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The map label for this gene is fcl [H]

Identifier: 78779698

GI number: 78779698

Start: 1226341

End: 1227339

Strand: Reverse

Name: fcl [H]

Synonym: PMT9312_1315

Alternate gene names: 78779698

Gene position: 1227339-1226341 (Counterclockwise)

Preceding gene: 78779699

Following gene: 78779697

Centisome position: 71.81

GC content: 30.93

Gene sequence:

>999_bases
TTGCAAAAAAATCTTCTATTAAACTCAAAAGTATTTATTGCAGGCCATAAAGGAATGGTAGGTAGTGCAATATTTAGGAA
GTTAGATAATTTAGGTTACAAGAATATAATTACAGTCACAAAAAAAGACTTAAATCTAGAGGATGCAAATAAAGTAAGAC
AGTGGTTTGAAAACTACAAACCAGATATTGTGATAATCGCAGCCGCAAAAGTTGGTGGAATAAATGCAAATAATAAATAT
CCTGTTGACTTTTTACTAAAGAACTTAAAAATTCAAAATAACTTAATAGAGGCTTCTTGGTTAAATAATGTCAATAGATT
ACTTTTTCTTGGTAGTAGTTGTATTTATCCAAAATTTTCAACCCAACCAATTAAAGAAGAATATTTATTAACGGGAGAAT
TAGAACCCACAAATGAATGGTACGCGCTAGCAAAAATTGCAGGTATAAAATTATGTCAGGCGCTTAGGAAGCAATACGGA
TTTGATGCAATAAGTTTAATGCCTACAAATTTATATGGTCCAGGCGATAATTATAATTTATCTTCTTCTCATGTTTTGCC
TGCCTTAATAAGAAAATTTTATGAAGGGAAAGAAAATAATTTAAATGAAGTAACATGTTGGGGTACTGGTAACCCTAGAA
GAGAATTTTTACATGTTGATGACTTAGCCGAAGCGGTTGTTTTTGTTCTGAAAAATTGGCATCCTTCACTTCCAGAAGCC
CCAAAAGATTCTCGCAATCAAACACTCCCTTTTTTAAATGTTGGAACAGGGAATGATATTTCTATAAAAGAATTAGCTAA
TTTGATTGCAAAAGAAATAGGCTATTGTGGAAAAATTATCTGGGACCAATCAAAGCCAGATGGAATGTTTAGGAAGCAAC
TTGATGTTTCAAATATTATTAATTTGGGTTGGGAACCTAAGATTAAATTTAGTGATGGTATTAAAGATTCAATTATCGGT
TTTAAAAATGAACTTAAGACAAATACTCTAAGAATATGA

Upstream 100 bases:

>100_bases
ATTTGGAGGTATGCAAAGGCTTAGTAAATTAAAAAAAGTTACTTATGCATTAATACAAATGTTAACTTTGATTTAAAATA
AATTTATACAAGAAATTCTT

Downstream 100 bases:

>100_bases
GTTTTAAAAAAGTTTATTATTTAATGTTTTTTTTTAGTTTATTTGAGTTTAGTAAAATATGAAGGAAAATTATCTTGTTA
AAGATCAAATAAGTTTGTTT

Product: NAD dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQKNLLLNSKVFIAGHKGMVGSAIFRKLDNLGYKNIITVTKKDLNLEDANKVRQWFENYKPDIVIIAAAKVGGINANNKY
PVDFLLKNLKIQNNLIEASWLNNVNRLLFLGSSCIYPKFSTQPIKEEYLLTGELEPTNEWYALAKIAGIKLCQALRKQYG
FDAISLMPTNLYGPGDNYNLSSSHVLPALIRKFYEGKENNLNEVTCWGTGNPRREFLHVDDLAEAVVFVLKNWHPSLPEA
PKDSRNQTLPFLNVGTGNDISIKELANLIAKEIGYCGKIIWDQSKPDGMFRKQLDVSNIINLGWEPKIKFSDGIKDSIIG
FKNELKTNTLRI

Sequences:

>Translated_332_residues
MQKNLLLNSKVFIAGHKGMVGSAIFRKLDNLGYKNIITVTKKDLNLEDANKVRQWFENYKPDIVIIAAAKVGGINANNKY
PVDFLLKNLKIQNNLIEASWLNNVNRLLFLGSSCIYPKFSTQPIKEEYLLTGELEPTNEWYALAKIAGIKLCQALRKQYG
FDAISLMPTNLYGPGDNYNLSSSHVLPALIRKFYEGKENNLNEVTCWGTGNPRREFLHVDDLAEAVVFVLKNWHPSLPEA
PKDSRNQTLPFLNVGTGNDISIKELANLIAKEIGYCGKIIWDQSKPDGMFRKQLDVSNIINLGWEPKIKFSDGIKDSIIG
FKNELKTNTLRI
>Mature_332_residues
MQKNLLLNSKVFIAGHKGMVGSAIFRKLDNLGYKNIITVTKKDLNLEDANKVRQWFENYKPDIVIIAAAKVGGINANNKY
PVDFLLKNLKIQNNLIEASWLNNVNRLLFLGSSCIYPKFSTQPIKEEYLLTGELEPTNEWYALAKIAGIKLCQALRKQYG
FDAISLMPTNLYGPGDNYNLSSSHVLPALIRKFYEGKENNLNEVTCWGTGNPRREFLHVDDLAEAVVFVLKNWHPSLPEA
PKDSRNQTLPFLNVGTGNDISIKELANLIAKEIGYCGKIIWDQSKPDGMFRKQLDVSNIINLGWEPKIKFSDGIKDSIIG
FKNELKTNTLRI

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=306, Percent_Identity=32.6797385620915, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=22.8070175438596, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788365, Length=308, Percent_Identity=51.9480519480519, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17554428, Length=321, Percent_Identity=29.2834890965732, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=29.2604501607717, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 37505; Mature: 37505

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNLLLNSKVFIAGHKGMVGSAIFRKLDNLGYKNIITVTKKDLNLEDANKVRQWFENYK
CCCCEEECCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PDIVIIAAAKVGGINANNKYPVDFLLKNLKIQNNLIEASWLNNVNRLLFLGSSCIYPKFS
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEHHHHHCCCCEEEEECCCCCCCCCC
TQPIKEEYLLTGELEPTNEWYALAKIAGIKLCQALRKQYGFDAISLMPTNLYGPGDNYNL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
SSSHVLPALIRKFYEGKENNLNEVTCWGTGNPRREFLHVDDLAEAVVFVLKNWHPSLPEA
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHEEEHHHHHHHHHHHHHHCCCCCCCC
PKDSRNQTLPFLNVGTGNDISIKELANLIAKEIGYCGKIIWDQSKPDGMFRKQLDVSNII
CCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHCCHHHHH
NLGWEPKIKFSDGIKDSIIGFKNELKTNTLRI
HCCCCCCEEECCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQKNLLLNSKVFIAGHKGMVGSAIFRKLDNLGYKNIITVTKKDLNLEDANKVRQWFENYK
CCCCEEECCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PDIVIIAAAKVGGINANNKYPVDFLLKNLKIQNNLIEASWLNNVNRLLFLGSSCIYPKFS
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCEEHHHHHCCCCEEEEECCCCCCCCCC
TQPIKEEYLLTGELEPTNEWYALAKIAGIKLCQALRKQYGFDAISLMPTNLYGPGDNYNL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
SSSHVLPALIRKFYEGKENNLNEVTCWGTGNPRREFLHVDDLAEAVVFVLKNWHPSLPEA
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHEEEHHHHHHHHHHHHHHCCCCCCCC
PKDSRNQTLPFLNVGTGNDISIKELANLIAKEIGYCGKIIWDQSKPDGMFRKQLDVSNII
CCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHCCHHHHH
NLGWEPKIKFSDGIKDSIIGFKNELKTNTLRI
HCCCCCCEEECCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]