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The map label for this gene is dapF [H]

Identifier: 84625424

GI number: 84625424

Start: 4259765

End: 4260619

Strand: Reverse

Name: dapF [H]

Synonym: XOO_3767

Alternate gene names: 84625424

Gene position: 4260619-4259765 (Counterclockwise)

Preceding gene: 84625425

Following gene: 84625423

Centisome position: 86.24

GC content: 67.13

Gene sequence:

>855_bases
ATGAGCGCTGACGGTCGCAGCGGGCGTCTGCGTTTCACCAAAATGCACGGCGCCGGCAACGATTTCGTGGTACTGGACCT
GCGCGACGGCACGCCGCCGCCAGATGCAGCGCTGGCAGCGCAATTGGCCGACCGCCACTTCGGGGTGGGTTGCGATCAGA
TCCTGACCATCGAAGCGCCGCGCAGCGAAGGCGCCGTGGCGGCCTACGGCATCTGGAATTCCGACGGCTCGGCAGCGCGC
CAATGCGGCAATGGCGCGCGCTGTGTCGCCGCCTGGCTGGTGCGCGATGGCATCGCCCAGTGCGAGCGTTTCATCATCGA
TAGCCCCGTCACCGCGCATGCAGTCGAGCGCCTTGAAGGCGGCCGCTACGCGGTGGCGATGGGTATGCCGCAGTTCGACC
CCCCCCAGATTCCGCTGGCTGGATTCGCGCATGCACGCGACGAATATGCGCTGCCGGTGCACGGCGATACCGTGCGCTTT
GGTGCGGTGTCGATGGGCAACCCGCATGCGGTGGTCGAAGTCGGTCGCGTGGATGCGGCACCGGTCGAACGCGTCGGTAC
GCTGCTGCAGCAAAACGCCGCGTTTCCCGATTCGGTCAATGTGGGTTTCGTGCAGGTGGTCGACCCCACGCATGTGCGCT
TGCGCGTGTTCGAGCGCGGGGTGGGGGAAACGCTGGCCTGCGGCAGCGGTGCCTGTGCGGCCGCGGTGGTGCTGATGCAG
CGCTGCCGCGTCGAGCGCGACGTGCAGGTGTCCTTGCCGGGTGGCGAGCTACGCATTCGTTGGCCCTGTGACCACGAGCA
AGTGGTCATGTCCGGCCCGGCGGTGTTCGTCTTCGATGGAGAGTGGAACGGATGA

Upstream 100 bases:

>100_bases
CCCGTGCCGCAGCCGGTGGAAGAGTCCACGCCTGCGCCCGTCCCCGCGGACAGCAGCACCAAGCCCGCCCCCACCACAGA
CCAGCCCGCTAGCAGCGGCA

Downstream 100 bases:

>100_bases
GCGAGAACACGGACAAATTCGGCGCACATGAAGTCGCCACCTGGCTGCGACGTCACCCGACCTTCCTCAAGCAGTTCCCG
GATCTGGCAGTGAGCTTGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSADGRSGRLRFTKMHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGVGCDQILTIEAPRSEGAVAAYGIWNSDGSAAR
QCGNGARCVAAWLVRDGIAQCERFIIDSPVTAHAVERLEGGRYAVAMGMPQFDPPQIPLAGFAHARDEYALPVHGDTVRF
GAVSMGNPHAVVEVGRVDAAPVERVGTLLQQNAAFPDSVNVGFVQVVDPTHVRLRVFERGVGETLACGSGACAAAVVLMQ
RCRVERDVQVSLPGGELRIRWPCDHEQVVMSGPAVFVFDGEWNG

Sequences:

>Translated_284_residues
MSADGRSGRLRFTKMHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGVGCDQILTIEAPRSEGAVAAYGIWNSDGSAAR
QCGNGARCVAAWLVRDGIAQCERFIIDSPVTAHAVERLEGGRYAVAMGMPQFDPPQIPLAGFAHARDEYALPVHGDTVRF
GAVSMGNPHAVVEVGRVDAAPVERVGTLLQQNAAFPDSVNVGFVQVVDPTHVRLRVFERGVGETLACGSGACAAAVVLMQ
RCRVERDVQVSLPGGELRIRWPCDHEQVVMSGPAVFVFDGEWNG
>Mature_283_residues
SADGRSGRLRFTKMHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGVGCDQILTIEAPRSEGAVAAYGIWNSDGSAARQ
CGNGARCVAAWLVRDGIAQCERFIIDSPVTAHAVERLEGGRYAVAMGMPQFDPPQIPLAGFAHARDEYALPVHGDTVRFG
AVSMGNPHAVVEVGRVDAAPVERVGTLLQQNAAFPDSVNVGFVQVVDPTHVRLRVFERGVGETLACGSGACAAAVVLMQR
CRVERDVQVSLPGGELRIRWPCDHEQVVMSGPAVFVFDGEWNG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=271, Percent_Identity=42.4354243542435, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30239; Mature: 30107

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADGRSGRLRFTKMHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGVGCDQILTIEAP
CCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
RSEGAVAAYGIWNSDGSAARQCGNGARCVAAWLVRDGIAQCERFIIDSPVTAHAVERLEG
CCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
GRYAVAMGMPQFDPPQIPLAGFAHARDEYALPVHGDTVRFGAVSMGNPHAVVEVGRVDAA
CCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCEEEEEECCCCCC
PVERVGTLLQQNAAFPDSVNVGFVQVVDPTHVRLRVFERGVGETLACGSGACAAAVVLMQ
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEECCCCCHHHHHHHHHH
RCRVERDVQVSLPGGELRIRWPCDHEQVVMSGPAVFVFDGEWNG
HHCCCCCEEEECCCCEEEEECCCCCCEEEEECCEEEEECCCCCC
>Mature Secondary Structure 
SADGRSGRLRFTKMHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGVGCDQILTIEAP
CCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
RSEGAVAAYGIWNSDGSAARQCGNGARCVAAWLVRDGIAQCERFIIDSPVTAHAVERLEG
CCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
GRYAVAMGMPQFDPPQIPLAGFAHARDEYALPVHGDTVRFGAVSMGNPHAVVEVGRVDAA
CCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCEEEEEECCCCCC
PVERVGTLLQQNAAFPDSVNVGFVQVVDPTHVRLRVFERGVGETLACGSGACAAAVVLMQ
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEECCCCCHHHHHHHHHH
RCRVERDVQVSLPGGELRIRWPCDHEQVVMSGPAVFVFDGEWNG
HHCCCCCEEEECCCCEEEEECCCCCCEEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]