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The map label for this gene is dut [H]

Identifier: 84622121

GI number: 84622121

Start: 502937

End: 503404

Strand: Direct

Name: dut [H]

Synonym: XOO_0464

Alternate gene names: 84622121

Gene position: 502937-503404 (Clockwise)

Preceding gene: 84622120

Following gene: 84622122

Centisome position: 10.18

GC content: 68.38

Gene sequence:

>468_bases
ATGAGCACCCCGACCCAGTCCTTGCAGGTGAAGTTGCTCGACCCGCGCTTCGGCGATCTGTGGCCGTTGCCGGCCTATGC
CACCGAATCCAGTGCAGGCATGGACCTGCGCGCCGCGCTGGAGGCGCCGATGACGCTGCAGCCGGGCGATGCGGCGCTGA
TCCCCAGCGGCATCGCGATCCATCTGGCCGACCCGCAGCTGTGCGCGGTGATCCTGCCGCGCTCGGGGTTGGGCCATCGC
CACGGCATCGTGCTCGGCAACGGTACCGGCCTCATCGATGCGGATTACCAGGGGCCGTTGCTGATCAGCACCTGGAATCG
CGGCCGCGAGGCCTTCACCATCGAGCCGGGCGACCGCATTGCGCAGCTGGTGATCCTGCCGATCGTGCGCGCGGGCTTGC
AAGTGGTGGATACTTTCGTTGACAGCGCGCGGGGAGCGGGTGGATTCGGCCACACCGGCGTGCGCTGA

Upstream 100 bases:

>100_bases
CCACTGCCTATTGGCCGGGCGGCGAGCGCGCGTTTCCCAGTAGCAGCAAGACTGAATTGGCCGACCAACTACTGGCCTTG
ATTGCGGAGAGATTGCAGGC

Downstream 100 bases:

>100_bases
CTGGGGAAATCACATGAGCAACGCGAACGAGCGCAGGCAGTCGATGTCCAGCGTGCGTATGAGTGGTCTGGTGTTGGCGG
GCATATTGCTGTTGCTGGCC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSTPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAIHLADPQLCAVILPRSGLGHR
HGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRIAQLVILPIVRAGLQVVDTFVDSARGAGGFGHTGVR

Sequences:

>Translated_155_residues
MSTPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAIHLADPQLCAVILPRSGLGHR
HGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRIAQLVILPIVRAGLQVVDTFVDSARGAGGFGHTGVR
>Mature_154_residues
STPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAIHLADPQLCAVILPRSGLGHRH
GIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRIAQLVILPIVRAGLQVVDTFVDSARGAGGFGHTGVR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI4503423, Length=151, Percent_Identity=35.0993377483444, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=35.8620689655172, Blast_Score=83, Evalue=8e-17,
Organism=Homo sapiens, GI70906441, Length=151, Percent_Identity=35.0993377483444, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=59.4594594594595, Blast_Score=195, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71988561, Length=150, Percent_Identity=34.6666666666667, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319729, Length=84, Percent_Identity=47.6190476190476, Blast_Score=75, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921126, Length=132, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583610, Length=132, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16298; Mature: 16167

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAI
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCEECCCCCEEECCCEEE
HLADPQLCAVILPRSGLGHRHGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRI
EECCCCEEEEEECCCCCCCCCCEEEECCCEEEECCCCCCEEEEECCCCCEEEEECCCHHH
AQLVILPIVRAGLQVVDTFVDSARGAGGFGHTGVR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAI
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCEECCCCCEEECCCEEE
HLADPQLCAVILPRSGLGHRHGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRI
EECCCCEEEEEECCCCCCCCCCEEEECCCEEEECCCCCCEEEEECCCCCEEEEECCCHHH
AQLVILPIVRAGLQVVDTFVDSARGAGGFGHTGVR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]