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The map label for this gene is tapD [H]

Identifier: 82703250

GI number: 82703250

Start: 2424846

End: 2425754

Strand: Reverse

Name: tapD [H]

Synonym: Nmul_A2131

Alternate gene names: 82703250

Gene position: 2425754-2424846 (Counterclockwise)

Preceding gene: 82703251

Following gene: 82703249

Centisome position: 76.18

GC content: 55.89

Gene sequence:

>909_bases
ATGTCTTTTATCTCCGTGCTGCAATACTCTCCGGTGTTCTTTGCCTCGTTCTGCGCGCTCATTGGTCTGGTCGCCGGCAG
TTTTTTGAATGTGGTTATCTACCGCCTGCCGAGAATGCTGGAGCGGGAATGGCGACAACAGTGCGCCGAACTGCAGGCAG
AACTGTCTTCCGGAACAAACGGGATAGCGCCAGCACACGAACCGCATGAAGCCCTTGCAGCGGAGCCGGCTTTCAATCTC
ATTACGCCGTCTTCCACTTGTCCCCATTGCGGGCACAGGATTACGGCGCTCGAAAATATTCCGCTCATCAGCTACATCGC
ATTGAGGGGACGCTGCTCGCAGTGCCGCACCGCGATTTCCATGCGCTATCCCGTTGTGGAAGGGTTAACCGCAGCGCTGA
GCGGCCTTGTCGCGTGGCATTTTGGTTATGGGGTTATCGCTTTTGCGGCGCTTGCGCTTGTATGGGCCATGGTTGCGCTT
GCTTTCATCGACCTGGATACTCAGTTGCTGCCCAATGACATTACCATCCCCCTGTTATGGGGAGGGTTATTGATTAACCT
GAGCGGTGGTTTTGCGGATATCCATTCTGCCGTGATTGGCGCCGTAGTGGGATATCTTGCCCTGTGGTCGGTGTATTGGG
GTTATAAACTCCTGACCGGCCGGGAGGGAATGGGTTATGGGGACTTCAAGTTGCTGGCCGCCATTGGCGCCTGGCTGGGG
TGGCAAATGCTGCCCCTGGTGATTCTGTCTTCATCCCTCGTGGGAAGCATGGCAGGGCTTGGCCTGATGCTGGCAGCAAA
GCACGGGCGTCATGTCCCTATTCCGTTCGGGCCTTATCTGGTGTGTGGGGGAATCGTGGCTCTGTTTTGGGGAAACGAGA
TCAATAGAGCTTACCTGGGGTCATTTTAG

Upstream 100 bases:

>100_bases
GTGTGGTAAAAGTGGTGCGGATCTTTCCTCCGTTAGCGCCAATCGAGGAGAAACTCCCAGACTCCTATGCGGAATCCTGA
TCCCCGGGGATGTCTTTTAA

Downstream 100 bases:

>100_bases
CCCCATGCTGGTAATCGGCCTGACTGGGGGCATCGGCAGCGGTAAAACCAGCGCCGCCAATATTTTTTCCGCGCTCGGCG
CCGGCGTGGTCGATACCGAT

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSFISVLQYSPVFFASFCALIGLVAGSFLNVVIYRLPRMLEREWRQQCAELQAELSSGTNGIAPAHEPHEALAAEPAFNL
ITPSSTCPHCGHRITALENIPLISYIALRGRCSQCRTAISMRYPVVEGLTAALSGLVAWHFGYGVIAFAALALVWAMVAL
AFIDLDTQLLPNDITIPLLWGGLLINLSGGFADIHSAVIGAVVGYLALWSVYWGYKLLTGREGMGYGDFKLLAAIGAWLG
WQMLPLVILSSSLVGSMAGLGLMLAAKHGRHVPIPFGPYLVCGGIVALFWGNEINRAYLGSF

Sequences:

>Translated_302_residues
MSFISVLQYSPVFFASFCALIGLVAGSFLNVVIYRLPRMLEREWRQQCAELQAELSSGTNGIAPAHEPHEALAAEPAFNL
ITPSSTCPHCGHRITALENIPLISYIALRGRCSQCRTAISMRYPVVEGLTAALSGLVAWHFGYGVIAFAALALVWAMVAL
AFIDLDTQLLPNDITIPLLWGGLLINLSGGFADIHSAVIGAVVGYLALWSVYWGYKLLTGREGMGYGDFKLLAAIGAWLG
WQMLPLVILSSSLVGSMAGLGLMLAAKHGRHVPIPFGPYLVCGGIVALFWGNEINRAYLGSF
>Mature_301_residues
SFISVLQYSPVFFASFCALIGLVAGSFLNVVIYRLPRMLEREWRQQCAELQAELSSGTNGIAPAHEPHEALAAEPAFNLI
TPSSTCPHCGHRITALENIPLISYIALRGRCSQCRTAISMRYPVVEGLTAALSGLVAWHFGYGVIAFAALALVWAMVALA
FIDLDTQLLPNDITIPLLWGGLLINLSGGFADIHSAVIGAVVGYLALWSVYWGYKLLTGREGMGYGDFKLLAAIGAWLGW
QMLPLVILSSSLVGSMAGLGLMLAAKHGRHVPIPFGPYLVCGGIVALFWGNEINRAYLGSF

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=286, Percent_Identity=36.7132867132867, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789732, Length=135, Percent_Identity=48.1481481481481, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32514; Mature: 32382

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFISVLQYSPVFFASFCALIGLVAGSFLNVVIYRLPRMLEREWRQQCAELQAELSSGTN
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIAPAHEPHEALAAEPAFNLITPSSTCPHCGHRITALENIPLISYIALRGRCSQCRTAIS
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCHHHHHHHHH
MRYPVVEGLTAALSGLVAWHFGYGVIAFAALALVWAMVALAFIDLDTQLLPNDITIPLLW
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHH
GGLLINLSGGFADIHSAVIGAVVGYLALWSVYWGYKLLTGREGMGYGDFKLLAAIGAWLG
CCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
WQMLPLVILSSSLVGSMAGLGLMLAAKHGRHVPIPFGPYLVCGGIVALFWGNEINRAYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
SF
CC
>Mature Secondary Structure 
SFISVLQYSPVFFASFCALIGLVAGSFLNVVIYRLPRMLEREWRQQCAELQAELSSGTN
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIAPAHEPHEALAAEPAFNLITPSSTCPHCGHRITALENIPLISYIALRGRCSQCRTAIS
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCHHHHHHHHH
MRYPVVEGLTAALSGLVAWHFGYGVIAFAALALVWAMVALAFIDLDTQLLPNDITIPLLW
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHH
GGLLINLSGGFADIHSAVIGAVVGYLALWSVYWGYKLLTGREGMGYGDFKLLAAIGAWLG
CCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
WQMLPLVILSSSLVGSMAGLGLMLAAKHGRHVPIPFGPYLVCGGIVALFWGNEINRAYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCC
SF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA