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The map label for this gene is ispD

Identifier: 82703246

GI number: 82703246

Start: 2421068

End: 2421766

Strand: Reverse

Name: ispD

Synonym: Nmul_A2127

Alternate gene names: 82703246

Gene position: 2421766-2421068 (Counterclockwise)

Preceding gene: 82703248

Following gene: 82703245

Centisome position: 76.05

GC content: 57.22

Gene sequence:

>699_bases
ATGCAAAATTTCTTCGCACTCATTCCGGCGGCAGGTTCAGGCTCGCGCATGGGCGACCGGATGCCCAAGCAATACTTGAC
CCTCGCAGGAAAACCCATGATTCATCATGCGCTGGCAACGTTATGCAACTCGCCGCGGCTTTCCCGAGTCTTTGTTGTGC
TGTCCCCGGGAGATGTAGAGTGGGCGCGGCATGACTGGTCAGAATTCTCCAGCAAGCTATCGATGCTTGAATGCGGCGGA
GCTACCCGCGCCGAGACCGTGCTGAATGGTTTAAAAGCAGCGCAGGAAGGGACGGCGATCGAAGATGATGACTGGGTGCT
GGTCCATGATGCTGCACGGCCCTGCTTGGGAGGGAAGCTTCTGGATAAGCTGATGGATGAACTGGAAGAGGACGAAGTGG
GCGGATTGCTCGCGGTTCCGGTTGCGGACACCTTGAAGCGAAGCGATCCCACTTGCCGGGCTGAACGTACAGAACCGAGA
GAAGGTCTTTGGCAGGCCCAGACACCGCAGATGTTCCGGTACCGAACCCTGGTGAACGCCCTGAGCGGGGCTGGAGGTGT
AACCATGACGGACGATGCAGGCGCTATCGAAGCACTCGGTCTGCGCCCGAAACTGGTTGTGAGCGATGCGCGCAACCTGA
AAGTTACTTATCCTCAGGATCTCGCGTTGGCGGAGTTGATACTGAAAAACTGTAAGTAG

Upstream 100 bases:

>100_bases
TGTTTTTACCGTATTTCCATTGCCCATTTTCGCGGTCTAACACATATTCACGTGCCGGTCCTCGTTTGACGCACCAATTC
AGCTACTTCCTGTAATATCC

Downstream 100 bases:

>100_bases
TGTAATGAGGGTGGGCGGCTTATTAGCCCGCTGAAAGCAGTTGTAGATCCAGGCGTCAGGTGAAAGATGTCGGGCAAAGC
CCGAGAGTCGCGCAAAGCAC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MQNFFALIPAAGSGSRMGDRMPKQYLTLAGKPMIHHALATLCNSPRLSRVFVVLSPGDVEWARHDWSEFSSKLSMLECGG
ATRAETVLNGLKAAQEGTAIEDDDWVLVHDAARPCLGGKLLDKLMDELEEDEVGGLLAVPVADTLKRSDPTCRAERTEPR
EGLWQAQTPQMFRYRTLVNALSGAGGVTMTDDAGAIEALGLRPKLVVSDARNLKVTYPQDLALAELILKNCK

Sequences:

>Translated_232_residues
MQNFFALIPAAGSGSRMGDRMPKQYLTLAGKPMIHHALATLCNSPRLSRVFVVLSPGDVEWARHDWSEFSSKLSMLECGG
ATRAETVLNGLKAAQEGTAIEDDDWVLVHDAARPCLGGKLLDKLMDELEEDEVGGLLAVPVADTLKRSDPTCRAERTEPR
EGLWQAQTPQMFRYRTLVNALSGAGGVTMTDDAGAIEALGLRPKLVVSDARNLKVTYPQDLALAELILKNCK
>Mature_232_residues
MQNFFALIPAAGSGSRMGDRMPKQYLTLAGKPMIHHALATLCNSPRLSRVFVVLSPGDVEWARHDWSEFSSKLSMLECGG
ATRAETVLNGLKAAQEGTAIEDDDWVLVHDAARPCLGGKLLDKLMDELEEDEVGGLLAVPVADTLKRSDPTCRAERTEPR
EGLWQAQTPQMFRYRTLVNALSGAGGVTMTDDAGAIEALGLRPKLVVSDARNLKVTYPQDLALAELILKNCK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=242, Percent_Identity=26.8595041322314, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=44.2477876106195, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_NITMU (Q2Y751)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412812.1
- ProteinModelPortal:   Q2Y751
- SMR:   Q2Y751
- STRING:   Q2Y751
- GeneID:   3786665
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2127
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   Q2Y751
- ProtClustDB:   PRK00155
- BioCyc:   NMUL323848:NMUL_A2127-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25232; Mature: 25232

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFFALIPAAGSGSRMGDRMPKQYLTLAGKPMIHHALATLCNSPRLSRVFVVLSPGDVE
CCCEEEEEECCCCCCCHHHHCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCC
WARHDWSEFSSKLSMLECGGATRAETVLNGLKAAQEGTAIEDDDWVLVHDAARPCLGGKL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH
LDKLMDELEEDEVGGLLAVPVADTLKRSDPTCRAERTEPREGLWQAQTPQMFRYRTLVNA
HHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHH
LSGAGGVTMTDDAGAIEALGLRPKLVVSDARNLKVTYPQDLALAELILKNCK
HCCCCCEEEECCCCCHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQNFFALIPAAGSGSRMGDRMPKQYLTLAGKPMIHHALATLCNSPRLSRVFVVLSPGDVE
CCCEEEEEECCCCCCCHHHHCHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCC
WARHDWSEFSSKLSMLECGGATRAETVLNGLKAAQEGTAIEDDDWVLVHDAARPCLGGKL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH
LDKLMDELEEDEVGGLLAVPVADTLKRSDPTCRAERTEPREGLWQAQTPQMFRYRTLVNA
HHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHH
LSGAGGVTMTDDAGAIEALGLRPKLVVSDARNLKVTYPQDLALAELILKNCK
HCCCCCEEEECCCCCHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA