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The map label for this gene is rfaE [C]

Identifier: 84489202

GI number: 84489202

Start: 453401

End: 453847

Strand: Direct

Name: rfaE [C]

Synonym: Msp_0390

Alternate gene names: 84489202

Gene position: 453401-453847 (Clockwise)

Preceding gene: 84489201

Following gene: 84489203

Centisome position: 25.65

GC content: 28.86

Gene sequence:

>447_bases
ATGGCTTCAGGAACATTTGATATAATACATCCAGGTCATGGTTTTTACTTATCAGAATCAAAAAAACTTGGTGGAGATAA
TGCAATTTTAATGGTTGTTGTTGCAACAGATAAAACAGTTAAAAATCATAAACGAGTACCTATTGTGGGTGAAAAACAAC
GGTGTGAAATGGTATCTATGTTAAAAGGTGTTGATGAAGCATATGTTGGGGATGAAAAAGATCCTTTTAAAATAGTTAAA
GAAAAAAAGCCAGACATAATAACGATAGGTCCTGATCAAAACTTCAATCCAGACTCATTACACAAATCTCTTCTAAAAAA
GGGATTAGACATAAAAGTTGTTAAAATAAATGATTATAAAAAATTTGAATTAGATAGTAGTTGTAAAATTATACGTAAAA
TAAAACAAACAGATTTTGATATGGATTATTTGGAAAATTGCCAATAG

Upstream 100 bases:

>100_bases
GATTATGTTGTAATTGGTTCTGCATTATACAAAGGATTAATAACATTAGAAGATGCTTTAAAATATCAAAAATAGATTAG
GGGGTATTTAAATTAAAGTT

Downstream 100 bases:

>100_bases
TGAATATATAAAATTTAATGAGTATATTGATGTTATTTCTTTTAATATGTCATTTAGGTGGATATGGGAATAGAATATTT
TTTGATTATTTGGGTATATT

Product: putative cytidylyltransferase

Products: diphosphate; CDPglycerol

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MASGTFDIIHPGHGFYLSESKKLGGDNAILMVVVATDKTVKNHKRVPIVGEKQRCEMVSMLKGVDEAYVGDEKDPFKIVK
EKKPDIITIGPDQNFNPDSLHKSLLKKGLDIKVVKINDYKKFELDSSCKIIRKIKQTDFDMDYLENCQ

Sequences:

>Translated_148_residues
MASGTFDIIHPGHGFYLSESKKLGGDNAILMVVVATDKTVKNHKRVPIVGEKQRCEMVSMLKGVDEAYVGDEKDPFKIVK
EKKPDIITIGPDQNFNPDSLHKSLLKKGLDIKVVKINDYKKFELDSSCKIIRKIKQTDFDMDYLENCQ
>Mature_147_residues
ASGTFDIIHPGHGFYLSESKKLGGDNAILMVVVATDKTVKNHKRVPIVGEKQRCEMVSMLKGVDEAYVGDEKDPFKIVKE
KKPDIITIGPDQNFNPDSLHKSLLKKGLDIKVVKINDYKKFELDSSCKIIRKIKQTDFDMDYLENCQ

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0615

COG function: function code MI; Cytidylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.39

Molecular weight: Translated: 16745; Mature: 16613

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGTFDIIHPGHGFYLSESKKLGGDNAILMVVVATDKTVKNHKRVPIVGEKQRCEMVSM
CCCCCEEEEECCCCEEEECCCCCCCCCEEEEEEEECCCCHHCCCCCCCCCCHHHHHHHHH
LKGVDEAYVGDEKDPFKIVKEKKPDIITIGPDQNFNPDSLHKSLLKKGLDIKVVKINDYK
HHCCCHHHCCCCCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCE
KFELDSSCKIIRKIKQTDFDMDYLENCQ
EECCCCHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure 
ASGTFDIIHPGHGFYLSESKKLGGDNAILMVVVATDKTVKNHKRVPIVGEKQRCEMVSM
CCCCEEEEECCCCEEEECCCCCCCCCEEEEEEEECCCCHHCCCCCCCCCCHHHHHHHHH
LKGVDEAYVGDEKDPFKIVKEKKPDIITIGPDQNFNPDSLHKSLLKKGLDIKVVKINDYK
HHCCCHHHCCCCCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCE
KFELDSSCKIIRKIKQTDFDMDYLENCQ
EECCCCHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]