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The map label for this gene is fruK [H]

Identifier: 82750401

GI number: 82750401

Start: 712329

End: 713249

Strand: Direct

Name: fruK [H]

Synonym: SAB0648

Alternate gene names: 82750401

Gene position: 712329-713249 (Clockwise)

Preceding gene: 82750400

Following gene: 82750402

Centisome position: 25.97

GC content: 36.26

Gene sequence:

>921_bases
ATGATTTATACAGTGACTTTCAATCCTTCAATTGACTATGTCATTTTTACGAATGATTTTAAAATTGATGGTTTGAACAG
AGCAACAGCAACATATAAATTCGCTGGGGGAAAAGGTATTAATGTCTCACGTGTTTTAAAGACATTGGATGTTGAGTCAA
CTGCTTTGGGATTTGCAGGTGGATTTCCTGGGAAATTCATTATAAATACATTAAATAACAGTGCAATTCAATCGAATTTT
ATTGAAGTTGATGAAGATACACGTATTAATGTGAAATTAAAAACAGGACAAGAAACAGAAATCAATGCACCGGGTCCTCA
TATAACGTCAACACAATTTGAACAACTGTTACAACAAATTAAAAATACAACAAGCGATGATATAGTTATTGTTGCTGGAA
GTGTACCAAGTAGTATTCCAAGTGATGCGTATGCGCAAATTGCACAAATTACAGCACAGACAGGTGCTAAATTAGTAGTC
GACGCTGAAAAAGAATTGGCTGAAAGCGTTTTACCATATCATCCACTATTTATTAAACCTAATAAAGATGAATTAGAAGT
GATGTTTAATACAACAGTGAACTCAGACGCAGATGTTATTAAATATGGTCGTTTGCTAGTTGATAAAGGTGCGCAATCTG
TTATTGTCTCACTTGGCGGTGATGGTGCTATTTATATTGATAAAGAAATCAGTATTAAAGCAGTTAATCCACAAGGGAAA
GTGGTTAATACAGTTGGCTCTGGTGATAGTACAGTTGCAGGCATGGTGGCTGGAATTGCTTCAGGTTTAACGATTGAAAA
AGCATTCCAACAAGCAGTCGCATGCGGTACTGCCACGGCATTTGATGAGGACTTAGCAACACGGGACGCTATAGAAAAAA
TAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGA

Upstream 100 bases:

>100_bases
CTTTGCTAGAAAGTACGACAATCATCACATCTGAAAAAGCATTAAATCAAGAATCGTTAAAAGAATACCAACAAAAGTAT
CACTTTATAGGAGGGACTTT

Downstream 100 bases:

>100_bases
AAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGATAAAAATGGTGTTATT
GATGAGTTAGTAAATCAATT

Product: fructose 1-phosphate kinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAGGFPGKFIINTLNNSAIQSNF
IEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQIKNTTSDDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGK
VVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTISVLDGE

Sequences:

>Translated_306_residues
MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAGGFPGKFIINTLNNSAIQSNF
IEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQIKNTTSDDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGK
VVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTISVLDGE
>Mature_306_residues
MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAGGFPGKFIINTLNNSAIQSNF
IEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQIKNTTSDDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGK
VVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTISVLDGE

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=300, Percent_Identity=30.6666666666667, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788493, Length=266, Percent_Identity=32.3308270676692, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 32528; Mature: 32528

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAG
CEEEEEECCCCCEEEEECCEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHEEECCC
GFPGKFIINTLNNSAIQSNFIEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQI
CCCCCEEEEECCCCHHHCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KNTTSDDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVVDAEKELAESVLPYHPLFIKP
HCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCEEEEC
NKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGK
CCCCEEEEEECCCCCCHHHHHHHHHEECCCCCEEEEEECCCEEEEEECCEEEEEECCCCC
VVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTI
EEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
SVLDGE
EEECCC
>Mature Secondary Structure
MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAG
CEEEEEECCCCCEEEEECCEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHEEECCC
GFPGKFIINTLNNSAIQSNFIEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQI
CCCCCEEEEECCCCHHHCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KNTTSDDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVVDAEKELAESVLPYHPLFIKP
HCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCEEEEC
NKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGK
CCCCEEEEEECCCCCCHHHHHHHHHEECCCCCEEEEEECCCEEEEEECCEEEEEECCCCC
VVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTI
EEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
SVLDGE
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]