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The map label for this gene is dapA

Identifier: 82702669

GI number: 82702669

Start: 1759287

End: 1760189

Strand: Reverse

Name: dapA

Synonym: Nmul_A1540

Alternate gene names: 82702669

Gene position: 1760189-1759287 (Counterclockwise)

Preceding gene: 82702670

Following gene: 82702668

Centisome position: 55.28

GC content: 53.93

Gene sequence:

>903_bases
ATGGATAACGTCGCAATAAAAGGCAGTCTGGTCGCCATTGTCACCCCGATGCATGAAAATGGCGAACTGGATCTGGAGCG
CTTCCAATCCTTGATCGACTTTCATGTGACGGAAGGGACCGATGGTATCGTGGTGGTAGGAACCACCGGCGAATCACCGA
CCGTGGATTTCGAAGAGCACCATCTGTTGATCAAAACCGCAGTGGAGCAGGCGGCAGGGCGGGTACCGGTAATTGCGGGA
ACCGGAGCCAATTCCACGCGCGAGGCAATTGACCTTTCCATCTATGCGAAGAATGCGGGAGCGGATGCAAGCCTGTCGGT
TGTACCGTATTACAACAAGCCCACGCAGGAGGGTTTATACCAGCATTTCAGAGCGGTGGCGGAGGCTGTGGATATACCGC
AGATACTATACAACGTGCCCGGCAGGACGGTGGCAGATATTGCCAACGATACGGTCCTTCGTCTTGCGCAAATTCCCAAC
ATTGTCGGAATCAAGGATGCAACGGGTGATATCGGTCGCGGATTCGATCTGTTGTGCCGTGCTCCCGAAGATTTTGCAAT
CTATAGCGGCGATGATGCCAGTGCCCTGGCTTTGTTGCTGCTCGGCGGGCATGGCGTTATTTCCGTCACCGCCAACGTGG
CGCCGAAGCTCATGCATGAGATGTGCATTGCGGCATTTGCCGGTGACCTGGCTGCTGCCCGCGCTGCAAACAGAAAGCTT
TTGAGATTGCATCTGGATTTATTCATAGAGGCCAATCCTATTCCTGTGAAATGGGCGGTTGCGCAAATGGGATTGATAGG
CGAGGGGTTGCGGTTGCCACTCACACCGTTGTCGAATCGATATCATCAGACTCTCAGGGAAGCGATGAGCGAGGCGGGAA
TCGATTTGGCGATATCTGTTTAA

Upstream 100 bases:

>100_bases
GCTTCTCCCGGCGCTGTCTGACAACGCGACCTGTACCGTCGCGAATATACTTTGCGGGTGCCGGATTCAGGTAAAATAGG
AGTTTTTTTCGAGTCGCGTC

Downstream 100 bases:

>100_bases
ATGACTGCCCGAATTCGAGAGCCGGGAGCAGGATTTTCAGGAATCAAGTATTACACATGAGATCGCAGATAATGGCTGCG
CTGTGCCTGGCAGCGGCCGT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDNVAIKGSLVAIVTPMHENGELDLERFQSLIDFHVTEGTDGIVVVGTTGESPTVDFEEHHLLIKTAVEQAAGRVPVIAG
TGANSTREAIDLSIYAKNAGADASLSVVPYYNKPTQEGLYQHFRAVAEAVDIPQILYNVPGRTVADIANDTVLRLAQIPN
IVGIKDATGDIGRGFDLLCRAPEDFAIYSGDDASALALLLLGGHGVISVTANVAPKLMHEMCIAAFAGDLAAARAANRKL
LRLHLDLFIEANPIPVKWAVAQMGLIGEGLRLPLTPLSNRYHQTLREAMSEAGIDLAISV

Sequences:

>Translated_300_residues
MDNVAIKGSLVAIVTPMHENGELDLERFQSLIDFHVTEGTDGIVVVGTTGESPTVDFEEHHLLIKTAVEQAAGRVPVIAG
TGANSTREAIDLSIYAKNAGADASLSVVPYYNKPTQEGLYQHFRAVAEAVDIPQILYNVPGRTVADIANDTVLRLAQIPN
IVGIKDATGDIGRGFDLLCRAPEDFAIYSGDDASALALLLLGGHGVISVTANVAPKLMHEMCIAAFAGDLAAARAANRKL
LRLHLDLFIEANPIPVKWAVAQMGLIGEGLRLPLTPLSNRYHQTLREAMSEAGIDLAISV
>Mature_300_residues
MDNVAIKGSLVAIVTPMHENGELDLERFQSLIDFHVTEGTDGIVVVGTTGESPTVDFEEHHLLIKTAVEQAAGRVPVIAG
TGANSTREAIDLSIYAKNAGADASLSVVPYYNKPTQEGLYQHFRAVAEAVDIPQILYNVPGRTVADIANDTVLRLAQIPN
IVGIKDATGDIGRGFDLLCRAPEDFAIYSGDDASALALLLLGGHGVISVTANVAPKLMHEMCIAAFAGDLAAARAANRKL
LRLHLDLFIEANPIPVKWAVAQMGLIGEGLRLPLTPLSNRYHQTLREAMSEAGIDLAISV

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=296, Percent_Identity=28.0405405405405, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI13540533, Length=237, Percent_Identity=29.535864978903, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788823, Length=288, Percent_Identity=52.7777777777778, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082415, Length=273, Percent_Identity=34.7985347985348, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1786463, Length=240, Percent_Identity=33.75, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789620, Length=295, Percent_Identity=27.1186440677966, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_NITMU (Q2Y8S8)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412235.1
- HSSP:   Q9X1K9
- ProteinModelPortal:   Q2Y8S8
- SMR:   Q2Y8S8
- STRING:   Q2Y8S8
- GeneID:   3785613
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1540
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- PhylomeDB:   Q2Y8S8
- ProtClustDB:   PRK03170
- BioCyc:   NMUL323848:NMUL_A1540-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31934; Mature: 31934

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 165-165 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNVAIKGSLVAIVTPMHENGELDLERFQSLIDFHVTEGTDGIVVVGTTGESPTVDFEEH
CCCEEEECEEEEEEECCCCCCCCCHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCHHHC
HLLIKTAVEQAAGRVPVIAGTGANSTREAIDLSIYAKNAGADASLSVVPYYNKPTQEGLY
EEHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHH
QHFRAVAEAVDIPQILYNVPGRTVADIANDTVLRLAQIPNIVGIKDATGDIGRGFDLLCR
HHHHHHHHHHCHHHHHHCCCCCHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCCCCEEEE
APEDFAIYSGDDASALALLLLGGHGVISVTANVAPKLMHEMCIAAFAGDLAAARAANRKL
CCCCCEEECCCCHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCHHE
LRLHLDLFIEANPIPVKWAVAQMGLIGEGLRLPLTPLSNRYHQTLREAMSEAGIDLAISV
EEEEEEEEEECCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MDNVAIKGSLVAIVTPMHENGELDLERFQSLIDFHVTEGTDGIVVVGTTGESPTVDFEEH
CCCEEEECEEEEEEECCCCCCCCCHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCHHHC
HLLIKTAVEQAAGRVPVIAGTGANSTREAIDLSIYAKNAGADASLSVVPYYNKPTQEGLY
EEHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHH
QHFRAVAEAVDIPQILYNVPGRTVADIANDTVLRLAQIPNIVGIKDATGDIGRGFDLLCR
HHHHHHHHHHCHHHHHHCCCCCHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCCCCEEEE
APEDFAIYSGDDASALALLLLGGHGVISVTANVAPKLMHEMCIAAFAGDLAAARAANRKL
CCCCCEEECCCCHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCHHE
LRLHLDLFIEANPIPVKWAVAQMGLIGEGLRLPLTPLSNRYHQTLREAMSEAGIDLAISV
EEEEEEEEEECCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA