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The map label for this gene is clpB [H]

Identifier: 82702670

GI number: 82702670

Start: 1760428

End: 1763037

Strand: Reverse

Name: clpB [H]

Synonym: Nmul_A1541

Alternate gene names: 82702670

Gene position: 1763037-1760428 (Counterclockwise)

Preceding gene: 82702671

Following gene: 82702669

Centisome position: 55.37

GC content: 56.21

Gene sequence:

>2610_bases
ATGCGTTTCGACAAATTAACTACAAAATTCCAGCAGGCGTTGTCCGATGCGCAGAGCATTGCTGTGGGTCAGGACAACCC
TTATATTGAACCGCAGCATTTACTGCTCGCACTTTTGCAACAGCAAGACGCAGGCACGGTTTCGTTGTTGCAGCGCGCAG
GGGCGAATGTGCCCCCCCTGCGCGAGGCACTGAAAAAATCGATTGAACGCCTTCCTAAAGTAGAGGGTACGGGTGGAGAG
ATCAACGTTTCGCGTGATCTGGGTAACCTGCTCAATCTGGCCGACAAGGAGGCGCAAAAACGGGGTGATCAATTCATTGC
TTCGGAGATGTTCCTGGTGGCGGCGCTTCAGGACAAAGGGGAGACCGGCAACCTGCTAAGGCAGAATGGGGCCAGTCGCA
ATGCGCTCGAACAAGCGATCAACGAGGTGCGCGGTGGCGAGAATGTAAGCAATCCTGAGGCCGAAGCCAGTCGGGAAGCA
TTGAAGAAATACACGCTCGATCTGACGGAACGGGCGCGCGCGGGAAAGCTTGATCCAGTTATCGGGCGCGATGATGAAAT
TCGGCGTACTGTTCAGGTATTGCAACGCCGTACCAAGAACAATCCCGTGCTGATCGGCGAACCGGGTGTGGGCAAGACTG
CTATTGTCGAGGGTTTGGCTCAGCGCATCGTCAATGGCGAAGTGCCGGAAACCCTCAAGGACAAGAAAGTACTTTCGCTG
GATATGGCCGCGCTGCTCGCGGGAGCCAAATACCGGGGTGAATTCGAGGAGCGCCTCAAGTCTGTCCTAAAGGAGATCGC
ACAGGCCGGGGGCAGCGTTATTGTCTTCATAGACGAGCTTCATACCATGGTAGGGGCCGGCAAGGCCGAGGGCGCGATCG
ATGCGGGCAACATGCTCAAACCCGCGCTGGCGCGCGGCGAATTGCATTGCGTGGGTGCCACCACCCTGGACGAATACCGC
AAATACATTGAGAAAGACGCCGCGCTGGAGCGGCGTTTCCAGAAAGTGTTCGTGGAAGAGCCGACGGTCGAGGCGACCAT
CGCCATCCTGCGCGGCTTGCAGGAACGATACGAGCTGCATCATGGCGTGGATATAACCGACCCCGCCATTGTTGCAGCAG
CCGAACTGTCGCATCGTTATATTACCGACCGCTTTCTGCCCGATAAGGCCATTGATCTGATCGATGAAGCCGCAGCCCGC
ATCCGGATGGAAATCGATTCCAAGCCGGAGGCAATGGACAGGCTCGACCGTCGCCTGATACAACTCAAGATCGAGCGCGA
GGCGGTGAAGAAGGAAAAGGATGAGGCGTCCCAGAAACGTCTTGCATTACTGGAAGACGAGATAGCCAAACTCGAGCGCG
AATACGCCGACCTGGAAGAAATCTGGAAAGCCGAAAAATCCCAGGTGCAGGGTACGCAGCATATCAAGGAAGAGATGGAA
AAGGTCAAGCTCGAAATCGAGGCGGCGACGCGCAAAGGAGATTGGCAGAAAGTTTCGGAGTTGCAATATGGGCGCCTTCC
GCAACTGGAGGCGCAACTTCAGATGCAGGATCGGCAGGCTGAGAAGGAGACCGTGGAGCAAAACAAGCCGAAGCTGCTTC
GAACCCAGGTTGGGGCGGAAGAAATCGCAGAAGTGGTTTCGCGCGCTACCGGCATCCCTGTTTCCAAGATGATGCAGGGT
GAACGAGAGAAACTGCTGCACATGGAGGAGAAGCTGCACGAGCGCGTAGTGGGGCAGGATGAAGCGGTACGGCTGGTGTC
GGATGCCATTCGCCGGTCGCGCGCAGGTCTGGCTGATCCGAACCGCCCTTATGGCTCTTTTCTGTTCCTGGGGCCGACAG
GGGTGGGTAAGACCGAGTTATGCAGGGCGCTGGCAGGATTTCTGTTCGATTCGGAAGAGCACATGATCCGCATCGACATG
TCGGAGTTCATGGAAAAACATTCGGTTGCGCGGCTTATTGGCGCTCCGCCGGGGTATGTGGGCTACGAGGAAGGCGGCTA
TCTGACGGAAGCCGTACGACGAAAACCCTACGCGGTTATTCTGCTGGATGAAGTGGAGAAGGCGCATCCCGACGTATTCA
ACGTCTTGTTGCAGGTGCTGGATGACGGCCGCATGACAGACGGGCAGGGACGCACGGTGGACTTCAAGAACACCGTTATC
GTCATGACTTCAAACCTCGGTTCGCAAATGATCCAGCAGATGGCAGGAGATGATTACCAGGTCATCAAGCTGGCGGTCAT
GGGCGAGGTGAAGGCATATTTCCGCCCGGAATTCATTAACCGGATTGACGAGGTGGTGGTGTTCCATGCGCTGGATGAGA
AGCATATCAAATCCATCGCGCGGATACAGCTGCAGCAACTGGAAAGGCGCCTGGCAAAACTTGAGATGCATCTTAAGGTG
ACGGATGCTGCACTTGCAGAAGTGGCCCAGGCGGGATTCGATCCAGTGTTCGGTGCCCGGCCTCTCAAGCGCGCGATACA
GGGCCAGATCGAGAACCCCTTGGCAAAGGAAATTCTCGAAGGTCACTTCGCTGCGAAGGATACCATCACAGTCGATGCGA
AAAATGGCGTGATCACTTTCTCGAAGGCAAGGGAATCCGTACCGGCTTGA

Upstream 100 bases:

>100_bases
TCCATCTTCCCGGAGGATTTGGACGCGTGAGGGGATATCTTGAAAATTCTACCGTATCCCATATATATAAGAATCATTCA
CTACCCATAAAGGGCTTCTC

Downstream 100 bases:

>100_bases
GGCGATTTCCGGCAACCAAAGACTTTCTTCCCGATCCTCTCCCGCCTGTTTAGCCAGTGGGAGAGGGAGAGGGAAGGGAG
CAGCTTTACAGTACCATCGT

Product: ATPase with chaperone activity

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 869; Mature: 869

Protein sequence:

>869_residues
MRFDKLTTKFQQALSDAQSIAVGQDNPYIEPQHLLLALLQQQDAGTVSLLQRAGANVPPLREALKKSIERLPKVEGTGGE
INVSRDLGNLLNLADKEAQKRGDQFIASEMFLVAALQDKGETGNLLRQNGASRNALEQAINEVRGGENVSNPEAEASREA
LKKYTLDLTERARAGKLDPVIGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKDKKVLSL
DMAALLAGAKYRGEFEERLKSVLKEIAQAGGSVIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYR
KYIEKDAALERRFQKVFVEEPTVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IRMEIDSKPEAMDRLDRRLIQLKIEREAVKKEKDEASQKRLALLEDEIAKLEREYADLEEIWKAEKSQVQGTQHIKEEME
KVKLEIEAATRKGDWQKVSELQYGRLPQLEAQLQMQDRQAEKETVEQNKPKLLRTQVGAEEIAEVVSRATGIPVSKMMQG
EREKLLHMEEKLHERVVGQDEAVRLVSDAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCRALAGFLFDSEEHMIRIDM
SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYAVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQMIQQMAGDDYQVIKLAVMGEVKAYFRPEFINRIDEVVVFHALDEKHIKSIARIQLQQLERRLAKLEMHLKV
TDAALAEVAQAGFDPVFGARPLKRAIQGQIENPLAKEILEGHFAAKDTITVDAKNGVITFSKARESVPA

Sequences:

>Translated_869_residues
MRFDKLTTKFQQALSDAQSIAVGQDNPYIEPQHLLLALLQQQDAGTVSLLQRAGANVPPLREALKKSIERLPKVEGTGGE
INVSRDLGNLLNLADKEAQKRGDQFIASEMFLVAALQDKGETGNLLRQNGASRNALEQAINEVRGGENVSNPEAEASREA
LKKYTLDLTERARAGKLDPVIGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKDKKVLSL
DMAALLAGAKYRGEFEERLKSVLKEIAQAGGSVIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYR
KYIEKDAALERRFQKVFVEEPTVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IRMEIDSKPEAMDRLDRRLIQLKIEREAVKKEKDEASQKRLALLEDEIAKLEREYADLEEIWKAEKSQVQGTQHIKEEME
KVKLEIEAATRKGDWQKVSELQYGRLPQLEAQLQMQDRQAEKETVEQNKPKLLRTQVGAEEIAEVVSRATGIPVSKMMQG
EREKLLHMEEKLHERVVGQDEAVRLVSDAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCRALAGFLFDSEEHMIRIDM
SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYAVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQMIQQMAGDDYQVIKLAVMGEVKAYFRPEFINRIDEVVVFHALDEKHIKSIARIQLQQLERRLAKLEMHLKV
TDAALAEVAQAGFDPVFGARPLKRAIQGQIENPLAKEILEGHFAAKDTITVDAKNGVITFSKARESVPA
>Mature_869_residues
MRFDKLTTKFQQALSDAQSIAVGQDNPYIEPQHLLLALLQQQDAGTVSLLQRAGANVPPLREALKKSIERLPKVEGTGGE
INVSRDLGNLLNLADKEAQKRGDQFIASEMFLVAALQDKGETGNLLRQNGASRNALEQAINEVRGGENVSNPEAEASREA
LKKYTLDLTERARAGKLDPVIGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKDKKVLSL
DMAALLAGAKYRGEFEERLKSVLKEIAQAGGSVIVFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYR
KYIEKDAALERRFQKVFVEEPTVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAR
IRMEIDSKPEAMDRLDRRLIQLKIEREAVKKEKDEASQKRLALLEDEIAKLEREYADLEEIWKAEKSQVQGTQHIKEEME
KVKLEIEAATRKGDWQKVSELQYGRLPQLEAQLQMQDRQAEKETVEQNKPKLLRTQVGAEEIAEVVSRATGIPVSKMMQG
EREKLLHMEEKLHERVVGQDEAVRLVSDAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCRALAGFLFDSEEHMIRIDM
SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYAVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQMIQQMAGDDYQVIKLAVMGEVKAYFRPEFINRIDEVVVFHALDEKHIKSIARIQLQQLERRLAKLEMHLKV
TDAALAEVAQAGFDPVFGARPLKRAIQGQIENPLAKEILEGHFAAKDTITVDAKNGVITFSKARESVPA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=316, Percent_Identity=36.3924050632911, Blast_Score=204, Evalue=4e-52,
Organism=Escherichia coli, GI1788943, Length=853, Percent_Identity=68.3470105509965, Blast_Score=1184, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=396, Percent_Identity=42.1717171717172, Blast_Score=286, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=710, Percent_Identity=52.8169014084507, Blast_Score=761, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=867, Percent_Identity=43.1372549019608, Blast_Score=698, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96862; Mature: 96862

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDKLTTKFQQALSDAQSIAVGQDNPYIEPQHLLLALLQQQDAGTVSLLQRAGANVPPL
CCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCHH
REALKKSIERLPKVEGTGGEINVSRDLGNLLNLADKEAQKRGDQFIASEMFLVAALQDKG
HHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETGNLLRQNGASRNALEQAINEVRGGENVSNPEAEASREALKKYTLDLTERARAGKLDPV
CCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKDKKVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCHHHHH
DMAALLAGAKYRGEFEERLKSVLKEIAQAGGSVIVFIDELHTMVGAGKAEGAIDAGNMLK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRKYIEKDAALERRFQKVFVEEPTVEATIAILRGLQERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIRMEIDSKPEAMDRLDRRLI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHH
QLKIEREAVKKEKDEASQKRLALLEDEIAKLEREYADLEEIWKAEKSQVQGTQHIKEEME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KVKLEIEAATRKGDWQKVSELQYGRLPQLEAQLQMQDRQAEKETVEQNKPKLLRTQVGAE
HHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHH
EIAEVVSRATGIPVSKMMQGEREKLLHMEEKLHERVVGQDEAVRLVSDAIRRSRAGLADP
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NRPYGSFLFLGPTGVGKTELCRALAGFLFDSEEHMIRIDMSEFMEKHSVARLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGYLTEAVRRKPYAVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE
VMTSNLGSQMIQQMAGDDYQVIKLAVMGEVKAYFRPEFINRIDEVVVFHALDEKHIKSIA
EEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHH
RIQLQQLERRLAKLEMHLKVTDAALAEVAQAGFDPVFGARPLKRAIQGQIENPLAKEILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
GHFAAKDTITVDAKNGVITFSKARESVPA
HCCCCCCCEEEECCCCEEEEHHHHCCCCC
>Mature Secondary Structure
MRFDKLTTKFQQALSDAQSIAVGQDNPYIEPQHLLLALLQQQDAGTVSLLQRAGANVPPL
CCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCHH
REALKKSIERLPKVEGTGGEINVSRDLGNLLNLADKEAQKRGDQFIASEMFLVAALQDKG
HHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETGNLLRQNGASRNALEQAINEVRGGENVSNPEAEASREALKKYTLDLTERARAGKLDPV
CCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKDKKVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCHHHHH
DMAALLAGAKYRGEFEERLKSVLKEIAQAGGSVIVFIDELHTMVGAGKAEGAIDAGNMLK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLDEYRKYIEKDAALERRFQKVFVEEPTVEATIAILRGLQERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAARIRMEIDSKPEAMDRLDRRLI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHH
QLKIEREAVKKEKDEASQKRLALLEDEIAKLEREYADLEEIWKAEKSQVQGTQHIKEEME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KVKLEIEAATRKGDWQKVSELQYGRLPQLEAQLQMQDRQAEKETVEQNKPKLLRTQVGAE
HHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHH
EIAEVVSRATGIPVSKMMQGEREKLLHMEEKLHERVVGQDEAVRLVSDAIRRSRAGLADP
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NRPYGSFLFLGPTGVGKTELCRALAGFLFDSEEHMIRIDMSEFMEKHSVARLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGYLTEAVRRKPYAVILLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE
VMTSNLGSQMIQQMAGDDYQVIKLAVMGEVKAYFRPEFINRIDEVVVFHALDEKHIKSIA
EEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHH
RIQLQQLERRLAKLEMHLKVTDAALAEVAQAGFDPVFGARPLKRAIQGQIENPLAKEILE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
GHFAAKDTITVDAKNGVITFSKARESVPA
HCCCCCCCEEEECCCCEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]