELSSYQIDLTRSLACEAAALLNITPDHLDRYDGFESYAFSKGRLFTMQGSGQFACFGCEDAQTRAVCNAAQSRRAEGR
AICLEPEQYAAMQGDWPALQGPHNLQNVAAAIALVEEMGVTRGDWEAGLTSYSGLPHRMEVVCEHESVLFVNDSKATNPA
STAPALAAWPADPEPRVHWIVGGLAKEDGLGECEAQLGNIKRAYAIGEAGPRFAELLGGRVPVERSELICEAVKQAVSNA
EAGDIVLLSPACASFDQFRDFEKRGEHFRQLVGALSGCDIDDICEPTLAGNGDMKGEAA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=450, Percent_Identity=36.6666666666667, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48604; Mature: 48604

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITCPAWSGKSFAVLGLARSGQAAAEALLASGADVMVWDRQEEPRAAFEGRARVGDPMSE
CEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCCCHHH
DLTGIDGLVVSPGVPLNTHPIVERAGHFGVPIIGDIELFAQARSALPPHRVIGITGTNGK
HHCCCCCEEECCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCC
STTTALVTHLLKEAGVEARMGGNIGLPILGEEPLPEGGVYVLELSSYQIDLTRSLACEAA
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHH
ALLNITPDHLDRYDGFESYAFSKGRLFTMQGSGQFACFGCEDAQTRAVCNAAQSRRAEGR
HHCCCCHHHHHHHCCHHHHEECCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCC
AICLEPEQYAAMQGDWPALQGPHNLQNVAAAIALVEEMGVTRGDWEAGLTSYSGLPHRME
EEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHCCCCCHHHH
VVCEHESVLFVNDSKATNPASTAPALAAWPADPEPRVHWIVGGLAKEDGLGECEAQLGNI
EEECCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHCCC
KRAYAIGEAGPRFAELLGGRVPVERSELICEAVKQAVSNAEAGDIVLLSPACASFDQFRD
HHEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHCHHHHHH
FEKRGEHFRQLVGALSGCDIDDICEPTLAGNGDMKGEAA
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITCPAWSGKSFAVLGLARSGQAAAEALLASGADVMVWDRQEEPRAAFEGRARVGDPMSE
CEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCCCHHH
DLTGIDGLVVSPGVPLNTHPIVERAGHFGVPIIGDIELFAQARSALPPHRVIGITGTNGK
HHCCCCCEEECCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCC
STTTALVTHLLKEAGVEARMGGNIGLPILGEEPLPEGGVYVLELSSYQIDLTRSLACEAA
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHH
ALLNITPDHLDRYDGFESYAFSKGRLFTMQGSGQFACFGCEDAQTRAVCNAAQSRRAEGR
HHCCCCHHHHHHHCCHHHHEECCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCC
AICLEPEQYAAMQGDWPALQGPHNLQNVAAAIALVEEMGVTRGDWEAGLTSYSGLPHRME
EEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHCCCCCHHHH
VVCEHESVLFVNDSKATNPASTAPALAAWPADPEPRVHWIVGGLAKEDGLGECEAQLGNI
EEECCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHCCC
KRAYAIGEAGPRFAELLGGRVPVERSELICEAVKQAVSNAEAGDIVLLSPACASFDQFRD
HHEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHCHHHHHH
FEKRGEHFRQLVGALSGCDIDDICEPTLAGNGDMKGEAA
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA