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The map label for this gene is capI [H]

Identifier: 78779696

GI number: 78779696

Start: 1223581

End: 1224648

Strand: Reverse

Name: capI [H]

Synonym: PMT9312_1313

Alternate gene names: 78779696

Gene position: 1224648-1223581 (Counterclockwise)

Preceding gene: 78779697

Following gene: 78779691

Centisome position: 71.65

GC content: 30.15

Gene sequence:

>1068_bases
ATGCTTATGAATTTAAGAGGTAAAAATATTCTTGTTACTGGCGCAGCAGGTTTTATCGGTTATGCTCTTGCCGAAAGATT
GTTAAAAGAAGGGGAAATAAATATCATCGGTATCGATAATTTGAACTCTTATTACAATCCAGCCTTAAAAAAAAGAAGAA
TTGAAATTCTTAATCAAAAAGATGAAAATAGGTTATGGCATTTTTTAAAAGTAGATTTAAAGGATAAAAATAAAGTAAAT
GAAATTTTTGAAAAATATAATCCCCATTTAGTGGTTAATTTGGCCGCCCAAGCGGGAGTAAGGTATTCCTTAGAAAATCC
TGATACTTATTTAGAAAGTAACCTTTTAGGTTTCCTAAATATATTAGAAGGTTGTAGAAACTTTAATGTTGAACATTTAA
TATATGCATCTAGTAGTTCTGTCTATGGTGGAAATATAATAATGCCTTACAGTGAAGATCATAGTGTAGATCATCCTTTG
AGCTTATATGCAGCAACAAAAAAATCTAATGAAATGTTGGCACATTCTTATAGTCATCTTTTTAAAATTCCTTCAACAGG
TTTAAGATTTTTTACAGTTTATGGACCTTATGGAAGACCAGATATGGCTCCAATGATTTTTGCGGATTCTATTTTAAATA
GAAAACCCATAAATGTTTTTAATAATGGAGATATGTCAAGAGACTTTACATTTATATCTGATATCGTGGAGGCTATTTAT
AAATGTTGTTTGAAAAAACCAATCTCAAATAAAAGCTTTTATGACCATCGACCAGAACCATCAACTTCTTTCGCTCCTCA
TAGGATTTTCAATGTAGGAAGTAATAATCCAATTAATCTCATGAGCTTTATTGAAAAATTAGAGGATGAATTAGGTATTA
GTGCTATAAAAAAAATGAGACCTATGCAACCTGGCGATGTTAAATCAACCTTCGCCGATATAACAAAACTAAGTGAATGG
ATAAATTACCATCCAAGTACGTCTTTTAATAAAGGTATTCATCTTTTCGCAAAGTGGTATAAAGATTATTTTAAGTCTGA
TTACTATAGAAAACCCTCTAAAAATTGA

Upstream 100 bases:

>100_bases
TTTTAATTTTTTTTAAATTAATTTTTTAAAAAAAATTTTTATTTTATTAACTCTAAGTTATAGTAAAAGAATTTATAAAT
TACCTATTAATAAACATTTA

Downstream 100 bases:

>100_bases
CTTTAAAGATTCAAAGTTGATCAGGTGAATAAGTTTAAATTTTTAACAAATATATGGTTTTTCTAGACCGTTTAAAAAAA
ACTAACTGCTAAATGACTTT

Product: putative nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLMNLRGKNILVTGAAGFIGYALAERLLKEGEINIIGIDNLNSYYNPALKKRRIEILNQKDENRLWHFLKVDLKDKNKVN
EIFEKYNPHLVVNLAAQAGVRYSLENPDTYLESNLLGFLNILEGCRNFNVEHLIYASSSSVYGGNIIMPYSEDHSVDHPL
SLYAATKKSNEMLAHSYSHLFKIPSTGLRFFTVYGPYGRPDMAPMIFADSILNRKPINVFNNGDMSRDFTFISDIVEAIY
KCCLKKPISNKSFYDHRPEPSTSFAPHRIFNVGSNNPINLMSFIEKLEDELGISAIKKMRPMQPGDVKSTFADITKLSEW
INYHPSTSFNKGIHLFAKWYKDYFKSDYYRKPSKN

Sequences:

>Translated_355_residues
MLMNLRGKNILVTGAAGFIGYALAERLLKEGEINIIGIDNLNSYYNPALKKRRIEILNQKDENRLWHFLKVDLKDKNKVN
EIFEKYNPHLVVNLAAQAGVRYSLENPDTYLESNLLGFLNILEGCRNFNVEHLIYASSSSVYGGNIIMPYSEDHSVDHPL
SLYAATKKSNEMLAHSYSHLFKIPSTGLRFFTVYGPYGRPDMAPMIFADSILNRKPINVFNNGDMSRDFTFISDIVEAIY
KCCLKKPISNKSFYDHRPEPSTSFAPHRIFNVGSNNPINLMSFIEKLEDELGISAIKKMRPMQPGDVKSTFADITKLSEW
INYHPSTSFNKGIHLFAKWYKDYFKSDYYRKPSKN
>Mature_355_residues
MLMNLRGKNILVTGAAGFIGYALAERLLKEGEINIIGIDNLNSYYNPALKKRRIEILNQKDENRLWHFLKVDLKDKNKVN
EIFEKYNPHLVVNLAAQAGVRYSLENPDTYLESNLLGFLNILEGCRNFNVEHLIYASSSSVYGGNIIMPYSEDHSVDHPL
SLYAATKKSNEMLAHSYSHLFKIPSTGLRFFTVYGPYGRPDMAPMIFADSILNRKPINVFNNGDMSRDFTFISDIVEAIY
KCCLKKPISNKSFYDHRPEPSTSFAPHRIFNVGSNNPINLMSFIEKLEDELGISAIKKMRPMQPGDVKSTFADITKLSEW
INYHPSTSFNKGIHLFAKWYKDYFKSDYYRKPSKN

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=357, Percent_Identity=23.249299719888, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI56118217, Length=357, Percent_Identity=23.249299719888, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI189083684, Length=357, Percent_Identity=23.249299719888, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI7657641, Length=282, Percent_Identity=26.9503546099291, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI42516563, Length=361, Percent_Identity=22.4376731301939, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=24.2339832869081, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994969, Length=367, Percent_Identity=23.9782016348774, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786974, Length=340, Percent_Identity=22.6470588235294, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=339, Percent_Identity=23.8938053097345, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=361, Percent_Identity=23.2686980609418, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=363, Percent_Identity=23.1404958677686, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=354, Percent_Identity=24.0112994350282, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=358, Percent_Identity=20.9497206703911, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=254, Percent_Identity=27.9527559055118, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19923002, Length=350, Percent_Identity=24.2857142857143, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21356223, Length=359, Percent_Identity=23.1197771587744, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 40727; Mature: 40727

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMNLRGKNILVTGAAGFIGYALAERLLKEGEINIIGIDNLNSYYNPALKKRRIEILNQK
CEEECCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCHHHHHHHHHHHCCC
DENRLWHFLKVDLKDKNKVNEIFEKYNPHLVVNLAAQAGVRYSLENPDTYLESNLLGFLN
CCHHEEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHH
ILEGCRNFNVEHLIYASSSSVYGGNIIMPYSEDHSVDHPLSLYAATKKSNEMLAHSYSHL
HHHHHHCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
FKIPSTGLRFFTVYGPYGRPDMAPMIFADSILNRKPINVFNNGDMSRDFTFISDIVEAIY
HCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
KCCLKKPISNKSFYDHRPEPSTSFAPHRIFNVGSNNPINLMSFIEKLEDELGISAIKKMR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHC
PMQPGDVKSTFADITKLSEWINYHPSTSFNKGIHLFAKWYKDYFKSDYYRKPSKN
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLMNLRGKNILVTGAAGFIGYALAERLLKEGEINIIGIDNLNSYYNPALKKRRIEILNQK
CEEECCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCHHHHHHHHHHHCCC
DENRLWHFLKVDLKDKNKVNEIFEKYNPHLVVNLAAQAGVRYSLENPDTYLESNLLGFLN
CCHHEEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHH
ILEGCRNFNVEHLIYASSSSVYGGNIIMPYSEDHSVDHPLSLYAATKKSNEMLAHSYSHL
HHHHHHCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
FKIPSTGLRFFTVYGPYGRPDMAPMIFADSILNRKPINVFNNGDMSRDFTFISDIVEAIY
HCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
KCCLKKPISNKSFYDHRPEPSTSFAPHRIFNVGSNNPINLMSFIEKLEDELGISAIKKMR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHC
PMQPGDVKSTFADITKLSEWINYHPSTSFNKGIHLFAKWYKDYFKSDYYRKPSKN
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]