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The map label for this gene is yerD [H]

Identifier: 82702671

GI number: 82702671

Start: 1763177

End: 1764658

Strand: Reverse

Name: yerD [H]

Synonym: Nmul_A1542

Alternate gene names: 82702671

Gene position: 1764658-1763177 (Counterclockwise)

Preceding gene: 82702673

Following gene: 82702670

Centisome position: 55.42

GC content: 53.98

Gene sequence:

>1482_bases
ATGTCTGATGCATTGAAAGTAATCTCATACGGTTTCGGCACGATTGCCGGTCTGATCGTGATAGGCATAGTCATATTCTG
GTTCATTCAGGATGTAACACAGAAGAAGCATTCCATCCTGCGTAACTATCCAGTAATCGGACGCCTGCGATTTTTCCTGG
AGAAACAGGGAGAGTATTTCCGGCAATACTTTTTTTCCAGTGATCGGGGCGAAATGCCCTTTAATCGCGCTACCCGCTCA
TGGGTGTACAGGAATGCAAAGAATGAGGGGGGTATCGTCGGTTTTGGTTCGACCAATGATTTGCGGGAGCCAGGCTCTAT
TTTTTTCGTCAATGTCGCTTTCCCCGTATTGGAGAGCGACCGGTTGCCCACTCCGCCCTTGGTGATTGGCGAGGACTATT
GCGAGCACCCTTTCAATGCGCAATCCATTATCAATATCAGTGGAATGAGTTACGGCGCGATCTCAGCCCCCGCGGTCCGC
GCGTTGTCGCTGGGAGCGGCGGAGGCGGGATGCTGGCTGAACACAGGGGAAGGTGGGCTCTCACCTTATCATCTGGAAGG
GGGTGGCGATATTGTCATGCAAATCGGTACTGCAAAGTATGGTATTCGCGACCAGGAAGGTAATTTTTCACCGGAGCGGG
CAAAGGAACTTGCGAAGGTGGTCAAGGCATTTGAAATAAAACTCTCTCAGGGAGCGAAGCCGGGTAAGGGAGGATTGCTT
CCTGCAACCAAGGTAACCAATGAAATTTCGGCTATCCGCGGTATTCCTGCTCATCGTGATTCAATCAGCCCCGATCGTCA
TCGCGACATCGGGAATGTGGATGAACTGCTCGACAAGATTGCTCATATCCGCGATCTCACCGGGCGGCCCGTGGGTGTCA
AGGCTGCCATCGGGGGATGGCACTTTATTAACGAACTGTGCGACGCAGTAAATCGCCGGGGTTCCGAATATGCGCCGGAT
TTTCTTACTATTGATGGCGGCGAAGGGGGCAGCGGGGCCGCCCCCCAGACGCTGTTCGATCATATGAGCCTGCCCATTGC
GGAAGCATTACCACGAGTGGTGGATGCGTTACTGGAATCCGGCTTGAGAGACCGCATACGTGTGGTTGCGGCAGGTAAGC
TGGTGACAGCGGCACGGGCGGCGTGGGCGTTGTGTGCGGGCGCGGATTTTGTCAACACCGCGCGCGGGTTCATGTTTTCA
CTCGGCTGTATCCAGGCCATGCGATGTCACACGAATACCTGCCCTACTGGTATCACCACCCATAATCGGCGTTTGCAGCG
GGGTCTGGTGGTGCAGGAGAAATACCTGCGTGTTGCCAATTATGCCAGAAACATGAACAGGGAAATCGATATGATTGCCT
ACTCCTGCGGCTTGCGCCACGCCCGCCAATTGAAGCGCGAGCATGTGCGCATCGTGGAAACGGCGGGCAAAAGCGTGGCT
CTCAACATTTTGTACCCTTATCCGGAAATACCTTCGCTTTGA

Upstream 100 bases:

>100_bases
TTGACTTCGGTTTCCAGATACGTGAAATGATCGTAAAACATTTTTATTATGTTCACCGGAACGAATGGAGTATTTTAGCT
GAATGTGATACGGAGGCATC

Downstream 100 bases:

>100_bases
CATCGCATGCGATACAGCTATGGCTTGCTTATTGCATCCTCCATCTTCCCGGAGGATTTGGACGCGTGAGGGGATATCTT
GAAAATTCTACCGTATCCCA

Product: ferredoxin-dependent glutamate synthase

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSDALKVISYGFGTIAGLIVIGIVIFWFIQDVTQKKHSILRNYPVIGRLRFFLEKQGEYFRQYFFSSDRGEMPFNRATRS
WVYRNAKNEGGIVGFGSTNDLREPGSIFFVNVAFPVLESDRLPTPPLVIGEDYCEHPFNAQSIINISGMSYGAISAPAVR
ALSLGAAEAGCWLNTGEGGLSPYHLEGGGDIVMQIGTAKYGIRDQEGNFSPERAKELAKVVKAFEIKLSQGAKPGKGGLL
PATKVTNEISAIRGIPAHRDSISPDRHRDIGNVDELLDKIAHIRDLTGRPVGVKAAIGGWHFINELCDAVNRRGSEYAPD
FLTIDGGEGGSGAAPQTLFDHMSLPIAEALPRVVDALLESGLRDRIRVVAAGKLVTAARAAWALCAGADFVNTARGFMFS
LGCIQAMRCHTNTCPTGITTHNRRLQRGLVVQEKYLRVANYARNMNREIDMIAYSCGLRHARQLKREHVRIVETAGKSVA
LNILYPYPEIPSL

Sequences:

>Translated_493_residues
MSDALKVISYGFGTIAGLIVIGIVIFWFIQDVTQKKHSILRNYPVIGRLRFFLEKQGEYFRQYFFSSDRGEMPFNRATRS
WVYRNAKNEGGIVGFGSTNDLREPGSIFFVNVAFPVLESDRLPTPPLVIGEDYCEHPFNAQSIINISGMSYGAISAPAVR
ALSLGAAEAGCWLNTGEGGLSPYHLEGGGDIVMQIGTAKYGIRDQEGNFSPERAKELAKVVKAFEIKLSQGAKPGKGGLL
PATKVTNEISAIRGIPAHRDSISPDRHRDIGNVDELLDKIAHIRDLTGRPVGVKAAIGGWHFINELCDAVNRRGSEYAPD
FLTIDGGEGGSGAAPQTLFDHMSLPIAEALPRVVDALLESGLRDRIRVVAAGKLVTAARAAWALCAGADFVNTARGFMFS
LGCIQAMRCHTNTCPTGITTHNRRLQRGLVVQEKYLRVANYARNMNREIDMIAYSCGLRHARQLKREHVRIVETAGKSVA
LNILYPYPEIPSL
>Mature_492_residues
SDALKVISYGFGTIAGLIVIGIVIFWFIQDVTQKKHSILRNYPVIGRLRFFLEKQGEYFRQYFFSSDRGEMPFNRATRSW
VYRNAKNEGGIVGFGSTNDLREPGSIFFVNVAFPVLESDRLPTPPLVIGEDYCEHPFNAQSIINISGMSYGAISAPAVRA
LSLGAAEAGCWLNTGEGGLSPYHLEGGGDIVMQIGTAKYGIRDQEGNFSPERAKELAKVVKAFEIKLSQGAKPGKGGLLP
ATKVTNEISAIRGIPAHRDSISPDRHRDIGNVDELLDKIAHIRDLTGRPVGVKAAIGGWHFINELCDAVNRRGSEYAPDF
LTIDGGEGGSGAAPQTLFDHMSLPIAEALPRVVDALLESGLRDRIRVVAAGKLVTAARAAWALCAGADFVNTARGFMFSL
GCIQAMRCHTNTCPTGITTHNRRLQRGLVVQEKYLRVANYARNMNREIDMIAYSCGLRHARQLKREHVRIVETAGKSVAL
NILYPYPEIPSL

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=270, Percent_Identity=29.2592592592593, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17570289, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320030, Length=334, Percent_Identity=28.4431137724551, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI28574881, Length=322, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24665539, Length=322, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 53962; Mature: 53830

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALKVISYGFGTIAGLIVIGIVIFWFIQDVTQKKHSILRNYPVIGRLRFFLEKQGEYF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RQYFFSSDRGEMPFNRATRSWVYRNAKNEGGIVGFGSTNDLREPGSIFFVNVAFPVLESD
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEEEEEHHHCCC
RLPTPPLVIGEDYCEHPFNAQSIINISGMSYGAISAPAVRALSLGAAEAGCWLNTGEGGL
CCCCCCEEECCHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCC
SPYHLEGGGDIVMQIGTAKYGIRDQEGNFSPERAKELAKVVKAFEIKLSQGAKPGKGGLL
CCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PATKVTNEISAIRGIPAHRDSISPDRHRDIGNVDELLDKIAHIRDLTGRPVGVKAAIGGW
CHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHH
HFINELCDAVNRRGSEYAPDFLTIDGGEGGSGAAPQTLFDHMSLPIAEALPRVVDALLES
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
GLRDRIRVVAAGKLVTAARAAWALCAGADFVNTARGFMFSLGCIQAMRCHTNTCPTGITT
CHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HNRRLQRGLVVQEKYLRVANYARNMNREIDMIAYSCGLRHARQLKREHVRIVETAGKSVA
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LNILYPYPEIPSL
EEEEECCCCCCCC
>Mature Secondary Structure 
SDALKVISYGFGTIAGLIVIGIVIFWFIQDVTQKKHSILRNYPVIGRLRFFLEKQGEYF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RQYFFSSDRGEMPFNRATRSWVYRNAKNEGGIVGFGSTNDLREPGSIFFVNVAFPVLESD
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEEEEEHHHCCC
RLPTPPLVIGEDYCEHPFNAQSIINISGMSYGAISAPAVRALSLGAAEAGCWLNTGEGGL
CCCCCCEEECCHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCC
SPYHLEGGGDIVMQIGTAKYGIRDQEGNFSPERAKELAKVVKAFEIKLSQGAKPGKGGLL
CCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PATKVTNEISAIRGIPAHRDSISPDRHRDIGNVDELLDKIAHIRDLTGRPVGVKAAIGGW
CHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHH
HFINELCDAVNRRGSEYAPDFLTIDGGEGGSGAAPQTLFDHMSLPIAEALPRVVDALLES
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
GLRDRIRVVAAGKLVTAARAAWALCAGADFVNTARGFMFSLGCIQAMRCHTNTCPTGITT
CHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HNRRLQRGLVVQEKYLRVANYARNMNREIDMIAYSCGLRHARQLKREHVRIVETAGKSVA
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LNILYPYPEIPSL
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]