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The map label for this gene is topA

Identifier: 84489180

GI number: 84489180

Start: 428436

End: 430604

Strand: Direct

Name: topA

Synonym: Msp_0366

Alternate gene names: 84489180

Gene position: 428436-430604 (Clockwise)

Preceding gene: 84489177

Following gene: 84489182

Centisome position: 24.24

GC content: 30.24

Gene sequence:

>2169_bases
ATGAGTGAATTAATTATTTGTGAAAAACCTAAAGTGGCTGAAAAAGTAGCTAAAGCTTTATCTGATTCACCAGTAAAAAA
TTCATATAAAAGAGTACCATATTATGAGTTTACAAATGGAAATGGAACAAAAATTACAGTATTATCTGCAGTGGGTCATT
TATTTTCATTAAAAGCAAAAAATAAAAAGGATAAACGTTTATTTGATGTTGAATGGGTACCTTTAAGTGAAACTGATAAA
TCAAAAAAATATGTTAAAAATTATATAGATACTATAAAGAAATTCTCAAAAGATGCAGATAGATTCATACATGCATGTGA
TTATGATACAGAAGGAACATTAATAGGATTTAATGCTCTAAGATATATCTGTGGAGAAGATAGTATTGATAAATCATTTA
GAATGAAATTTTCAGCTTTAACAAAAAAAGACTTAATAGAATCATATTCAAATGCATATCCATTAAAAGAAGATAAATCA
TGGGTAGATAGTGGAGAAACACGTCATGTTCTTGATTTCTTATTTGGAGTAAATATCTCAAAATCAATGACAGACTCTGT
GTTGAATGTAACAAATAGATATGTTCAATTATCTGCTGGACGTGTACAAACACCAACACTTGCAATACTAACAGAACGTG
AAAAGGAAATACAAAAGTTCATACCAGAACCTTACTGGCTAATAAAAGCAAAACTTCAAAAATCAATAGTAGCAGATCAT
AAGAAGGGTAAAATCTTTGATAAAAAGGAAGTAGATGCAATTCTTAAAAATTGTAAAGGAAAGGATGCTACAGTAGAAAA
AATAACCAATAGAAAAACTAAAAAAGATTTACCAGTACCATTTGAATTAGGAACATTACAATCAGAAGCATATGCACAAT
TTGGTTTTACTCCACGTAAAACACAACAAATAGCACAAAATCTCTATGTTGAGGGATACACATCCTATCCAAGAACATCA
TCACAGAAACTTCCAGAATCCTTAGGACTACCAAATATACTTAGTCAACTATCTAAACATCCAAAATATAAGGATAAGAT
AAGTCAACTAGAACAACCATACAAACCACATGAAGGTAAAAAAACTGATGAGGCACATCCTGCAATACATCCAACAGGAA
CATTACCTAAGGACATATCTGAGGATTATCAGAAGATTTATGATCTTATAACATATAGATTTATCAGTATATTTGGTAAA
CCTGCAGAAATGGAATCTATAAAAGTAGAATTAGATATTGGTGGAGAACCATTCTCATTTTCAAGACAAAGAATTTCTAA
GGAAGGATGGTTAAGTTTAGATCCATATCAGTATAAAAAAGTTAAAAATGAGGAATTCCCAGAGATAAAAGAGGGTCAAA
CAACTAAAGCTAAAGTAGCTAGTGAAGAAAAAGAAACAAAACCACCTGCAAGATATAATCAAGCATCAATTATAAGGGAA
CTTGAAAAAAGAGGTTTAGGAACAAAATCCACAAGGGCAAACATAGTTTCAATATTATATACTAGAAAATATGTTGAAGG
TAAAAAAATAGAAGTTAGTCAACTTGGTCAACAAATAATAAATACTCTAGAAAAATATTCTGAAAGAATTACTAGTGAAC
AAATGACTCGTGAATTTGAAACTGACATATCTAATATTAAGGAAAATGAAATTACAGAAGCAACTGTGATTGAAGATGCT
AAAAAAGAGTTAAATGGAATTCTTGATTCTATTGATGATAATATTGAAGATATTGGTAAGGAATTATATGGTGCATATGA
ACAAAGTCGTGTAGTTGGAAAATGTGGTTGTGGTGGTAACTTAATAATAATTTCATCACCACGTGGTGGAAAATTCGTTG
GATGTTCTAATTATCCAGATTGTAAGAAAACATATTCATTACCAGCAGGTGCTAATGTTCTTAAAACTACATGTGAAAAA
TGTGGACTACCACTAATTTCATATGGAAAACCTAGACAAAGAGCATGTCTAGATTTTGAATGTGCAAATGGTGGACAAAA
ATCAACAAATGATGTTGTTGGTGAATGTCCTGATTGTGGTAAAGATCTAATAAAAAGAATGGGTAGATTTGGTGAATTTA
TAGGATGTACTGGTTTTCCTAAGTGTAGATTCACATCATCCATCGATGACTTTGAGAAATCTAAGAAAGAATCAGAGAAG
AAAGATTAA

Upstream 100 bases:

>100_bases
AAATCTATACTATTTCTATTTGAATAAATTTTTTCTTGAAATAAAAGTGTTAAATATAATCTTTCATTATTATTAATTAA
ATAAAAAAAGGAGATAGAAT

Downstream 100 bases:

>100_bases
TATAAGAATATAATACTTAGAATGAATAAATTTTCTAGGTATTAACACTCCTTTTTTTTTAATTATTTGAAATAATTTCA
TATAAATGTTTTAGTATGAT

Product: hypothetical protein

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MSELIICEKPKVAEKVAKALSDSPVKNSYKRVPYYEFTNGNGTKITVLSAVGHLFSLKAKNKKDKRLFDVEWVPLSETDK
SKKYVKNYIDTIKKFSKDADRFIHACDYDTEGTLIGFNALRYICGEDSIDKSFRMKFSALTKKDLIESYSNAYPLKEDKS
WVDSGETRHVLDFLFGVNISKSMTDSVLNVTNRYVQLSAGRVQTPTLAILTEREKEIQKFIPEPYWLIKAKLQKSIVADH
KKGKIFDKKEVDAILKNCKGKDATVEKITNRKTKKDLPVPFELGTLQSEAYAQFGFTPRKTQQIAQNLYVEGYTSYPRTS
SQKLPESLGLPNILSQLSKHPKYKDKISQLEQPYKPHEGKKTDEAHPAIHPTGTLPKDISEDYQKIYDLITYRFISIFGK
PAEMESIKVELDIGGEPFSFSRQRISKEGWLSLDPYQYKKVKNEEFPEIKEGQTTKAKVASEEKETKPPARYNQASIIRE
LEKRGLGTKSTRANIVSILYTRKYVEGKKIEVSQLGQQIINTLEKYSERITSEQMTREFETDISNIKENEITEATVIEDA
KKELNGILDSIDDNIEDIGKELYGAYEQSRVVGKCGCGGNLIIISSPRGGKFVGCSNYPDCKKTYSLPAGANVLKTTCEK
CGLPLISYGKPRQRACLDFECANGGQKSTNDVVGECPDCGKDLIKRMGRFGEFIGCTGFPKCRFTSSIDDFEKSKKESEK
KD

Sequences:

>Translated_722_residues
MSELIICEKPKVAEKVAKALSDSPVKNSYKRVPYYEFTNGNGTKITVLSAVGHLFSLKAKNKKDKRLFDVEWVPLSETDK
SKKYVKNYIDTIKKFSKDADRFIHACDYDTEGTLIGFNALRYICGEDSIDKSFRMKFSALTKKDLIESYSNAYPLKEDKS
WVDSGETRHVLDFLFGVNISKSMTDSVLNVTNRYVQLSAGRVQTPTLAILTEREKEIQKFIPEPYWLIKAKLQKSIVADH
KKGKIFDKKEVDAILKNCKGKDATVEKITNRKTKKDLPVPFELGTLQSEAYAQFGFTPRKTQQIAQNLYVEGYTSYPRTS
SQKLPESLGLPNILSQLSKHPKYKDKISQLEQPYKPHEGKKTDEAHPAIHPTGTLPKDISEDYQKIYDLITYRFISIFGK
PAEMESIKVELDIGGEPFSFSRQRISKEGWLSLDPYQYKKVKNEEFPEIKEGQTTKAKVASEEKETKPPARYNQASIIRE
LEKRGLGTKSTRANIVSILYTRKYVEGKKIEVSQLGQQIINTLEKYSERITSEQMTREFETDISNIKENEITEATVIEDA
KKELNGILDSIDDNIEDIGKELYGAYEQSRVVGKCGCGGNLIIISSPRGGKFVGCSNYPDCKKTYSLPAGANVLKTTCEK
CGLPLISYGKPRQRACLDFECANGGQKSTNDVVGECPDCGKDLIKRMGRFGEFIGCTGFPKCRFTSSIDDFEKSKKESEK
KD
>Mature_721_residues
SELIICEKPKVAEKVAKALSDSPVKNSYKRVPYYEFTNGNGTKITVLSAVGHLFSLKAKNKKDKRLFDVEWVPLSETDKS
KKYVKNYIDTIKKFSKDADRFIHACDYDTEGTLIGFNALRYICGEDSIDKSFRMKFSALTKKDLIESYSNAYPLKEDKSW
VDSGETRHVLDFLFGVNISKSMTDSVLNVTNRYVQLSAGRVQTPTLAILTEREKEIQKFIPEPYWLIKAKLQKSIVADHK
KGKIFDKKEVDAILKNCKGKDATVEKITNRKTKKDLPVPFELGTLQSEAYAQFGFTPRKTQQIAQNLYVEGYTSYPRTSS
QKLPESLGLPNILSQLSKHPKYKDKISQLEQPYKPHEGKKTDEAHPAIHPTGTLPKDISEDYQKIYDLITYRFISIFGKP
AEMESIKVELDIGGEPFSFSRQRISKEGWLSLDPYQYKKVKNEEFPEIKEGQTTKAKVASEEKETKPPARYNQASIIREL
EKRGLGTKSTRANIVSILYTRKYVEGKKIEVSQLGQQIINTLEKYSERITSEQMTREFETDISNIKENEITEATVIEDAK
KELNGILDSIDDNIEDIGKELYGAYEQSRVVGKCGCGGNLIIISSPRGGKFVGCSNYPDCKKTYSLPAGANVLKTTCEKC
GLPLISYGKPRQRACLDFECANGGQKSTNDVVGECPDCGKDLIKRMGRFGEFIGCTGFPKCRFTSSIDDFEKSKKESEKK
D

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=674, Percent_Identity=24.4807121661721, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI10835218, Length=678, Percent_Identity=23.7463126843658, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI1787529, Length=771, Percent_Identity=26.8482490272374, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788061, Length=615, Percent_Identity=25.3658536585366, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17555378, Length=557, Percent_Identity=27.6481149012567, Blast_Score=185, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI32563869, Length=581, Percent_Identity=24.7848537005164, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323263, Length=636, Percent_Identity=23.2704402515723, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24585251, Length=699, Percent_Identity=24.6065808297568, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24640096, Length=664, Percent_Identity=25.1506024096386, Blast_Score=161, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81658; Mature: 81527

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIICEKPKVAEKVAKALSDSPVKNSYKRVPYYEFTNGNGTKITVLSAVGHLFSLKAK
CCCCEECCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHHHCC
NKKDKRLFDVEWVPLSETDKSKKYVKNYIDTIKKFSKDADRFIHACDYDTEGTLIGFNAL
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
RYICGEDSIDKSFRMKFSALTKKDLIESYSNAYPLKEDKSWVDSGETRHVLDFLFGVNIS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHCCCCC
KSMTDSVLNVTNRYVQLSAGRVQTPTLAILTEREKEIQKFIPEPYWLIKAKLQKSIVADH
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
KKGKIFDKKEVDAILKNCKGKDATVEKITNRKTKKDLPVPFELGTLQSEAYAQFGFTPRK
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHCCCCHHH
TQQIAQNLYVEGYTSYPRTSSQKLPESLGLPNILSQLSKHPKYKDKISQLEQPYKPHEGK
HHHHHHHHHHCCCCCCCCCCHHHCHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
KTDEAHPAIHPTGTLPKDISEDYQKIYDLITYRFISIFGKPAEMESIKVELDIGGEPFSF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHH
SRQRISKEGWLSLDPYQYKKVKNEEFPEIKEGQTTKAKVASEEKETKPPARYNQASIIRE
HHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
LEKRGLGTKSTRANIVSILYTRKYVEGKKIEVSQLGQQIINTLEKYSERITSEQMTREFE
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDISNIKENEITEATVIEDAKKELNGILDSIDDNIEDIGKELYGAYEQSRVVGKCGCGGN
HHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCC
LIIISSPRGGKFVGCSNYPDCKKTYSLPAGANVLKTTCEKCGLPLISYGKPRQRACLDFE
EEEEECCCCCCEEECCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCEEEEE
CANGGQKSTNDVVGECPDCGKDLIKRMGRFGEFIGCTGFPKCRFTSSIDDFEKSKKESEK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
KD
CC
>Mature Secondary Structure 
SELIICEKPKVAEKVAKALSDSPVKNSYKRVPYYEFTNGNGTKITVLSAVGHLFSLKAK
CCCEECCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHHHCC
NKKDKRLFDVEWVPLSETDKSKKYVKNYIDTIKKFSKDADRFIHACDYDTEGTLIGFNAL
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
RYICGEDSIDKSFRMKFSALTKKDLIESYSNAYPLKEDKSWVDSGETRHVLDFLFGVNIS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHCCCCC
KSMTDSVLNVTNRYVQLSAGRVQTPTLAILTEREKEIQKFIPEPYWLIKAKLQKSIVADH
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
KKGKIFDKKEVDAILKNCKGKDATVEKITNRKTKKDLPVPFELGTLQSEAYAQFGFTPRK
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHCCCCHHH
TQQIAQNLYVEGYTSYPRTSSQKLPESLGLPNILSQLSKHPKYKDKISQLEQPYKPHEGK
HHHHHHHHHHCCCCCCCCCCHHHCHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
KTDEAHPAIHPTGTLPKDISEDYQKIYDLITYRFISIFGKPAEMESIKVELDIGGEPFSF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHH
SRQRISKEGWLSLDPYQYKKVKNEEFPEIKEGQTTKAKVASEEKETKPPARYNQASIIRE
HHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
LEKRGLGTKSTRANIVSILYTRKYVEGKKIEVSQLGQQIINTLEKYSERITSEQMTREFE
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDISNIKENEITEATVIEDAKKELNGILDSIDDNIEDIGKELYGAYEQSRVVGKCGCGGN
HHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCC
LIIISSPRGGKFVGCSNYPDCKKTYSLPAGANVLKTTCEKCGLPLISYGKPRQRACLDFE
EEEEECCCCCCEEECCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCEEEEE
CANGGQKSTNDVVGECPDCGKDLIKRMGRFGEFIGCTGFPKCRFTSSIDDFEKSKKESEK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
KD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]