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The map label for this gene is hisA

Identifier: 84489201

GI number: 84489201

Start: 452662

End: 453375

Strand: Direct

Name: hisA

Synonym: Msp_0389

Alternate gene names: 84489201

Gene position: 452662-453375 (Clockwise)

Preceding gene: 84489197

Following gene: 84489202

Centisome position: 25.61

GC content: 29.41

Gene sequence:

>714_bases
ATGATTATAATACCAGCAGTAGATATTAAAAATGGGAAATGTGTACAGTTAGTACAAGGAGAACCTGGAACAGAACAAGT
AGTAATTGAAAATCCTGCAGAAGTAGCAAAACAATGGGAACAAAAGGGAGCAAAAAAATTACATATTGTTGATTTAGATG
GTGCACTAGGTAGTGGAAGAAATCTTTCAATAGTCAAAGAAATAATAGAAAAAACAAAGTCTCCTATTCAATTGGGTGGA
GGAATAAGATCCGTTAGTGATGCAAAGAAATTACTTGAAATTGGAGTTAACACTGTAATAATAGGTACAATGGCAATTAA
AAATCCAGAAATAATCAAGGAATTATCTAGTGAATATGGTTGTGAAAGAATCTGTGTTTCTTTAGATAGTAAAAACAACA
AAGTAGTAACACATGGCTGGAAAGAATCTACAGATAAAACACCAATTGAATATGCTAAATTATTTGAAAAAATGGGTGCA
GGTTCAATACTATTTACAAATGTGGATGTTGAAGGGTTATTAAATGGAATAAATCTTGAACCTATAAAAGAATTATTAAA
TAATGTCTCTATTCCAATAATATATTCTGGTGGTATAACTTCTGTTGATGATTTAAAAGTTTTATCAGATATTGGAGTAG
ATTATGTTGTAATTGGTTCTGCATTATACAAAGGATTAATAACATTAGAAGATGCTTTAAAATATCAAAAATAG

Upstream 100 bases:

>100_bases
TAAGTTTTTTATCAACATATTAAACGTTAATAACATAATATATTAAACATATAAATTTAAAATAATTAGAACTTTTTTAT
AAAAAAATGAGTTGAATATA

Downstream 100 bases:

>100_bases
ATTAGGGGGTATTTAAATTAAAGTTATGGCTTCAGGAACATTTGATATAATACATCCAGGTCATGGTTTTTACTTATCAG
AATCAAAAAAACTTGGTGGA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIIIPAVDIKNGKCVQLVQGEPGTEQVVIENPAEVAKQWEQKGAKKLHIVDLDGALGSGRNLSIVKEIIEKTKSPIQLGG
GIRSVSDAKKLLEIGVNTVIIGTMAIKNPEIIKELSSEYGCERICVSLDSKNNKVVTHGWKESTDKTPIEYAKLFEKMGA
GSILFTNVDVEGLLNGINLEPIKELLNNVSIPIIYSGGITSVDDLKVLSDIGVDYVVIGSALYKGLITLEDALKYQK

Sequences:

>Translated_237_residues
MIIIPAVDIKNGKCVQLVQGEPGTEQVVIENPAEVAKQWEQKGAKKLHIVDLDGALGSGRNLSIVKEIIEKTKSPIQLGG
GIRSVSDAKKLLEIGVNTVIIGTMAIKNPEIIKELSSEYGCERICVSLDSKNNKVVTHGWKESTDKTPIEYAKLFEKMGA
GSILFTNVDVEGLLNGINLEPIKELLNNVSIPIIYSGGITSVDDLKVLSDIGVDYVVIGSALYKGLITLEDALKYQK
>Mature_237_residues
MIIIPAVDIKNGKCVQLVQGEPGTEQVVIENPAEVAKQWEQKGAKKLHIVDLDGALGSGRNLSIVKEIIEKTKSPIQLGG
GIRSVSDAKKLLEIGVNTVIIGTMAIKNPEIIKELSSEYGCERICVSLDSKNNKVVTHGWKESTDKTPIEYAKLFEKMGA
GSILFTNVDVEGLLNGINLEPIKELLNNVSIPIIYSGGITSVDDLKVLSDIGVDYVVIGSALYKGLITLEDALKYQK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=30.5439330543933, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788336, Length=246, Percent_Identity=26.8292682926829, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_METST (Q2NHB3)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447433.1
- HSSP:   P16250
- ProteinModelPortal:   Q2NHB3
- SMR:   Q2NHB3
- STRING:   Q2NHB3
- GeneID:   3855754
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0389
- NMPDR:   fig|339860.6.peg.371
- eggNOG:   arNOG08089
- HOGENOM:   HBG541613
- OMA:   CARYVVT
- PhylomeDB:   Q2NHB3
- ProtClustDB:   PRK13585
- BioCyc:   MSTA339860:MSP_0389-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25627; Mature: 25627

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIPAVDIKNGKCVQLVQGEPGTEQVVIENPAEVAKQWEQKGAKKLHIVDLDGALGSGR
CEEEEEEECCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCCC
NLSIVKEIIEKTKSPIQLGGGIRSVSDAKKLLEIGVNTVIIGTMAIKNPEIIKELSSEYG
CHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCC
CERICVSLDSKNNKVVTHGWKESTDKTPIEYAKLFEKMGAGSILFTNVDVEGLLNGINLE
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCHH
PIKELLNNVSIPIIYSGGITSVDDLKVLSDIGVDYVVIGSALYKGLITLEDALKYQK
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIIIPAVDIKNGKCVQLVQGEPGTEQVVIENPAEVAKQWEQKGAKKLHIVDLDGALGSGR
CEEEEEEECCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCCC
NLSIVKEIIEKTKSPIQLGGGIRSVSDAKKLLEIGVNTVIIGTMAIKNPEIIKELSSEYG
CHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCC
CERICVSLDSKNNKVVTHGWKESTDKTPIEYAKLFEKMGAGSILFTNVDVEGLLNGINLE
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCHH
PIKELLNNVSIPIIYSGGITSVDDLKVLSDIGVDYVVIGSALYKGLITLEDALKYQK
HHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA